##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926578_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 714109 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.348236753772884 28.0 16.0 31.0 2.0 31.0 2 17.19794037044765 25.0 2.0 31.0 2.0 31.0 3 20.909085307705126 26.0 15.0 31.0 2.0 31.0 4 22.93957225017469 28.0 10.0 35.0 2.0 37.0 5 23.89526388828596 30.0 10.0 35.0 2.0 37.0 6 24.418580356780268 31.0 12.0 35.0 2.0 37.0 7 24.38673507825836 31.0 12.0 35.0 2.0 37.0 8 24.786423361139548 32.0 17.0 35.0 2.0 37.0 9 27.714759231433856 33.0 21.0 38.0 2.0 39.0 10 28.56109781559958 33.0 27.0 39.0 2.0 39.0 11 25.537647614019708 31.0 11.0 37.0 2.0 39.0 12 26.70952473641979 32.0 18.0 37.0 2.0 39.0 13 23.233647804466823 30.0 2.0 37.0 2.0 39.0 14 26.795518611304438 32.0 17.0 38.0 2.0 40.0 15 27.984260105950213 32.0 25.0 39.0 2.0 40.0 16 28.386777088651733 33.0 26.0 39.0 2.0 40.0 17 27.66927037749139 32.0 22.0 39.0 2.0 40.0 18 27.78670903181447 31.0 24.0 39.0 2.0 40.0 19 28.234087513250778 32.0 26.0 39.0 2.0 40.0 20 28.50345675520124 32.0 27.0 39.0 2.0 41.0 21 26.211117630501786 31.0 14.0 38.0 2.0 40.0 22 27.230957738944614 31.0 19.0 39.0 2.0 40.0 23 27.266232465912065 32.0 22.0 39.0 2.0 40.0 24 26.2635438007363 32.0 2.0 39.0 2.0 40.0 25 26.08405299471089 32.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.107480219404231 1101 2 10.249706055716938 1101 3 6.543908442329187 1101 4 7.863640053519351 1101 5 7.683018824597646 1101 6 7.33549911033796 1101 7 7.094861458541917 1101 8 5.557121673107375 1101 9 5.397037849215067 1101 10 4.847492778665941 1101 11 7.922084170121995 1101 12 5.491034134659632 1101 13 10.170094317423423 1101 14 7.519258312073401 1101 15 6.596054793697075 1101 16 6.130835139220938 1101 17 2.2708672416577507 1101 18 4.49820310972305 1101 19 5.225049405555346 1101 20 3.4094168532153653 1101 21 0.26827099411913835 1101 22 4.248104472953287 1101 23 5.417112722993256 1101 24 7.092387771597579 1101 25 7.590216674141484 1102 1 5.861340417666135 1102 2 11.135360318647464 1102 3 6.952405303386556 1102 4 8.113630182630576 1102 5 8.22178622969756 1102 6 6.856409056517791 1102 7 6.968345936739507 1102 8 7.123091886168808 1102 9 6.083752858275865 1102 10 5.420974397709539 1102 11 8.402825044995883 1102 12 5.689314421609772 1102 13 10.248022601672343 1102 14 7.72416843252547 1102 15 5.56221602547507 1102 16 6.120312771786974 1102 17 6.990464563855809 1102 18 6.677384248020331 1102 19 5.568838846563509 1102 20 6.040822889842492 1102 21 5.1555719935976825 1102 22 6.205068759396951 1102 23 6.376193464140432 1102 24 7.916515763668194 1102 25 7.604419181211082 1103 1 5.579475675164719 1103 2 10.254804291482778 1103 3 7.118230147755543 1103 4 8.361779588403074 1103 5 7.997830372312158 1103 6 7.224565536427242 1103 7 7.3253365988787245 1103 8 7.175267890696254 1103 9 6.097214940901786 1103 10 5.209176152095505 1103 11 6.275486335318465 1103 12 5.083908366148538 1103 13 9.585862160246197 1103 14 7.516263791891628 1103 15 6.495939851168334 1103 16 6.34341265860078 1103 17 3.8475245525371946 1103 18 2.8370744008216846 1103 19 4.9081441663145 1103 20 5.375303648189973 1103 21 7.49518291606514 1103 22 2.9689170899006285 1103 23 5.494219779929903 1103 24 7.163543211319578 1103 25 3.0077086548952927 1104 1 5.6239196740954895 1104 2 10.466225702870108 1104 3 6.777268506483157 1104 4 7.500368201073481 1104 5 7.574759001021061 1104 6 7.194360377378857 1104 7 7.010659373537909 1104 8 6.9404416410303895 1104 9 5.83649254047409 1104 10 4.74404450280387 1104 11 7.8571282759760415 1104 12 4.940111921347686 1104 13 9.539781566821976 1104 14 7.066170902289922 1104 15 6.301748458492579 1104 16 5.937571306021258 1104 17 