##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926578_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 714109 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.239590874782422 31.0 30.0 31.0 25.0 34.0 2 29.605770267564196 31.0 30.0 33.0 25.0 34.0 3 29.900666424873513 31.0 30.0 34.0 26.0 34.0 4 33.70435465734223 35.0 35.0 37.0 28.0 37.0 5 33.55412689099283 35.0 35.0 37.0 28.0 37.0 6 33.678037946587985 36.0 35.0 37.0 29.0 37.0 7 33.54742483290366 36.0 35.0 37.0 28.0 37.0 8 33.56441103529013 37.0 35.0 37.0 28.0 37.0 9 34.89505103562622 39.0 35.0 39.0 28.0 39.0 10 34.83062109565907 38.0 35.0 39.0 28.0 39.0 11 35.08922167344201 39.0 35.0 39.0 29.0 39.0 12 34.89050271037055 38.0 35.0 39.0 28.0 39.0 13 35.05889997185304 39.0 35.0 39.0 29.0 39.0 14 35.816084099206144 39.0 36.0 41.0 27.0 41.0 15 35.964754680307905 39.0 36.0 41.0 28.0 41.0 16 35.963387942176894 39.0 36.0 41.0 28.0 41.0 17 35.87675690965945 39.0 36.0 41.0 27.0 41.0 18 35.86154634656614 39.0 36.0 41.0 27.0 41.0 19 35.911394478994104 39.0 36.0 41.0 27.0 41.0 20 35.92118290064962 39.0 36.0 41.0 27.0 41.0 21 35.858091691884574 39.0 36.0 41.0 27.0 41.0 22 35.78683926403392 39.0 36.0 41.0 27.0 41.0 23 35.48026001632804 39.0 36.0 41.0 27.0 41.0 24 35.58592315738914 39.0 36.0 41.0 27.0 41.0 25 35.402490376119054 39.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5520473049267132 1101 2 0.6861970392917875 1101 3 0.5768258346517285 1101 4 0.33572620527355923 1101 5 0.2917404162678139 1101 6 0.23410119681783925 1101 7 0.31558513610713845 1101 8 0.3700604183655827 1101 9 0.6778623557307029 1101 10 0.6597333358695963 1101 11 0.4491966645075749 1101 12 0.7224807923211074 1101 13 0.6276583705677581 1101 14 0.8134814022186916 1101 15 0.6060226106591458 1101 16 0.539292006262194 1101 17 0.5305854886905195 1101 18 0.6523203997783469 1101 19 0.6531876160755132 1101 20 0.5158414820269215 1101 21 0.6355496048237228 1101 22 0.6227064779582676 1101 23 0.5566897756073317 1101 24 0.6148293687845978 1101 25 0.6120070959927375 1102 1 0.4495398268775297 1102 2 0.5377266186013152 1102 3 0.5297056153954642 1102 4 0.16307142633381488 1102 5 0.4227094107850462 1102 6 0.07020087509494033 1102 7 0.42193613401995833 1102 8 0.47658568852159533 1102 9 0.7038779340354466 1102 10 0.6787399023932537 1102 11 0.5524114427937974 1102 12 0.455844940980775 1102 13 0.6266161408633266 1102 14 0.821653136622345 1102 15 0.7422000306947396 1102 16 0.5874394787019952 1102 17 0.6920289802349089 1102 18 0.6093874796154566 1102 19 0.6814646490361085 1102 20 0.5130218115920684 1102 21 0.7395397584419854 1102 22 0.6965291532278144 1102 23 0.9128641186486277 1102 24 0.8424942704909668 1102 25 0.9893602818391116 1103 1 0.38750106060701484 1103 2 0.15033697513781874 1103 3 0.1252333460123296 1103 4 -0.032826989059508094 1103 5 0.09755236494464015 1103 6 -0.06854983231875877 1103 7 0.11223041811729928 1103 8 0.14242609599187972 1103 9 0.4413934971367439 1103 10 0.4852806494221156 1103 11 0.15576175405582404 1103 12 0.3239957615806617 1103 13 0.0861039733477611 1103 14 0.41733932790700834 1103 15 0.3013921642544446 1103 16 0.5889264622899972 1103 17 0.48347776348335714 1103 18 0.4040592396607252 1103 19 