FastQCFastQC Report
Thu 26 May 2016
SRR1926573_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926573_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786199
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC27860.35436320829713597No Hit
CCCATGTACTCTGCGTTGATACCAC25690.3267620538820324No Hit
GTCCTACAGTGGACATTTCTAAATT17630.2242434803402192No Hit
GAGTACATGGGAAGCAGTGGTATCA13200.1678964231702152No Hit
CTGTAGGACGTGGAATATGGCAAGA12230.1555585799524039No Hit
GTCCTAAAGTGTGTATTTCTCATTT10500.13355397297630753No Hit
CATGTACTCTGCGTTGATACCACTG9950.12655828867754856No Hit
GCGTTGATACCACTGCTTCCCATGT9140.11625555361937626No Hit
GTATCAACGCAGAGTACATGGGAAG8390.10671598412106857No Hit
GTCCTACAGTGTGCATTTCTCATTT8380.10658878986109116No Hit
ACGCAGAGTACATGGGAAGCAGTGG8230.10468087596142961No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTGC552.2815484E-417.041281
GTGTAGG604.42798E-415.6211751
TGTAGGA7250.015.3347152
CTGTAGG7050.015.0039231
GTATAGA902.7933365E-614.8773091
TCTATAG450.004427773414.6300653
GTGTTAG550.00447311814.6068121
TGTAGAA3600.014.4880472
GCTTATA658.127861E-414.4195451
GTATAAA751.4770353E-414.2822161
GATATAC3500.014.1546971
GTAAGAC550.001036106113.9650613
AGGACGA601.8442184E-413.9149595
GGGGTTA450.00619631213.9076246
TAGGACC2750.013.6558224
GTCCTAA4050.013.5548821
CTAGGAC901.4823454E-613.41089253
GTGTTAC600.00796228513.3895781
GTCCTAG700.001422767313.3895781
GTCCTAC10150.013.1917021
Produced by FastQC (version 0.11.5)