FastQCFastQC Report
Thu 26 May 2016
SRR1926575_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926575_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668391
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC70691.0576144801470995No Hit
GTACATGGGAAGCAGTGGTATCAAC63910.9561768485811448No Hit
GAGTACATGGGAAGCAGTGGTATCA29930.44779178654410373No Hit
CATGTACTCTGCGTTGATACCACTG26850.40171097456428945No Hit
GCGTTGATACCACTGCTTCCCATGT21180.31688038887417697No Hit
ACGCAGAGTACATGGGAAGCAGTGG18950.28351668409658415No Hit
CTGTAGGACGTGGAATATGGCAAGA18580.27798100213797017No Hit
GTCCTACAGTGGACATTTCTAAATT17920.26810654242800996No Hit
GTATCAACGCAGAGTACATGGGAAG17350.2595785999512261No Hit
GATATACACTGTTCTACAAATCCCG17090.2556886612776055No Hit
GCTTCCCATGTACTCTGCGTTGATA16890.2526964007594357No Hit
GTATCAACGCAGAGTACTTTTTTTT16650.249105688137632No Hit
ACTCTGCGTTGATACCACTGCTTCC15910.23803432422040394No Hit
CTTTAGGACGTGAAATATGGCGAGG12360.1849217000228908No Hit
GTCCTAAAGTGTGTATTTCTCATTT11660.17444878820929666No Hit
GTGTATATCAATGAGTTACAATGAG11520.17235420584657782No Hit
GTGTATATCAATGAGTTACAATGAA11450.1713069146652184No Hit
GGTATCAACGCAGAGTACATGGGAA11400.170558849535676No Hit
GTCCTACAGTGTGCATTTCTCATTT11380.170259623483859No Hit
TATCAACGCAGAGTACATGGGAAGC11320.1693619453284081No Hit
GGTATCAACGCAGAGTACTTTTTTT10330.1545502557634678No Hit
GATATACACTGTTCTACAATGCCGG9970.14916418683076224No Hit
GTTCTACAGTGTGGTTTTTATCATT9790.14647115236440944No Hit
CTGTAGGACCTGGAATATGGCGAGA9660.14452618302759912No Hit
CAGTGGTATCAACGCAGAGTACATG9470.14168353553533783No Hit
CTGTAGAACATATTAGATGAGTGAG9060.13554940147308986No Hit
CATGGGAAGCAGTGGTATCAACGCA8750.1309113976699267No Hit
TATCAACGCAGAGTACTTTTTTTTT7790.11654854718271192No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7690.11505241692362704No Hit
ACATGGGAAGCAGTGGTATCAACGC7600.11370589969045064No Hit
TTGTAGAACAGTGTATATCAATGAG7500.11220976943136576No Hit
CTGAAGGACCTGGAATATGGCGAGA7390.11056402614637241No Hit
GTGGTATCAACGCAGAGTACATGGG7290.10906789588728753No Hit
GCAGTGGTATCAACGCAGAGTACAT7120.10652447444684324No Hit
ATACCACTGCTTCCCATGTACTCTG7080.10592602234320929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT453.4233926E-516.9516016
TAGGACC4000.015.25873854
CCAACGA1650.014.35443719
AGGACTA400.00514161914.3050685
GTCCTAG801.2311102E-714.2996841
TCCAACG2400.014.21089318
TAGGACT852.5677036E-713.46359354
TAGGATC500.001455055913.3513974
TGTAGGA9900.013.2976042
GCCGGTT1450.013.0782511
CTGTAGG9750.012.8086051
AGGACCT6500.012.7645235
TGTACCG603.9557109E-412.7156165
GAACAGC751.4203919E-512.7137016
GTAGGAC10100.012.6545733
CCGGTTT1501.8189894E-1212.64230812
CCAACGT751.517881E-512.63190419
GATATAC6050.012.448211
CCTGAAC1154.993126E-912.4410643
GGACCTG5950.012.3397686
Produced by FastQC (version 0.11.5)