0.40376232871918916 1103 20 0.3323497685813166 1103 21 0.20199306913808357 1103 22 0.17657442770432397 1103 23 0.5616770784675253 1103 24 0.30031119182656596 1103 25 0.42556429994890266 1104 1 0.5007241293052225 1104 2 0.6934143608635601 1104 3 0.5872508546998461 1104 4 0.32209348674909677 1104 5 0.2508422606944407 1104 6 -0.05459166605304944 1104 7 0.11029243362117569 1104 8 0.32515263969677477 1104 9 0.683475828064303 1104 10 0.7134622853483421 1104 11 0.3994773381242567 1104 12 0.3776532061142106 1104 13 0.43118683889173326 1104 14 0.9049008087944728 1104 15 0.7487651928564247 1104 16 0.7429808595099914 1104 17 0.5778990412165825 1104 18 0.45023539576872196 1104 19 0.617902932835733 1104 20 0.47333947721537584 1104 21 0.7397998053048838 1104 22 0.5713833023367769 1104 23 0.721886247139004 1104 24 0.48892720358812625 1104 25 0.803667079954252 1105 1 0.8678604234663752 1105 2 0.7177942400950812 1105 3 0.8316066948856786 1105 4 0.6692340055014867 1105 5 0.9300717899640674 1105 6 0.5769610456921228 1105 7 0.6633182192050953 1105 8 0.5177640913298802 1105 9 1.1161487418919904 1105 10 1.1835265701680413 1105 11 0.8427703010643697 1105 12 0.8406060956910011 1105 13 0.7342663665101554 1105 14 1.2619566550243349 1105 15 1.0326334229957013 1105 16 1.1129921188556509 1105 17 1.0286292786348739 1105 18 1.0145604051618946 1105 19 1.1991780241348806 1105 20 1.315137258868809 1105 21 1.151522983667995 1105 22 1.1749116460415436 1105 23 1.4335865578770068 1105 24 1.047815076830453 1105 25 1.1809081227927223 1106 1 0.7611852389072844 1106 2 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0.5819641884015212 1107 14 0.9298214067600554 1107 15 0.7824943147920749 1107 16 0.6729139174871293 1107 17 0.7249472975335323 1107 18 0.7881273400191517 1107 19 0.6507157337371083 1107 20 0.6442046072909946 1107 21 0.7093945028298378 1107 22 0.5888324922204546 1107 23 0.7893025264962006 1107 24 0.8158577868086425 1107 25 0.9050177049668235 1108 1 0.9042450955703956 1108 2 0.8029432827210918 1108 3 0.6603851296173389 1108 4 0.36330991148384584 1108 5 -0.8663154592934887 1108 6 0.18772881166896127 1108 7 0.5579544908789345 1108 8 0.4813204971258287 1108 9 0.8255631167918338 1108 10 0.7475432291528037 1108 11 0.51097555041774 1108 12 0.713913668502471 1108 13 0.4854460475268283 1108 14 0.9201925053225537 1108 15 0.7223920461349067 1108 16 0.7155807264052783 1108 17 0.7060126087467609 1108 18 0.7557448054533111 1108 19 0.8111093639188098 1108 20 0.8144552493278354 1108 21 0.9060221265441726 1108 22 0.8349113046237733 1108 23 1.2643589825544623 1108 24 1.072331626506461 1108 25 0.9784281081227775 1109 1 0.7667294556687416 1109 2 -0.27555808659057845 1109 3 0.6308814941604766 1109 4 0.7693232167018067 1109 5 -0.530338581557416 1109 6 0.29565798661128895 1109 7 0.7346584016563895 1109 8 0.756170951958957 1109 9 1.1290066658610272 1109 10 -1.0294004205367315 1109 11 0.4159263631093211 1109 12 0.8669895887411556 1109 13 0.7081821626481712 1109 14 1.185045089223884 1109 15 1.1664847568470336 1109 16 1.0226816063229194 1109 17 1.262448956898993 1109 18 1.1865077993315083 1109 19 1.1514989542335883 1109 20 1.0989238426553953 1109 21 1.3267050032944567 1109 22 