FastQCFastQC Report
Thu 26 May 2016
SRR1926576_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926576_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561530
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15260.2717575196338575No Hit
CTGTAGGACGTGGAATATGGCAAGA10700.19055081651915304No Hit
GTCCTACAGTGTGCATTTCTCATTT9010.16045447260164195No Hit
GTCCTAAAGTGTGTATTTCTCATTT8000.14246790020123593No Hit
GTACATGGGAAGCAGTGGTATCAAC7270.12946770430787313No Hit
CCCATGTACTCTGCGTTGATACCAC6450.11486474453724645No Hit
CTGTAGGACCTGGAATATGGCGAGA6380.11361815041048563No Hit
CTTTAGGACGTGAAATATGGCGAGG6060.1079194344024362No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAC350.00550621719.296441
CTAGACC300.00828121717.9803984
GTGTAAC807.4498894E-716.8843861
TAGGACC3200.016.8566234
TGTAGGA7350.016.762022
TTGTAGA1259.276846E-1116.2090111
TAGGCCC400.002260347416.1823584
CTGTAGG7350.015.9884791
CCTAGAC504.7495938E-415.4767813
CTAAGCT505.7176745E-415.1035334
GTAGGAC7700.014.7876483
GGGCTGA507.1036443E-414.67762856
AGGACCT5350.014.5121755
GTGTAGA954.458365E-614.21843051
GGACCTG5100.014.1842636
TGTAGAA2550.014.1142212
ACCTACA903.357206E-513.8427932
CTCTAAA600.006381950313.8427932
GTCCTAC8800.013.8144961
TAAGACC652.7398832E-413.2778324
Produced by FastQC (version 0.11.5)