FastQCFastQC Report
Thu 26 May 2016
SRR1926570_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926570_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640412
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC47280.7382747356389324No Hit
GTACATGGGAAGCAGTGGTATCAAC42830.6687882175849297No Hit
CTGTAGGACGTGGAATATGGCAAGA30070.46954148267053086No Hit
GTCCTACAGTGGACATTTCTAAATT27830.43456399942536994No Hit
GAGTACATGGGAAGCAGTGGTATCA19240.30043159715932866No Hit
GTCCTACAGTGTGCATTTCTCATTT18370.2868465925060742No Hit
CTGTAGGACCTGGAATATGGCGAGA16210.2531183050910976No Hit
CATGTACTCTGCGTTGATACCACTG16210.2531183050910976No Hit
CTTTAGGACGTGAAATATGGCGAGG16110.2515568103033672No Hit
GTCCTAAAGTGTGTATTTCTCATTT15970.24937071760054463No Hit
GTATCAACGCAGAGTACTTTTTTTT14760.2304766306690068No Hit
GCGTTGATACCACTGCTTCCCATGT13230.2065857604167317No Hit
ACGCAGAGTACATGGGAAGCAGTGG13150.20533656458654742No Hit
GATATACACTGTTCTACAAATCCCG12680.19799753908421452No Hit
GTATCAACGCAGAGTACATGGGAAG11410.17816655528003847No Hit
GGTATCAACGCAGAGTACTTTTTTT9770.15255804076125995No Hit
CTGAAGGACCTGGAATATGGCGAGA9700.15146499440984865No Hit
ACTCTGCGTTGATACCACTGCTTCC9350.14599976265279227No Hit
GTGTATATCAATGAGTTACAATGAA9180.1433452215136506No Hit
GCTTCCCATGTACTCTGCGTTGATA9120.14240832464101236No Hit
GTGTATATCAATGAGTTACAATGAG8680.1355377475749986No Hit
CTGTAGAACATATTAGATGAGTGAG8180.1277302736363466No Hit
GTTCTACAGTGTGGTTTTTATCATT7870.12288963979438236No Hit
GATATACACTGTTCTACAATGCCGG7520.11742440803732598No Hit
TATCAACGCAGAGTACATGGGAAGC7160.11180302680149654No Hit
GGTATCAACGCAGAGTACATGGGAA7080.11055383097131222No Hit
TATCAACGCAGAGTACTTTTTTTTT7010.10946078461990094No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6920.1080554393109436No Hit
CTGTAGGACATGGAATATGGCAAGA6910.10789928983217054No Hit
CAGTGGTATCAACGCAGAGTACATG6410.10009181589351855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5500.015.7779984
CCAACGT851.0568328E-915.60359319
GCCGGTT1250.015.17080711
TCCAACG2200.014.64217718
CCGGTTT1250.014.41339212
CCAACGA1400.014.21041619
GTTCTAG759.291707E-713.9797651
GTAGGAC14300.013.2705893
AGGACCT8700.013.0437025
TGTAGGA14950.013.0125432
ACTGTTC4550.012.94852358
GGCGAGG4550.012.90909619
CTGTAGG14700.012.90341
GGACCTG8650.012.898616
TGCCGGT1403.6379788E-1212.869110
GATATAC4900.012.6440351
TCTAAAT8200.012.59395318
CTAAATT7850.012.55102519
CACTGTT5100.012.2974127
ATACACT5550.012.1994024
Produced by FastQC (version 0.11.5)