1.3541052919018597 1109 23 1.3953293418420145 1109 24 1.1644572823615462 1109 25 1.4494223246402598 1110 1 0.8163563719623248 1110 2 -0.1980505988266792 1110 3 0.2719697506333496 1110 4 0.7420159244372542 1110 5 1.0206905728905369 1110 6 0.6378061834603344 1110 7 0.7934786570578467 1110 8 1.0735672625176633 1110 9 1.2917936098445537 1110 10 1.0801940052970167 1110 11 0.9159364511535983 1110 12 1.0742613293462284 1110 13 0.9477537338436761 1110 14 1.420328198011262 1110 15 1.3253151002927623 1110 16 0.8027211932001066 1110 17 1.1201275239792707 1110 18 1.1857738071445283 1110 19 1.2896174991897453 1110 20 1.3162557984488856 1110 21 1.2640429423418027 1110 22 1.2434157914687702 1110 23 1.4272680900200783 1110 24 1.420066756774439 1110 25 1.2966385462154548 1111 1 0.1357126710939056 1111 2 0.28205045828388364 1111 3 0.4692501999449128 1111 4 0.20800634427382647 1111 5 0.5252770840457615 1111 6 0.29520484932918123 1111 7 0.5004326653083098 1111 8 0.6228006278270897 1111 9 0.9208316993839389 1111 10 0.7841289640288593 1111 11 0.42534976903709776 1111 12 0.5327129900450842 1111 13 0.5051489171679862 1111 14 0.6726670432628126 1111 15 0.5439296174379606 1111 16 0.6122317057356881 1111 17 0.5831406848316689 1111 18 0.671531149773088 1111 19 0.4041368605543951 1111 20 0.16717186595959532 1111 21 0.5231353912354564 1111 22 0.6809189969927161 1111 23 0.897248239516216 1111 24 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0.737139594947692 2202 23 0.9469683126082415 2202 24 0.8923382967861997 2202 25 0.8791138962580121 2203 1 -0.016869225969156076 2203 2 0.19853833260388143 2203 3 -0.25964976107665905 2203 4 -0.6659001413973655 2203 5 -0.2958526124309273 2203 6 -0.2923017176882894 2203 7 -0.04576354870020083 2203 8 -0.011510555591826233 2203 9 0.2572155182528846 2203 10 0.1578032738565085 2203 11 0.03935149764556911 2203 12 0.10438414770434434 2203 13 -0.11349632831136347 2203 14 0.46907763861590723 2203 15 0.33256486410361674 2203 16 0.26879448642272763 2203 17 0.2561625296975336 2203 18 0.2484559214103541 2203 19 0.21658284153970442 2203 20 0.05320572634904153 2203 21 0.32025098769011606 2203 22 0.33787156194264156 2203 23 0.3801197632337434 2203 24 0.0020268781010770454 2203 25 0.1999949183501073 2204 1 0.7168630509121172 2204 2 0.7111774370710933 2204 3 0.5257900896678755 2204 4 0.29942350994248557 2204 5 0.4879437250815073 2204 6 0.2638204271622797 2204 7 0.538306836708685 2204 8 0.7482601421852095 2204 9 1.0404838877840206 2204 10 0.7212921977540248 2204 11 0.574917727822573 2204 12 0.6085773127640124 2204 13 0.5073429240178484 2204 14 0.7249733455859371 2204 15 0.6598345651669035 2204 16 0.7355824360567453 2204 17 0.6343788185161472 2204 18 0.7213147286826853 2204 19 0.7059294231018498 2204 20 0.7484311771926642 2204 21 0.7357099211483487 2204 22 0.7783594291300489 2204 23 0.9390644465884321 2204 24 0.622748460147065 2204 25 0.695674793248017 2205 1 0.800824417882886 2205 2 0.8008018594155502 2205 3 0.8509163734132343 2205 4 0.5440531652368463 2205 5 0.6471114232048123 2205 6 0.6313790051380153 2205 7 0.6087055444806921 2205 8 0.5806862715704924 2205 9 1.1113509326249087 2205 10 0.8876808888474983 2205 11 0.773282371241244 