6.145767001080365 1104 18 3.7363747215915346 1104 19 4.549828876285098 1104 20 3.6098980079868177 1104 21 2.684469871424671 1104 22 5.005121313370289 1104 23 5.342533733418275 1104 24 6.618450321717873 1104 25 5.6222936589049155 1105 1 3.6077235384125856 1105 2 9.939918899097385 1105 3 6.7841101011354965 1105 4 5.762882929506414 1105 5 2.2938909783727652 1105 6 4.898044557406262 1105 7 6.246313677355804 1105 8 3.1147434151717803 1105 9 4.2166496728365175 1105 10 3.7813187317380823 1105 11 7.555367842699976 1105 12 4.60034387508405 1105 13 5.828315540199576 1105 14 4.137217560345398 1105 15 4.904416929645418 1105 16 5.214028431716553 1105 17 5.882936362949003 1105 18 3.3069481381954233 1105 19 3.0290046946750273 1105 20 2.1675122728985983 1105 21 3.118809567191793 1105 22 4.796485077468613 1105 23 2.0773957706058965 1105 24 5.553521843798212 1105 25 6.197986432673073 1106 1 6.26517124214978 1106 2 11.297770860182265 1106 3 7.579689246457477 1106 4 8.994152843362663 1106 5 8.692973649314364 1106 6 7.234645747056682 1106 7 7.106852028709731 1106 8 6.752403575241932 1106 9 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1.2332898497572593 1107 23 -2.0573662415754725 1107 24 1.8500754693840946 1107 25 2.603004353820026 1108 1 4.963705535783667 1108 2 10.746903866252445 1108 3 0.021138841353465665 1108 4 5.8370878803945665 1108 5 7.184641144813931 1108 6 7.16149738145441 1108 7 7.223116542181348 1108 8 6.8789520390543935 1108 9 5.129883851839651 1108 10 4.15891687971185 1108 11 6.646143670541711 1108 12 2.006834777677007 1108 13 3.4483621602461945 1108 14 4.582323914735948 1108 15 4.403308147884612 1108 16 4.408784144544558 1108 17 -0.33168050297899043 1108 18 3.1436353505027625 1108 19 4.0687991391470035 1108 20 4.429645131771355 1108 21 4.7466812623916255 1108 22 1.536297198570601 1108 23 4.352488738115589 1108 24 5.17145662969898 1108 25 5.666133530869779 1109 1 4.021018885041265 1109 2 -14.667695708517222 1109 3 -6.01700647775886 1109 4 2.223425678938053 1109 5 2.5215752282792394 1109 6 3.7445552483613973 1109 7 4.61475017912564 1109 8 4.6042596602435815 1109 9 3.9242211137412895 1109 10 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4.3596139930175575 2208 21 7.035930042501924 2208 22 6.099865365762696 2208 23 1.5256565615391011 2208 24 3.1710432113195814 2208 25 4.898656930757362 2209 1 3.4836423418313878 2209 2 5.339804291482778 2209 3 5.623230147755542 2209 4 1.255112921736405 2209 5 5.572830372312161 2209 6 5.982065536427243 2209 7 6.5078365988787255 2209 8 5.027767890696257 2209 9 4.6322149409017825 2209 10 3.7666761520955028 2209 11 -0.6061803313481988 2209 12 3.668908366148539 2209 13 2.791695493579528 2209 14 3.90876379189163 2209 15 4.43427318450167 2209 16 4.070912658600783 2209 17 1.345857885870526 2209 18 3.7329077341550203 2209 19 4.391477499647834 2209 20 4.228636981523305 2209 21 6.629349582731805 2209 22 5.431417089900627 2209 23 0.2808864465965719 2209 24 -3.711456788680419 2209 25 -0.23145801177137315 2210 1 -1.8982719482636767 2210 2 -14.667695708517222 2210 3 -7.888987730196005 2210 4 -1.8845681576870845 2210 5 -0.1290969505949242 2210 6 -0.7914410566423697 2210 7 -0.9397990956934912 2210 8 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-5.33685940836839 2211 21 -2.840379659145448 2211 22 -4.49764127351694 2211 23 -6.47013942584627 2211 24 -23.745623455347086 2211 25 -23.579791345104706 2212 1 -0.3331105317318297 2212 2 -14.667695708517222 2212 3 -7.821568702819169 2212 4 -20.578220411596927 2212 5 -21.31216962768784 2212 6 -21.789601130239426 2212 7 -10.056301332155757 2212 8 -9.212414101640908 2212 9 -4.555514944155686 2212 10 -5.137404307674611 2212 11 -5.170404469279234 