##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926575_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 668391 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.11235339793624 27.0 2.0 30.0 2.0 31.0 2 15.532586465107999 12.0 2.0 30.0 2.0 31.0 3 19.045186126084882 19.0 10.0 30.0 2.0 31.0 4 20.938323526199486 27.0 2.0 35.0 2.0 37.0 5 21.540749351801566 28.0 2.0 35.0 2.0 37.0 6 21.79969209639268 29.0 2.0 35.0 2.0 36.0 7 21.864721398103804 29.0 2.0 35.0 2.0 37.0 8 22.083056175202838 29.0 2.0 35.0 2.0 37.0 9 24.69239113034137 31.0 11.0 37.0 2.0 39.0 10 25.334926712059257 31.0 10.0 37.0 2.0 39.0 11 22.55633603684071 29.0 2.0 37.0 2.0 39.0 12 23.609804740039888 30.0 2.0 37.0 2.0 39.0 13 20.431485462850336 26.0 2.0 35.0 2.0 39.0 14 23.622710359654754 30.0 2.0 37.0 2.0 40.0 15 24.648865708844074 30.0 2.0 38.0 2.0 40.0 16 24.99272431855007 31.0 2.0 38.0 2.0 40.0 17 24.335222945850557 30.0 2.0 38.0 2.0 40.0 18 24.415384109002066 30.0 2.0 37.0 2.0 40.0 19 24.768674323861333 30.0 2.0 38.0 2.0 40.0 20 25.01007344503442 31.0 2.0 38.0 2.0 40.0 21 22.956842626546436 30.0 2.0 37.0 2.0 40.0 22 23.8355633154845 30.0 2.0 37.0 2.0 40.0 23 23.802525767103386 30.0 2.0 37.0 2.0 40.0 24 22.905820096320866 30.0 2.0 38.0 2.0 40.0 25 22.707359913583517 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.993694652616515 1101 2 9.235273464019759 1101 3 6.209211736646498 1101 4 7.645567934990556 1101 5 7.225462030585209 1101 6 6.719131868975381 1101 7 6.609897623300746 1101 8 4.886564558121044 1101 9 5.081842920173539 1101 10 4.729317238849667 1101 11 7.17465274949053 1101 12 4.775718000528684 1101 13 9.053208542082512 1101 14 6.481653914474201 1101 15 5.901612678840063 1101 16 5.339492947698368 1101 17 1.4752781777205115 1101 18 3.8675297068471046 1101 19 4.188346156036669 1101 20 2.4593984579627772 1101 21 0.25459102461349303 1101 22 3.3928565904610544 1101 23 4.479077289802984 1101 24 5.663047700449848 1101 25 6.055474821596494 1102 1 5.927954819898872 1102 2 10.346269821206317 1102 3 6.965779906491569 1102 4 8.036649577151291 1102 5 8.213457504932762 1102 6 6.494821000593767 1102 7 6.594349713849077 1102 8 6.961150421631409 1102 9 6.129873050562686 1102 10 5.568695867730931 1102 11 8.187294747449016 1102 12 5.799203442505473 1102 13 9.911790723488686 1102 14 7.505566743544218 1102 15 5.498348499892366 1102 16 6.099670253203161 1102 17 6.837534845720928 1102 18 6.8033021669208935 1102 19 5.6066843499292816 1102 20 5.998900491860269 1102 21 5.011351638928314 1102 22 6.181494027485606 1102 23 6.15810557352707 1102 24 7.711190891964971 1102 25 7.475998863924655 1103 1 4.602112978413636 1103 2 8.603111023062052 1103 3 6.005049109545599 1103 4 7.39499593247762 1103 5 6.8644861749082295 1103 6 6.18185514385458 1103 7 6.271089870436857 1103 8 5.932171966620032 1103 9 5.296822017844423 1103 10 4.4032820112508375 1103 11 5.065471236611749 1103 12 4.146576508435892 1103 13 8.183587110796495 1103 14 6.308451553558065 1103 15 5.334864057463069 1103 16 5.181060273363634 1103 17 2.661753323726579 1103 18 1.6960103906130932 1103 19 3.553697927887164 1103 20 4.001183726280431 1103 21 6.076134772141277 1103 22 2.1605886683619815 1103 23 4.017369270909448 1103 24 5.223129293215081 