FastQCFastQC Report
Thu 26 May 2016
SRR1926571_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926571_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502657
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC77591.5435973238212142No Hit
GTACATGGGAAGCAGTGGTATCAAC69281.3782758421746837No Hit
GAGTACATGGGAAGCAGTGGTATCA35210.7004776617056959No Hit
CATGTACTCTGCGTTGATACCACTG29250.5819077422576429No Hit
GCGTTGATACCACTGCTTCCCATGT24030.47805959133166354No Hit
CTGTAGGACGTGGAATATGGCAAGA22670.4510033681019065No Hit
ACGCAGAGTACATGGGAAGCAGTGG22440.4464276832909917No Hit
GTCCTACAGTGGACATTTCTAAATT22310.44384142665873544No Hit
GTATCAACGCAGAGTACATGGGAAG21920.4360826567619669No Hit
ACTCTGCGTTGATACCACTGCTTCC17300.3441710749079392No Hit
CTTTAGGACGTGAAATATGGCGAGG16640.3310408489287924No Hit
GTATCAACGCAGAGTACTTTTTTTT16350.32527150721068243No Hit
GCTTCCCATGTACTCTGCGTTGATA16340.32507256439281657No Hit
GTCCTAAAGTGTGTATTTCTCATTT14070.2799125447372662No Hit
GGTATCAACGCAGAGTACATGGGAA14000.2785199450122052No Hit
TATCAACGCAGAGTACATGGGAAGC13970.2779231165586076No Hit
GTCCTACAGTGTGCATTTCTCATTT11790.23455358226384992No Hit
CAGTGGTATCAACGCAGAGTACATG10890.2166487286559224No Hit
CTGTAGGACCTGGAATATGGCGAGA10340.2057068736733001No Hit
GGTATCAACGCAGAGTACTTTTTTT10310.20511004521970252No Hit
GATATACACTGTTCTACAAATCCCG9360.1862104775224457No Hit
CATGGGAAGCAGTGGTATCAACGCA8980.178650650443543No Hit
CTGAAGGACCTGGAATATGGCGAGA8420.16750985264305482No Hit
GTGGTATCAACGCAGAGTACATGGG8360.16631619573585965No Hit
ACATGGGAAGCAGTGGTATCAACGC8320.1655204244643962No Hit
ATACCACTGCTTCCCATGTACTCTG8290.16492359601079862No Hit
GCAGTGGTATCAACGCAGAGTACAT8020.15955213992842038No Hit
CCATGTACTCTGCGTTGATACCACT7740.15398174102817627No Hit
TATCAACGCAGAGTACTTTTTTTTT7580.1507986559423225No Hit
GTGTATATCAATGAGTTACAATGAA7350.1462229711314077No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7240.14403460013488323No Hit
GGGAAGCAGTGGTATCAACGCAGAG7110.14144834350262706No Hit
CCACTGCTTCCCATGTACTCTGCGT7060.14045362941329773No Hit
CTGCTTCCCATGTACTCTGCGTTGA6790.13508217333091949No Hit
GTGTATATCAATGAGTTACAATGAG6480.1289149459770778No Hit
AAGCAGTGGTATCAACGCAGAGTAC6360.12652763216268748No Hit
GTACTCTGCGTTGATACCACTGCTT6350.1263286893448216No Hit
GATACCACTGCTTCCCATGTACTCT6250.124339261166163No Hit
TACCACTGCTTCCCATGTACTCTGC5990.11916674790165063No Hit
GTTCTACAGTGTGGTTTTTATCATT5970.1187688622659189No Hit
GAATATGGCAAGAAAACTGAAAATC5880.11697837690512616No Hit
CTTCCCATGTACTCTGCGTTGATAC5830.11598366281579686No Hit
GCAGAGTACATGGGAAGCAGTGGTA5570.11081114955128447No Hit
ACAGTGGACATTTCTAAATTTTCCA5450.10842383573689415No Hit
GATATACACTGTTCTACAATGCCGG5380.1070312360118331No Hit
ATGGGAAGCAGTGGTATCAACGCAG5340.10623546474036968No Hit
CTGTAGAACATATTAGATGAGTGAG5100.10146083711158901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAG556.072969E-717.22235318
GTCGAGG402.818551E-416.57651519
TATGTCG601.5071892E-615.78715616
TAGGACC4550.014.4653124
GAACTGT602.464988E-514.3080796
TAGGACA3050.013.4480314
TCCAACG1102.8485374E-912.91676518
ACTGTTC3500.012.7592878
ATGTCGA751.5187992E-512.62972517
AGGACAT3100.012.6157265
TTTAGAA1750.012.5391162
AGGACCT7550.012.5077265
GGACCTG6750.012.4356636
CCATCCA1001.4317084E-712.3520769
TGTAGGA12350.012.283082
CTGTAGG12150.012.2423381
GGCGAGG4050.012.16195919
GTAGGAC12300.012.0215433
TGTAGAA3700.011.8613252
GATATAC3700.011.8541981
Produced by FastQC (version 0.11.5)