FastQCFastQC Report
Thu 26 May 2016
SRR1926573_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926573_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786199
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC72490.9220311905764316No Hit
GTACATGGGAAGCAGTGGTATCAAC65900.8382101732513015No Hit
CTGTAGGACGTGGAATATGGCAAGA35240.44823257216048357No Hit
GTCCTACAGTGGACATTTCTAAATT34190.43487717486285277No Hit
GAGTACATGGGAAGCAGTGGTATCA31270.3977364509494415No Hit
CATGTACTCTGCGTTGATACCACTG26940.34266133637921187No Hit
CTTTAGGACGTGAAATATGGCGAGG21940.279064206390494No Hit
GCGTTGATACCACTGCTTCCCATGT21800.27728348675080994No Hit
GTCCTAAAGTGTGTATTTCTCATTT21640.27524837859117096No Hit
ACGCAGAGTACATGGGAAGCAGTGG21390.27206852209173504No Hit
GTATCAACGCAGAGTACATGGGAAG19500.2480288069559997No Hit
GTATCAACGCAGAGTACTTTTTTTT19160.24370420211676685No Hit
GTCCTACAGTGTGCATTTCTCATTT17990.2288224736994069No Hit
GCTTCCCATGTACTCTGCGTTGATA16680.2121600256423628No Hit
CTGTAGGACCTGGAATATGGCGAGA16610.21126966582252077No Hit
GATATACACTGTTCTACAAATCCCG15830.20134851354428077No Hit
ACTCTGCGTTGATACCACTGCTTCC15230.19371685794563465No Hit
CTGAAGGACCTGGAATATGGCGAGA13520.17196663948949312No Hit
TATCAACGCAGAGTACATGGGAAGC12550.15962879627168183No Hit
GGTATCAACGCAGAGTACTTTTTTT12270.15606735699231364No Hit
GGTATCAACGCAGAGTACATGGGAA12010.15276030623290032No Hit
GTGTATATCAATGAGTTACAATGAA11220.1427119596946829No Hit
CAGTGGTATCAACGCAGAGTACATG10770.1369882179956983No Hit
GTGTATATCAATGAGTTACAATGAG9900.12592231737766138No Hit
TATCAACGCAGAGTACTTTTTTTTT9720.12363282069806752No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9290.1181634675190378No Hit
GTTCTACAGTGTGGTTTTTATCATT9110.11587397083944397No Hit
GATATACACTGTTCTACAATGCCGG8990.11434763971971473No Hit
CATGGGAAGCAGTGGTATCAACGCA8780.11167656026018857No Hit
GAATATGGCAAGAAAACTGAAAATC8540.10862389802073012No Hit
ACCTGGAATATGGCGAGAAAACTGA8350.10620720708115884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT1001.8189894E-1216.1049119
TAGGACC5600.015.66524
GTCCTAG1450.015.1182461
TCCAACG2200.015.07144118
GTAGGGA400.00514323714.304843
TTGCGAG400.005374796714.21021518
TAGTACT551.8974705E-413.8695844
CCAACGA1351.8189894E-1213.33304119
ATTGCGA500.001530297613.26286717
TTAGAAC603.9576285E-412.7154143
TAAGAGA603.9619076E-412.7137864
TGTAGGA18250.012.6465492
ACGTTTT1052.0669177E-812.63130219
ACTGTTC5850.012.5099438
CTGTAGG18450.012.5014541
TATACAC6950.012.4867193
GTCCTAC15950.012.4292311
GTAGGAC18500.012.4233043
GGCGAGG5750.012.19195319
GATATAC6350.012.1577411
Produced by FastQC (version 0.11.5)