##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926571_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 502657 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.335218648103975 31.0 28.0 31.0 22.0 34.0 2 28.772691915162824 31.0 28.0 33.0 23.0 34.0 3 29.094670918737826 31.0 28.0 33.0 24.0 34.0 4 32.72417771959805 35.0 33.0 37.0 26.0 37.0 5 32.41336537638986 35.0 33.0 37.0 25.0 37.0 6 32.50481143205008 35.0 33.0 37.0 25.0 37.0 7 32.35265200723356 35.0 33.0 37.0 23.0 37.0 8 32.32476022416877 35.0 33.0 37.0 23.0 37.0 9 33.515309644548864 37.0 33.0 39.0 19.0 39.0 10 33.404550220130226 37.0 33.0 39.0 19.0 39.0 11 33.615338093371825 37.0 33.0 39.0 23.0 39.0 12 33.444897813021605 37.0 33.0 39.0 19.0 39.0 13 33.56199754504563 37.0 33.0 39.0 22.0 39.0 14 34.164792293751006 38.0 33.0 40.0 17.0 41.0 15 34.302550247982225 38.0 33.0 40.0 19.0 41.0 16 34.24714666263476 38.0 33.0 40.0 19.0 41.0 17 34.203842779469895 38.0 33.0 40.0 18.0 41.0 18 34.18822377883925 38.0 33.0 40.0 18.0 41.0 19 34.2034886612541 39.0 34.0 40.0 18.0 41.0 20 34.220189910813936 39.0 34.0 40.0 18.0 41.0 21 34.12668280756062 39.0 34.0 40.0 18.0 41.0 22 34.081671995018475 39.0 34.0 40.0 17.0 41.0 23 33.754092751120545 39.0 33.0 40.0 10.0 41.0 24 33.8388423915314 39.0 34.0 40.0 2.0 41.0 25 33.63678810799412 39.0 34.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.36552276969839426 1101 2 0.6331116686535161 1101 3 0.43892600493096623 1101 4 0.14241383172871735 1101 5 0.2836482830427087 1101 6 -0.13230094443117935 1101 7 -0.09145598263959442 1101 8 0.23473709072999327 1101 9 0.5308688985058794 1101 10 0.5057311699947107 1101 11 0.23316067594190315 1101 12 0.470425371605522 1101 13 0.20923870819526513 1101 14 0.6699016350056013 1101 15 0.33862737204563587 1101 16 0.5234334656996253 1101 17 0.28138841343948684 1101 18 0.38998918090944557 1101 19 0.4991428130434059 1101 20 0.5623622310247498 1101 21 0.7177529092653288 1101 22 0.4131668159330175 1101 23 1.028809611647894 1101 24 0.7989696887438313 1101 25 0.642828652967232 1102 1 0.15079780211676663 1102 2 0.31106532032773515 1102 3 0.4330071662113646 1102 4 -0.1990424359555334 1102 5 0.1897424063677562 1102 6 -0.38084974170299546 1102 7 0.020185195718461557 1102 8 0.26761820215637044 1102 9 0.3681512180966564 1102 10 0.14699438595012282 1102 11 -0.2002828842714024 1102 12 0.2348558557463747 1102 13 0.08629949562357098 1102 14 0.3843349035463888 1102 15 0.2129674029657309 1102 16 0.16293853198160235 1102 17 0.4106972723393554 1102 18 0.11277432335139537 1102 19 0.12134217077515785 1102 20 0.0017803179563671279 1102 21 0.4065498098304303 1102 22 0.11599576626727526 1102 23 0.4474348605865117 1102 24 0.37471464831699564 1102 25 0.49519844249311973 1103 1 -0.10244166140786604 1103 2 -0.03586630799322421 1103 3 -0.18608923075493777 1103 4 -0.34682232546563796 1103 5 -0.24308120430693236 1103 6 -0.6475163160871169 1103 7 -0.03480089960849142 1103 8 -0.013710223591012038 1103 9 0.04994668582383355 1103 10 0.0632922684808932 1103 11 -0.053912149282432154 1103 12 -0.03436879089220923 1103 13 -0.07365615338344611 1103 14 -0.014194204907290953 1103 15 -0.14230702918514027 1103 16 -0.02588817384977915 1103 17 -0.10056142932823064 1103 18 -0.0650905999133613 1103 19 0.21654453948362118 1103 20 -0.10563795315776758 1103 21 0.13943335802061085 1103 22 -0.22047655796561116 1103 23 -0.01924306455111946 1103 24 -0.1539120904592579 1103 25 0.07832956169125538 1104 1 0.5149991644586933 1104 2 0.6071842175606932 1104 3 0.5520575760594397 1104 4 0.2686185929136471 1104 5 0.7145370827382891 1104 6 0.2054074942400277 1104 7 0.46072991937403174 1104 8 0.6898123345491527 1104 9 1.144857941629688 1104 10 0.9921445392602095 1104 11 0.6932165549679823 1104 12 0.7556586092008502 1104 13 0.5900396756530313 1104 14 0.9580982332656305 1104 15 0.821840207541328 1104 16 0.7689675743196887 1104 17 0.832292016906905 1104 18 0.9481904183796672 1104 19 1.0030496535327629 1104 20 0.9729271200225256 1104 21 0.9910701530532151 1104 22 0.9445362169536438 1104 23 1.2123815949884431 1104 24 0.7623720542197674 1104 25 0.9374054683840072 1105 1 0.8536541549726664 1105 2 0.771067723555813 1105 3 0.9263924747354579 1105 4 0.6574060322449782 1105 5 0.7631913875153344 1105 6 0.6705250727251553 1105 7 0.5505970133987219 1105 8 0.4278773373302016 1105 9 1.1392463277859264 1105 10 1.1983278545188725 1105 11 0.8194416747814657 1105 12 0.9333114586917546 1105 13 0.7850264307712962 1105 14 1.1653711083065872 1105 15 1.3204407962591773 1105 16 1.1417113093509457 1105 17 1.1686624875510176 1105 18 1.2271253419454027 1105 19 1.069748998527011 1105 20 1.4155322092064182 1105 21 1.1355600028768293 1105 22 1.0112279426487163 1105 23 1.6059006191086027 1105 24 1.4387270830842098 1105 25 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0.6122668982174133 1107 12 0.752226114921676 1107 13 0.6730869677964364 1107 14 1.0772343888720428 1107 15 0.9024561495703125 1107 16 0.959757048003901 1107 17 1.0010563189664055 1107 18 1.1960451094551843 1107 19 1.2463753591010374 1107 20 0.7475743358882738 1107 21 1.1678912819624472 1107 22 1.0786895350314438 1107 23 1.3711821289319914 1107 24 1.2900703761114514 1107 25 1.338101501946177 1108 1 1.0922321018746963 1108 2 1.0083126424877449 1108 3 0.8797306234121223 1108 4 0.5283137244623219 1108 5 -0.2385703234583616 1108 6 0.18475441225971423 1108 7 0.6881995596583153 1108 8 0.8177275331737377 1108 9 1.2930460447377001 1108 10 1.3339337950082708 1108 11 0.8810569288495458 1108 12 1.01733332739731 1108 13 0.96771938216569 1108 14 1.0727544389973573 1108 15 1.1386333982475563 1108 16 1.0932783512620503 1108 17 1.1227076695651235 1108 18 1.0637595344064508 1108 19 0.917720388340804 1108 20 1.0200715511486464 1108 21 1.040263183939615 1108 22 1.092815126788615 1108 23 1.3548391882469488 1108 24 1.3124840491830199 1108 25 1.3225034791113117 1109 1 0.5552229122339298 1109 2 -0.5040794488517122 1109 3 0.54043794727734 1109 4 0.6593635713016468 1109 5 -0.34918018143663687 1109 6 0.04858064450699828 1109 7 0.5980296905410825 1109 8 0.8183401758916418 1109 9 1.0418068356037509 1109 10 -1.039332704574015 1109 11 