##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926572_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 576624 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.058944823663253 26.0 2.0 30.0 2.0 31.0 2 15.618487957490496 15.0 2.0 30.0 2.0 31.0 3 18.92890341019451 16.0 10.0 30.0 2.0 31.0 4 20.75884978772996 27.0 2.0 33.0 2.0 35.0 5 21.576176156386136 28.0 2.0 35.0 2.0 37.0 6 22.14628770221149 30.0 2.0 35.0 2.0 37.0 7 21.921550264990703 29.0 2.0 35.0 2.0 37.0 8 22.09794424096118 29.0 2.0 35.0 2.0 37.0 9 24.638025819251368 31.0 10.0 37.0 2.0 39.0 10 25.25397312633536 31.0 10.0 37.0 2.0 39.0 11 22.506593551430395 29.0 2.0 37.0 2.0 39.0 12 23.53587953328339 30.0 2.0 37.0 2.0 39.0 13 20.38194560059935 25.0 2.0 35.0 2.0 39.0 14 23.56127563195427 29.0 2.0 37.0 2.0 40.0 15 24.602193110241682 30.0 2.0 38.0 2.0 40.0 16 24.952719623185992 31.0 2.0 38.0 2.0 40.0 17 24.302555911651268 30.0 2.0 38.0 2.0 40.0 18 24.38313701823025 30.0 2.0 37.0 2.0 40.0 19 24.721008837648103 30.0 2.0 38.0 2.0 40.0 20 24.96725249035767 31.0 2.0 38.0 2.0 40.0 21 22.9000665945226 30.0 2.0 37.0 2.0 40.0 22 23.795181955659146 30.0 2.0 37.0 2.0 40.0 23 23.763764602236467 30.0 2.0 37.0 2.0 40.0 24 22.862347040705902 30.0 2.0 38.0 2.0 40.0 25 22.66407572352174 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.482372083041856 1101 2 9.83914268314625 1101 3 6.582024285861685 1101 4 7.668012481837781 1101 5 7.735115765570413 1101 6 7.49556594445335 1101 7 7.085875994243473 1101 8 5.435210329811195 1101 9 5.43281463841258 1101 10 5.171588891619024 1101 11 7.652653725502969 1101 12 5.254430086666826 1101 13 9.67766931000073 1101 14 7.305911647115227 1101 15 6.166698793920638 1101 16 5.77982432722974 1101 17 2.4951169366934245 1101 18 4.395914256897321 1101 19 5.1187837274791335 1101 20 2.9993823115625737 1101 21 0.5805591093217615 1101 22 4.029226281216729 1101 23 5.027514113067767 1101 24 6.377185180687345 1101 25 6.807876658296053 1102 1 5.435135560883648 1102 2 10.024246409405315 1102 3 6.276423944123643 1102 4 7.756858896874256 1102 5 7.7269184337559125 1102 6 6.606270450576158 1102 7 6.284049010081716 1102 8 6.366114621252407 1102 9 5.558685960895001 1102 10 4.8096171069139935 1102 11 7.4092091812632255 1102 12 4.9287077269218145 1102 13 9.083993654455153 1102 14 6.860861372191351 1102 15 4.811699779703456 1102 16 5.352695583993306 1102 17 6.2017950676491935 1102 18 6.371430697564147 1102 19 4.847356094891346 1102 20 5.2152928462949895 1102 21 4.153652714877197 1102 22 5.09362747291863 1102 23 5.572020016811553 1102 24 6.9307175691295875 1102 25 7.032714004130572 1103 1 4.408880222809199 1103 2 8.61048248939115 1103 3 5.7253112065820275 1103 4 7.392594957429289 1103 5 6.825195137082037 1103 6 6.33216977308869 1103 7 6.24928945552217 1103 8 5.764645868148875 1103 9 5.056820603432925 1103 10 4.62398273424839 1103 11 4.615231867809769 1103 12 3.6853195987130327 1103 13 7.548923206985261 1103 14 5.863648613491744 1103 15 5.106526651214008 1103 16 4.801814936645179 1103 17 2.7687806154468184 1103 18 1.2042858171499695 1103 19 3.533863774378027 1103 20 3.8876656932536378 1103 21 5.946178115259766 1103 22 1.8176117814625705 1103 23 