FastQCFastQC Report
Thu 26 May 2016
SRR1926574_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926574_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596451
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC76751.286777958289952No Hit
GTACATGGGAAGCAGTGGTATCAAC68911.1553337994235906No Hit
GAGTACATGGGAAGCAGTGGTATCA31290.524603026904138No Hit
CATGTACTCTGCGTTGATACCACTG27980.4691081077909166No Hit
GCGTTGATACCACTGCTTCCCATGT22370.3750517645204719No Hit
ACGCAGAGTACATGGGAAGCAGTGG21520.3608008034188894No Hit
GTATCAACGCAGAGTACATGGGAAG19230.32240703762756706No Hit
GTATCAACGCAGAGTACTTTTTTTT17470.29289916522899617No Hit
GCTTCCCATGTACTCTGCGTTGATA16750.28082776288412625No Hit
ACTCTGCGTTGATACCACTGCTTCC15780.26456490139173205No Hit
TATCAACGCAGAGTACATGGGAAGC12810.21477036671914373No Hit
GGTATCAACGCAGAGTACATGGGAA11940.20018408888575925No Hit
GGTATCAACGCAGAGTACTTTTTTT10830.18157401027075148No Hit
CAGTGGTATCAACGCAGAGTACATG10460.17537065073241556No Hit
CATGGGAAGCAGTGGTATCAACGCA9530.15977842270362527No Hit
GTCCTACAGTGGACATTTCTAAATT9020.15122784604267575No Hit
CTGTAGGACGTGGAATATGGCAAGA8910.14938360401776507No Hit
ACATGGGAAGCAGTGGTATCAACGC8240.13815049350240002No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8170.13697688494109322No Hit
GTGGTATCAACGCAGAGTACATGGG8090.13563561801388546No Hit
TATCAACGCAGAGTACTTTTTTTTT7870.1319471339640641No Hit
ATACCACTGCTTCCCATGTACTCTG7730.1295999168414505No Hit
GCAGTGGTATCAACGCAGAGTACAT7730.1295999168414505No Hit
GATATACACTGTTCTACAAATCCCG7270.12188763201000585No Hit
CCACTGCTTCCCATGTACTCTGCGT6730.11283408025135341No Hit
CTGCTTCCCATGTACTCTGCGTTGA6610.11082217986054177No Hit
GGGAAGCAGTGGTATCAACGCAGAG6580.11031920476283887No Hit
CCATGTACTCTGCGTTGATACCACT6050.1014333113700874No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACC250.005914562419.0624734
CATAGAC504.4019453E-617.1605663
TCCAACG1101.2732926E-1114.64735318
CTATGGT602.4848572E-514.2968554
GTAGGAC5550.013.7421963
TGTAGGA6300.013.1644042
GTCCTAC4800.013.0999281
TAGGACC2050.013.0182744
CTGTAGG6050.012.9129281
ACTGTTC3100.012.8731318
GATATAC3750.012.7029611
CCAACGT604.1702026E-412.63587319
GGCGAGG1800.012.63587319
TCCTACA5450.012.5938492
TAGGACG5100.012.3345414
TCTAGGA701.0541882E-412.2565142
CCAACGA701.1137461E-412.18459119
GGACGTG5250.012.1616226
TATACAC3850.012.1337343
CCTACAG5600.012.0873043
Produced by FastQC (version 0.11.5)