0.19662362336361383 1109 12 0.7580740681380504 1109 13 0.6761571432350308 1109 14 1.2109332192814009 1109 15 1.0782602431375636 1109 16 1.230955878413262 1109 17 1.3779569037617279 1109 18 1.3113228872329614 1109 19 1.2861414409723793 1109 20 1.4317117627888578 1109 21 1.2365316328396432 1109 22 1.1946252075460677 1109 23 1.228857567528486 1109 24 1.2277165749418586 1109 25 1.5196073498993883 1110 1 0.8947032418458392 1110 2 -0.01310390392880123 1110 3 0.5219504406319366 1110 4 0.9825826037312027 1110 5 1.300354640466601 1110 6 0.7965870240923252 1110 7 1.015015336474093 1110 8 1.1064781344243357 1110 9 1.5481266123182635 1110 10 1.3255463491208275 1110 11 1.1325420178346377 1110 12 1.3890084115723909 1110 13 1.0667273186736281 1110 14 1.5978085515514664 1110 15 1.312304634418794 1110 16 1.0321852776689724 1110 17 1.2589510558085095 1110 18 1.5968824300293463 1110 19 1.6807254388458546 1110 20 1.3652976213747152 1110 21 1.228616959793392 1110 22 1.4373617838352715 1110 23 1.719467615375379 1110 24 1.6796437413427086 1110 25 1.845976302584134 1111 1 0.4017985235216557 1111 2 0.5253290836652624 1111 3 0.5423846484822548 1111 4 0.42853033608833613 1111 5 0.6288527083941986 1111 6 0.08959088823712591 1111 7 0.6373561947209936 1111 8 0.900348888949452 1111 9 1.1231444077219663 1111 10 1.010763453045982 1111 11 0.48444104162963697 1111 12 0.6615043440515436 1111 13 0.4785002493223942 1111 14 0.7274684051207174 1111 15 0.708524382232504 1111 16 0.7601367724828876 1111 17 0.38434255468993683 1111 18 0.707135124761912 1111 19 0.6303974949776361 1111 20 0.5754104950781951 1111 21 0.7507315621932236 1111 22 0.741279314850118 1111 23 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0.6965106823766476 2202 10 0.5134008111725379 2202 11 0.47815294360611205 2202 12 0.4416877276691409 2202 13 0.3310621810589467 2202 14 0.46041280509608384 2202 15 0.5066228162369768 2202 16 0.4201022918474564 2202 17 0.4536758264507128 2202 18 0.419578426693235 2202 19 0.5113238541919713 2202 20 0.4972826088642961 2202 21 0.6045865177583565 2202 22 0.44886094980692093 2202 23 0.6832395086197494 2202 24 0.6797271441784076 2202 25 0.5415559522922209 2203 1 0.0775266684192637 2203 2 0.11556742474252957 2203 3 0.11030271335921071 2203 4 -0.3455861025624998 2203 5 -0.36315060428864143 2203 6 -0.07544094793564682 2203 7 -0.07340249569373469 2203 8 0.009930931627131656 2203 9 0.5365225913392422 2203 10 0.25205421625369695 2203 11 0.06672033951296186 2203 12 0.03157834164232298 2203 13 0.019852318673628133 2203 14 0.3588006844185969 2203 15 0.23138243162159 2203 16 0.2660576552913483 2203 17 0.2406818250392746 2203 18 0.001143793665711712 2203 19 0.08299816611857835 2203 20 0.004174369626468888 2203 21 0.1433678339192639 2203 22 0.20678923138772376 2203 23 0.411119713277472 2203 24 0.23665860148256712 2203 25 0.2020733305561606 2204 1 0.4070090365130632 2204 2 0.46916042527548285 2204 3 0.042425981257594 2204 4 0.10189291236022058 2204 5 0.17717072276766999 2204 6 0.2643174460952622 2204 7 0.29403066294900526 2204 8 0.17954920307854394 2204 9 