##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926577_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 720604 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.295120482262103 28.0 16.0 31.0 2.0 31.0 2 17.20321147259799 25.0 2.0 31.0 2.0 31.0 3 20.883804419625758 26.0 15.0 31.0 2.0 31.0 4 22.959565864191706 28.0 10.0 35.0 2.0 37.0 5 23.876203851213702 30.0 10.0 35.0 2.0 37.0 6 24.407442922881362 31.0 12.0 35.0 2.0 37.0 7 24.37179366198356 31.0 12.0 35.0 2.0 37.0 8 24.74882737259299 32.0 17.0 35.0 2.0 37.0 9 27.62401263384605 33.0 20.0 38.0 2.0 39.0 10 28.43126321807817 33.0 27.0 38.0 2.0 39.0 11 25.45819062897236 31.0 11.0 37.0 2.0 39.0 12 26.6103324433392 32.0 18.0 37.0 2.0 39.0 13 23.157184806079343 30.0 2.0 37.0 2.0 39.0 14 26.6792399154043 31.0 17.0 38.0 2.0 40.0 15 27.846521806706598 32.0 24.0 39.0 2.0 40.0 16 28.23629344272305 33.0 25.0 39.0 2.0 40.0 17 27.50154314991313 32.0 20.0 39.0 2.0 40.0 18 27.610118733728928 31.0 24.0 39.0 2.0 40.0 19 28.05744347797126 32.0 26.0 39.0 2.0 40.0 20 28.32561018256907 32.0 27.0 39.0 2.0 40.0 21 26.018939667279117 31.0 11.0 38.0 2.0 40.0 22 27.064251655555616 31.0 18.0 39.0 2.0 40.0 23 27.08143307558659 31.0 19.0 39.0 2.0 40.0 24 26.10733912107066 32.0 2.0 39.0 2.0 40.0 25 25.92860850064668 32.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.737760749520302 1101 2 10.722450061534026 1101 3 6.9844012140247 1101 4 8.326268127013392 1101 5 8.1865276616048 1101 6 7.778989571159009 1101 7 7.327106691692848 1101 8 5.726059887129043 1101 9 5.590199796240505 1101 10 5.250383769672958 1101 11 8.076639023141698 1101 12 5.3647330133191815 1101 13 10.432358816903111 1101 14 7.593371555646822 1101 15 6.716556590731518 1101 16 6.1946550329754935 1101 17 1.8212373211935144 1101 18 4.313961749714359 1101 19 5.24541393931926 1101 20 3.16643249152391 1101 21 0.2878202468285629 1101 22 4.310824743295008 1101 23 5.485458228524479 1101 24 7.065623151868465 1101 25 7.411902398760741 1102 1 5.848704505943111 1102 2 11.227801919064152 1102 3 7.0330663608142885 1102 4 8.24384323455731 1102 5 8.224205559709741 1102 6 6.751967277205203 1102 7 6.923785884301985 1102 8 7.093604139543249 1102 9 5.975428335888388 1102 10 5.238569013351508 1102 11 8.334798954067264 1102 12 5.518953494710523 1102 13 10.214228445320103 1102 14 7.513089202343398 1102 15 5.749423397924804 1102 16 6.071184478776313 1102 17 6.879051238460708 1102 18 6.793960441097628 1102 19 5.416371885252861 1102 20 5.869259745135118 1102 21 4.696803387483051 1102 22 5.94409003711154 1102 23 6.4561907588119 1102 24 7.913550341460088 1102 25 7.764230774318346 1103 1 5.417892344906136 1103 2 9.93205874571898 1103 3 6.751555116771822 1103 4 8.370744035896081 1103 5 7.907859162597315 1103 6 7.238719717329836 1103 7 7.404439802924262 1103 8 7.081881039560368 1103 9 5.968508767769819 1103 10 5.18952784441025 1103 11 6.05000761515436 1103 12 4.927275525014235 1103 13 9.503535540622206 1103 14 7.351932890479343 1103 15 6.383167731918935 1103 16 6.040183149690055 1103 17 3.9550676075661713 1103 18 2.8456945611081856 1103 19 4.846963100190454 1103 20 5.216377660113146 1103 21 7.489273626248043 1103 22 3.099231023226711 1103 23 5.499832386966432 1103 24 6.857333197815805 1103 25 2.4838078943950457 1104 1 5.875038873368037 1104 2 10.77442445100069 1104 3 7.185473691736355 1104 4 7.720931134102198 