1103 25 1.732631174120673 1104 1 5.43792994745964 1104 2 9.878803148746005 1104 3 6.857183666406147 1104 4 7.255979268535093 1104 5 7.232049472205109 1104 6 6.650810864933963 1104 7 6.619939116628899 1104 8 6.788391174129313 1104 9 5.743049086438223 1104 10 4.936107098228678 1104 11 7.725504237600223 1104 12 5.007370711469804 1104 13 8.986470805661881 1104 14 6.7455161778994075 1104 15 6.234889742194664 1104 16 5.64203426951871 1104 17 5.791970242959771 1104 18 3.667060584805231 1104 19 4.275288668930585 1104 20 3.7318754238855263 1104 21 2.6000277991258116 1104 22 4.908099973535506 1104 23 5.268081827629896 1104 24 6.301615606366443 1104 25 5.453791501328588 1105 1 3.4865950054020836 1105 2 9.286241312929427 1105 3 6.217392969248866 1105 4 5.618927543220536 1105 5 2.198230224410203 1105 6 4.626299674025631 1105 7 5.883981523151519 1105 8 3.2435259412055224 1105 9 4.116117448016244 1105 10 3.613641471481838 1105 11 6.862554045941206 1105 12 4.102148952132801 1105 13 5.150698698547533 1105 14 3.972920618683709 1105 15 4.528113087252208 1105 16 4.671677582079717 1105 17 5.482149740697359 1105 18 2.7209735496042278 1105 19 2.6475611030806263 1105 20 2.023296046993952 1105 21 2.9863761868360186 1105 22 4.339894437162467 1105 23 1.5391058519095182 1105 24 4.595412509917367 1105 25 5.26835267923984 1106 1 6.520674162423401 1106 2 11.129201959894523 1106 3 8.036121736948711 1106 4 9.130111892196876 1106 5 8.891774853577747 1106 6 7.811791060851835 1106 7 7.603016937876589 1106 8 7.464762750873923 1106 9 6.515024642195964 1106 10 6.056196262090019 1106 11 8.072703461207414 1106 12 5.568952156894849 1106 13 4.524272188611569 1106 14 5.910702087583086 1106 15 6.0038143168466505 1106 16 6.190398082335257 1106 17 6.509006909323162 1106 18 6.651739716215779 1106 19 6.5180403142161225 1106 20 6.243165722008232 1106 21 6.266774076382962 1106 22 6.67285598603058 1106 23 6.909709826295472 1106 24 7.26822389193342 1106 25 6.623613780162785 1107 1 3.7594540371918974 1107 2 -13.188039419415823 1107 3 -5.364490456954336 1107 4 1.5788147466520037 1107 5 4.399443813215573 1107 6 4.54645314157618 1107 7 4.439106981090276 1107 8 4.83199090150449 1107 9 3.2597361099914757 1107 10 2.7412860701271775 1107 11 5.195500886024419 1107 12 2.744931079198402 1107 13 3.909173120498565 1107 14 3.3971772323636387 1107 15 3.2618103188456544 1107 16 3.1068281026192324 1107 17 3.5454989574167612 1107 18 -0.9232058992118013 1107 19 1.407588187338387 1107 20 1.74886948777241 1107 21 -1.388284423778721 1107 22 1.7017621211982892 1107 23 -1.0987447699358235 1107 24 2.0190462748596048 1107 25 2.8576158967515504 1108 1 5.190801854550031 1108 2 10.25308686044766 1108 3 0.1486173236263646 1108 4 5.8178869892899705 1108 5 7.172094378115819 1108 6 7.243472830888319 1108 7 7.292292564568363 1108 8 7.0952499816612615 1108 9 5.38569664407898 1108 10 4.67284919842049 1108 11 7.10706264680233 1108 12 2.6690492499865286 1108 13 3.659075648656291 1108 14 4.96003420480093 1108 15 4.936378751352947 1108 16 4.923180240744106 1108 17 0.7106780873860075 1108 18 3.971184814485756 1108 19 4.874254272083608 1108 20 5.138366969507349 1108 21 4.9234028865512585 1108 22 1.9989181256092543 1108 23 4.731841166768579 