4.066386162300798 1103 24 5.247646983485069 1103 25 2.136280204889669 1104 1 5.035894386922454 1104 2 9.83538016703774 1104 3 6.452814371108719 1104 4 6.752456111495771 1104 5 7.013811556060055 1104 6 6.6431829636649375 1104 7 6.626817755584131 1104 8 6.469311299826455 1104 9 5.466107891441521 1104 10 4.178056019478792 1104 11 7.151389308725317 1104 12 4.341712048912257 1104 13 8.370910476607186 1104 14 6.135480127820063 1104 15 5.597115970577722 1104 16 5.175950736555688 1104 17 5.346145454818526 1104 18 3.3016809679578643 1104 19 4.068570475097232 1104 20 3.2234477485007993 1104 21 2.6465115198015923 1104 22 4.463912819087227 1104 23 4.748175599849354 1104 24 5.947557388321293 1104 25 4.974830357456522 1105 1 3.492443323301355 1105 2 9.135691005048255 1105 3 6.187976003743895 1105 4 5.660909674133439 1105 5 2.3827623969086993 1105 6 4.514484132202995 1105 7 5.734821182210606 1105 8 3.5276359465990694 1105 9 3.9856570876346673 1105 10 3.531831203983426 1105 11 6.632941975369029 1105 12 3.95605275199674 1105 13 4.610064796879897 1105 14 3.9168761755131953 1105 15 4.358056602500234 1105 16 4.240085205559016 1105 17 4.8473939196710845 1105 18 2.616784523239346 1105 19 2.4100561326203156 1105 20 1.8434908004521695 1105 21 2.351663041593124 1105 22 4.102337989975091 1105 23 1.3532735936451168 1105 24 4.53793779185769 1105 25 5.148529225438537 1106 1 6.5616953234411 1106 2 11.108649814397378 1106 3 7.818245367542236 1106 4 9.119470970992609 1106 5 8.836397585393485 1106 6 7.678827492437772 1106 7 7.464402288314091 1106 8 7.278235115099925 1106 9 6.477128980867381 1106 10 5.665478969766188 1106 11 8.017206378122061 1106 12 5.553509342850251 1106 13 4.8705406379867 1106 14 5.67725768688517 1106 15 5.979759428898586 1106 16 5.89991820699143 1106 17 6.4892623471594675 1106 18 6.4418482831527015 1106 19 6.081257613946523 1106 20 5.753695880283075 1106 21 5.894499222286971 1106 22 6.231248306853125 1106 23 6.711118683258366 1106 24 7.4063652802411575 1106 25 6.237108340136508 1107 1 3.605723698606486 1107 2 -13.201695013188829 1107 3 -5.261694288850718 1107 4 1.522925593674188 1107 5 4.298862983021728 1107 6 4.651174888044796 1107 7 4.584868828369842 1107 8 5.0526564560364235 1107 9 3.589386642210858 1107 10 3.0205171239157877 1107 11 5.40331660773046 1107 12 2.8886311773740196 1107 13 4.27141356977792 1107 14 3.883438512655207 1107 15 3.6062735115146474 1107 16 3.3674415315531334 1107 17 3.8683100313026735 1107 18 -1.05397599782588 1107 19 1.8083971023928669 1107 20 2.0847791847302446 1107 21 -1.114798380079904 1107 22 1.9674295720183643 1107 23 -1.0809370515812802 1107 24 2.4867550726025165 1107 25 3.084391169060428 1108 1 5.144524586220257 1108 2 10.044002883560694 1108 3 0.29521039499846324 1108 4 5.5659883159803165 1108 5 6.856401541350497 1108 6 6.966741101871786 1108 7 6.98382980482079 1108 8 6.615850614916255 1108 9 5.363313271950467 1108 10 4.4210177805301605 1108 11 6.694048017395293 1108 12 1.9881371532280454 1108 13 3.2021364898832054 1108 14 4.334024728111892 1108 15 4.430088621035651 1108 16 4.591973326659129 1108 17 0.05550955679693459 1108 18 3.4762472131605193 1108 19 4.051749000321074 1108 20 