##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926576_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 561530 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.287165423040623 31.0 28.0 31.0 22.0 34.0 2 28.748168397058038 31.0 28.0 33.0 23.0 34.0 3 29.066892240841984 31.0 28.0 33.0 24.0 34.0 4 32.7360372553559 35.0 33.0 37.0 26.0 37.0 5 32.42878742008441 35.0 33.0 37.0 25.0 37.0 6 32.52724342421598 35.0 33.0 37.0 25.0 37.0 7 32.31838904421848 35.0 33.0 37.0 23.0 37.0 8 32.28473456449344 35.0 33.0 37.0 23.0 37.0 9 33.48282371378199 37.0 33.0 39.0 19.0 39.0 10 33.38620376471426 37.0 33.0 39.0 19.0 39.0 11 33.623163499723965 37.0 33.0 39.0 23.0 39.0 12 33.4149377593361 37.0 33.0 39.0 19.0 39.0 13 33.57218492333446 37.0 33.0 39.0 23.0 39.0 14 34.16278204192118 38.0 33.0 40.0 17.0 41.0 15 34.325296956529485 38.0 33.0 40.0 19.0 41.0 16 34.30570583940306 38.0 33.0 40.0 19.0 41.0 17 34.23551190497391 38.0 33.0 40.0 18.0 41.0 18 34.206934625042294 38.0 33.0 40.0 18.0 41.0 19 34.229481951097895 39.0 34.0 40.0 18.0 41.0 20 34.23425284490588 39.0 34.0 40.0 18.0 41.0 21 34.130762381350955 39.0 34.0 40.0 18.0 41.0 22 34.078300357950596 39.0 34.0 40.0 18.0 41.0 23 33.70464089184905 39.0 33.0 40.0 10.0 41.0 24 33.80108987943654 39.0 34.0 40.0 2.0 41.0 25 33.58510320018521 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3447845231648117 1101 2 0.3680549140913385 1101 3 0.4724408680257959 1101 4 0.2572085723933455 1101 5 0.13733623408619167 1101 6 -0.20814469561836546 1101 7 0.006849236823427418 1101 8 0.0883244538036223 1101 9 0.28615261275260906 1101 10 0.16080443505497755 1101 11 0.08098265191816978 1101 12 0.329413771961093 1101 13 0.07988613880233686 1101 14 0.3700838302351741 1101 15 -0.04821982695499827 1101 16 0.2894224732768649 1101 17 0.022010350626338493 1101 18 0.3300789321788429 1101 19 0.46898889809457245 1101 20 0.3583968047938342 1101 21 0.4170957625736449 1101 22 0.27343285954987095 1101 23 0.6743236566781192 1101 24 0.40927986864939925 1101 25 0.5866777170841146 1102 1 0.46337435704748486 1102 2 0.43561226635585015 1102 3 0.40963509101177564 1102 4 0.30605072806847033 1102 5 0.4173622456740276 1102 6 0.025745961808084417 1102 7 0.2645188990484044 1102 8 0.5456893954418831 1102 9 0.7931719852817452 1102 10 0.7574018000905198 1102 11 0.5727589522627952 1102 12 0.657425059299932 1102 13 0.6474247101370025 1102 14 0.9449399213602447 1102 15 0.7408515499688733 1102 16 0.44900872939240344 1102 17 0.7014597876679716 1102 18 0.5264265108474646 1102 19 0.5203430031363183 1102 20 0.6915329383638067 1102 21 0.7859297485442909 1102 22 0.769852744798392 1102 23 1.008074865700479 1102 24 0.7296684460878708 1102 25 0.8538153155666137 1103 1 0.2114032733713458 1103 2 0.06926273934496407 1103 3 -0.05010465261132779 1103 4 -0.11344176963104502 1103 5 -0.04135085490623425 1103 6 -0.17623068087992522 1103 7 -0.001874863503722679 1103 8 -0.0573498464508333 1103 9 0.07039635882841822 1103 10 0.16704183633054726 1103 11 -0.06409682331398159 1103 12 -0.10025643060987477 1103 13 0.01930486257220565 1103 14 0.4099536296469566 1103 15 0.26784705781417983 1103 16 0.018958840217806028 1103 17 0.08916774793640769 1103 18 0.15517559124496927 1103 19 0.19060252619675566 1103 20 -0.07668465318838713 1103 21 0.02094229771788747 1103 22 -0.13938396738908665 