FastQCFastQC Report
Thu 26 May 2016
SRR1926577_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926577_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720604
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATA450.001056068218.1304862
GGACCTA350.00123261817.7189796
TGTAGGA4350.015.6297292
GTGTTAT604.6102738E-415.5380531
TATGGTC400.003003836815.5084995
CTAGCAC505.3800346E-415.2265833
TTAGGCT506.2790734E-414.9197974
GTATAAT902.9512212E-614.7981461
GTAGGAC3900.014.22263053
GTCCTAC3600.014.0582391
GTGTAGA1151.7389902E-713.8973891
CTGTAGG4150.013.7997171
TAGGACC2300.013.4370854
GTCATAC700.001480827313.3183311
GTATTTA1101.5997266E-613.3183311
GCTAAAC500.00943066913.0513563
GTGTTAG1051.4516265E-512.6841251
TCACGTC708.8595174E-512.48650558
CTCTACA1203.362502E-612.4647082
TTATACT953.7696336E-612.3396824
Produced by FastQC (version 0.11.5)