2212 12 -2.5062927832767485 2212 13 -6.484588031324687 2212 14 -3.6350676257328907 2212 15 -3.546665512816336 2212 16 -3.4719225904413626 2212 17 -3.789544412980046 2212 18 -2.8940271317453643 2212 19 -3.4639152206586807 2212 20 -3.621947309664435 2212 21 -1.5332749383409876 2212 22 -2.9315043660380695 2212 23 -3.237035009342126 2212 24 -1.9027115779524522 2212 25 -2.2301744868671562 2213 1 -19.922190991501946 2213 2 -14.667695708517222 2213 3 -9.019689516513042 2213 4 -5.996385879222828 2213 5 -21.31216962768784 2213 6 -11.044097533117123 2213 7 -21.832163401121274 2213 8 -11.176218919855303 2213 9 -8.823138776124594 2213 10 -7.566908979798981 2213 11 -6.987085607127575 2213 12 -5.657392593084076 2213 13 -20.551637839753806 2213 14 -13.360852515062806 2213 15 -7.596422259143413 2213 16 -6.824075350991546 2213 17 -5.401995831155851 2213 18 -5.503267325797019 2213 19 -6.48466758428502 2213 20 -6.479078845814822 2213 21 -4.725362647484019 2213 22 -5.37843303000345 2213 23 -5.185762234458586 2213 24 -4.113429210742769 2213 25 -5.010546740788158 2214 1 -19.922190991501946 2214 2 -14.667695708517222 2214 3 -12.347953237824393 2214 4 -20.578220411596927 2214 5 -21.31216962768784 2214 6 -21.789601130239426 2214 7 -17.06413832275137 2214 8 -14.994894073776054 2214 9 -13.58529550841901 2214 10 -13.49215874863595 2214 11 -14.598826621839319 2214 12 -11.928445343360343 2214 13 -20.551637839753806 2214 14 -17.3093971276486 2214 15 -25.507393482164996 2214 16 -18.87883655770016 2214 17 -25.26080878079614 2214 18 -18.55477251662868 2214 19 -15.141989062525626 2214 20 -13.566914220148586 2214 21 -11.030749685815737 2214 22 -12.213026483767084 2214 23 -12.091963605650033 2214 24 -10.779061281888362 2214 25 -10.58292614134295 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 103582.0 3 4.0 4 405.0 5 1518.0 6 2367.0 7 2459.0 8 2059.0 9 1883.0 10 1727.0 11 1633.0 12 1562.0 13 1846.0 14 2945.0 15 7121.0 16 11244.0 17 11700.0 18 11691.0 19 13178.0 20 15394.0 21 18113.0 22 18604.0 23 20986.0 24 22516.0 25 23348.0 26 19054.0 27 14587.0 28 15585.0 29 18455.0 30 18181.0 31 21023.0 32 27624.0 33 36114.0 34 44946.0 35 52804.0 36 52948.0 37 55253.0 38 39650.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.29810680754031 18.314709772703296 11.667356428171482 25.71982699158491 2 13.858801458355458 19.9970797493894 42.3690754309582 23.775043361296948 3 19.180335938164227 23.960466632160983 29.530399554906545 27.32879787476824 4 11.35034125476374 17.103973478507744 38.75773775336387 32.78794751336465 5 12.386675168389129 37.21844787462333 37.54211751149836 12.852759445489179 6 31.468544148967737 37.393427273386656 18.278088053963877 12.859940523681729 7 27.65498577389838 31.723820653860347 22.271452235651555 18.34974133658972 8 24.325322750397312 38.99256038093745 20.320533891430415 16.361582977234825 9 26.017733852030982 15.74752041495812 20.231871867662004 38.002873865348896 10 13.506348462422771 30.144832979809994 35.568056685294906 20.780761872472333 11 33.49668595570235 22.69601941733089 24.77097067261002 19.03632395435674 12 22.841129090083413 24.927250890317644 32.63895528095796 19.59266473864098 13 28.236712505177163 21.811160539867096 25.48858173379233 24.463545221163407 14 19.78617482597284 22.584303320780556 28.235478717334246 29.394043135912355 15 22.06658966155821 32.07354325176478 23.666349104924038 22.19351798175297 16 21.31101942422697 28.80603588150758 28.54845626072041 21.334488433545037 17 20.784690654968816 28.89566606202077 29.239374733510147 21.080268549500264 18 20.6242285512299 28.15741688307616 31.02955806853458 20.18879649715936 19 22.31322774539792 27.43538710748229 27.597515924383575 