##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926576_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 561530 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.37926557797446 27.0 7.0 31.0 2.0 31.0 2 16.321250868163766 16.0 2.0 30.0 2.0 31.0 3 20.06582016989297 23.0 15.0 31.0 2.0 31.0 4 21.98959984328531 28.0 2.0 35.0 2.0 37.0 5 22.960182002742506 30.0 2.0 35.0 2.0 37.0 6 23.59842038715652 31.0 2.0 35.0 2.0 37.0 7 23.414155966733745 30.0 7.0 35.0 2.0 37.0 8 23.657475112638682 31.0 10.0 35.0 2.0 37.0 9 26.519571527790145 32.0 17.0 37.0 2.0 39.0 10 27.26409808914929 32.0 24.0 37.0 2.0 39.0 11 24.24621480597653 30.0 8.0 37.0 2.0 39.0 12 25.47018146848788 31.0 16.0 37.0 2.0 39.0 13 21.996539810873863 28.0 2.0 37.0 2.0 39.0 14 25.49801257279219 31.0 17.0 38.0 2.0 40.0 15 26.66574893594287 31.0 17.0 38.0 2.0 40.0 16 27.04413299378484 32.0 18.0 39.0 2.0 40.0 17 26.413619931259237 31.0 17.0 38.0 2.0 40.0 18 26.531410610296867 31.0 17.0 38.0 2.0 40.0 19 26.936259861449965 31.0 18.0 39.0 2.0 40.0 20 27.195522946236178 31.0 22.0 39.0 2.0 40.0 21 25.00103289227646 30.0 8.0 38.0 2.0 40.0 22 25.963863017113958 31.0 10.0 38.0 2.0 40.0 23 25.983471942727903 31.0 2.0 39.0 2.0 40.0 24 24.970827916585044 31.0 2.0 39.0 2.0 40.0 25 24.789029971684506 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.702499074866623 1101 2 10.748894602242935 1101 3 6.954883406184251 1101 4 8.458318973392025 1101 5 8.232613064574089 1101 6 7.724364142710716 1101 7 7.241951902276739 1101 8 5.756775990174461 1101 9 5.818472501306868 1101 10 5.176703985663931 1101 11 8.358247660302574 1101 12 5.726427327613596 1101 13 10.28250052762878 1101 14 7.64879684410398 1101 15 6.693419110828444 1101 16 6.269194036866196 1101 17 2.6902987181372353 1101 18 4.836464083695962 1101 19 5.379550503600104 1101 20 3.081652581130374 1101 21 0.24945317410356083 1101 22 4.506455456036733 1101 23 5.654535364690879 1101 24 7.062611151684116 1101 25 7.6436906891506595 1102 1 5.287003868698964 1102 2 10.409156971832854 1102 3 6.661767086495264 1102 4 7.759506397681399 1102 5 7.91158828313716 1102 6 6.611887501492209 1102 7 6.8330253263219625 1102 8 6.504475326268594 1102 9 5.4512536753027945 1102 10 4.585254943021834 1102 11 7.349427666124662 1102 12 4.861041989885525 1102 13 9.59609510369624 1102 14 6.886359173314897 1102 15 4.679819370005273 1102 16 5.440962073601696 1102 17 5.975466009269823 1102 18 6.136355623826116 1102 19 4.798653995914009 1102 20 5.069013015345469 1102 21 3.981902395164493 1102 22 5.165569841289948 1102 23 5.605843456454501 1102 24 7.110075256631955 1102 25 7.049959763532527 1103 1 4.972615726662664 1103 2 9.384845165674417 1103 3 6.386850294258011 1103 4 7.746685187330755 1103 5 7.19886500601687 1103 6 6.738582113035555 1103 7 6.725478553742324 1103 8 6.235866238348681 1103 9 5.404606469120225 1103 10 4.69730806351992 1103 11 5.7966750090352654 1103 12 4.196876090047091 1103 13 8.53492113501122 1103 14 6.636925128033791 1103 15 5.640653174474721 1103 