1104 5 7.70262093200251 1104 6 7.078029420347935 1104 7 7.247818551215847 1104 8 7.141062609835441 1104 9 6.038487909856727 1104 10 4.8472205464769615 1104 11 8.041314654797379 1104 12 4.754303460600013 1104 13 9.590689687998221 1104 14 7.2389179161331505 1104 15 6.452510233908026 1104 16 5.7602490591378945 1104 17 6.382608100082894 1104 18 3.910807073130364 1104 19 4.687483782232537 1104 20 3.574992289726193 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1.5429713603124782 2204 6 4.23690226411205 2204 7 5.654280927559455 2204 8 5.757398177186236 2204 9 4.182230876879075 2204 10 3.65276050945511 2204 11 5.100511766152746 2204 12 5.370901418393011 2204 13 2.8737906991804927 2204 14 4.781662755135073 2204 15 4.900635251857626 2204 16 4.728553338060241 2204 17 -1.1921757867941913 2204 18 2.992031927379653 2204 19 3.7677217351171315 2204 20 4.366656889469809 2204 21 5.996798449258865 2204 22 5.254491720497295 2204 23 4.2878103454550995 2204 24 6.085777082767102 2204 25 5.924974086796826 2205 1 6.293062326208194 2205 2 11.232744079423181 2205 3 7.661584786235455 2205 4 8.275728351257754 2205 5 8.585157387065351 2205 6 7.730467897757261 2205 7 7.833273653927925 2205 8 7.6158019673492205 2205 9 6.248900651935287 2205 10 5.655769171500637 2205 11 8.153004694566537 2205 12 7.239309042236432 2205 13 10.67548321728686 2205 14 7.949496363042815 2205 15 6.718131340467448 2205 16 6.396792331770936 2205 17 6.9968945975063725 2205 18 6.856102052647753 2205 19 5.693902664111153 2205 20 5.977789586619416 2205 21 -0.2750897937757557 2205 22 4.716390635669345 2205 23 5.887650522426078 2205 24 7.82803460247947 2205 25 8.288910810347023 2206 1 4.551685280053473 2206 2 9.45469774014127 2206 3 6.558091593737192 2206 4 2.0133342969965007 2206 5 5.982476977621452 2206 6 6.544351226121613 2206 7 6.545773831391475 2206 8 6.453115808086331 2206 9 4.990272374183533 2206 10 4.111234693882338 2206 11 3.5997549820574832 2206 12 4.892912617062162 2206 13 8.713371684995593 2206 14 0.6198312012670435 2206 15 2.6778897060991476 2206 16 3.8357768143582085 2206 17 4.827299782281418 2206 18 -0.9286346624309694 2206 19 1.2934313209250448 2206 20 2.9513652641752444 2206 21 3.9034846770279437 2206 22 -1.5882397432508277 2206 23 -2.0304660214358137 2206 24 2.1474831877400575 2206 25 3.7942258436391825 2207 1 1.7075299087372464 2207 2 -14.62731881365858 2207 3 -6.52155146278637 2207 4 -4.405176207766097 2207 5 1.4924408116950936 2207 6 2.172965651575769 2207 7 3.269166909586854 2207 8 3.4947291620859104 2207 9 0.8438489173035144 2207 10 0.44775465102415524 2207 11 -1.6428029163658486 2207 12 -2.197533406246311 2207 13 0.6655203155102036 2207 14 -5.951233283654574 2207 15 -1.335139389613996 2207 16 -0.061769242584919226 2207 17 1.068852108447448 2207 18 1.091806194316593 2207 19 0.6449793820777074 2207 20 0.29345054363764334 2207 21 -4.2612141887559005 2207 22 -4.401910671463369 2207 23 -1.2855554306794517 2207 24 0.5512325852635769 2207 25 1.0689915279657782 2208 1 4.377955171730125 2208 2 6.417330371316616 2208 3 6.0507028567416725 2208 4 7.823525533326766 2208 5 2.747792388781427 2208 6 5.670755128812445 2208 7 6.142583590678257 2208 8 6.192260409330238 2208 9 4.377120523653012 2208 10 3.9457041611818937 2208 11 6.107182114568406 2208 12 5.51604250215825 2208 13 8.373024770848499 2208 14 2.3772957960690384 2208 15 