FastQCFastQC Report
Thu 26 May 2016
SRR1926570_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926570_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640412
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC17640.2754476805556423No Hit
CCCATGTACTCTGCGTTGATACCAC16100.25140066082459417No Hit
GTCCTACAGTGGACATTTCTAAATT13590.21220714165256113No Hit
CTGTAGGACGTGGAATATGGCAAGA10410.16255160740273447No Hit
GTCCTACAGTGTGCATTTCTCATTT8690.13569389705377163No Hit
GAGTACATGGGAAGCAGTGGTATCA8090.12632492832738923No Hit
GTCCTAAAGTGTGTATTTCTCATTT7430.11601906272836861No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG501.1022683E-418.7390041
CCTAGAC300.007573487718.2618983
TGTAGGA6950.017.1844652
CTACTCT452.6815833E-416.6845154
CTGTAGG6950.016.5627371
TAGGACC2950.015.9977454
TGTAGAA3250.015.6285932
GTCCTAC9150.015.5061241
GACTGCA400.003302222415.289117
CCTTATC550.0037875314.9758022
TCCTACC550.0037875314.9758022
GTAGGAC7800.014.7499933
GGAGTGT652.2610826E-514.4391896
TCCTACA10150.014.3364432
AGGACCT5350.014.0418985
TAGGACA2450.014.0091554
TATGCTG601.8465413E-413.911885
TTGTAGA1451.0040822E-913.8465541
TACTATA600.00674731813.72781852
GTTCTAG600.00797660613.3850021
Produced by FastQC (version 0.11.5)