1103 23 0.28448194622572487 1103 24 0.28975308500047703 1103 25 0.30865484936659016 1104 1 0.2859822143625017 1104 2 0.349115520151944 1104 3 0.2795365501214775 1104 4 0.03905172923649758 1104 5 0.07632779807775592 1104 6 -0.07554118801485998 1104 7 0.2788272588014493 1104 8 0.270864136343306 1104 9 0.6068458084458044 1104 10 0.6379661622167063 1104 11 0.3325782785137932 1104 12 0.5659966335439535 1104 13 0.5311432150594086 1104 14 0.7620668472181933 1104 15 0.6375249837898167 1104 16 0.45199601085040086 1104 17 0.27765748127347223 1104 18 0.2732468753467856 1104 19 0.2829943371000141 1104 20 0.1683229897200178 1104 21 0.2510812215590974 1104 22 0.2817717428887576 1104 23 0.47206757942204547 1104 24 0.32659866096818746 1104 25 0.5430824234888192 1105 1 0.607706924146914 1105 2 0.5908532600240122 1105 3 0.6551151589090445 1105 4 0.4072861067992335 1105 5 0.48137900723344274 1105 6 0.009840055948473037 1105 7 0.3000262091048782 1105 8 0.26334222210497416 1105 9 0.6184521540073717 1105 10 0.5356025225396337 1105 11 0.30174571969615727 1105 12 0.37740433860837896 1105 13 0.22818684307318904 1105 14 0.535199808783986 1105 15 0.4787879910975974 1105 16 0.5133353762942434 1105 17 0.4525362923463092 1105 18 0.4748259761796163 1105 19 0.43120702822315593 1105 20 0.499057139484016 1105 21 0.5434768441786062 1105 22 0.6029702375947181 1105 23 1.1444406044070092 1105 24 0.5683546247390794 1105 25 0.7432810639859682 1106 1 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0.6554481270149282 1107 14 1.1977712031032652 1107 15 1.012164567766746 1107 16 0.7432507734907077 1107 17 0.9745347338736181 1107 18 1.0015961699611395 1107 19 1.1521761927276515 1107 20 1.046695453935854 1107 21 0.8866362622346244 1107 22 0.9369179688181148 1107 23 0.993731156808515 1107 24 0.9701858301577673 1107 25 0.8358432102255051 1108 1 1.4193820812953035 1108 2 1.2690256997926639 1108 3 1.154446985660659 1108 4 0.9156255047623745 1108 5 -0.2460722735738088 1108 6 0.47325225074864363 1108 7 0.8578614980153318 1108 8 1.2011701909008536 1108 9 1.6111001203348465 1108 10 1.2571636646477415 1108 11 1.1365792509279373 1108 12 1.0792841610530388 1108 13 1.122249464099788 1108 14 1.4133220291619963 1108 15 1.2577396825973253 1108 16 1.3399188830547075 1108 17 1.4275564013819704 1108 18 1.370731393489983 1108 19 1.613109747305245 1108 20 1.641920666162008 1108 21 1.4584805255152915 1108 22 1.299115260980777 1108 23 1.5984494311032975 1108 24 1.406752455942346 1108 25 1.3813545970903363 1109 1 0.4789736673539551 1109 2 -0.5369101208736957 1109 3 0.4305194561043848 1109 4 0.671744036928807 1109 5 -0.6833730566230969 1109 6 -0.05789161536528553 1109 7 0.6216477716219657 1109 8 0.7738555893347581 1109 9 0.8712475178475145 1109 10 -1.270068025817487 1109 11 0.20287742381293583 1109 12 0.6603128728601959 1109 13 0.6107585996747957 1109 14 1.050143770295115 1109 15 0.7360292572940921 1109 16 0.8883635322179231 1109 17 0.9814609000768897 1109 18 0.7964092685091799 1109 19 1.0224387815444587 1109 20 0.9057161521131789 1109 21 1.0405256660035462 1109 22 1.011045247162265 1109 23 0.7950162973707577 1109 24 0.8634790028485355 1109 25 1.1870140474204405 1110 1 0.988777033721739 1110 2 -0.08876791543087847 1110 3 0.3788693066628248 1110 4 0.8728766466503757 1110 5 0.9839951988433171 1110 6 0.6696937236310987 