3.8003937976212576 2208 16 4.497865882355068 2208 17 5.297596851583723 2208 18 0.1605495875153018 2208 19 2.561229908716655 2208 20 3.6167616792073005 2208 21 6.409322661817988 2208 22 5.362222576083607 2208 23 1.490214496413035 2208 24 2.93358443778072 2208 25 4.367234649543985 2209 1 3.5031957117392025 2209 2 5.314910641182756 2209 3 5.732321592249445 2209 4 1.4439443165821224 2209 5 5.885749472464845 2209 6 6.341434015324847 2209 7 6.913395167046428 2209 8 5.035313279404324 2209 9 4.774428897530466 2209 10 3.9384624436296356 2209 11 -0.5449588284695288 2209 12 3.3628800029295327 2209 13 3.115075921894075 2209 14 4.36558668037398 2209 15 4.852696436598169 2209 16 4.21634829330841 2209 17 1.950770019053774 2209 18 4.1046474262449415 2209 19 4.558644157681119 2209 20 4.736601782452809 2209 21 7.166818543011836 2209 22 5.641728769141867 2209 23 0.7391858761165047 2209 24 -2.939092120171761 2209 25 0.3377418638701073 2210 1 -2.2108338077170764 2210 2 -14.62731881365858 2210 3 -8.099334793860736 2210 4 -2.494156961357856 2210 5 -0.6449919840038305 2210 6 -1.5075315473037847 2210 7 -1.47163061910204 2210 8 -22.072091114411787 2210 9 -13.858040709514956 2210 10 -7.6110928799237065 2210 11 -11.052999051271422 2210 12 -5.34508214348314 2210 13 -5.518242504133781 2210 14 -4.408040401663069 2210 15 -6.182176188204398 2210 16 -5.766851073704466 2210 17 -5.282355310003805 2210 18 -5.2403185910814365 2210 19 -11.19235797221885 2210 20 -7.614191545456059 2210 21 -5.514800300620003 2210 22 -9.803118937027659 2210 23 -8.075995195294976 2210 24 -11.242587160376615 2210 25 -8.627461044998892 2211 1 0.014347582138071857 2211 2 5.068179862422621 2211 3 0.7480405695679551 2211 4 1.6208811986546046 2211 5 1.3967390398267092 2211 6 0.07402898953230874 2211 7 -21.782292383807924 2211 8 -13.867325006733058 2211 9 -9.224990115049454 2211 10 -7.504953044245394 2211 11 -10.1306178470245 2211 12 -5.065607914471148 2211 13 -7.216159441208699 2211 14 -4.922193839958066 2211 15 -5.339302310939459 2211 16 -12.1447917634402 2211 17 -7.794182966927121 2211 18 -5.91580921981307 2211 19 -9.112627558429327 2211 20 -6.900047679613163 2211 21 -4.620814396461338 2211 22 -6.426829900892464 2211 23 -8.792153332203753 2211 24 -23.618506278023673 2211 25 -23.455586044998892 2212 1 -0.3299898656626432 2212 2 -14.62731881365858 2212 3 -7.80941399345102 2212 4 -20.508862843710798 2212 5 -21.23874198400383 2212 6 -21.731796253186136 2212 7 -9.947466513161158 2212 8 -9.462140865655568 2212 9 -4.793528710685575 2212 10 -5.018329673895028 2212 11 -5.160169998008325 2212 12 -2.2723895403224716 2212 13 -5.868349505889707 2212 14 -3.3108270200417635 2212 15 -3.2022760565099304 2212 16 -3.429158104725829 2212 17 -3.82144295998917 2212 18 -2.792945164098704 2212 19 -3.441308074647882 2212 20 -3.612077117595362 2212 21 -1.3196547050683485 2212 22 -2.805945795382943 2212 23 -3.034960479169719 2212 24 -1.9707450839938225 2212 25 -2.5391681345511294 2213 1 -19.852377925364134 2213 2 -14.62731881365858 2213 3 -8.902591559658976 2213 4 -5.886764422169481 2213 5 -21.23874198400383 2213 6 -11.037274433316126 2213 7 -21.782292383807924 2213 8 -11.122230390177803 2213 9 -8.647614826069656 2213 10 -7.475217934541654 2213 11 -6.634442091579469 2213 12 -5.266264178274774 2213 13 -20.423573386486723 2213 14 -13.123507350977611 2213 15 -7.412481297713384 