FastQCFastQC Report
Thu 26 May 2016
SRR1926569_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926569_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538059
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC13010.2417950447813344No Hit
CCCATGTACTCTGCGTTGATACCAC12750.2369628609501932No Hit
GTCCTACAGTGGACATTTCTAAATT8910.16559522282872324No Hit
CTGTAGGACGTGGAATATGGCAAGA6430.11950362320860723No Hit
GAGTACATGGGAAGCAGTGGTATCA6380.11857435708723393No Hit
GTCCTACAGTGTGCATTTCTCATTT5420.10073244755686644No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT250.00346617120.9045226
CTGTAGG5050.017.5092011
TGTAGGA5500.017.251862
TATTAGG500.001982124816.501782
GTGTAGC500.002359860316.0766321
GTGTAAG500.002359860316.0766321
GTCCTAA1950.015.8018171
GAACAGT951.2623786E-915.4033336
GTCCTAT708.154482E-515.3110771
GTAGGAC6000.014.8470723
GTCCTAC5800.014.5521231
TAGGACC2300.014.5103724
TAGCCCT450.005020270614.3543464
GTCTTAG751.4674816E-414.29033851
TAGGACA1600.014.1300594
TTTAGGA2150.014.0712852
CCTAGAC550.001017159713.997243
AATAGTG450.006086729513.9435345
GCCGGTT400.006523405713.79904811
CGTTCAG400.006523405713.79904810
Produced by FastQC (version 0.11.5)