16 5.271697258835069 1103 17 3.4795406466921435 1103 18 1.7816658285160258 1103 19 3.890397798798542 1103 20 4.6424073479920125 1103 21 6.926785383878517 1103 22 1.9998710734981522 1103 23 4.484274963514448 1103 24 5.907940122967286 1103 25 2.226057431822742 1104 1 5.635746383113929 1104 2 10.50576273411107 1104 3 7.049455500756348 1104 4 7.553224068297119 1104 5 7.503883460844484 1104 6 6.986352154698725 1104 7 7.061590596915433 1104 8 7.1304162388147105 1104 9 5.909770092604457 1104 10 4.754584438544388 1104 11 7.7832393352942475 1104 12 4.763085114271384 1104 13 9.192965063093315 1104 14 6.769315214622349 1104 15 6.279138946548279 1104 16 5.7401120077841625 1104 17 6.018775796614314 1104 18 3.6206521178839957 1104 19 4.350190974240572 1104 20 3.6200308695086605 1104 21 2.9269007265511107 1104 22 4.966689666270945 1104 23 5.282060617216132 1104 24 6.254308343381311 1104 25 5.42095509141506 1105 1 3.2596483932099716 1105 2 9.407387850288423 1105 3 6.210504650611469 1105 4 5.546950491128019 1105 5 2.090708948789377 1105 6 4.665647983248288 1105 7 5.643030922758744 1105 8 3.069514246942571 1105 9 3.5008075791643343 1105 10 2.9743714922866644 1105 11 6.402943014699421 1105 12 4.0443345447745465 1105 13 4.803887101254158 1105 14 3.8585758710471865 1105 15 4.037526495980604 1105 16 4.373041681758615 1105 17 5.161013326911547 1105 18 2.438276450433701 1105 19 2.214418372050062 1105 20 1.6419678877143369 1105 21 2.2652023503004983 1105 22 3.956429611234771 1105 23 1.5737865115539655 1105 24 4.553727001008923 1105 25 5.300342495056192 1106 1 6.473156443859057 1106 2 11.299784052716152 1106 3 7.802630612152061 1106 4 9.137971526873923 1106 5 8.72659818108782 1106 6 7.468915723630662 1106 7 7.444928599530233 1106 8 6.99922388220612 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7 4.990983198849019 2204 8 5.370660739305112 2204 9 3.603072600661065 2204 10 3.023783681923959 2204 11 4.508964429871803 2204 12 4.6034942497624876 2204 13 2.874417353971836 2204 14 4.121087668034146 2204 15 4.143773947248853 2204 16 3.650249320380496 2204 17 -1.3275601989020096 2204 18 2.5034039744063428 2204 19 3.0930591261251195 2204 20 3.2953776538718387 2204 21 5.217138221296803 2204 22 4.6399697559444775 2204 23 3.7583356627534705 2204 24 5.05378991827272 2204 25 5.144904229708459 2205 1 5.644966072889176 2205 2 10.35148131594076 2205 3 6.983770157293225 2205 4 7.692192096154034 2205 5 7.727094920167055 2205 6 7.1614866909443755 2205 7 7.166568438004386 2205 8 6.75256723596571 2205 9 5.432482976428457 2205 10 4.830597417335145 2205 11 7.239079791134703 2205 12 6.4026071149044945 2205 13 9.383096433780242 2205 14 6.8156906498866725 2205 15 5.61516300479456 2205 16 5.3958574968629875 2205 17 6.1592474648637605 2205 18 5.994900297687732 2205 19 4.356996925490968 2205 20 4.868368951180077 2205 21 -1.2409134551519578 2205 22 3.155752660889494 2205 23 4.641628518450698 2205 24 6.504704038221448 2205 25 6.84644575301683 2206 1 4.529156674624836 2206 2 