FastQCFastQC Report
Thu 26 May 2016
SRR1926571_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926571_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502657
Sequences flagged as poor quality0
Sequence length25
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC27090.5389360935986169No Hit
CCCATGTACTCTGCGTTGATACCAC26440.5260048104373359No Hit
GAGTACATGGGAAGCAGTGGTATCA14800.294435370441474No Hit
GCGTTGATACCACTGCTTCCCATGT10640.2116751582092759No Hit
CATGTACTCTGCGTTGATACCACTG10040.19973858913732426No Hit
GTCCTACAGTGGACATTTCTAAATT10000.1989428178658608No Hit
GTATCAACGCAGAGTACATGGGAAG9000.17904853607927473No Hit
ACGCAGAGTACATGGGAAGCAGTGG8360.16631619573585965No Hit
CTGTAGGACGTGGAATATGGCAAGA7210.14343777168128563No Hit
GCTTCCCATGTACTCTGCGTTGATA6850.13627583023811465No Hit
ACTCTGCGTTGATACCACTGCTTCC6550.13030754570213882No Hit
GTCCTAAAGTGTGTATTTCTCATTT6320.12573186089122404No Hit
CNCATGTACTCTGCGTTGATACCAC6060.12055934762671165No Hit
GNACATGGGAAGCAGTGGTATCAAC5660.11260163491207723No Hit
CTTTAGGACGTGAAATATGGCGAGG5640.11220374927634551No Hit
GGTATCAACGCAGAGTACATGGGAA5560.11061220673341862No Hit
TATCAACGCAGAGTACATGGGAAGC5360.1066333503761014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAGA552.3141199E-417.006191
GTCCTAT654.4246357E-516.4455451
TGTAGAA1950.015.4481752
CTTAGGG505.1346794E-415.3182443
TTTAGGA3000.014.6055482
GTGTAAG550.004529550714.5767351
TCCGTTG501.3104582E-414.48432512
GTCCTAC7050.014.4044311
CAGGACT450.00513825914.3038744
TGTAGGA6550.014.21532252
CTGTAGG6450.013.8799131
AGGGCTG601.9022636E-413.8617465
TAGGACC2250.013.8270784
TGGCTAG752.1855521E-713.64010718
TCCTACA8050.013.6076532
AAGTGGT400.00725491113.57905513
CCAACGA701.5099977E-613.38756519
GTACATA904.6791087E-513.3620061
CTTTAGG3100.013.3620061
GCTCAGG600.0080623113.3620051
Produced by FastQC (version 0.11.5)