FastQCFastQC Report
Thu 26 May 2016
SRR1926572_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926572_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576624
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC21720.3766752684591692No Hit
CCCATGTACTCTGCGTTGATACCAC20610.35742528926995754No Hit
GTCCTACAGTGGACATTTCTAAATT11850.20550653458753018No Hit
GAGTACATGGGAAGCAGTGGTATCA11240.19492771719526067No Hit
CTGTAGGACGTGGAATATGGCAAGA8880.1539998335136935No Hit
CATGTACTCTGCGTTGATACCACTG7650.13266877549321568No Hit
ACGCAGAGTACATGGGAAGCAGTGG7190.12469130664002886No Hit
GCGTTGATACCACTGCTTCCCATGT7160.12417103693221232No Hit
GTCCTAAAGTGTGTATTTCTCATTT6830.11844807014623049No Hit
GTCCTACAGTGTGCATTTCTCATTT5980.10370709509142874No Hit
CTTTAGGACGTGAAATATGGCGAGG5950.10318682538361221No Hit
GTATCAACGCAGAGTACATGGGAAG5910.10249313243985683No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTGA501.0997456E-418.7435931
TAGGGCG300.00835957417.9512464
CTACCCT502.9673876E-517.2331964
GTATTAC654.3537246E-516.4778841
TGTAGGA6950.016.4063132
CTGTAGG7100.016.2167911
GTCCTAA3200.015.48021
TAGGACC2800.015.0021134
TCCTAGA550.003768057814.986712
TCTACCC450.00435943814.66295053
TCCTAAA3500.014.5228342
CTCTTCG458.619115E-414.3085079
TCTATAC857.574672E-714.23168853
GTCCTAC6550.014.1036841
GGTCCTA808.855841E-714.036647
TCTAGGA600.00671280913.7378162
GTAGGAC7550.013.6918953
TCCGTTG400.007252630313.58023912
GTCTTAC700.001422366813.3882811
TTCTATA1051.0531088E-513.0836342
Produced by FastQC (version 0.11.5)