9.268352898908624 2206 3 6.298139226339458 2206 4 1.9651258215746417 2206 5 6.07103320022215 2206 6 6.324555703374553 2206 7 6.514803622767065 2206 8 6.193665418336991 2206 9 4.951320488364303 2206 10 3.7607906724200255 2206 11 3.657324524574509 2206 12 4.885700463786119 2206 13 8.269815744923463 2206 14 0.4087437760221633 2206 15 2.701092648789455 2206 16 3.4629792044706633 2206 17 4.584440339691589 2206 18 -0.8047063475155376 2206 19 1.534236850791583 2206 20 3.1076710527340623 2206 21 3.9507318748689144 2206 22 -1.888397295592199 2206 23 -2.5966468890581105 2206 24 1.6272439863374863 2206 25 3.3474872348363185 2207 1 1.6903497876127318 2207 2 -14.132057778709445 2207 3 -6.230208698363919 2207 4 -4.429878603090071 2207 5 1.3317318676677878 2207 6 2.0656354933962895 2207 7 3.262333542951268 2207 8 3.5307711721194366 2207 9 1.1795730465078336 2207 10 0.6192314721244756 2207 11 -1.194981885604797 2207 12 -1.9208901387415267 2207 13 1.0888319228723091 2207 14 -4.736683784054474 2207 15 -1.1699455579755593 2207 16 -0.14599642121129364 2207 17 0.9331984265181177 2207 18 0.8615686862064216 2207 19 -0.0049837208922802745 2207 20 0.06853424897438387 2207 21 -4.033068697330446 2207 22 -4.189418405109095 2207 23 -1.3639761098875418 2207 24 0.7172236242882235 2207 25 1.1862833823709629 2208 1 4.661547294568955 2208 2 6.289151339229107 2208 3 6.304499845245687 2208 4 7.994959983521635 2208 5 3.6373267024393634 2208 6 6.059742919863524 2208 7 6.6213254194104145 2208 8 6.480166986360306 2208 9 4.692575273328373 2208 10 4.2470020391136245 2208 11 6.346465174535947 2208 12 5.800881697162119 2208 13 8.435420782134763 2208 14 2.9061012344608983 2208 15 4.176477430110744 2208 16 4.741215325785401 2208 17 5.462567991645361 2208 18 0.39617667041548543 2208 19 2.937954521122059 2208 20 4.145360594293294 2208 21 6.759341452326822 2208 22 5.793970633294229 2208 23 1.4454099179539348 2208 24 3.139042318799131 2208 25 4.751070590385861 2209 1 3.0210758751952476 2209 2 5.019141387088261 2209 3 5.197252351107942 2209 4 0.6990289932991232 2209 5 5.180198933692804 2209 6 5.68975580700749 2209 7 6.173161811588837 2209 8 4.666443573199711 2209 9 4.398843468539152 2209 10 3.4290817781877116 2209 11 -0.3447652635132705 2209 12 2.9370555072198776 2209 13 1.8973613359202446 2209 14 3.429226268693988 2209 15 4.109406408202599 2209 16 3.854723470935774 2209 17 1.7792723965269737 2209 18 3.403982331871145 2209 19 3.791192997359385 2209 20 3.9408177468648837 2209 21 6.440727228516302 2209 22 4.79288860925821 2209 23 0.7959784714624512 2209 24 -2.879863648454915 2209 25 0.4506234157914122 2210 1 -1.7709894053332675 2210 2 -14.132057778709445 2210 3 -7.44869455947425 2210 4 -1.8957950334065465 2210 5 0.3827554278037013 2210 6 -0.612942286877594 2210 7 -0.11314266758293101 2210 8 -21.432617949219477 2210 9 -12.869040209695775 2210 10 -6.415846640443739 2210 11 -9.596987820100692 2210 12 -3.876793277284861 2210 13 -4.654845124481973 2210 14 -2.9714637677673714 2210 15 -4.91517643233086 2210 16 -4.161955909384421 2210 17 -3.828869473849746 2210 18 -4.076846878272718 2210 19 -9.56988875959755 2210 20 -6.391283878383266 2210 21 -4.860420732750214 2210 22 -9.94818442309979 2210 23 -7.785519546840543 2210 24 -10.595746150968488 2210 25 -8.070289961071605 2211 1 0.011735064102612824 2211 2 5.04415843750677 2211 3 0.5863716476724932 2211 4 1.2147001597732086 2211 5 1.180712982674006 2211 6 0.1933930417396148 2211 7 -21.242896582571746 2211 8 -13.603428760030287 2211 9 -8.99148654865218 2211 10 -7.119881950922004 2211 11 -9.61411340205849 2211 12 -4.760121168934898 2211 13 -6.8775720124437605 2211 14 -4.542572057264923 2211 15 -4.848451721042391 2211 16 -11.713708736036578 2211 17 -7.598308980470463 2211 18 -5.7259683487364725 2211 19 -8.313645939596341 2211 20 -6.368264348786557 2211 21 -3.9388269241765386 2211 22 -5.810681551100274 2211 23 -8.012008451852939 2211 24 -22.818341229268263 2211 25 -22.646352600892634 2212 1 -0.7878950923696522 2212 2 -14.132057778709445 2212 3 -7.735335322455528 2212 4 -19.889083624010574 2212 5 -20.77496269300167 2212 6 -21.423904255557684 2212 7 -9.933422898361219 2212 8 -9.430512686061583 2212 9 -4.724175026603817 2212 10 -5.195813672116881 2212 11 -5.380998181574846 2212 12 -2.505668821850975 2212 13 -6.3256516710497355 2212 14 -3.601121132656104 2212 15 -3.68867931705946 2212 16 -3.6548182666197917 2212 17 -3.90234880977345 2212 18 -3.1935359163040395 2212 19 -3.6776857688993267 2212 20 -3.66729706571401 2212 21 -1.6222693139347193 2212 22 -2.8958024614843403 2212 23 -2.953231780444689 2212 24 -2.092025439794579 2212 25 -2.372668390366318 2213 1 -19.254210881843335 2213 2 -14.132057778709445 2213 3 -8.704207502906657 2213 4 -5.648823883750833 2213 5 -20.77496269300167 2213 6 -10.997497329150757 2213 7 -21.242896582571746 2213 8 -10.850366866968395 2213 9 -8.373085940251574 2213 10 -7.287581728621781 2213 11 -6.712098660043747 2213 12 -5.269243668057399 2213 13 -19.784599039470788 2213 14 -12.7299910346839 2213 15 -7.220000802591471 2213 16 -6.532754015081856 2213 17 -5.0629480351095175 2213 18 -5.056393059161181 2213 19 -5.805514431464832 2213 20 -5.925923176445384 2213 21 -4.134745960095575 2213 22 -5.188349738768462 2213 23 -5.039187579032067 2213 24 -3.871371532298568 2213 25 -4.7697292242692555 2214 1 -19.254210881843335 2214 2 -14.132057778709445 2214 3 -12.382053551720446 2214 4 -19.889083624010574 2214 5 -20.77496269300167 2214 6 -21.423904255557684 2214 7 -16.66703039380233 2214 8 -15.151853313616131 2214 9 -13.84526161404015 2214 10 -13.946649363495867 2214 11 -15.021971582819894 2214 12 -12.603333413437467 2214 13 -19.784599039470788 2214 14 -17.169109562512105 2214 15 -24.53710036969104 2214 16 -18.71276456606015 2214 17 -24.271822493983976 2214 18 -18.251050850593167 2214 19 -15.345677971628373 2214 20 -13.974422765267553 2214 21 -11.473556492454493 2214 22 -12.516721784882442 2214 23 -12.460024209546116 2214 24 -11.29384899509861 2214 25 -11.099160247248667 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 96928.0 3 4.0 4 373.0 5 1548.0 6 2308.0 7 2274.0 8 1997.0 9 1777.0 10 1634.0 11 1513.0 12 1533.0 13 1609.0 14 2566.0 15 5844.0 16 8957.0 17 9492.0 18 9833.0 19 11282.0 20 13363.0 21 14863.0 22 15253.0 23 16551.0 24 17321.0 25 17969.0 26 14729.0 27 11216.0 28 11900.0 29 14135.0 30 13720.0 31 15798.0 32 20709.0 33 25940.0 34 32443.0 35 37510.0 36 37284.0 37 39379.0 38 29975.