4.365876460301724 1108 21 4.624394012668343 1108 22 1.8090026419226852 1108 23 4.185100730748687 1108 24 4.741529028604614 1108 25 5.278191895599967 1109 1 3.897337867198516 1109 2 -13.201695013188829 1109 3 -5.154447105509204 1109 4 2.3349434060234024 1109 5 2.3401069294596226 1109 6 2.854836153271844 1109 7 3.888385339600525 1109 8 4.34921873099815 1109 9 3.6490796887040915 1109 10 3.026667866079638 1109 11 1.3844433544964225 1109 12 1.8133809843165842 1109 13 2.656865636821216 1109 14 3.7768603866619124 1109 15 3.8402174378391365 1109 16 3.510991849980659 1109 17 4.096234525783991 1109 18 4.363974331439962 1109 19 2.708625228021411 1109 20 1.767371917433497 1109 21 1.241431929885401 1109 22 3.311802351597315 1109 23 3.526787955427146 1109 24 4.596209519905912 1109 25 4.754482325518762 1110 1 3.136820222997404 1110 2 9.070848046588274 1110 3 6.241674078718564 1110 4 7.5808691472844 1110 5 8.19228620643251 1110 6 3.900502369285064 1110 7 6.407566876232895 1110 8 7.386805146193801 1110 9 5.621928617523217 1110 10 5.337302328603489 1110 11 3.0696187737479548 1110 12 5.860814656758645 1110 13 10.109558211266929 1110 14 7.767940005300428 1110 15 7.193922863898411 1110 16 6.910804557129431 1110 17 7.256715094949708 1110 18 7.42124247663347 1110 19 6.1972901751735066 1110 20 6.524341864281958 1110 21 4.617113902788663 1110 22 6.723411951474585 1110 23 7.059453938123333 1110 24 8.00602289824067 1110 25 8.225530238609764 1111 1 4.85486914469374 1111 2 9.044174763293192 1111 3 7.140439663980025 1111 4 7.862716840769089 1111 5 7.865424762766896 1111 6 7.098753098148038 1111 7 6.964935112281115 1111 8 7.216447641489793 1111 9 5.590871817903675 1111 10 4.952607417204277 1111 11 6.7340523283248 1111 12 6.0893157836531735 1111 13 7.869126476262895 1111 14 6.093436769355595 1111 15 5.538855973920533 1111 16 5.502083013800853 1111 17 6.4963197514019555 1111 18 6.218008599495988 1111 19 5.302577274815796 1111 20 4.899649434118402 1111 21 7.336568858023039 1111 22 6.858184401488025 1111 23 6.527539044506312 1111 24 6.241223361404657 1111 25 6.93799638008155 1112 1 -1.904992112061926 1112 2 7.015696291158996 1112 3 5.359915766109189 1112 4 -0.5460005140440956 1112 5 3.402419120080687 1112 6 2.4189947464730928 1112 7 5.379200084221331 1112 8 5.28682757211817 1112 9 4.193664544254521 1112 10 3.3656931483911627 1112 11 -0.2898167336986539 1112 12 3.6089134589740084 1112 13 7.481450118311471 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7 4.504191416306483 2204 8 4.8699090260436115 2204 9 3.254963018293605 2204 10 2.917068389563145 2204 11 4.5543443549098725 2204 12 4.706991425338419 2204 13 2.717137861268025 2204 14 4.012255424637257 2204 15 4.0652469814513665 2204 16 3.92152597895684 2204 17 -1.1682678881651505 2204 18 2.3425727108548173 2204 19 3.25625799522426 2204 20 3.398501606566331 2204 21 5.028737454785052 2204 22 4.648365135435739 2204 23 3.7660765647775776 2204 24 5.061888469553178 2204 25 5.279727057314847 2205 1 5.664555407258469 2205 2 9.782384588801222 2205 3 6.974366241007424 2205 4 7.618120580089979 2205 5 7.973093416582369 2205 6 6.986785240642561 2205 7 7.113408488838562 2205 8 6.854339813283882 2205 9 