1108 24 5.584420784954489 1108 25 5.972183863721689 1109 1 3.9080421024426073 1109 2 -13.188039419415823 1109 3 -5.089792226554712 1109 4 2.5308004630058107 1109 5 2.1647517411447446 1109 6 3.153303592995467 1109 7 4.232467475411056 1109 8 4.383993162622225 1109 9 3.8514205278111078 1109 10 3.0768828190810673 1109 11 1.291658134814007 1109 12 1.4017053557671098 1109 13 2.8356293469596174 1109 14 3.5661819958769208 1109 15 3.611003646710021 1109 16 3.588303711730802 1109 17 4.001380735886968 1109 18 4.205774322901654 1109 19 2.741783227126245 1109 20 1.8436250273959693 1109 21 1.143478076135107 1109 22 3.204017494225269 1109 23 3.521024384649717 1109 24 4.5357898057420165 1109 25 4.982880440633089 1110 1 4.002420789110058 1110 2 9.775728696526205 1110 3 7.072103745944215 1110 4 8.318211592346803 1110 5 9.044104938535266 1110 6 4.524341035864328 1110 7 7.192197347671691 1110 8 8.006117073712502 1110 9 6.644859724654733 1110 10 5.971038840800663 1110 11 3.384066529673184 1110 12 6.634050857544523 1110 13 10.773410119646051 1110 14 8.532247564844457 1110 15 7.904926260874639 1110 16 7.692665698527158 1110 17 8.234544140707467 1110 18 8.18692837188794 1110 19 7.196857155795335 1110 20 7.190791908914779 1110 21 5.410202362240035 1110 22 7.352497415315938 1110 23 7.721747557429904 1110 24 8.41099000887495 1110 25 8.788668752676529 1111 1 5.289326331313831 1111 2 9.230721049595903 1111 3 7.216239278680359 1111 4 7.9338850684228355 1111 5 8.275092302893288 1111 6 7.523799378301149 1111 7 7.275284886816692 1111 8 7.3141245021590855 1111 9 5.575363693775699 1111 10 5.091678269829867 1111 11 6.700226132032807 1111 12 6.046106607307834 1111 13 7.860931057669504 1111 14 5.598989790465314 1111 15 5.067942428670136 1111 16 4.858035118088736 1111 17 6.350868516208159 1111 18 6.1959362737159225 1111 19 5.004250098771081 1111 20 4.441237979849159 1111 21 7.201960063791269 1111 22 6.707956777343465 1111 23 6.48636320814726 1111 24 6.418545904294074 1111 25 6.97424336486792 1112 1 -1.6197149335691456 1112 2 7.358413283286879 1112 3 5.392278540303792 1112 4 -0.2608130845039618 1112 5 3.5514614798591992 1112 6 2.6123502407213586 1112 7 5.174522063692059 1112 8 5.304173282094798 1112 9 4.179598267545451 1112 10 3.0117997103658816 1112 11 -0.4099503132567044 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5.286502246841618 2204 5 0.8787824618732429 2204 6 3.4365302926594197 2204 7 4.4193042123887984 2204 8 4.499644331435956 2204 9 2.9729581309675908 2204 10 2.6966306712199746 2204 11 3.812199104844673 2204 12 3.9115310009247892 2204 13 2.5483211516745214 2204 14 3.7608340056098406 2204 15 3.495009555526245 2204 16 3.657958519198676 2204 17 -1.568462718852075 2204 18 2.0902014906666295 2204 19 3.0203105314206873 2204 20 3.37081021826544 2204 21 4.6860739904828534 2204 22 4.119978332330739 2204 23 3.09065840894365 2204 24 4.893003005935139 2204 25 4.834848287464297 2205 1 5.790995626905566 2205 2 9.885717012831176 2205 3 7.045917975193227 2205 4 7.2463522068594415 2205 5 7.457612967968235 2205 6 6.7740256382458135 2205 7 7.111949628478115 2205 8 6.723738323122106 2205 9 5.969324636281254 2205 10 5.22116935016005 2205 11 7.295374544190796 2205 12 6.638983800592726 2205 13 