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.414105213646934 18.760471103396746 12.66709539656475 25.158328286391573 2 12.837969489621656 19.449220048367316 44.924870199085376 22.78794026292565 3 20.55209060924478 23.486689721195003 28.175310845969232 27.785908823590987 4 11.410866835614407 16.52797770391247 38.62162043585174 33.43953502462138 5 11.575033244819435 38.3042849341565 37.46951689753926 12.651164923484806 6 31.213382376025084 38.460543118113975 17.953825202682925 12.372249303178018 7 28.158705548721883 30.607159666657434 21.454477856925322 19.779656927695356 8 23.121180613266436 41.43471723492828 19.660202618345426 15.783899533459858 9 26.119739617413757 15.720839343373742 19.889385540600536 38.27003549861197 10 14.212387361697273 30.486800248406272 35.178637060694776 20.122175329201678 11 33.54332057798846 21.967178784244293 25.365050355055494 19.12445028271175 12 24.236512271952947 24.44455018700572 33.16881905650143 18.15011848453991 13 29.48201059572887 21.926071888476926 25.1013335407538 23.490583975040405 14 18.9369379170293 24.362670438062082 27.53318102697998 29.167210617928635 15 21.82613800096056 34.63383575835755 22.869692748481405 20.67033349220048 16 19.683897989781297 28.537406571061254 31.918898708111232 19.85979673104622 17 19.93818529693134 29.814923835455147 30.48071969975716 19.766171167856356 18 19.47971957029764 28.075578340972086 33.88616148258184 18.558540606148437 19 22.795816523861603 26.756212563465564 27.44989751352604 22.99807339914679 20 22.45009858832943 28.976395953592043 29.625435788992398 18.948069669086127 21 20.532847755640905 27.34056198991839 27.149093855016805 24.977496399423906 22 21.11407490149074 30.902984925771253 28.122240335393883 19.86069983734412 23 20.357866848286353 29.8094597943585 29.29294910310423 20.539724254250917 24 22.217846425280204 27.78333503989414 30.50582462330952 19.492993911516134 25 21.352870275937324 28.552630640525482 29.811251453146287 20.283247630390903 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 640.0 1 640.0 2 1470.0 3 2300.0 4 2300.0 5 2300.0 6 3185.5 7 4071.0 8 4071.0 9 4071.0 10 4754.5 11 5438.0 12 5438.0 13 5438.0 14 6543.0 15 7648.0 16 7648.0 17 7648.0 18 10832.0 19 14016.0 20 14016.0 21 14016.0 22 19380.0 23 24744.0 24 24744.0 25 24744.0 26 32706.0 27 40668.0 28 40668.0 29 40668.0 30 49856.0 31 59044.0 32 59044.0 33 59044.0 34 63357.0 35 67670.0 36 67670.0 37 67670.0 38 68712.5 39 69755.0 40 69755.0 41 69755.0 42 69218.0 43 68681.0 44 68681.0 45 68681.0 46 67959.5 47 67238.0 48 67238.0 49 67238.0 50 59696.5 51 52155.0 52 52155.0 53 52155.0 54 42885.0 55 33615.0 56 33615.0 57 33615.0 58 27432.5 59 21250.0 60 21250.0 61 21250.0 62 16706.5 63 12163.0 64 12163.0 65 12163.0 66 9147.5 67 6132.0 68 6132.0 69 6132.0 70 4401.0 71 2670.0 72 2670.0 73 2670.0 74 1865.0 75 1060.0 76 1060.0 77 1060.0 78 723.0 79 386.0 80 386.0 81 386.0 82 253.0 83 120.0 84 120.0 85 120.0 86 87.5 87 55.0 88 55.0 89 55.0 90 32.5 91 10.0 92 10.0 93 10.0 94 5.