FastQCFastQC Report
Thu 26 May 2016
SRR1926577_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926577_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720604
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11410.15833939306470682No Hit
CTGTAGGACGTGGAATATGGCAAGA10560.14654373275752008No Hit
GTCCTACAGTGGACATTTCTAAATT10260.14238055853145415No Hit
GTCCTACAGTGTGCATTTCTCATTT8710.12087082503011363No Hit
GTCCTAAAGTGTGTATTTCTCATTT8040.11157306925856643No Hit
CTTTAGGACGTGAAATATGGCGAGG7430.10310794833223241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAGT551.9042024E-413.86354
GTCTAAG852.6001726E-713.4501181
GGCGAGG2550.013.38012419
TAGGACC2700.013.06121354
GATATAC1650.012.702891
GTCCTAA3250.012.3120321
GGACCTG4100.012.0882956
AGGACCT4850.011.3980845
TCCAACG752.1167986E-411.37310518
TCTAGAC600.00572609711.1204593
GTCTAAT600.005746958311.1150281
TTAGGAC3950.011.1003483
CCAACGA600.005974584311.05718519
ATAATAC951.3078634E-511.0368453
AGGACGT5200.010.9974885
CGTGAAA3750.010.8971229
CTTACAC700.001448716910.893513
GAGGTGT1053.3241085E-610.892756
ATCCTAT700.001454633810.8881911
GTCCTAC6200.010.7564791
Produced by FastQC (version 0.11.5)