1110 7 0.7947545830109846 1110 8 0.8514708166432357 1110 9 0.897870138580565 1110 10 0.7234886833220457 1110 11 0.48049265112141626 1110 12 0.9117113312525191 1110 13 0.8198603772428328 1110 14 0.9892376825178602 1110 15 1.038546167786457 1110 16 0.5749641299841599 1110 17 0.9553290768898535 1110 18 0.9631294195238098 1110 19 1.2906564943020484 1110 20 1.2857067136988363 1110 21 1.1259370235621446 1110 22 1.1794894818641666 1110 23 1.117674784078325 1110 24 1.236730536590862 1110 25 1.2245883749334183 1111 1 0.22267986819163 1111 2 0.19124699425552905 1111 3 0.3741112428037425 1111 4 0.22105448743447909 1111 5 0.4576954897044132 1111 6 0.26407896385629215 1111 7 0.3824895327824507 1111 8 0.6066321752985928 1111 9 0.6428513588935871 1111 10 0.7001362851437989 1111 11 0.4169289101751801 1111 12 0.5295569223034491 1111 13 0.28723423559266337 1111 14 0.5835373753620274 1111 15 0.5574052916183305 1111 16 0.5568117904669734 1111 17 0.391437404316342 1111 18 0.5608571181363118 1111 19 0.4620771511429851 1111 20 0.42307747604820634 1111 21 0.5290505408630821 1111 22 0.6230689472337687 1111 23 0.8419087276496455 1111 24 0.912336904574957 1111 25 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19 0.47024316259387433 2202 20 0.41288205464331895 2202 21 0.4376083625564391 2202 22 0.4293845237796816 2202 23 0.5468043768409601 2202 24 0.39336862757259183 2202 25 0.3402183265849885 2203 1 -0.2830469476899147 2203 2 -0.01840939679157927 2203 3 -0.183730480006016 2203 4 -0.7579177741283516 2203 5 -0.39374702374124837 2203 6 -0.2726015565868565 2203 7 -0.06956272330945978 2203 8 -0.012173885066808054 2203 9 0.3674976243301771 2203 10 0.24403709386903216 2203 11 0.0808568087690702 2203 12 -0.016126346834838046 2203 13 -0.03933766065402011 2203 14 0.33540145834349744 2203 15 0.4085908042742403 2203 16 0.17420048596185467 2203 17 0.2686880914901266 2203 18 0.23355723681993368 2203 19 0.4547686222463909 2203 20 0.11043546380923175 2203 21 0.31319965867754007 2203 22 0.32364427604036194 2203 23 0.35156114786909853 2203 24 0.279913111375663 2203 25 0.2867431555216413 2204 1 0.3863465130874566 2204 2 0.5137330064907424 2204 3 0.38819915345621325 2204 4 0.13168518867323797 2204 5 0.21310445493638497 2204 6 -0.12627328833712426 2204 7 0.15720052487963443 2204 8 0.39226668643438245 2204 9 0.7294568494994706 2204 10 0.5113863667844498 2204 11 0.31199890342622183 2204 12 0.4238605819816712 2204 13 0.23258289559745293 2204 14 0.8603329254023038 2204 15 0.729219673435324 2204 16 0.6764425570182553 2204 17 0.5510111312664634 2204 18 0.6892550020106469 2204 19 0.72698691101062 2204 20 0.6799069885991003 2204 21 0.7600380661716812 2204 22 0.6145761424964391 2204 23 1.0286774995565509 2204 24 0.755500860772031 2204 25 0.7641136691102304 2205 1 0.4279575230044763 2205 2 0.5903706641848672 2205 3 0.533928762847026 2205 4 0.2594933246780968 2205 5 0.3550156739878574 2205 6 0.30776840362806723 2205 7 0.12545593876346572 2205 8 0.2801233956025655 2205 9 0.809481136081132 2205 10 0.5790076310359495 2205 11 0.5675814440725162 2205 12 0.657362572132115 2205 13 0.44151833376785987 2205 14 0.8326192372150274 2205 15 0.8287643000291283 2205 16 0.7078127250004442 2205 17 0.8760667880161108 2205 18 0.8405941371384884 2205 19 0.6970098483377498 2205 20 0.7517750315053888 2205 21 