0.631191382421143 2204 10 0.604317606731577 2204 11 0.4875100001986965 2204 12 0.4425749662648144 2204 13 0.46265687987455095 2204 14 0.9951342916312456 2204 15 0.6931124236272765 2204 16 0.501251517433829 2204 17 0.44936676019649724 2204 18 0.5939194865063584 2204 19 0.7961910067652269 2204 20 0.8065898741565789 2204 21 0.7288820238286604 2204 22 0.8360889515866887 2204 23 0.875606078528854 2204 24 0.7736128784411775 2204 25 0.817632821584759 2205 1 0.17597728517444722 2205 2 0.23367175916089522 2205 3 0.31043495166656143 2205 4 -0.09312604174358796 2205 5 -0.15285957293691865 2205 6 -0.05967942660619485 2205 7 -0.25409784433173854 2205 8 -0.09341303787774535 2205 9 0.4794968289613095 2205 10 0.35247234628624824 2205 11 0.17253240050319363 2205 12 0.22825471649241535 2205 13 0.06436094896316291 2205 14 0.6015061624038651 2205 15 0.46040900777372684 2205 16 0.07022182352845618 2205 17 0.2151871359866817 2205 18 0.32725041950089917 2205 19 0.26743580531679356 2205 20 0.30159545494426965 2205 21 0.08479482980756359 2205 22 0.24937585550970454 2205 23 0.4261263519617273 2205 24 0.27439470307585623 2205 25 0.31955394988115415 2206 1 0.16519541523536319 2206 2 0.4682376573796496 2206 3 0.5171390820126334 2206 4 0.41932251944462706 2206 5 0.45784510805641077 2206 6 0.4867937268818068 2206 7 0.3497434119306462 2206 8 0.26106121690477124 2206 9 0.5657640797073853 2206 10 0.5025556808487011 2206 11 -0.028011363662450606 2206 12 0.206565606033557 2206 13 0.13778140255883642 2206 14 0.441218645872155 2206 15 0.25220780519944697 2206 16 0.34994817150802504 2206 17 0.4873175017246254 2206 18 0.3634161446590909 2206 19 0.1030036848823741 2206 20 0.11441963622520746 2206 21 0.390871123951527 2206 22 0.220100595795941 2206 23 0.5276027749178169 2206 24 0.5582459889846945 2206 25 0.42735298329856164 2207 1 -1.0068949733717822 2207 2 -0.1403166165892742 2207 3 0.14763408534543387 2207 4 0.2753417102962814 2207 5 0.14305634175039472 2207 6 0.30056364448685713 2207 7 0.40460410591361295 2207 8 0.4644013679072714 2207 9 0.541599370902965 2207 10 0.5407130853696529 2207 11 0.3553253911777148 2207 12 0.39552782499478667 2207 13 -0.08934328982235229 2207 14 -5.03153728917497 2207 15 -1.9793128898479821 2207 16 -0.5333467649153079 2207 17 -0.3711867168665748 2207 18 -0.19293987453175987 2207 19 0.19515373443086048 2207 20 0.25691905963794426 2207 21 0.290702076169552 2207 22 0.264463599929627 2207 23 0.4975505399135258 2207 24 0.4115115004492722 2207 25 0.32343168302458736 2208 1 0.41659358152775283 2208 2 0.5833569246594585 2208 3 0.6035870006162298 2208 4 0.38794781087826635 2208 5 0.18628140268926074 2208 6 0.3429840396038131 2208 7 0.4535486174479786 2208 8 0.5761742860049353 2208 9 1.0659476411814097 2208 10 0.850759877493104 2208 11 0.5214262707307711 2208 12 0.6102458943919444 2208 13 0.3832769946995427 2208 14 1.0029405955334028 2208 15 0.8656940905350297 2208 16 0.6679672332943412 2208 17 0.5245018117480313 2208 18 0.7145954675318009 2208 19 0.5283275397686893 2208 20 0.37337242993548614 2208 21 0.7128379493869517 2208 22 