2205 12 0.9177753572177991 2205 13 0.7747299376999806 2205 14 1.1596331560527915 2205 15 1.1096197513786805 2205 16 1.0209383005448487 2205 17 1.2527701284740473 2205 18 1.234268071796933 2205 19 1.1281305117465905 2205 20 1.2810881298476104 2205 21 1.2952532447166902 2205 22 1.2116954705348704 2205 23 1.3638605049107326 2205 24 1.1473815562851968 2205 25 1.2835764042319582 2206 1 0.09175905147459318 2206 2 0.15070607711650652 2206 3 0.321393924398933 2206 4 0.08639295005769299 2206 5 0.4578681944727947 2206 6 0.07490146144075993 2206 7 0.06466573014164823 2206 8 0.08178682658549263 2206 9 0.27550308617784225 2206 10 0.3190972399852825 2206 11 0.15040406760224556 2206 12 0.2606814541225191 2206 13 0.060282055539545354 2206 14 0.4843066034016843 2206 15 0.2731311292468348 2206 16 0.20832904980902356 2206 17 0.35822662193084653 2206 18 0.32242301439436716 2206 19 0.3979784626613494 2206 20 0.1208923865417475 2206 21 0.1928530386966827 2206 22 0.21608736529945816 2206 23 0.4366732732924916 2206 24 0.38959962409445126 2206 25 0.4783078311513407 2207 1 -0.8268171212111675 2207 2 0.027079399380241398 2207 3 0.23955152783051048 2207 4 0.28679422306170466 2207 5 0.27399175888402993 2207 6 0.44819259192367156 2207 7 0.513745569632448 2207 8 0.5754563990221868 2207 9 0.7835147092579575 2207 10 0.5620230736645411 2207 11 0.5139435722376433 2207 12 0.46634424642915207 2207 13 0.33428579152958093 2207 14 -5.431827338759657 2207 15 -2.140501775139498 2207 16 -0.6708462649827354 2207 17 -0.5994010243954335 2207 18 -0.10472863912715269 2207 19 0.28179263174948943 2207 20 0.33394067767222424 2207 21 0.6013870085320221 2207 22 0.29899867012856873 2207 23 0.6337225330129854 2207 24 0.21053846455383507 2207 25 0.1323067241913236 2208 1 0.466207957158737 2208 2 0.8213714578964364 2208 3 0.6005494379663503 2208 4 0.6919143902508367 2208 5 0.9799086867837161 2208 6 0.8095823515892917 2208 7 0.868695935358673 2208 8 0.9175410385206177 2208 9 1.0315946465620343 2208 10 0.9955392701117773 2208 11 0.6987789954351342 2208 12 0.8671279454887113 2208 13 0.8030292607040863 2208 14 1.064868063539194 2208 15 0.9305013596567449 2208 16 0.7955069220601061 2208 17 0.6894835106097901 2208 18 0.7800937224193518 2208 19 0.6280439379145903 2208 20 0.8119187340985619 2208 21 0.8418781266093447 2208 22 0.9026951173594995 2208 23 1.1898092623755758 2208 24 1.118270961941505 2208 25 0.9658229206385585 2209 1 -0.259998939392986 2209 2 -0.0479963581955154 2209 3 0.5694000126789973 2209 4 0.549673010940495 2209 5 0.5700523649446367 2209 6 0.45145016768124435 2209 7 0.8247304181172979 2209 8 0.9257594293252183 2209 9 1.0497268304700782 2209 10 0.758613982755449 2209 11 0.8007617540558201 2209 12 0.8223290949139965 2209 13 0.5952706400144265 2209 14 1.1123393279070086 2209 15 0.9388921642544403 2209 16 0.889759795623327 2209 17 0.967644430150024 2209 18 0.916559239660728 2209 19 0.9420956620525232 2209 20 0.884016435247986 2209 21 0.9869930691380802 2209 22 0.9615744277043277 2209 23 0.9900104118008599 2209 24 1.1744778584932334 2209 25 1.083897633282234 2210 1 -0.07978888112864979 2210 2 -0.5766018473092842 2210 3 0.3655913650249012 2210 4 0.6955255101738587 2210 5 