9.648624527879328 2205 14 7.245100561587936 2205 15 6.186475344823229 2205 16 5.610561328328039 2205 17 6.3869479988127225 2205 18 6.176778161830022 2205 19 4.912329661823623 2205 20 5.196928956171597 2205 21 -0.9310951493815125 2205 22 3.4632268383402725 2205 23 4.830180637202197 2205 24 6.671990083451728 2205 25 6.943306806719836 2206 1 3.924396134204912 2206 2 8.07342068245175 2206 3 5.798657595505002 2206 4 2.0092363579775707 2206 5 5.272336588839146 2206 6 5.618154856882512 2206 7 6.025976659204378 2206 8 5.918834526531086 2206 9 4.5482516195392115 2206 10 3.5350382502640123 2206 11 3.204200446161458 2206 12 4.322747309403486 2206 13 7.677909270749616 2206 14 0.0777019704939228 2206 15 2.1667585238602918 2206 16 3.150271626038787 2206 17 3.894438090167373 2206 18 -1.267967853598634 2206 19 0.5241728591490897 2206 20 1.9691961066461339 2206 21 2.8167345587903156 2206 22 -2.4674519584691588 2206 23 -2.971608597684387 2206 24 0.7558080497696125 2206 25 2.1264034294807423 2207 1 1.3629140643233484 2207 2 -13.188039419415823 2207 3 -5.943604498905083 2207 4 -4.044873333699172 2207 5 0.9746612702665729 2207 6 1.545975382572724 2207 7 2.6358516452015905 2207 8 2.986436361369446 2207 9 0.5054554550671639 2207 10 0.280919247640945 2207 11 -1.488216079022468 2207 12 -1.8382745960525853 2207 13 0.3541727588919805 2207 14 -5.101528654372412 2207 15 -1.396659931890163 2207 16 -0.558477608354103 2207 17 0.5091147602819994 2207 18 0.6675014690154697 2207 19 0.07733618042183821 2207 20 -0.04037961971679138 2207 21 -3.8580486343696307 2207 22 -3.943823977329643 2207 23 -1.1015894084059425 2207 24 0.3255703151228566 2207 25 0.7433416411680156 2208 1 5.201448153558051 2208 2 6.520641715809553 2208 3 6.34831591114709 2208 4 8.301004471783546 2208 5 3.9013957735017577 2208 6 6.379777935528018 2208 7 6.909609822569468 2208 8 6.8073739995067015 2208 9 5.391920042575908 2208 10 4.688030929063711 2208 11 6.589691544795006 2208 12 6.476431576617376 2208 13 8.943276563719508 2208 14 3.708772146877312 2208 15 5.06326685268224 2208 16 5.432272470683653 2208 17 6.264406713594646 2208 18 1.0445337407394106 2208 19 3.641881774120563 2208 20 4.8930462700480675 2208 21 7.340899054795258 2208 22 6.4048748680753675 2208 23 2.4820833223521177 2208 24 4.042023615610816 2208 25 5.162422714593735 2209 1 2.555670679316467 2209 2 4.193273711897309 2209 3 4.534975214250466 2209 4 0.30197129078041485 2209 5 4.187156834304552 2209 6 4.622542250913369 2209 7 4.946680585850579 2209 8 3.7460526614741774 2209 9 3.379781405228588 2209 10 2.5721953332615044 2209 11 -1.1835022743086654 2209 12 2.259242996323618 2209 13 1.0023663486022798 2209 14 2.739748296801711 2209 15 3.17939193207944 2209 16 2.659462287606356 2209 17 0.9378818657843233 2209 18 2.6480156943883095 2209 19 2.8697180107214066 2209 20 3.203107089708219 2209 21 5.234221539520082 2209 22 4.003106769729637 2209 23 -0.1313211733562163 2209 24 -3.318734775197658 2209 25 -0.5347282609378752 2210 1 -2.7700011493264967 2210 2 -13.188039419415823 2210 3 -7.79387211587021 2210 4 -2.770050569017336 2210 5 -1.5892932923153538 2210 6 -2.245172817141256 2210 7 -2.1404070419840764 2210 8 -19.707651042229525 2210 9 -13.000135477234984 2210 10 -7.853756316798805 2210 11 -10.038583934144144 2210 12 -5.408152520817229 2210 13 -5.562328420252081 2210 14 -4.704131645804889 2210 15 -6.52169648462867 2210 16 -6.089966064962912 2210 17 -5.511334405588144 2210 18 -5.766270060729365 2210 19 -10.051378923533424 2210 20 -7.522314929007518 2210 21 -5.917560857168937 2210 22 -9.851153146924165 2210 23 -8.30777158579984 2210 24 -10.336721661605598 2210 25 -8.30059147714065 2211 1 0.1218068896417499 2211 2 4.585594578849573 2211 3 0.5325142063688162 2211 4 1.5717491503241021 2211 5 1.199035522896164 2211 6 0.03139808301291325 2211 7 -19.49439685522686 2211 8 -12.390218258187895 2211 9 -8.241118253398739 2211 10 -6.347317809148429 2211 11 -8.664856726608477 2211 12 -4.230498302177438 2211 13 -6.242851805765918 2211 14 -4.250379199613729 2211 15 -4.726148000346875 2211 16 -11.01173727669815 2211 17 -7.152854202618702 2211 18 -5.3234501931660425 2211 19 -8.299564435013771 2211 20 -6.2478165730541235 2211 21 -3.9928372822915605 2211 22 -5.505231162936134 2211 23 -7.400623038495031 2211 24 -20.59987955634244 2211 25 -20.416041392251007 2212 1 -1.3657656650130114 2212 2 -13.188039419415823 2212 3 -7.637321895275141 2212 4 -18.613853625044502 2212 5 -19.14701824987053 2212 6 -19.360622732251613 2212 7 -9.504822571994888 2212 8 -9.042142788537083 2212 9 -5.156626695471434 2212 10 -5.350439646714918 2212 11 -5.541503075836832 2212 12 -3.155622425926687 2212 13 -5.636885275662376 2212 14 -3.9462596248708515 2212 15 -3.948378678749382 2212 16 -4.022777866614682 2212 17 -4.058342322229613 2212 18 -3.3524238297262805 2212 19 -3.8682429776739866 2212 20 -4.084490139461554 2212 21 -1.9759945805570318 2212 22 -3.2227640145686003 2212 23 -3.0658796161683526 2212 24 -2.3487935441791024 2212 25 -2.5446252323900147 2213 1 -17.780187906542118 2213 2 -13.188039419415823 2213 3 -8.475650817459904 2213 4 -5.677801725137181 2213 5 -19.14701824987053 2213 6 -10.225312259591743 2213 7 -19.49439685522686 2213 8 -10.297085703953343 2213 9 -8.263136809978782 2213 10 -7.348197972673969 2213 11 -6.693344284150674 2213 12 -5.222399190137846 2213 13 -17.97658988035395 2213 14 -11.788339969238363 2213 15 -6.974653932809797 2213 16 -6.212094647203589 2213 17 -4.912838030116294 2213 18 -4.8571853740657716 2213 19 -5.676128935727949 2213 20 -5.720863072213749 2213 21 -3.848926124952886 2213 22 -4.77360146179787 2213 23 -4.644628481844247 2213 24 -3.661974088316491 2213 25 -4.460527027098088 2214 1 -17.780187906542118 2214 2 -13.188039419415823 2214 3 -11.3844293392877 2214 4 -18.613853625044502 2214 5 -19.14701824987053 2214 6 -19.360622732251613 2214 7 -15.268927126625607 2214 8 -13.626231418012406 2214 9 -12.276239485656603 2214 10 -12.165522836606979 2214 11 -12.917930762390597 2214 12 -10.547034813085714 2214 13 -17.97658988035395 2214 14 -15.168898013201593 2214 15 -22.332392579705072 2214 16 -16.588984251549995 2214 17 -22.04444136653891 2214 18 -16.18252527550548 2214 19 -13.319426390844708 2214 20 -11.879966161945408 2214 21 -9.46660544992904 2214 22 -10.476348534882241 2214 23 -10.293896825493457 2214 24 -9.248105026071041 2214 25 -9.055916131290672 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 146055.0 3 4.0 4 765.0 5 2816.0 6 3518.0 7 3426.0 8 2842.0 9 2622.0 10 2513.0 11 2327.0 12 2210.0 13 2326.0 14 3389.0 15 6879.0 16 10047.0 17 10832.0 18 11133.0 19 13046.0 20 15550.0 21 17782.0 22 18051.0 23 19835.0 24 19987.0 25 20555.0 26 17234.0 27 14042.0 28 14961.0 29 16856.0 30 16537.0 31 19043.0 32 24123.0 33 29599.0 34 35240.0 35 39849.0 36 38331.0 37 38048.0 38 26018.