0.7025812026454048 2208 23 1.169951122149378 2208 24 1.0720107156211327 2208 25 0.9953970761949691 2209 1 -0.18190551314051717 2209 2 -0.25254858165292404 2209 3 0.38422736693888737 2209 4 0.711592681413336 2209 5 0.5212815772711394 2209 6 0.7862573999058853 2209 7 1.0041923257665601 2209 8 0.671466062200949 2209 9 0.7836163246844237 2209 10 0.45189266446829635 2209 11 0.7288494921155504 2209 12 0.8836835112994521 2209 13 0.707460575024669 2209 14 1.0390141992524988 2209 15 0.8640131164679232 2209 16 0.8372792310596964 2209 17 0.9389741504967333 2209 18 1.1389541285386926 2209 19 1.046768373970771 2209 20 0.7523173568355617 2209 21 0.8374270584706949 2209 22 0.9284677044735261 2209 23 1.12502871132331 2209 24 1.1731509846767452 2209 25 1.0959238146563521 2210 1 -0.3408979225596731 2210 2 -0.48434896321733945 2210 3 0.192944458365087 2210 4 0.5281893765118895 2210 5 0.6403209902155638 2210 6 0.3915661929845342 2210 7 0.7888268950682615 2210 8 0.6703615336338231 2210 9 1.015567323780715 2210 10 1.0969129232047905 2210 11 0.8334540464640554 2210 12 0.5400299370504413 2210 13 0.4548736171460135 2210 14 0.7703315191831308 2210 15 0.9129095377410863 2210 16 1.0645950095352248 2210 17 0.9019005270188458 2210 18 0.6903513436069559 2210 19 0.8837472848024817 2210 20 0.989069120146219 2210 21 0.9427059994259039 2210 22 0.9426189678968058 2210 23 0.755581545511042 2210 24 0.7726386136854089 2210 25 0.7886640496197046 2211 1 -0.9684185420306619 2211 2 -0.08145724869724091 2211 3 0.4142531850573228 2211 4 0.3720980411239978 2211 5 0.7995398891801528 2211 6 0.6550561493067306 2211 7 0.6875282009680888 2211 8 0.6262680143557233 2211 9 0.896668636331988 2211 10 0.923702438314649 2211 11 0.5234511087437284 2211 12 0.8450761645741025 2211 13 0.7377178847113655 2211 14 0.6659260472633051 2211 15 0.4523775332181117 2211 16 0.5464005435351851 2211 17 0.6282906784500142 2211 18 0.6223112464958973 2211 19 0.9969132604582001 2211 20 0.7289339850110821 2211 21 0.49557665104553195 2211 22 0.50706590047335 2211 23 1.0079538932484482 2211 24 0.716543398289538 2211 25 0.7141152391192946 2212 1 1.360457506861028 2212 2 0.3316787555710832 2212 3 0.6946134460070255 2212 4 0.7061336670637601 2212 5 0.9300753696089288 2212 6 0.830333226476057 2212 7 1.0939706998767562 2212 8 0.9807449023607688 2212 9 1.2026691456204404 2212 10 0.9236387772582404 2212 11 0.9108225740393578 2212 12 1.0197135997004594 2212 13 1.0903455706016487 2212 14 0.8988163804834599 2212 15 0.8864428676508567 2212 16 -0.07307003899379794 2212 17 0.7251464285591496 2212 18 0.6132501561335815 2212 19 0.7939557496661394 2212 20 0.884782314059926 2212 21 0.9272410542079683 2212 22 0.9972560348284958 2212 23 1.1933955190911973 2212 24 1.0269153001181266 2212 25 0.7205965409575867 2213 1 0.07781187050531457 2213 2 -0.25975852049867143 2213 3 -0.6417955591310118 2213 4 -1.1318271390683705 2213 5 -2.4033640984216262 2213 6 -1.1047760196436016 2213 7 -0.5765397186402836 2213 8 -0.32436665637808204 2213 9 -0.8816040383869606 2213 10 -0.7097490757932405 2213 11 -0.6355286826513193 