FastQCFastQC Report
Thu 26 May 2016
SRR1926578_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926578_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714109
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT7410.10376567162716056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG851.529108E-615.7634791
GTCCAGA851.529108E-615.7634791
GTCCTAA1650.015.4291021
CTGTAGG5100.015.23802951
TAAAGGT856.2484105E-815.1119314
GTCTTAT550.004451420614.61704351
TGTAGGA5300.014.455252
GTCCTAC4750.014.3862491
TAGGACA1850.013.8866394
TCCTAGA1006.6540815E-613.6808622
TCTAGGA802.1270478E-413.68086052
GTAGGAC5600.013.6593943
GTTCTAG600.00792385913.3989581
GGTCTGG600.00792385913.3989581
GTACAAT802.5375705E-413.3989571
TAGGACC2250.013.3208884
TGGATTG550.001584082113.2008015
CGCCACC555.847306E-513.05390414
GACGTGG2350.012.96559057
GGCATCC450.00989657812.9566688
Produced by FastQC (version 0.11.5)