FastQCFastQC Report
Thu 26 May 2016
SRR1926575_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926575_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668391
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC24420.36535500926852693No Hit
CCCATGTACTCTGCGTTGATACCAC23080.3453068637967896No Hit
GAGTACATGGGAAGCAGTGGTATCA11830.17699220964974094No Hit
GTCCTACAGTGGACATTTCTAAATT9020.1349509493694559No Hit
CATGTACTCTGCGTTGATACCACTG8830.13210830187719463No Hit
GCGTTGATACCACTGCTTCCCATGT8400.12567494176312966No Hit
ACGCAGAGTACATGGGAAGCAGTGG7140.10682370049866022No Hit
GTATCAACGCAGAGTACATGGGAAG6940.10383143998049046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA4500.017.1483732
GTCGAGG352.3610942E-416.96655519
CCTACAC802.0399966E-816.5509173
CTGTAGG4800.016.2234211
GTAGGAC5200.015.06557753
GATATAC3600.014.9180891
TCCTACA6050.014.8048972
GTCCTAC5700.014.6040241
TGTCGAG350.004592545314.55013518
TTTAGGA2600.014.3099392
ATGTCGA451.4710345E-414.29080117
TGTAGAA4050.014.2903112
GTTGGAC559.928904E-414.0432033
AATACCT450.00598669913.9794025
TAGGACC2400.013.9667974
TAGGACG5350.013.9457444
GGACGTG4500.013.7349426
GGACCTG3300.013.3328416
CTTTAGG2550.013.1630191
AGGACGT4950.013.1321645
Produced by FastQC (version 0.11.5)