2213 16 -6.663740069553501 2213 17 -5.071792881689859 2213 18 -5.261599108859578 2213 19 -6.259637042621385 2213 20 -6.09968365316265 2213 21 -4.156639696545501 2213 22 -5.063382844122533 2213 23 -4.814910312177382 2213 24 -3.8162778657674075 2213 25 -4.688640826800192 2214 1 -19.852377925364134 2214 2 -14.62731881365858 2214 3 -12.367661825042797 2214 4 -20.508862843710798 2214 5 -21.23874198400383 2214 6 -21.731796253186136 2214 7 -16.839314559098316 2214 8 -15.15445647872013 2214 9 -13.561636429746025 2214 10 -13.50381040119832 2214 11 -14.363855897969975 2214 12 -12.024158677739926 2214 13 -20.423573386486723 2214 14 -17.280044291656232 2214 15 -25.36048501173381 2214 16 -18.881267147684213 2214 17 -25.080149427650863 2214 18 -18.45041704440152 2214 19 -15.060342784940138 2214 20 -13.491831460978762 2214 21 -10.936880088184447 2214 22 -12.018238082626766 2214 23 -12.038885941614621 2214 24 -10.821251790167285 2214 25 -10.585469888715894 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 105902.0 3 8.0 4 428.0 5 1697.0 6 2550.0 7 2469.0 8 2151.0 9 1925.0 10 1864.0 11 1765.0 12 1738.0 13 1929.0 14 3148.0 15 7345.0 16 11084.0 17 11635.0 18 11813.0 19 13078.0 20 15496.0 21 17895.0 22 18846.0 23 20849.0 24 22450.0 25 23367.0 26 19745.0 27 15196.0 28 16209.0 29 19026.0 30 18270.0 31 21387.0 32 28559.0 33 36541.0 34 45376.0 35 52395.0 36 52634.0 37 54849.0 38 38985.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.27457043240625 18.34430840980604 11.650687853429003 25.7304333043587 2 13.968308533807349 20.230451743726867 42.024154789075304 23.777084933390483 3 18.839484563013514 24.302608916392767 29.60958496725756 27.248321553336158 4 11.313169830039085 17.30421533445251 38.780947702966685 32.60166713254172 5 12.439422031839396 37.242503676235536 37.40489738507768 12.913176906847387 6 31.299942736666754 37.34464067258388 18.47995132616674 12.875465264582628 7 27.386784927274004 31.987212205863923 22.596099478280003 18.029903388582063 8 24.240267607230887 38.48390716105969 20.64899527927476 16.626829952434665 9 26.047865926506297 15.682147798689716 20.262306833147182 38.007679441656805 10 13.538832589332939 30.08836326965254 35.56364200534552 20.809162135669002 11 33.38981824944979 22.695865628879343 24.77640977346498 19.13790634820589 12 22.55812991189258 25.156722439461248 32.55097728004918 19.73417036859699 13 27.99582453104554 21.675878395256774 25.5929447147397 24.735352358957986 14 19.873985415972392 22.34767824765735 28.573990499483177 29.204345836887086 15 22.25052264359642 31.605449516420524 23.775597373402917 22.368430466580143 16 21.423830093489297 28.810789189592168 28.107289756606686 21.658090960311853 17 20.998284739041427 28.557735912757735 29.182325516187685 21.26165383201315 18 20.744178695457684 28.161976273095068 30.6245563729191 20.469288658528153 19 22.379057567991108 27.4432860852553 27.626090869211357 22.551565477542233 20 22.421162660253064 28.220545518064004 28.561546502095414 20.796745319587515 21 21.704008069410907 26.75262976337588 27.14285506354079 24.400507103672425 22 21.91517308588619 28.546389277290512 28.36687710972699 21.171560527096307 23 21.632742649796345 28.147487511182977 28.44310711765327 21.77666272136741 24 21.863859400874016 27.706145874947772 28.790586470109268 21.63940825406894 25 22.07173916063515 27.69209396663101 