0.87536897084685 2205 22 0.7191332496947069 2205 23 0.9549339928440119 2205 24 1.0599399081983236 2205 25 0.9383024298199345 2206 1 0.4274378383643267 2206 2 0.49721613616837246 2206 3 0.4998260427415673 2206 4 0.5859440053382201 2206 5 0.7712175820332519 2206 6 0.574011717441536 2206 7 0.6852006595280358 2206 8 0.7512999109269245 2206 9 0.938312225897846 2206 10 0.8224376341625046 2206 11 0.5733402258979154 2206 12 0.7363335120430534 2206 13 0.6068224126585235 2206 14 0.6468467661356883 2206 15 0.6960355754112868 2206 16 0.8176943383043707 2206 17 0.6633763426083519 2206 18 0.5304776094769039 2206 19 0.8142536607104631 2206 20 0.5506063748268133 2206 21 0.6700491746646335 2206 22 0.6517918380115191 2206 23 1.019328412360494 2206 24 0.8179485442585275 2206 25 0.6939450219900181 2207 1 -0.8993966791753856 2207 2 0.10382263312265394 2207 3 0.15498356939695057 2207 4 0.29432208649430436 2207 5 0.26710221427058656 2207 6 0.18583545254588074 2207 7 0.33305963730160926 2207 8 0.15731229998812069 2207 9 0.5784645546461391 2207 10 0.448456810573461 2207 11 0.03842618467550807 2207 12 0.0952898066655763 2207 13 0.10883069494354203 2207 14 -4.936691333234553 2207 15 -2.1281162206589173 2207 16 -0.8687537298784207 2207 17 -0.470916527237776 2207 18 -0.11293307673275166 2207 19 0.30311467468897035 2207 20 0.07448023322035624 2207 21 0.2747955941228426 2207 22 0.2836983959660415 2207 23 0.28572759444188733 2207 24 0.050103828511012694 2207 25 -0.0198221289554823 2208 1 0.36763621722562334 2208 2 0.6195565508754335 2208 3 0.6702053598933624 2208 4 0.7087646971407437 2208 5 0.8357314134030815 2208 6 0.6466566807390066 2208 7 0.711224148334118 2208 8 0.7450685191205295 2208 9 0.8108759898050266 2208 10 0.5897759425695952 2208 11 0.4811270235689804 2208 12 0.6409873156951917 2208 13 0.5268644165536571 2208 14 0.8178182893670538 2208 15 0.7106880969677931 2208 16 0.6894349154102173 2208 17 0.5440854839502336 2208 18 0.4957083968667817 2208 19 0.014766295457697254 2208 20 0.16420513587470964 2208 21 0.41782941940244456 2208 22 0.5214871673167707 2208 23 1.004808170512483 2208 24 0.9861615267996413 2208 25 0.7078008126868909 2209 1 -0.2531789488862515 2209 2 0.021821463843288313 2209 3 0.5296579818122531 2209 4 0.6236651085579084 2209 5 0.8654700224389629 2209 6 0.997980687514314 2209 7 1.1048680063750425 2209 8 1.0819810405259034 2209 9 1.4292625441837075 2209 10 0.9578863069543644 2209 11 0.9153824370478816 2209 12 0.9269506632936029 2209 13 1.060871698255049 2209 14 1.3098095546324302 2209 15 1.2894583099932149 2209 16 1.1518580911869307 2209 17 1.2431608546235609 2209 18 1.0901746758512303 2209 19 1.183693293455903 2209 20 1.0558404803396968 2209 21 1.2652330267693017 2209 22 1.2106227985646285 2209 23 1.304588768695055 2209 24 1.3384042722263558 2209 25 1.1275899678503762 2210 1 -0.26808191688283856 2210 2 -0.7221932046802699 2210 3 0.3386557493413491 2210 4 0.37072872114838873 2210 5 0.7466871739740455 2210 6 0.8619982486856514 2210 7 0.8598374119592549 2210 8 0.8023704748966267 2210 9 1.2057028611619174 2210 10 1.3855712789493353 2210 11 0.7602513697774853 2210 12 0.6877986756480112 2210 13 0.8640678407692732 2210 14 1.2775332041275007 2210 15 0.9728731740037802 2210 16 1.2089727216861732 2210 17 1.0721062279034257 2210 18 1.029728834848875 2210 19 1.257833722174297 