2213 12 -0.8628154002090511 2213 13 -0.79564230322989 2213 14 -8.547795632400174 2213 15 -3.58236023461048 2213 16 -2.0449348479681007 2213 17 -1.12885859217063 2213 18 -4.9100972428401555 2213 19 -2.6025461624342263 2213 20 -4.3950881792653185 2213 21 -1.7139292651680904 2213 22 -1.3439156251963738 2213 23 -4.319608774336608 2213 24 -2.2481238300689306 2213 25 -1.558255059274348 2214 1 0.5981889415394015 2214 2 0.28281029423258985 2214 3 -1.2732998147306773 2214 4 -1.5611354759419314 2214 5 -0.010217371790801622 2214 6 -0.1561453558872472 2214 7 -0.026378943403329913 2214 8 -0.0629677287013024 2214 9 -4.811130282472337 2214 10 -0.7615450706953126 2214 11 -0.2272511384472864 2214 12 -0.6283179418494669 2214 13 -0.9859412206522151 2214 14 -2.2448753095312703 2214 15 -1.5018041500551575 2214 16 -1.045713088010288 2214 17 -1.6050938880117158 2214 18 -1.0966441276826018 2214 19 -4.253378238375802 2214 20 -1.960672756561955 2214 21 -3.6088064314221384 2214 22 -6.008463037410895 2214 23 -6.563410321294697 2214 24 -5.681975257635841 2214 25 -3.567711133575216 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18751.0 3 15.0 4 357.0 5 2128.0 6 2734.0 7 2194.0 8 1632.0 9 1392.0 10 1245.0 11 1141.0 12 1004.0 13 988.0 14 1016.0 15 1000.0 16 1029.0 17 1098.0 18 1252.0 19 1481.0 20 1882.0 21 2489.0 22 3068.0 23 3809.0 24 4514.0 25 5390.0 26 5983.0 27 6731.0 28 7752.0 29 9268.0 30 11473.0 31 14255.0 32 18267.0 33 24110.0 34 30975.0 35 41328.0 36 56158.0 37 83470.0 38 131278.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.424849811131395 20.70664932965088 9.732557282840935 29.13594357637679 2 11.0424771892817 22.249529855424324 42.06545208505388 24.642540870240097 3 19.5443337253942 26.89040008119839 28.813488485948497 24.751777707458906 4 11.678803063984255 19.45717566564441 37.869340853322406 30.994680417048937 5 11.616971655536364 40.20082303216512 35.751753122189236 12.430452190109287 6 30.51814868440761 37.78780767886564 18.54730640571172 13.14673723101503 7 25.582058253665775 32.74117543873638 22.220472503727564 19.456293803870288 8 23.460538592196233 41.196043093755236 20.39656781046403 14.946850503584502 9 25.77525948314705 16.77279922803016 20.59955260838879 36.852388680434 10 15.421900587180717 31.419839186900955 33.589482879036 19.56877734688233 11 31.09158768432529 24.28418522851163 24.80423520423998 19.8199918829231 12 24.382703154376436 26.97941846469108 31.600465546569577 17.037412834362907 13 29.600502927443568 23.387518725492733 25.133639837901395 21.87833850916231 14 19.684456160750553 26.650592142464617 26.971352569667335 26.693599127117494 15 24.152879572818932 32.75897056365937 22.855340957019283 20.232808906502413 16 18.436434389212287 29.760109500523228 33.22915133115553 18.574304779108957 17 20.43486737917698 31.536937266729804 29.670377768404972 18.357817585688252 18 20.467251045475706 28.2371946633986 33.3650988178465 17.930455473279192 19 22.89934189061219 28.62676364562361 28.544002610155733 19.92989185360846 20 22.551785330606464 29.527386740727184 29.324065158399847 18.596762770266505 