FastQCFastQC Report
Thu 26 May 2016
SRR1926574_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926574_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596451
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC28160.47212595837713406No Hit
CCCATGTACTCTGCGTTGATACCAC25250.4233373738999516No Hit
GAGTACATGGGAAGCAGTGGTATCA13450.2255005021368059No Hit
CATGTACTCTGCGTTGATACCACTG9500.15927544760592235No Hit
GCGTTGATACCACTGCTTCCCATGT9010.1510601876767748No Hit
ACGCAGAGTACATGGGAAGCAGTGG8460.14183897755222138No Hit
GTATCAACGCAGAGTACATGGGAAG8150.1366415682092913No Hit
GCTTCCCATGTACTCTGCGTTGATA6490.10881027946973011No Hit
GNACATGGGAAGCAGTGGTATCAAC6080.10193628646779031No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAT602.221966E-517.821771
CTAACAC502.5706326E-517.5238423
CTGTAGG2050.016.30041
TGTAGGA2450.016.2090032
CCCCTGT758.346942E-616.0395931
GTAGGAC2700.015.0088463
GTAAGAC450.004479790614.6032013
AGACAGT507.459934E-414.5836286
GTGTAAT751.4973621E-414.2574161
TCCAACG553.150443E-513.94073118
GCCGGTT400.00662931513.76598911
TAGGACG2350.013.7212074
TTCTATA700.001199824313.6938152
CAATACT550.001210213213.6796284
TAGGACC1201.5788828E-913.43534854
GATATAC1101.5340283E-613.3663271
GTCCTAA1603.110472E-1013.3663261
AGGACGT2200.013.2654755
GGACGTG2150.013.081666
CTTTAGG1358.33661E-812.8712781
Produced by FastQC (version 0.11.5)