2210 20 1.147292472884132 2210 21 1.2347400869293779 2210 22 1.1878478628910543 2210 23 1.4805643958047625 2210 24 1.3600338126919311 2210 25 1.401569201438683 2211 1 -1.242966734586446 2211 2 -0.1845331284967031 2211 3 0.4772554228403507 2211 4 0.6982721034568726 2211 5 0.9529124596624214 2211 6 0.6077216902480203 2211 7 0.9081619781361709 2211 8 0.8857890612682269 2211 9 1.1707624173624112 2211 10 1.1948245590751014 2211 11 0.8326487335842501 2211 12 0.9135076060549281 2211 13 0.8543344832506818 2211 14 1.2538582540096002 2211 15 0.869334870599701 2211 16 0.924482728337118 2211 17 0.7666306852466747 2211 18 0.838710030809942 2211 19 0.8315032306089094 2211 20 1.038855445252473 2211 21 1.0846282301061123 2211 22 1.1465684626854795 2211 23 1.6512325135869759 2211 24 1.2544699938395212 2211 25 1.4254340058404011 2212 1 0.7127658400934962 2212 2 0.00832604646773305 2212 3 0.18906196622584304 2212 4 0.10413270089642168 2212 5 0.5042922605159035 2212 6 0.4019464413148768 2212 7 0.4695560037407205 2212 8 0.4209810954076332 2212 9 0.6607481926113437 2212 10 0.17257704840653787 2212 11 0.3531608246226554 2212 12 0.48568399296815556 2212 13 0.28986791141568347 2212 14 0.29300476894558614 2212 15 0.3688227795612917 2212 16 -0.1966837012503646 2212 17 0.42108303233059985 2212 18 0.5485820080503387 2212 19 0.6073780527995183 2212 20 0.5942181764046808 2212 21 0.5804177037903173 2212 22 0.7038657599827758 2212 23 0.9108057710549744 2212 24 0.6238298800415123 2212 25 0.480455342966998 2213 1 0.10127802965831734 2213 2 0.12887742491384913 2213 3 -0.46045956487463613 2213 4 -1.0674667522819803 2213 5 -1.8053044585544988 2213 6 -0.6388806263542968 2213 7 -0.6197741397999472 2213 8 -0.2991214336422665 2213 9 -0.9584361318361374 2213 10 -0.7039141796636414 2213 11 -0.6971558762203642 2213 12 -0.8588979163505925 2213 13 -0.8432740343578402 2213 14 -7.585543875392531 2213 15 -3.6196483983835677 2213 16 -2.540664106809718 2213 17 -1.8427082640922734 2213 18 -5.501089898989989 2213 19 -3.5136950845894077 2213 20 -5.5322958358988075 2213 21 -2.7398306443527645 2213 22 -2.3802467941297785 2213 23 -5.011823529469066 2213 24 -2.6069538975843685 2213 25 -2.227175096768704 2214 1 0.08227851065879932 2214 2 0.015035472362217916 2214 3 -1.9151950921908067 2214 4 -2.2500646203637196 2214 5 -0.6135559442253395 2214 6 -0.7312598719593488 2214 7 -0.5130347024153501 2214 8 -0.6356472137164317 2214 9 -5.7078426393071595 2214 10 -1.6501507388034256 2214 11 -0.881177350564414 2214 12 -1.2714838370010355 2214 13 -1.4944633468374775 2214 14 -2.936662589414471 2214 15 -2.1285236330652566 2214 16 -1.4232107716144498 2214 17 -1.373833650056369 2214 18 -0.9675168412233859 2214 19 -4.664034888155019 2214 20 -2.43719350692551 2214 21 -3.7139380781376197 2214 22 -6.131092424007669 2214 23 -6.538030297611318 2214 24 -5.874681092730917 2214 25 -3.5625604402185616 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20403.0 3 16.0 4 338.0 5 2429.0 6 3070.0 7 2370.0 8 1809.0 9 1542.0 10 1309.0 11 1210.0 12 1154.0 13 1143.0 14 1176.0 15 1153.0 16 1161.0 17 1274.0 18 1454.0 19 1696.0 20 2094.0 21 2797.0 22 3547.0 23 4398.0 24 5296.0 25 6105.0 26 6822.0 27 7784.0 28 9009.0 29 10750.0 30 12951.0 31 16224.0 32 20960.0 33 27141.0 34 35029.0 35 45593.0 36 62104.0 37 90559.0 38 147660.