FastQCFastQC Report
Thu 26 May 2016
SRR1926576_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926576_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561530
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA29320.5221448542375295No Hit
GTCCTACAGTGGACATTTCTAAATT28750.5119940163481915No Hit
GTCCTACAGTGTGCATTTCTCATTT18860.33586807472441366No Hit
CCCATGTACTCTGCGTTGATACCAC18060.3216212847042901No Hit
GTACATGGGAAGCAGTGGTATCAAC17940.3194842662012715No Hit
CTTTAGGACGTGAAATATGGCGAGG17540.31236087119120975No Hit
GTCCTAAAGTGTGTATTTCTCATTT16690.2972236567948284No Hit
CTGTAGGACCTGGAATATGGCGAGA16340.29099068616102436No Hit
GTATCAACGCAGAGTACTTTTTTTT12910.22990757394974445No Hit
GATATACACTGTTCTACAAATCCCG12480.222249924313928No Hit
CTGAAGGACCTGGAATATGGCGAGA10820.19268783502217157No Hit
GAGTACATGGGAAGCAGTGGTATCA8860.15778319947286876No Hit
GTGTATATCAATGAGTTACAATGAA8770.15618043559560488No Hit
GGTATCAACGCAGAGTACTTTTTTT8310.1479885313340338No Hit
GATATACACTGTTCTACAATGCCGG7520.13391982618916176No Hit
GTGTATATCAATGAGTTACAATGAG7350.13089238330988548No Hit
GTTCTACAGTGTGGTTTTTATCATT7090.12626217655334532No Hit
CATGTACTCTGCGTTGATACCACTG6940.12359090342457216No Hit
ACCTGGAATATGGCGAGAAAACTGA6860.1221662244225598No Hit
CTGTAGGACATGGAATATGGCAAGA6680.118960696668032No Hit
CTGTAGAACATATTAGATGAGTGAG6400.11397432016098874No Hit
GTCCTTCAGTGTGCATTTCTCATTT6400.11397432016098874No Hit
TATCAACGCAGAGTACTTTTTTTTT6350.113083895784731No Hit
GACCTGGAATATGGCGAGAAAACTG6340.11290581090947947No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6150.10952219827970011No Hit
ACAGTGGACATTTCTAAATTTTCCA5900.10507007639841148No Hit
TTGTAGAACAGTGTATATCAATGAG5780.10293305789539295No Hit
GAATATGGCAAGAAAACTGAAAATC5730.10204263351913521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1001.8189894E-1216.09777519
TCCAACG1300.015.2978918
TAGGACC5850.015.1644554
TAGTAGC456.5509847E-414.8370066
TGTAGGA13100.013.5511192
CTGTAGG12500.013.4356361
GTAGGAC12800.013.3419933
GGACCTG8950.013.3213356
GATATAC4550.013.00276851
TGTAGAA4800.012.9242582
AGGACCT9600.012.9173025
GTGTAGG1051.8635546E-812.723141
ATCCCGT1207.585186E-1012.64146410
TGTCGAG751.523135E-512.6268318
GTCCTAG1301.8189894E-1012.4784641
CCTAGGA550.002959798312.1469952
GTTACAA3600.012.10071115
GGCGAGG4700.012.0884419
CTCTGAC802.7263859E-511.9300842
TAGGACA3050.011.884574
Produced by FastQC (version 0.11.5)