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.43217637526045 2 37.84909087671184 3 6.430466760458034 4 14.759852545723293 5 14.559507061065306 6 16.0461595996652 7 9.948533471052304 8 7.854255338094136 9 0.11807027229177426 10 1.6399836161914767 11 6.457357576621018 12 6.43687781596709 13 17.577333357077983 14 1.7826296012679643 15 3.2231581571777106 16 3.313091019179741 17 3.2001852082702613 18 1.6953680123947072 19 1.649422114579809 20 0.289566007159012 21 5.036596441864193 22 1.79135576015529 23 0.11593325378875573 24 9.560664612754438 25 0.12074154542054744 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 561530.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.38010436259599 #Duplication Level Percentage of deduplicated Percentage of total 1 96.6573605859189 85.42587615996584 2 2.225547086424418 3.9338816752412296 3 0.45333260982164714 1.2019675010101554 4 0.19742800376764388 0.6979483030833344 5 0.11023779686532972 0.4871413995830249 6 0.06553534025065903 0.347521212647489 7 0.046878658803313836 0.2900198530190784 8 0.03241034913895667 0.2291544031463331 9 0.02996603216959992 0.2383560945433935 >10 0.15053252040148313 2.558668886741681 >50 0.01485498979752048 0.8631281406333975 >100 0.014218317132233963 2.4297708716727975 >500 0.001273282131236838 0.8096735509823221 >1k 4.24427377078946E-4 0.4868919477299301 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1526 0.2717575196338575 No Hit CTGTAGGACGTGGAATATGGCAAGA 1070 0.19055081651915304 No Hit GTCCTACAGTGTGCATTTCTCATTT 901 0.16045447260164195 No Hit GTCCTAAAGTGTGTATTTCTCATTT 800 0.14246790020123593 No Hit GTACATGGGAAGCAGTGGTATCAAC 727 0.12946770430787313 No Hit CCCATGTACTCTGCGTTGATACCAC 645 0.11486474453724645 No Hit CTGTAGGACCTGGAATATGGCGAGA 638 0.11361815041048563 No Hit CTTTAGGACGTGAAATATGGCGAGG 606 0.1079194344024362 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATTAC 35 0.005506217 19.29644 1 CTAGACC 30 0.008281217 17.980398 4 GTGTAAC 80 7.4498894E-7 16.884386 1 TAGGACC 320 0.0 16.856623 4 TGTAGGA 735 0.0 16.76202 2 TTGTAGA 125 9.276846E-11 16.209011 1 TAGGCCC 40 0.0022603474 16.182358 4 CTGTAGG 735 0.0 15.988479 1 CCTAGAC 50 4.7495938E-4 15.476781 3 CTAAGCT 50 5.7176745E-4 15.103533 4 GTAGGAC 770 0.0 14.787648 3 GGGCTGA 50 7.1036443E-4 14.6776285 6 AGGACCT 535 0.0 14.512175 5 GTGTAGA 95 4.458365E-6 14.2184305 1 GGACCTG 510 0.0 14.184263 6 TGTAGAA 255 0.0 14.114221 2 ACCTACA 90 3.357206E-5 13.842793 2 CTCTAAA 60 0.0063819503 13.842793 2 GTCCTAC 880 0.0 13.814496 1 TAAGACC 65 2.7398832E-4 13.277832 4 >>END_MODULE