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.229034943395014 19.450510077665854 9.326141324401553 29.99431365453758 2 11.93648316686351 20.631569946718965 43.15104486758123 24.280902018836297 3 19.850183929955197 23.976449840119 28.46451888733511 27.708847342590698 4 11.448248097035597 17.44385520191107 37.81595087653043 33.2919458245229 5 12.101974363907116 39.59896401114365 35.70759321405924 12.591468410889995 6 32.25858934040374 38.366465412898 17.1180722546918 12.256872992006466 7 28.361358414576017 31.03455663006357 20.591159915237444 20.012925040122965 8 23.53305556448129 42.53255215306563 18.6733502565256 15.261042025927473 9 25.912880478443274 15.891814692493082 19.390163349102917 38.805141479960724 10 14.950592744578381 30.86154569547747 33.68883759368499 20.499023966259156 11 34.31246315796972 22.54278127721413 24.82231233493435 18.322443229881802 12 24.615949023532806 24.782997396324934 32.02876508867099 18.572288491471273 13 30.603707727102737 22.651683792495504 24.993856071803823 21.750752408597936 14 19.374534411656725 24.898862627825164 26.767097116827475 28.959505843690632 15 22.140914700712532 34.71203899436704 22.199505981119007 20.947540323801416 16 19.84139422066612 29.062469947535075 31.525970672356006 19.570165159442794 17 19.90400883332443 31.15777101031148 29.073747573507152 19.86447258285694 18 19.72346192614966 28.39875764451109 33.03800083346096 18.839779595878287 19 23.55107565180225 27.2608277532412 27.061903405043452 22.126193189913092 20 23.14419890191497 29.096403887285117 28.831766463706742 18.927630747093176 21 20.9393977169519 27.680622584723878 27.32338432496928 24.05659537335494 22 21.830000178084873 31.106797499688348 26.780759710077824 20.28244261214895 23 20.495254038074545 30.295264723167058 28.280412444571084 20.929068794187312 24 23.275461375855482 27.605974423313572 29.623686839635493 19.494877361195453 25 21.51122825138461 29.293893469627623 28.954641782273434 20.240236496714335 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 263.0 1 263.0 2 263.5 3 264.0 4 264.0 5 264.0 6 338.0 7 412.0 8 412.0 9 412.0 10 699.0 11 986.0 12 986.0 13 986.0 14 1494.0 15 2002.0 16 2002.0 17 2002.0 18 3668.5 19 5335.0 20 5335.0 21 5335.0 22 9049.0 23 12763.0 24 12763.0 25 12763.0 26 19946.0 27 27129.0 28 27129.0 29 27129.0 30 38659.5 31 50190.0 32 50190.0 33 50190.0 34 56191.0 35 62192.0 36 62192.0 37 62192.0 38 65690.5 39 69189.0 40 69189.0 41 69189.0 42 71998.0 43 74807.0 44 74807.0 45 74807.0 46 78111.0 47 81415.0 48 81415.0 49 81415.0 50 74870.5 51 68326.0 52 68326.0 53 68326.0 54 56371.5 55 44417.0 56 44417.0 57 44417.0 58 36710.5 59 29004.0 60 29004.0 61 29004.0 62 23227.0 63 17450.0 64 17450.0 65 17450.0 66 13259.5 67 9069.0 68 9069.0 69 9069.0 70 6511.0 71 3953.0 72 3953.0 73 3953.0 74 2760.5 75 1568.0 76 1568.0 77 1568.0 78 1066.5 79 565.0 80 565.0 81 565.0 82 364.5 83 164.0 84 164.0 85 164.0 86 107.5 87 51.0 88 51.0 89 51.0 90 31.0 91 11.0 92 11.0 93 11.0 94 8.0 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0956315780100796 2 0.029740174167008 3 0.031164853169020356 4 0.030096343917511086 5 0.023863373283707013 6 0.3923209801791534 7 0.2452228732213773 8 0.24077075134008868 9 0.24575712784713194 10 0.013178280768614322 11 0.0019589336277669937 12 0.002849358004024718 13 0.0 14 0.0733709686036365 15 0.0023151033782700834 16 0.002137018503018539 17 0.003561697505030898 18 0.002849358004024718 19 0.001780848752515449 20 0.0012465941267608143 21 0.0 22 0.0 23 0.0 24 5.342546257546347E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 561530.