5.816545200263015 2205 10 5.246960723558804 2205 11 7.385341074100296 2205 12 6.4847496928245185 2205 13 9.355583404449948 2205 14 7.228893596009499 2205 15 6.016496923690635 2205 16 5.2208651961834 2205 17 6.06846485677886 2205 18 5.953810638861842 2205 19 4.798146078030044 2205 20 4.943186849084391 2205 21 -1.188720148837703 2205 22 3.373879976093132 2205 23 4.569187942871629 2205 24 6.2158400737612425 2205 25 6.650886738783665 2206 1 4.354889310862976 2206 2 8.735063880091804 2206 3 6.314461220654643 2206 4 1.8571615412918732 2206 5 5.84807129399373 2206 6 6.083026367026452 2206 7 6.3880933648537415 2206 8 6.156392789509475 2206 9 4.888328575875072 2206 10 4.282689195821991 2206 11 4.1350844520721 2206 12 5.171166423400884 2206 13 8.077299920683014 2206 14 0.8250029700877235 2206 15 3.054439621269932 2206 16 3.738224054067892 2206 17 4.958856770726907 2206 18 -0.908589809489289 2206 19 1.0865361435129053 2206 20 2.822896994011927 2206 21 3.7574531349410343 2206 22 -1.4832739774592625 2206 23 -2.086897243843019 2206 24 1.774504262697981 2206 25 3.4569247306425517 2207 1 0.5877770571212473 2207 2 -13.201695013188829 2207 3 -6.213003693978413 2207 4 -4.984785244768034 2207 5 0.2681813985016781 2207 6 0.8580806808456742 2207 7 2.1017176803441586 2207 8 2.4908240587233514 2207 9 0.07455016071151022 2207 10 -0.3100154574828906 2207 11 -1.864991334652398 2207 12 -2.6543365866923523 2207 13 -0.20664087239590856 2207 14 -5.192632989352777 2207 15 -1.8318278242510715 2207 16 -0.6958638429865331 2207 17 0.21412006158080743 2207 18 0.15187342228983525 2207 19 -0.3552169005871946 2207 20 -0.3816902403944695 2207 21 -3.9234682373039256 2207 22 -4.691336713321153 2207 23 -1.996130010136195 2207 24 -0.2718101705922109 2207 25 0.177158037159046 2208 1 5.076656132041464 2208 2 6.673544910035741 2208 3 6.413702190816917 2208 4 8.353745717359484 2208 5 3.927257337858901 2208 6 6.318517175975494 2208 7 6.779202360172867 2208 8 6.669540202890552 2208 9 5.208217357709945 2208 10 4.857848702092237 2208 11 7.049227973576048 2208 12 6.633527874851044 2208 13 8.927005564284176 2208 14 3.53938432852733 2208 15 4.96038592398666 2208 16 5.465859036960321 2208 17 6.227202836744674 2208 18 1.2497083098160928 2208 19 3.7011454764315097 2208 20 4.814843918822074 2208 21 7.441731241045893 2208 22 6.241679175871592 2208 23 2.412010299419027 2208 24 3.8487742512423573 2208 25 5.1640875294560225 2209 1 3.698631274170154 2209 2 5.337844526350711 2209 3 5.623292779486203 2209 4 1.5321121836338207 2209 5 5.549117201561376 2209 6 6.022767491550184 2209 7 6.54928914561474 2209 8 5.16850173640103 2209 9 4.8048379706453375 2209 10 4.101537688583527 2209 11 0.0027525153923342316 2209 12 3.9088992154815045 2209 13 2.786405271092711 2209 14 4.278289260330535 2209 15 4.59745101210741 2209 16 4.212513560966094 2209 17 1.5548856058861773 2209 18 3.762404307157002 2209 19 4.545878885464344 2209 20 4.5519956578931975 2209 21 6.487075049345556 2209 22 5.040208991675879 2209 23 1.0266095435768108 2209 24 -2.2336392563151044 2209 25 0.46008698380480695 2210 1 -2.081662761937835 2210 2 -13.201695013188829 2210 3 -7.210380216977493 2210 4 -2.1400578722571666 