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.727474295343086 18.019870293547097 12.742852883203518 25.509802527906306 2 12.413630877139624 20.36267903475674 41.958523812691865 25.265166275411765 3 20.672283063876584 24.465655588029158 28.85102244175014 26.011038906344115 4 11.320112543111273 16.87413602915509 37.14743007945041 34.65832134828323 5 11.804954217135624 37.819636727837995 36.853380072543146 13.522028982483228 6 30.97066891003436 36.11297665672233 18.234019539921107 14.68233489332221 7 26.36726477516716 31.14998071418757 22.23662450274885 20.246130007896422 8 25.187179845318795 38.5353359817319 20.276165387401377 16.00131878554793 9 26.185373072182056 16.300386874137846 19.469819174159262 38.044420879520835 10 14.935603540210188 30.977294892946013 33.48144974827805 20.605651818565747 11 32.99921796021134 22.80027466763309 23.74523941226213 20.45526795989344 12 23.962307127463518 25.58413280429938 31.567462182089834 18.88609788614726 13 28.950242108136848 21.041496041496043 24.545598229808757 25.462663620558356 14 20.10096123125234 24.17972803149942 27.15470680773245 28.56460392951579 15 24.205231077848932 30.899159015508065 23.048530206002624 21.847079700640382 16 20.240671656201002 28.52592472445567 30.358458650972032 20.874944968371295 17 21.50721257306288 28.532201658945795 29.460865755588568 20.499720012402754 18 21.00382484752297 27.68362612571526 31.820724714352604 19.491824312409168 19 22.822916350156483 27.14274862507976 27.226307313664122 22.808027711099633 20 22.695660133033435 28.827712248048986 28.064563079845566 20.412064539072006 21 21.166534044272904 26.831442158883718 27.297448010977376 24.704575785866005 22 21.39662228282788 29.184973922148444 27.285331417761547 22.133072377262124 23 21.218311820967592 28.52662510970857 28.138264253128003 22.116798816195832 24 22.082293215157208 28.983618241844184 27.963986200319752 20.97010234267886 25 22.35627329043699 27.24966150233054 27.846975088967973 22.547090118264496 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1036.0 1 1036.0 2 1879.5 3 2723.0 4 2723.0 5 2723.0 6 3694.0 7 4665.0 8 4665.0 9 4665.0 10 5421.5 11 6178.0 12 6178.0 13 6178.0 14 7266.5 15 8355.0 16 8355.0 17 8355.0 18 11509.5 19 14664.0 20 14664.0 21 14664.0 22 20357.5 23 26051.0 24 26051.0 25 26051.0 26 34340.5 27 42630.0 28 42630.0 29 42630.0 30 52248.5 31 61867.0 32 61867.0 33 61867.0 34 66611.0 35 71355.0 36 71355.0 37 71355.0 38 73892.5 39 76430.0 40 76430.0 41 76430.0 42 78967.0 43 81504.0 44 81504.0 45 81504.0 46 84984.5 47 88465.0 48 88465.0 49 88465.0 50 79545.0 51 70625.0 52 70625.0 53 70625.0 54 58531.0 55 46437.0 56 46437.0 57 46437.0 58 38249.0 59 30061.0 60 30061.0 61 30061.0 62 24130.5 63 18200.0 64 18200.0 65 18200.0 66 14023.5 67 9847.0 68 9847.0 69 9847.0 70 7147.5 71 4448.0 72 4448.0 73 4448.0 74 3134.0 75 1820.0 76 1820.0 77 1820.0 78 1250.0 79 680.0 80 680.0 81 680.0 82 463.0 83 246.0 84 246.0 85 246.0 86 160.0 87 74.0 88 74.0 89 74.0 90 47.0 91 20.0 92 20.0 93 20.0 94 14.0 95 8.0 96 8.0 97 8.0 98 5.