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.35435971105805 #Duplication Level Percentage of deduplicated Percentage of total 1 92.76644949766141 70.83122854062141 2 5.185552709506376 7.918791137646029 3 0.86125917285507 1.9728267806587305 4 0.35397057165980433 1.0810878542256621 5 0.18813148860352344 0.7182329676905124 6 0.11814721663775195 0.5412633046811529 7 0.08770534632603304 0.46876798933725955 8 0.06372857911097758 0.38927638826513616 9 0.04858591082280026 0.3338771500668132 >10 0.2626345181540346 3.8811038352194673 >50 0.02933593886308509 1.6079089242257576 >100 0.026519677736790256 3.9969237754379896 >500 0.005397810512794091 2.6800265404809296 >1k 0.0025815615495971746 3.578684811443209 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 2932 0.5221448542375295 No Hit GTCCTACAGTGGACATTTCTAAATT 2875 0.5119940163481915 No Hit GTCCTACAGTGTGCATTTCTCATTT 1886 0.33586807472441366 No Hit CCCATGTACTCTGCGTTGATACCAC 1806 0.3216212847042901 No Hit GTACATGGGAAGCAGTGGTATCAAC 1794 0.3194842662012715 No Hit CTTTAGGACGTGAAATATGGCGAGG 1754 0.31236087119120975 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1669 0.2972236567948284 No Hit CTGTAGGACCTGGAATATGGCGAGA 1634 0.29099068616102436 No Hit GTATCAACGCAGAGTACTTTTTTTT 1291 0.22990757394974445 No Hit GATATACACTGTTCTACAAATCCCG 1248 0.222249924313928 No Hit CTGAAGGACCTGGAATATGGCGAGA 1082 0.19268783502217157 No Hit GAGTACATGGGAAGCAGTGGTATCA 886 0.15778319947286876 No Hit GTGTATATCAATGAGTTACAATGAA 877 0.15618043559560488 No Hit GGTATCAACGCAGAGTACTTTTTTT 831 0.1479885313340338 No Hit GATATACACTGTTCTACAATGCCGG 752 0.13391982618916176 No Hit GTGTATATCAATGAGTTACAATGAG 735 0.13089238330988548 No Hit GTTCTACAGTGTGGTTTTTATCATT 709 0.12626217655334532 No Hit CATGTACTCTGCGTTGATACCACTG 694 0.12359090342457216 No Hit ACCTGGAATATGGCGAGAAAACTGA 686 0.1221662244225598 No Hit CTGTAGGACATGGAATATGGCAAGA 668 0.118960696668032 No Hit CTGTAGAACATATTAGATGAGTGAG 640 0.11397432016098874 No Hit GTCCTTCAGTGTGCATTTCTCATTT 640 0.11397432016098874 No Hit TATCAACGCAGAGTACTTTTTTTTT 635 0.113083895784731 No Hit GACCTGGAATATGGCGAGAAAACTG 634 0.11290581090947947 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 615 0.10952219827970011 No Hit ACAGTGGACATTTCTAAATTTTCCA 590 0.10507007639841148 No Hit TTGTAGAACAGTGTATATCAATGAG 578 0.10293305789539295 No Hit GAATATGGCAAGAAAACTGAAAATC 573 0.10204263351913521 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 100 1.8189894E-12 16.097775 19 TCCAACG 130 0.0 15.29789 18 TAGGACC 585 0.0 15.164455 4 TAGTAGC 45 6.5509847E-4 14.837006 6 TGTAGGA 1310 0.0 13.551119 2 CTGTAGG 1250 0.0 13.435636 1 GTAGGAC 1280 0.0 13.341993 3 GGACCTG 895 0.0 13.321335 6 GATATAC 455 0.0 13.0027685 1 TGTAGAA 480 0.0 12.924258 2 AGGACCT 960 0.0 12.917302 5 GTGTAGG 105 1.8635546E-8 12.72314 1 ATCCCGT 120 7.585186E-10 12.641464 10 TGTCGAG 75 1.523135E-5 12.62683 18 GTCCTAG 130 1.8189894E-10 12.478464 1 CCTAGGA 55 0.0029597983 12.146995 2 GTTACAA 360 0.0 12.100711 15 GGCGAGG 470 0.0 12.08844 19 CTCTGAC 80 2.7263859E-5 11.930084 2 TAGGACA 305 0.0 11.88457 4 >>END_MODULE