FastQCFastQC Report
Thu 26 May 2016
SRR1926578_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926578_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714109
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA13260.18568593870123468No Hit
GTCCTACAGTGGACATTTCTAAATT13060.18288524580981333No Hit
GTATCAACGCAGAGTACTTTTTTTT11960.167481434906996No Hit
GTCCTACAGTGTGCATTTCTCATTT11780.1649608113047168No Hit
CTGTAGGACCTGGAATATGGCGAGA10520.14731644608876235No Hit
GTCCTAAAGTGTGTATTTCTCATTT9350.13093239267394755No Hit
CTTTAGGACGTGAAATATGGCGAGG9210.12897190764995262No Hit
GGTATCAACGCAGAGTACTTTTTTT7280.1019452212477367No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGTC350.002107644216.355414
TAGGACC3300.014.1664284
GAACTAC655.2547817E-513.2036211
TGTAGGA8100.013.1929482
TCCAACG957.649032E-812.96239418
GTAGGAC7650.012.8455883
GGGCTAC751.4196856E-512.7145981
GTGCTAG751.4196856E-512.7145981
TGCCGGT604.1611813E-412.63975810
GCCGGTT604.1636417E-412.63887311
GTCCTAG1301.8553692E-1012.4700861
GTTCTAA1155.013135E-912.4381931
TAGGCAG701.04671424E-412.2665585
GTCCTAC8100.012.1259591
CTGTAGG8800.011.8115721
CCAACGA658.211231E-411.65848419
TAGAACT1001.8281426E-611.4487884
TAGCCCT1604.1836756E-1111.3295294
TCCTACA8950.011.3003442
GGACCTG5550.011.1737416
Produced by FastQC (version 0.11.5)