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.697072521922049 2 37.032665011946605 3 5.859145320628195 4 14.281311388094691 5 13.956651121873275 6 15.346556132563125 7 9.623109826433929 8 7.8805669136777725 9 0.109516734965013 10 1.5148318873234379 11 5.874854688348587 12 6.071296591366431 13 16.699057886775854 14 1.66205110481739 15 3.0432785600045484 16 3.147259613010947 17 3.0144032460042105 18 1.5839531052931592 19 1.5217140865152283 20 0.2905484963142831 21 4.48644580791782 22 1.6936194532840807 23 0.10667408747275173 24 9.319395383839698 25 0.10996557404273845 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 668391.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.25540197598971 #Duplication Level Percentage of deduplicated Percentage of total 1 96.40110167091576 84.1151687722401 2 2.4208958925960378 4.224724885009794 3 0.48909646484377767 1.2802892593493802 4 0.21334042217147353 0.7446041717719709 5 0.11353249993169205 0.4953161959439407 6 0.07673543749972488 0.40173488669051577 7 0.04798470699773113 0.2930847428450981 8 0.035871660304851206 0.2503996911556756 9 0.02533514036018012 0.19895650706210738 >10 0.144719978496826 2.376819282213555 >50 0.015328199709047158 0.949122217949511 >100 0.013503256100114294 2.508519699135949 >500 0.002007240771449247 1.216604190425845 >1k 5.4742930130434E-4 0.9446554982065878 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2442 0.36535500926852693 No Hit CCCATGTACTCTGCGTTGATACCAC 2308 0.3453068637967896 No Hit GAGTACATGGGAAGCAGTGGTATCA 1183 0.17699220964974094 No Hit GTCCTACAGTGGACATTTCTAAATT 902 0.1349509493694559 No Hit CATGTACTCTGCGTTGATACCACTG 883 0.13210830187719463 No Hit GCGTTGATACCACTGCTTCCCATGT 840 0.12567494176312966 No Hit ACGCAGAGTACATGGGAAGCAGTGG 714 0.10682370049866022 No Hit GTATCAACGCAGAGTACATGGGAAG 694 0.10383143998049046 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 1.496130259084877E-4 0.0 0.0 6 0.0 0.0 1.496130259084877E-4 0.0 0.0 7 0.0 0.0 1.496130259084877E-4 0.0 0.0 8 0.0 0.0 1.496130259084877E-4 0.0 0.0 9 1.496130259084877E-4 0.0 1.496130259084877E-4 0.0 0.0 10 2.992260518169754E-4 0.0 1.496130259084877E-4 1.496130259084877E-4 0.0 11 2.992260518169754E-4 0.0 1.496130259084877E-4 1.496130259084877E-4 0.0 12 2.992260518169754E-4 0.0 1.496130259084877E-4 1.496130259084877E-4 0.0 13 2.992260518169754E-4 0.0 1.496130259084877E-4 1.496130259084877E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAGGA 450 0.0 17.148373 2 GTCGAGG 35 2.3610942E-4 16.966555 19 CCTACAC 80 2.0399966E-8 16.550917 3 CTGTAGG 480 0.0 16.223421 1 GTAGGAC 520 0.0 15.0655775 3 GATATAC 360 0.0 14.918089 1 TCCTACA 605 0.0 14.804897 2 GTCCTAC 570 0.0 14.604024 1 TGTCGAG 35 0.0045925453 14.550135 18 TTTAGGA 260 0.0 14.309939 2 ATGTCGA 45 1.4710345E-4 14.290801 17 TGTAGAA 405 0.0 14.290311 2 GTTGGAC 55 9.928904E-4 14.043203 3 AATACCT 45 0.005986699 13.979402 5 TAGGACC 240 0.0 13.966797 4 TAGGACG 535 0.0 13.945744 4 GGACGTG 450 0.0 13.734942 6 GGACCTG 330 0.0 13.332841 6 CTTTAGG 255 0.0 13.163019 1 AGGACGT 495 0.0 13.132164 5 >>END_MODULE