21 19.721599420678515 28.88888446793738 28.739677354537985 22.64983875684612 22 21.161149650755885 31.86705845138932 26.043803229637707 20.927988668217097 23 18.79949945987025 31.118635570577947 29.013422671921408 21.06844229763039 24 22.11726883341921 30.330623069011274 29.083251600992327 18.46885649657719 25 20.913664785330752 29.158253043327758 28.391129537636996 21.536952633704495 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 685.0 1 685.0 2 670.0 3 655.0 4 655.0 5 655.0 6 690.5 7 726.0 8 726.0 9 726.0 10 1069.5 11 1413.0 12 1413.0 13 1413.0 14 2022.5 15 2632.0 16 2632.0 17 2632.0 18 4391.0 19 6150.0 20 6150.0 21 6150.0 22 10045.5 23 13941.0 24 13941.0 25 13941.0 26 20698.0 27 27455.0 28 27455.0 29 27455.0 30 37708.0 31 47961.0 32 47961.0 33 47961.0 34 52191.5 35 56422.0 36 56422.0 37 56422.0 38 57901.5 39 59381.0 40 59381.0 41 59381.0 42 61373.5 43 63366.0 44 63366.0 45 63366.0 46 75677.5 47 87989.0 48 87989.0 49 87989.0 50 77212.5 51 66436.0 52 66436.0 53 66436.0 54 49893.0 55 33350.0 56 33350.0 57 33350.0 58 25465.0 59 17580.0 60 17580.0 61 17580.0 62 13532.0 63 9484.0 64 9484.0 65 9484.0 66 6954.0 67 4424.0 68 4424.0 69 4424.0 70 3064.0 71 1704.0 72 1704.0 73 1704.0 74 1162.5 75 621.0 76 621.0 77 621.0 78 412.0 79 203.0 80 203.0 81 203.0 82 130.0 83 57.0 84 57.0 85 57.0 86 37.0 87 17.0 88 17.0 89 17.0 90 11.0 91 5.0 92 5.0 93 5.0 94 2.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09071792494683252 2 0.030239308315610842 3 0.03660547848731839 4 0.03461605030865978 5 0.0248678522332326 6 0.3843575241168431 7 0.19675444686933635 8 0.20948678721275146 9 0.21545507174872724 10 0.017109082336464028 11 0.0025862566322561904 12 0.004376741993048938 13 0.0 14 0.08216338377860052 15 0.0025862566322561904 16 0.002188370996524469 17 0.004177799175183077 18 0.002984142267987912 19 9.947140893293042E-4 20 9.947140893293042E-4 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 502657.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.5695670907159 #Duplication Level Percentage of deduplicated Percentage of total 1 92.57040204300621 63.475123935024605 2 5.217926602225434 7.155819364514564 3 0.9331750571572628 1.9196222906738274 4 0.34501250880168055 0.9462943347765219 5 0.19461592870944763 0.6672364990282226 6 0.12799563219073418 0.5265963053292686 7 0.09270676578157953 0.4449803957216313 8 0.06883903909842504 0.3776210487935897 9 0.055206488633933126 0.34069365236045906 >10 0.3071033102177376 4.080424031574441 >50 0.038867425789630275 1.8836847518478208 >100 0.03451659402507996 4.9901435844974955 >500 0.007831496123337466 3.710685885052601 >1k 0.005220997415558311 6.559980304801948 >5k 5.801108239509233E-4 2.9210936160029775 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 7759 1.5435973238212142 No Hit GTACATGGGAAGCAGTGGTATCAAC 6928 1.3782758421746837 No Hit GAGTACATGGGAAGCAGTGGTATCA 3521 0.7004776617056959 No Hit CATGTACTCTGCGTTGATACCACTG 2925 0.5819077422576429 No Hit GCGTTGATACCACTGCTTCCCATGT 2403 0.47805959133166354 No Hit CTGTAGGACGTGGAATATGGCAAGA 2267 0.4510033681019065 