0.8086402214303803 2210 6 0.7115229981013584 2210 7 0.9658796054831384 2210 8 1.0762439432779942 2210 9 1.4144784404056807 2210 10 1.3694963807928673 2210 11 0.9725480159386777 2210 12 0.7840179940247012 2210 13 0.7363814955802042 2210 14 1.0722204993268178 2210 15 0.7364803273945881 2210 16 0.8618235797386333 2210 17 0.7026835285860855 2210 18 0.7049316714301241 2210 19 0.6635292713748129 2210 20 1.0017893576644212 2210 21 0.9407649182641151 2210 22 1.0608369238711504 2210 23 1.030227369789209 2210 24 0.9929246539547449 2210 25 1.0345439074312708 2211 1 -0.98928293457951 2211 2 -0.06882367468167772 2211 3 0.6900137310905485 2211 4 0.8431447317587839 2211 5 1.0086624732478882 2211 6 0.7302377729760678 2211 7 1.1209548465168169 2211 8 0.9502239299268993 2211 9 1.2814416319141202 2211 10 1.4550189165701326 2211 11 0.932645027220687 2211 12 1.0937400455758493 2211 13 1.0700450082454722 2211 14 1.4757538886771684 2211 15 1.0954926456022207 2211 16 1.3505299520613576 2211 17 1.2938628417023708 2211 18 1.2239088184814264 2211 19 1.3536419917757456 2211 20 1.3811072896402834 2211 21 1.3164154518095614 2211 22 1.3575972917235788 2211 23 1.5730098101161474 2211 24 1.3652780991671207 2211 25 1.5948272361703246 2212 1 0.9743497196108457 2212 2 -0.05961513214187519 2212 3 0.41376782877095053 2212 4 0.358581056917501 2212 5 0.7627152001936821 2212 6 0.6932030412444661 2212 7 0.7856020656268754 2212 8 0.7944567473328803 2212 9 1.0878685929221845 2212 10 0.6650794999968284 2212 11 0.8244973862397273 2212 12 0.7210551485538446 2212 13 0.6284602951868408 2212 14 0.8993987148801921 2212 15 0.7441986776644072 2212 16 0.2045107534777344 2212 17 0.7318685680810546 2212 18 0.7609366342967405 2212 19 0.4692795700985002 2212 20 0.6055011095774887 2212 21 0.7638129925097275 2212 22 0.7037008644859384 2212 23 0.8516866570115909 2212 24 0.7796694293744579 2212 25 0.2440796256193991 2213 1 -0.1328886276423873 2213 2 -0.09032849248808361 2213 3 -0.8281179729325139 2213 4 -1.4931926964935585 2213 5 -2.43109871419205 2213 6 -1.1613615829182748 2213 7 -0.7579734188131582 2213 8 -0.6700199471735857 2213 9 -1.2628990688104977 2213 10 -1.22462942252033 2213 11 -1.1706651044573704 2213 12 -1.3502188667166948 2213 13 -1.3843156909208219 2213 14 -8.750712230845988 2213 15 -4.011479538383448 2213 16 -2.8634176624102423 2213 17 -2.1256109655334328 2213 18 -6.136852031322487 2213 19 -4.034606975837168 2213 20 -5.997392437653694 2213 21 -3.15869038409933 2213 22 -2.739768497955147 2213 23 -5.103976398750696 2213 24 -3.118963388509165 2213 25 -2.5244297048472575 2214 1 0.06090492685570581 2214 2 -0.07589865704608911 2214 3 -1.6186250656909102 2214 4 -2.1257449618912716 2214 5 -0.707068847176572 2214 6 -0.6646861959551202 2214 7 -0.7406953917050672 2214 8 -0.9237599019182525 2214 9 -5.422232417178822 2214 10 -1.937846309825531 2214 11 -1.2517826555575553 2214 12 -1.5906829218049126 2214 13 -1.579481188616711 2214 14 -2.780939146492152 2214 15 -2.2365571565397033 2214 16 -1.6397072890161724 2214 17 -1.9640561968092243 2214 18 -1.5239815126903693 2214 19 -4.732392321228566 2214 20 -2.481315330687231 2214 21 -4.192474015501418 2214 22 -6.577385864876653 2214 23 -6.553659912128083 2214 24 -5.541473029699034 2214 25 -3.2699398797794146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15711.0 3 15.0 4 248.0 5 1620.0 6 2175.0 7 1971.0 8 1370.0 9 1139.0 10 1011.0 11 924.0 12 878.0 13 881.0 14 914.0 15 896.0 16 959.0 17 1035.0 18 1234.0 19 1414.0 20 1838.0 21 2396.0 22 3096.0 23 3955.0 24 4649.0 25 5555.0 26 6654.0 27 7313.0 28 8662.0 29 10839.0 30 13430.0 31 17253.0 32 22808.0 33 30382.0 34 42012.0 35 58391.0 36 83795.0 37 131721.0 38 224965.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.559577351678556 19.609502999995794 9.273147479821333 29.55777216850432 2 13.874876903875059 21.41599416142647 39.611247378735435 25.097881555963035 3 18.912906745934464 24.247653974760006 29.056953185957607 27.78248609334792 4 12.074218405279224 17.29797042413919 37.813649944739616 32.81416122584197 5 12.912976427760029 38.448986109340794 35.61804819219731 13.019989270701874 6 32.12501001547655 38.30368049434987 17.201879676524705 12.36942981364888 7 28.297709345298728 31.559289596785284 21.542480798965094 18.600520258950894 8 24.798496865194963 38.886737276957355 19.740712052581657 16.574053805266022 9 25.918769146140043 15.556173003223359 19.750747602829595 38.77431024780701 10 14.04127959171383 30.557457959213398 34.37169559487345 21.029566854199317 11 34.900482986252605 22.83380875760924 23.881982750290927 18.383725505847227 12 22.773179851279252 25.28980940777773 32.038538559565325 19.898472181377695 13 29.167396013773804 22.19633137238153 25.570046029387672 23.066226584456995 14 20.072345148866752 22.924386565833803 27.618552424305282 29.384715860994167 15 22.433552723708164 32.12141156700742 22.90813611539 22.536899593894415 16 21.41046646781218 29.262838017616826 28.132448290831686 21.194247223739303 17 21.023672550260756 29.599370375142144 28.068861017594767 21.308096057002334 18 20.91882747687947 28.539779633768575 30.02952033654304 20.511872552808914 19 22.963287643608947 27.59308324417582 27.353898377239904 22.089730734975323 20 22.849384818330257 28.300317323392587 28.34806987276256 20.502227985514594 21 22.06938175931618 27.450088012020608 27.38847259584348 23.09205763281973 22 22.276291154431608 28.961545086254336 27.539773339924295 21.222390419389757 23 21.61392728561046 28.60949798980268 27.91716670704332 21.85940801754354 24 22.4114559702454 27.666823505688214 28.771138259198885 21.1505822648675 25 21.987119613392352 28.20801866381743 28.265432868091565 21.539428854698652 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 143.0 1 143.0 2 143.0 3 143.0 4 143.0 5 143.0 6 292.5 7 442.0 8 442.0 9 442.0 10 856.0 11 1270.0 12 1270.0 13 1270.0 14 2055.5 15 2841.0 16 2841.0 17 2841.0 18 5059.0 19 7277.0 20 7277.0 21 7277.0 22 11938.0 23 16599.0 24 16599.0 25 16599.0 26 24726.5 27 32854.0 28 32854.0 29 32854.0 30 43989.5 31 55125.0 32 55125.0 33 55125.0 34 63632.0 35 72139.0 36 72139.0 37 72139.0 38 78525.5 39 84912.0 40 84912.0 41 84912.0 42 89167.0 43 93422.0 44 93422.0 45 93422.0 46 95299.0 47 97176.0 48 97176.0 49 97176.0 50 92332.5 51 87489.0 52 87489.0 53 87489.0 54 76522.0 55 65555.0 56 65555.0 57 65555.0 58 55143.0 59 44731.0 60 44731.0 61 44731.0 62 36079.0 63 27427.0 64 27427.0 65 27427.0 66 21010.0 67 14593.0 68 14593.0 69 14593.0 70 10479.5 71 6366.0 72 6366.0 73 6366.0 74 4381.5 75 2397.0 76 2397.0 77 2397.0 78 1636.0 79 875.0 80 875.0 81 875.0 82 561.5 83 248.0 84 248.0 85 248.0 86 159.5 87 71.0 88 71.0 89 71.0 90 42.0 91 13.0 92 13.0 93 13.0 94 7.