FastQCFastQC Report
Thu 26 May 2016
SRR1926572_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926572_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576624
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC64881.1251699547712202No Hit
GTACATGGGAAGCAGTGGTATCAAC57580.9985709925358639No Hit
GAGTACATGGGAAGCAGTGGTATCA26700.4630400399567135No Hit
GTCCTACAGTGGACATTTCTAAATT26180.4540220316878936No Hit
CTGTAGGACGTGGAATATGGCAAGA25610.44413690723937954No Hit
CATGTACTCTGCGTTGATACCACTG23470.407024334748467No Hit
GCGTTGATACCACTGCTTCCCATGT18750.32516856738533256No Hit
CTTTAGGACGTGAAATATGGCGAGG17570.30470462554454897No Hit
ACGCAGAGTACATGGGAAGCAGTGG17300.30002219817420017No Hit
GTATCAACGCAGAGTACTTTTTTTT16780.2910041899053803No Hit
GTCCTAAAGTGTGTATTTCTCATTT16300.2826798745803158No Hit
GTATCAACGCAGAGTACATGGGAAG16080.2788645633896612No Hit
GATATACACTGTTCTACAAATCCCG15960.2767834845583951No Hit
GTCCTACAGTGTGCATTTCTCATTT14540.25215738505507923No Hit
ACTCTGCGTTGATACCACTGCTTCC13760.2386303726518494No Hit
GCTTCCCATGTACTCTGCGTTGATA13500.23412136851743942No Hit
CTGTAGGACCTGGAATATGGCGAGA11890.20620022753128556No Hit
GGTATCAACGCAGAGTACTTTTTTT11030.19128582924054496No Hit
GGTATCAACGCAGAGTACATGGGAA10960.19007186658897304No Hit
GTGTATATCAATGAGTTACAATGAA10910.18920475040927884No Hit
GTGTATATCAATGAGTTACAATGAG10590.18365520685923584No Hit
TATCAACGCAGAGTACATGGGAAGC10410.18053358861233665No Hit
CTGAAGGACCTGGAATATGGCGAGA10060.1744637753544771No Hit
CAGTGGTATCAACGCAGAGTACATG9170.15902910735591996No Hit
GATATACACTGTTCTACAATGCCGG8770.1520921779183662No Hit
GTTCTACAGTGTGGTTTTTATCATT8670.15035794555897777No Hit
TATCAACGCAGAGTACTTTTTTTTT8540.1481034434917728No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8220.1425538999417298No Hit
CATGGGAAGCAGTGGTATCAACGCA7440.12902688753849995No Hit
ACATGGGAAGCAGTGGTATCAACGC7020.1217431116290685No Hit
GTGGTATCAACGCAGAGTACATGGG6850.11879491661810818No Hit
CTGTAGAACATATTAGATGAGTGAG6820.11827464691029163No Hit
TTGTAGAACAGTGTATATCAATGAG6780.11758095396653626No Hit
ACCTGGAATATGGCGAGAAAACTGA6760.11723410749465857No Hit
GCAGTGGTATCAACGCAGAGTACAT6670.11567329837120896No Hit
GTTCTACAAATCCCGTTTCCAACGA6020.10440078803518411No Hit
CCATGTACTCTGCGTTGATACCACT5930.10283997891173452No Hit
GTCCTTCAGTGTGCATTTCTCATTT5860.10162601626016261No Hit
ATACCACTGCTTCCCATGTACTCTG5770.10006520713671299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT951.8189894E-1115.9512919
TCCAACG2350.015.71795918
GTCGAGA508.927963E-515.15372619
CCAACGA1700.014.48517719
CTTAGAC400.005114787314.3158693
GTCTAGA602.4678016E-514.3071191
GTGTAGG602.4678016E-514.3071191
GTCCTAG954.609319E-914.0561161
GCAGGAC551.8816709E-413.8820563
GATATAC5500.013.8735691
TGTCGAG552.008052E-413.776113518
TGGACTG655.223169E-513.2111855
TAGGACC4600.013.0675854
CTATAGG905.130005E-712.7174391
TGTAGGA11250.012.6392782
CTGTAGG11200.012.5187281
ACTGTTC6150.012.5131698
TCCTAGA1001.3540375E-712.4060032
TATACAC6400.012.3772623
CGGTTTC1800.012.11138613
Produced by FastQC (version 0.11.5)