2210 5 -0.4006877831273279 2210 6 -1.038790895589596 2210 7 -0.9533212872282526 2210 8 -19.62028705238776 2210 9 -12.135892400317593 2210 10 -6.87660853374874 2210 11 -9.694495472553328 2210 12 -4.481811791508573 2210 13 -4.894227793261283 2210 14 -3.668493674824493 2210 15 -5.421661123283794 2210 16 -4.927347230057489 2210 17 -4.435389023923339 2210 18 -4.872947746799703 2210 19 -9.716984400654756 2210 20 -7.107059803487843 2210 21 -5.036668567421316 2210 22 -9.415919441196738 2210 23 -7.617230914217135 2210 24 -10.29363159537721 2210 25 -8.248677806355333 2211 1 -0.1280736086787968 2211 2 4.2739405800315104 2211 3 0.512843347002871 2211 4 1.3626005109441799 2211 5 0.717322483114117 2211 6 -0.6211380665502872 2211 7 -19.46072086439527 2211 8 -12.83956671340471 2211 9 -8.993734966698787 2211 10 -7.484666099950768 2211 11 -9.367406838877189 2211 12 -5.128730575326255 2211 13 -6.744901459366561 2211 14 -5.004312197059058 2211 15 -5.615960492617653 2211 16 -10.840211833920318 2211 17 -7.655759361114722 2211 18 -6.166088804598822 2211 19 -8.582134721362822 2211 20 -7.064722755435383 2211 21 -4.940259064429235 2211 22 -6.549420892445529 2211 23 -8.507469026518708 2211 24 -20.445851468527316 2211 25 -20.2476207239029 2212 1 -1.202811537385017 2212 2 -13.201695013188829 2212 3 -7.4789504238805975 2212 4 -18.394814743293107 2212 5 -19.101533449089274 2212 6 -19.67092620214351 2212 7 -9.387176431300276 2212 8 -8.82049134247969 2212 9 -5.0166321204876745 2212 10 -5.22992349681024 2212 11 -5.208011875147335 2212 12 -2.6846647263970453 2212 13 -5.392089873694568 2212 14 -3.684112364992437 2212 15 -3.407455835496066 2212 16 -3.6676137573634726 2212 17 -3.7346858859325067 2212 18 -2.7693061571377307 2212 19 -3.509036090798947 2212 20 -3.3460198243919486 2212 21 -1.5399110787641668 2212 22 -2.9272385375694014 2212 23 -3.0734629497541093 2212 24 -2.222153817863372 2212 25 -2.5377126544442703 2213 1 -17.67574310020422 2213 2 -13.201695013188829 2213 3 -8.719460802120883 2213 4 -6.2886576307665685 2213 5 -19.101533449089274 2213 6 -10.678357200020367 2213 7 -19.46072086439527 2213 8 -10.578885778502409 2213 9 -8.675814656504105 2213 10 -7.6579383479625704 2213 11 -7.053791289559113 2213 12 -5.503449438905367 2213 13 -17.87625739156995 2213 14 -12.003977082445362 2213 15 -7.380087035494331 2213 16 -6.794107709986388 2213 17 -5.49171513498208 2213 18 -5.513553178991565 2213 19 -6.323570181436953 2213 20 -6.368278119068478 2213 21 -4.527091983929758 2213 22 -5.414911734145484 2213 23 -5.1520181286814335 2213 24 -4.429927901648334 2213 25 -5.234881870399718 2214 1 -17.67574310020422 2214 2 -13.201695013188829 2214 3 -11.724565137602486 2214 4 -18.394814743293107 2214 5 -19.101533449089274 2214 6 -19.67092620214351 2214 7 -15.568413172087578 2214 8 -14.061707170730955 2214 9 -13.106390417506129 2214 10 -13.01426568875831 2214 11 -13.561987372424475 2214 12 -11.552713876900821 2214 13 -17.87625739156995 2214 14 -15.607121594311089 2214 15 -22.2123587977024 2214 16 -16.963538230470313 2214 17 -21.9205898695893 2214 18 -16.534628820645356 2214 19 -13.808636576743426 2214 20 -12.71914056708918 2214 21 -10.31000131696158 2214 22 -11.336962262418451 2214 23 -11.222475043969315 2214 24 -10.180762711130866 2214 25 -9.935194688399942 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 126494.0 3 14.0 4 544.0 5 2146.0 6 3284.0 7 3006.0 8 2550.0 9 2341.0 10 2186.0 11 2032.0 12 1898.0 13 2011.0 14 2970.0 15 6011.0 16 8537.0 17 9133.0 18 9543.0 19 11216.0 20 13285.0 21 15421.0 22 15540.0 23 16815.0 24 17700.0 25 17689.0 26 14859.0 27 12132.0 28 13071.0 29 14638.0 30 14405.0 31 16414.0 32 20570.0 33 25361.0 34 30441.0 35 34045.0 36 33009.0 37 32896.0 38 22417.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.65116236975003 18.27471707524079 12.550656956620552 25.52346359838863 2 12.060545935711165 20.205062532805336 44.38139102858289 23.35300050290061 3 20.824021831323524 25.379651141350447 27.823136777667145 25.973190249658884 4 11.995788749076258 17.275644594717715 37.11833007703755 33.61023657916848 5 11.690008434843351 38.3155275601964 36.8556353409871 13.138828663973154 6 31.034836065573774 37.45450819672131 18.10983606557377 13.400819672131147 7 26.535075518790013 31.333631328643424 21.156771751579342 20.97452140098722 8 24.887257256055467 39.921999279647025 19.82096227151304 15.369781192784465 9 26.45097559239526 16.414847684671592 19.42330762192952 37.71086910100363 10 15.243184239020396 31.06596124897665 33.27094905675326 20.419905455249697 11 32.917949947439276 22.809508879995573 24.02707338214411 20.24546779042104 12 24.94208964928679 25.447673795704844 31.509880780413557 18.10035577459481 13 29.52447791281748 21.81499745932843 24.253417411513826 24.40710721634027 14 19.684292382213584 25.130001922035877 26.861244879714057 28.324460816036478 15 23.985474840348463 32.672307389585534 22.552278053056188 20.78993971700982 16 19.515198814309905 28.657041287934387 31.74459691190738 20.08316298584833 17 20.856039618480754 29.49852718405102 30.036324094625073 19.609109102843153 18 20.562379321522698 27.63801389652305 33.172487421250686 18.627119360703563 19 23.003891324635077 27.25353476660856 27.242794006303594 22.49977990245277 20 22.798690727960818 29.154260351736422 28.52675589937667 19.52029302092609 21 20.556262497727687 27.216506089801857 27.21377931285221 25.01345209961825 22 21.36997220693839 30.41664903147529 27.03845177127862 21.1749269903077 23 20.358686782756646 29.62117397871491 28.72515147832428 21.29498776020417 24 22.35596295235367 29.003341926859544 28.82268690919507 19.81800821159171 25 21.657809252800025 27.985839712349623 28.537077001338595 21.819274033511753 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1047.0 1 1047.0 2 1789.5 3 2532.0 4 2532.0 5 2532.0 6 3475.0 7 4418.0 8 4418.0 9 4418.0 10 4994.5 11 5571.0 12 5571.0 13 5571.0 14 6526.5 15 7482.0 16 7482.0 17 7482.0 18 10337.0 19 13192.0 20 13192.0 21 13192.0 22 18223.0 23 23254.0 24 23254.0 25 23254.0 26 30948.0 27 38642.0 28 38642.0 29 38642.0 30 47465.5 31 56289.0 32 56289.0 33 56289.0 34 60425.0 35 64561.0 36 64561.0 37 64561.0 38 66075.5 39 67590.0 40 67590.0 41 67590.0 42 69233.0 43 70876.0 44 70876.0 45 70876.0 46 73601.0 47 76326.0 48 76326.0 49 76326.0 50 67449.5 51 58573.0 52 58573.0 53 58573.0 54 47493.0 55 36413.0 56 36413.0 57 36413.0 58 29789.5 59 23166.0 60 23166.0 61 23166.0 62 18518.5 63 13871.0 64 13871.0 65 13871.0 66 10630.0 67 7389.0 68 7389.0 69 7389.0 70 5366.5 71 3344.0 72 3344.0 73 3344.0 74 2339.5 75 1335.0 76 1335.0 77 1335.0 78 923.5 79 512.0 80 512.0 81 512.0 82 330.5 83 149.0 84 149.0 85 149.0 86 108.0 87 67.0 88 67.0 89 67.0 90 42.5 91 18.0 92 18.0 93 18.0 94 11.5 95 5.0 96 5.0 97 5.0 98 3.