28.62064654154453 21.61552033118931 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 785.0 1 785.0 2 1697.0 3 2609.0 4 2609.0 5 2609.0 6 3703.0 7 4797.0 8 4797.0 9 4797.0 10 5706.5 11 6616.0 12 6616.0 13 6616.0 14 7907.5 15 9199.0 16 9199.0 17 9199.0 18 13061.0 19 16923.0 20 16923.0 21 16923.0 22 23252.0 23 29581.0 24 29581.0 25 29581.0 26 38362.0 27 47143.0 28 47143.0 29 47143.0 30 56674.5 31 66206.0 32 66206.0 33 66206.0 34 73199.5 35 80193.0 36 80193.0 37 80193.0 38 84034.5 39 87876.0 40 87876.0 41 87876.0 42 88613.5 43 89351.0 44 89351.0 45 89351.0 46 87504.0 47 85657.0 48 85657.0 49 85657.0 50 78886.5 51 72116.0 52 72116.0 53 72116.0 54 62082.0 55 52048.0 56 52048.0 57 52048.0 58 43034.0 59 34020.0 60 34020.0 61 34020.0 62 26798.0 63 19576.0 64 19576.0 65 19576.0 66 14654.5 67 9733.0 68 9733.0 69 9733.0 70 6882.0 71 4031.0 72 4031.0 73 4031.0 74 2743.5 75 1456.0 76 1456.0 77 1456.0 78 989.0 79 522.0 80 522.0 81 522.0 82 317.5 83 113.0 84 113.0 85 113.0 86 77.5 87 42.0 88 42.0 89 42.0 90 25.5 91 9.0 92 9.0 93 9.0 94 5.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.470644070807266 2 36.21753417966039 3 5.973183607085168 4 14.112744308940833 5 13.17838924013744 6 14.696005018012666 7 9.190900966411512 8 6.989691980616261 9 0.10546708039366975 10 1.6096219282712834 11 5.986228219660174 12 6.096274791702516 13 16.114398476833323 14 1.7252193992817138 15 3.0187176313203925 16 3.204395201802932 17 2.9954038556544234 18 1.65930247403567 19 1.6168380969297977 20 0.2957241425248819 21 4.657898096596743 22 1.7449250906184255 23 0.10546708039366975 24 8.98995842376673 25 0.10740989503250052 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 720604.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.50586194231035 #Duplication Level Percentage of deduplicated Percentage of total 1 97.42926744342893 92.07636898149126 2 1.9475925044858666 3.6811781669763954 3 0.292554911667612 0.829444622778124 4 0.10670662573073547 0.40337606558554673 5 0.06585082220257503 0.311164435593209 6 0.03658719301827379 0.20746225273449861 7 0.022914425037070727 0.15158832423285779 8 0.016962098965839226 0.12824142264939256 9 0.010094144336679505 0.08585602299971885 >10 0.0621996172511829 1.0373093903443489 >50 0.005382935601333105 0.34903026243896956 >100 0.0037377675738864318 0.65886500716766 >500 1.495106999411816E-4 0.08011504500794894 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 1.3877247420219705E-4 10 0.0 0.0 0.0 0.0 1.3877247420219705E-4 11 0.0 0.0 0.0 0.0 1.3877247420219705E-4 12 0.0 0.0 0.0 0.0 1.3877247420219705E-4 13 0.0 0.0 0.0 0.0 1.3877247420219705E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCTATA 45 0.0010560682 18.130486 2 GGACCTA 35 0.001232618 17.718979 6 TGTAGGA 435 0.0 15.629729 2 GTGTTAT 60 4.6102738E-4 15.538053 1 TATGGTC 40 0.0030038368 15.508499 5 CTAGCAC 50 5.3800346E-4 15.226583 3 TTAGGCT 50 6.2790734E-4 14.919797 4 GTATAAT 90 2.9512212E-6 14.798146 1 GTAGGAC 390 0.0 14.2226305 3 GTCCTAC 360 0.0 14.058239 1 GTGTAGA 115 1.7389902E-7 13.897389 1 CTGTAGG 415 0.0 13.799717 1 TAGGACC 230 0.0 13.437085 4 GTCATAC 70 0.0014808273 13.318331 1 GTATTTA 110 1.5997266E-6 13.318331 1 GCTAAAC 50 0.009430669 13.051356 3 GTGTTAG 105 1.4516265E-5 12.684125 1 TCACGTC 70 8.8595174E-5 12.4865055 8 CTCTACA 120 3.362502E-6 12.464708 2 TTATACT 95 3.7696336E-6 12.339682 4 >>END_MODULE