22.653869222736216 20 22.341365433642117 28.432792319523553 28.769620822125614 20.45622142470872 21 21.381993445264726 26.939256218397073 27.22435338521641 24.45439695112179 22 21.807329974379897 28.918620508636085 28.277832619127775 20.996216897856243 23 21.44377185186016 28.418843558204802 28.598144466905733 21.539240123029302 24 21.89722211952034 27.793439814643644 29.034335292885228 21.275002772950792 25 21.842356507073532 28.003499263276098 28.85840741462485 21.295736815025524 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 820.0 1 820.0 2 1778.0 3 2736.0 4 2736.0 5 2736.0 6 3881.0 7 5026.0 8 5026.0 9 5026.0 10 5886.0 11 6746.0 12 6746.0 13 6746.0 14 8049.5 15 9353.0 16 9353.0 17 9353.0 18 13027.5 19 16702.0 20 16702.0 21 16702.0 22 23078.0 23 29454.0 24 29454.0 25 29454.0 26 38476.5 27 47499.0 28 47499.0 29 47499.0 30 57132.0 31 66765.0 32 66765.0 33 66765.0 34 73599.0 35 80433.0 36 80433.0 37 80433.0 38 84062.5 39 87692.0 40 87692.0 41 87692.0 42 88173.0 43 88654.0 44 88654.0 45 88654.0 46 86579.0 47 84504.0 48 84504.0 49 84504.0 50 77567.5 51 70631.0 52 70631.0 53 70631.0 54 60241.5 55 49852.0 56 49852.0 57 49852.0 58 41209.0 59 32566.0 60 32566.0 61 32566.0 62 25783.0 63 19000.0 64 19000.0 65 19000.0 66 14279.5 67 9559.0 68 9559.0 69 9559.0 70 6781.0 71 4003.0 72 4003.0 73 4003.0 74 2705.5 75 1408.0 76 1408.0 77 1408.0 78 969.0 79 530.0 80 530.0 81 530.0 82 337.0 83 144.0 84 144.0 85 144.0 86 85.0 87 26.0 88 26.0 89 26.0 90 16.0 91 6.0 92 6.0 93 6.0 94 3.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.716953574314287 2 36.70210009956463 3 6.11783355202077 4 14.42104776721761 5 13.47063263451378 6 15.051063633142839 7 9.292138875157715 8 7.128183512601018 9 0.10978716134371644 10 1.6983401693578992 11 6.130716739321309 12 6.257448092658123 13 16.487679051797414 14 1.8301127698992732 15 3.1339753455004766 16 3.338005822640521 17 3.114930633838812 18 1.7514132996503335 19 1.7081425944778739 20 0.3044353172974994 21 4.759777568970564 22 1.834033739947263 23 0.10964712669914536 24 9.099591238872497 25 0.11426826996999058 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 714109.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.60693280267131 #Duplication Level Percentage of deduplicated Percentage of total 1 97.1686313192726 90.95657542430693 2 2.110072025880063 3.950347406707032 3 0.3309691651096947 0.9294302519453826 4 0.1344004241028944 0.5032324587060065 5 0.07060005451214213 0.33043272792915096 6 0.039925712848694596 0.22423941118359028 7 0.03247676984237724 0.212803556859826 8 0.015468866968328089 0.11583945526701997 9 0.014432984231180846 0.12159246465631443 >10 0.07232951419431483 1.2480507262514797 >50 0.005805022691663488 0.3902345408324822 >100 0.00442987719104654 0.754980309618262 >500 4.5826315497679896E-4 0.2622412657365569 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 741 0.10376567162716056 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 1.4003464457106689E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAG 85 1.529108E-6 15.763479 1 GTCCAGA 85 1.529108E-6 15.763479 1 GTCCTAA 165 0.0 15.429102 1 CTGTAGG 510 0.0 15.2380295 1 TAAAGGT 85 6.2484105E-8 15.111931 4 GTCTTAT 55 0.0044514206 14.6170435 1 TGTAGGA 530 0.0 14.45525 2 GTCCTAC 475 0.0 14.386249 1 TAGGACA 185 0.0 13.886639 4 TCCTAGA 100 6.6540815E-6 13.680862 2 TCTAGGA 80 2.1270478E-4 13.6808605 2 GTAGGAC 560 0.0 13.659394 3 GTTCTAG 60 0.007923859 13.398958 1 GGTCTGG 60 0.007923859 13.398958 1 GTACAAT 80 2.5375705E-4 13.398957 1 TAGGACC 225 0.0 13.320888 4 TGGATTG 55 0.0015840821 13.200801 5 CGCCACC 55 5.847306E-5 13.053904 14 GACGTGG 235 0.0 12.9655905 7 GGCATCC 45 0.009896578 12.956668 8 >>END_MODULE