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08514106389920865 2 0.03304817611877178 3 0.03388838398619818 4 0.030247483227350446 5 0.024646097444507773 6 0.3794938867875913 7 0.19422805202006976 8 0.20515075429661297 9 0.2097718975674582 10 0.014003464457106688 11 0.001400346445710669 12 0.0026606582468502705 13 0.0 14 0.08192026707407413 15 0.001260311801139602 16 0.0026606582468502705 17 0.004621143270845207 18 0.003500866114276672 19 0.002380588957708137 20 0.0015403810902817358 21 2.8006928914213377E-4 22 0.0 23 0.0 24 1.4003464457106689E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 714109.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.01294819340542 #Duplication Level Percentage of deduplicated Percentage of total 1 92.81191061925529 78.90214149205724 2 5.507248921323847 9.363749344733844 3 0.8440828886684149 2.1527392465592388 4 0.2890228112512079 0.9828272511844532 5 0.155013392013112 0.658907273224737 6 0.09859441072998448 0.5029080918928505 7 0.055717814487386795 0.33157149735261915 8 0.035615175331378035 0.24222008442763934 9 0.03032490142204049 0.23202083462058493 >10 0.1457267100201775 2.30779913126836 >50 0.013206460608051638 0.7701780480344678 >100 0.0112248884529699 1.8626329965541935 >500 0.0014856469946577056 0.840170551044741 >1k 8.253594414765029E-4 0.8501341570451046 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 1326 0.18568593870123468 No Hit GTCCTACAGTGGACATTTCTAAATT 1306 0.18288524580981333 No Hit GTATCAACGCAGAGTACTTTTTTTT 1196 0.167481434906996 No Hit GTCCTACAGTGTGCATTTCTCATTT 1178 0.1649608113047168 No Hit CTGTAGGACCTGGAATATGGCGAGA 1052 0.14731644608876235 No Hit GTCCTAAAGTGTGTATTTCTCATTT 935 0.13093239267394755 No Hit CTTTAGGACGTGAAATATGGCGAGG 921 0.12897190764995262 No Hit GGTATCAACGCAGAGTACTTTTTTT 728 0.1019452212477367 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.4003464457106689E-4 0.0 0.0 0.0 0.0 7 1.4003464457106689E-4 0.0 0.0 0.0 0.0 8 1.4003464457106689E-4 0.0 0.0 0.0 0.0 9 1.4003464457106689E-4 0.0 0.0 0.0 0.0 10 1.4003464457106689E-4 0.0 0.0 0.0 0.0 11 2.8006928914213377E-4 0.0 0.0 0.0 0.0 12 2.8006928914213377E-4 0.0 0.0 0.0 0.0 13 2.8006928914213377E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATGTC 35 0.0021076442 16.35541 4 TAGGACC 330 0.0 14.166428 4 GAACTAC 65 5.2547817E-5 13.203621 1 TGTAGGA 810 0.0 13.192948 2 TCCAACG 95 7.649032E-8 12.962394 18 GTAGGAC 765 0.0 12.845588 3 GGGCTAC 75 1.4196856E-5 12.714598 1 GTGCTAG 75 1.4196856E-5 12.714598 1 TGCCGGT 60 4.1611813E-4 12.639758 10 GCCGGTT 60 4.1636417E-4 12.638873 11 GTCCTAG 130 1.8553692E-10 12.470086 1 GTTCTAA 115 5.013135E-9 12.438193 1 TAGGCAG 70 1.04671424E-4 12.266558 5 GTCCTAC 810 0.0 12.125959 1 CTGTAGG 880 0.0 11.811572 1 CCAACGA 65 8.211231E-4 11.658484 19 TAGAACT 100 1.8281426E-6 11.448788 4 TAGCCCT 160 4.1836756E-11 11.329529 4 TCCTACA 895 0.0 11.300344 2 GGACCTG 555 0.0 11.173741 6 >>END_MODULE