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.77101889619579 2 36.89319209744998 3 5.945642220927329 4 14.34366242126585 5 13.852180970615166 6 15.369460861844114 7 9.601230611282222 8 7.552928771608536 9 0.10665529010238908 10 1.4982033352756736 11 5.964718776880602 12 6.116637531563029 13 16.724243180998364 14 1.6504689364299787 15 3.051208413108022 16 3.1150281639335162 17 3.033345819806321 18 1.5608091234495962 19 1.50774161325231 20 0.28580149282721495 21 4.599877910041899 22 1.6607009073503705 23 0.10995033158522712 24 9.186922500624323 25 0.11220483365243208 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 576624.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.54785283849965 #Duplication Level Percentage of deduplicated Percentage of total 1 96.40095841576074 82.46895004041825 2 2.286795858411509 3.9126095113415653 3 0.5200257618372023 1.3346126203763293 4 0.22472914160208018 0.7690038213718842 5 0.13572558592568687 0.58055162255949 6 0.08217719082374497 0.42180493363626226 7 0.061520832974854237 0.3684082616084323 8 0.04082350029348095 0.2793890236367324 9 0.03189479703762886 0.245567826296 >10 0.17637999352644776 3.006511498224217 >50 0.01796901747427334 1.0675277518869262 >100 0.01840197802322245 3.304627945582123 >500 0.0017319508727690444 1.028818485550823 >1k 8.659754363845222E-4 1.2116166575109781 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2172 0.3766752684591692 No Hit CCCATGTACTCTGCGTTGATACCAC 2061 0.35742528926995754 No Hit GTCCTACAGTGGACATTTCTAAATT 1185 0.20550653458753018 No Hit GAGTACATGGGAAGCAGTGGTATCA 1124 0.19492771719526067 No Hit CTGTAGGACGTGGAATATGGCAAGA 888 0.1539998335136935 No Hit CATGTACTCTGCGTTGATACCACTG 765 0.13266877549321568 No Hit ACGCAGAGTACATGGGAAGCAGTGG 719 0.12469130664002886 No Hit GCGTTGATACCACTGCTTCCCATGT 716 0.12417103693221232 No Hit GTCCTAAAGTGTGTATTTCTCATTT 683 0.11844807014623049 No Hit GTCCTACAGTGTGCATTTCTCATTT 598 0.10370709509142874 No Hit CTTTAGGACGTGAAATATGGCGAGG 595 0.10318682538361221 No Hit GTATCAACGCAGAGTACATGGGAAG 591 0.10249313243985683 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTCTGA 50 1.0997456E-4 18.743593 1 TAGGGCG 30 0.008359574 17.951246 4 CTACCCT 50 2.9673876E-5 17.233196 4 GTATTAC 65 4.3537246E-5 16.477884 1 TGTAGGA 695 0.0 16.406313 2 CTGTAGG 710 0.0 16.216791 1 GTCCTAA 320 0.0 15.4802 1 TAGGACC 280 0.0 15.002113 4 TCCTAGA 55 0.0037680578 14.98671 2 TCTACCC 45 0.004359438 14.6629505 3 TCCTAAA 350 0.0 14.522834 2 CTCTTCG 45 8.619115E-4 14.308507 9 TCTATAC 85 7.574672E-7 14.2316885 3 GTCCTAC 655 0.0 14.103684 1 GGTCCTA 80 8.855841E-7 14.03664 7 TCTAGGA 60 0.006712809 13.737816 2 GTAGGAC 755 0.0 13.691895 3 TCCGTTG 40 0.0072526303 13.580239 12 GTCTTAC 70 0.0014223668 13.388281 1 TTCTATA 105 1.0531088E-5 13.083634 2 >>END_MODULE