No Hit ACGCAGAGTACATGGGAAGCAGTGG 2244 0.4464276832909917 No Hit GTCCTACAGTGGACATTTCTAAATT 2231 0.44384142665873544 No Hit GTATCAACGCAGAGTACATGGGAAG 2192 0.4360826567619669 No Hit ACTCTGCGTTGATACCACTGCTTCC 1730 0.3441710749079392 No Hit CTTTAGGACGTGAAATATGGCGAGG 1664 0.3310408489287924 No Hit GTATCAACGCAGAGTACTTTTTTTT 1635 0.32527150721068243 No Hit GCTTCCCATGTACTCTGCGTTGATA 1634 0.32507256439281657 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1407 0.2799125447372662 No Hit GGTATCAACGCAGAGTACATGGGAA 1400 0.2785199450122052 No Hit TATCAACGCAGAGTACATGGGAAGC 1397 0.2779231165586076 No Hit GTCCTACAGTGTGCATTTCTCATTT 1179 0.23455358226384992 No Hit CAGTGGTATCAACGCAGAGTACATG 1089 0.2166487286559224 No Hit CTGTAGGACCTGGAATATGGCGAGA 1034 0.2057068736733001 No Hit GGTATCAACGCAGAGTACTTTTTTT 1031 0.20511004521970252 No Hit GATATACACTGTTCTACAAATCCCG 936 0.1862104775224457 No Hit CATGGGAAGCAGTGGTATCAACGCA 898 0.178650650443543 No Hit CTGAAGGACCTGGAATATGGCGAGA 842 0.16750985264305482 No Hit GTGGTATCAACGCAGAGTACATGGG 836 0.16631619573585965 No Hit ACATGGGAAGCAGTGGTATCAACGC 832 0.1655204244643962 No Hit ATACCACTGCTTCCCATGTACTCTG 829 0.16492359601079862 No Hit GCAGTGGTATCAACGCAGAGTACAT 802 0.15955213992842038 No Hit CCATGTACTCTGCGTTGATACCACT 774 0.15398174102817627 No Hit TATCAACGCAGAGTACTTTTTTTTT 758 0.1507986559423225 No Hit GTGTATATCAATGAGTTACAATGAA 735 0.1462229711314077 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 724 0.14403460013488323 No Hit GGGAAGCAGTGGTATCAACGCAGAG 711 0.14144834350262706 No Hit CCACTGCTTCCCATGTACTCTGCGT 706 0.14045362941329773 No Hit CTGCTTCCCATGTACTCTGCGTTGA 679 0.13508217333091949 No Hit GTGTATATCAATGAGTTACAATGAG 648 0.1289149459770778 No Hit AAGCAGTGGTATCAACGCAGAGTAC 636 0.12652763216268748 No Hit GTACTCTGCGTTGATACCACTGCTT 635 0.1263286893448216 No Hit GATACCACTGCTTCCCATGTACTCT 625 0.124339261166163 No Hit TACCACTGCTTCCCATGTACTCTGC 599 0.11916674790165063 No Hit GTTCTACAGTGTGGTTTTTATCATT 597 0.1187688622659189 No Hit GAATATGGCAAGAAAACTGAAAATC 588 0.11697837690512616 No Hit CTTCCCATGTACTCTGCGTTGATAC 583 0.11598366281579686 No Hit GCAGAGTACATGGGAAGCAGTGGTA 557 0.11081114955128447 No Hit ACAGTGGACATTTCTAAATTTTCCA 545 0.10842383573689415 No Hit GATATACACTGTTCTACAATGCCGG 538 0.1070312360118331 No Hit ATGGGAAGCAGTGGTATCAACGCAG 534 0.10623546474036968 No Hit CTGTAGAACATATTAGATGAGTGAG 510 0.10146083711158901 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTCGAG 55 6.072969E-7 17.222353 18 GTCGAGG 40 2.818551E-4 16.576515 19 TATGTCG 60 1.5071892E-6 15.787156 16 TAGGACC 455 0.0 14.465312 4 GAACTGT 60 2.464988E-5 14.308079 6 TAGGACA 305 0.0 13.448031 4 TCCAACG 110 2.8485374E-9 12.916765 18 ACTGTTC 350 0.0 12.759287 8 ATGTCGA 75 1.5187992E-5 12.629725 17 AGGACAT 310 0.0 12.615726 5 TTTAGAA 175 0.0 12.539116 2 AGGACCT 755 0.0 12.507726 5 GGACCTG 675 0.0 12.435663 6 CCATCCA 100 1.4317084E-7 12.352076 9 TGTAGGA 1235 0.0 12.28308 2 CTGTAGG 1215 0.0 12.242338 1 GGCGAGG 405 0.0 12.161959 19 GTAGGAC 1230 0.0 12.021543 3 TGTAGAA 370 0.0 11.861325 2 GATATAC 370 0.0 11.854198 1 >>END_MODULE