##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926569_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 538059 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.944119511057337 26.0 2.0 30.0 2.0 31.0 2 15.46839287141373 12.0 2.0 30.0 2.0 31.0 3 18.788183080294168 16.0 7.0 30.0 2.0 31.0 4 20.773312963819954 27.0 2.0 35.0 2.0 35.0 5 20.98267104536863 27.0 2.0 35.0 2.0 35.0 6 21.927173414067973 29.0 2.0 35.0 2.0 36.0 7 21.61793037566512 28.0 2.0 35.0 2.0 37.0 8 21.855090241033047 29.0 2.0 35.0 2.0 37.0 9 24.538485556416674 31.0 10.0 37.0 2.0 39.0 10 25.185790034178407 31.0 8.0 37.0 2.0 39.0 11 22.468430042058586 29.0 2.0 35.0 2.0 39.0 12 23.543661568712725 30.0 2.0 37.0 2.0 39.0 13 20.358254392176324 25.0 2.0 35.0 2.0 39.0 14 23.5114086001721 29.0 2.0 37.0 2.0 40.0 15 24.552108597756007 30.0 2.0 38.0 2.0 40.0 16 24.88695663486718 31.0 2.0 38.0 2.0 40.0 17 24.227131225386064 30.0 2.0 38.0 2.0 40.0 18 24.297017613310064 30.0 2.0 37.0 2.0 40.0 19 24.643171102053863 30.0 2.0 38.0 2.0 40.0 20 24.89565084869875 31.0 2.0 38.0 2.0 40.0 21 22.832981141473333 30.0 2.0 36.0 2.0 40.0 22 23.70330948836466 30.0 2.0 37.0 2.0 40.0 23 23.659533619919006 30.0 2.0 37.0 2.0 40.0 24 22.824342683609046 30.0 2.0 38.0 2.0 40.0 25 22.605680789653178 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.525404628205052 1101 2 10.046156331152241 1101 3 6.842142612391587 1101 4 8.37980385411209 1101 5 8.043732326200182 1101 6 7.3944542604481 1101 7 7.116496854348206 1101 8 5.67702196533579 1101 9 5.6819874334129565 1101 10 5.192730403335126 1101 11 8.054430519886115 1101 12 5.581061130359043 1101 13 10.027507623032069 1101 14 7.584298015147656 1101 15 6.798218268783835 1101 16 6.310345554747613 1101 17 2.1270200765694263 1101 18 4.574541144107318 1101 19 5.428696397210018 1101 20 3.6256686015283393 1101 21 0.9897851153740866 1101 22 4.322138475194507 1101 23 5.365426393886832 1101 24 6.323084506197269 1101 25 6.921707959018185 1102 1 4.853800633071646 1102 2 9.443660787480228 1102 3 6.175122268900655 1102 4 7.437361220492985 1102 5 6.9715185922217415 1102 6 6.036186711004081 1102 7 5.794997547554779 1102 8 6.037270104995695 1102 9 5.2016164971596694 1102 10 4.778016032738403 1102 11 7.11183934200082 1102 12 4.684999675474057 1102 13 8.570486430716759 1102 14 6.589942697262927 1102 15 4.671736362890165 1102 16 4.907153975085446 1102 17 5.767373187510774 1102 18 5.648940600859717 1102 19 4.539128448818538 1102 20 4.9756176720656455 1102 21 3.8045362683605326 1102 22 4.881138843018405 1102 23 5.532439663841281 1102 24 6.785835263065675 1102 25 6.670102266236025 1103 1 4.515094327818414 1103 2 8.60783638138191 1103 3 5.760304603072662 1103 4 7.3128943005613145 1103 5 6.653684079281707 1103 6 6.200301720875714 1103 7 6.102510204865371 1103 8 5.861128571198119 1103 9 5.0493331919800895 1103 10 4.3697652005989305 1103 11 5.0367840442243725 1103 12 3.8516734696736705 1103 13 7.526268404235598 1103 14 5.986564959399335 1103 15 5.3389720442704345 1103 16 4.709126163451888 1103 17 2.6371816707004854 1103 18 1.5318459258395833 1103 19 3.652367128514335 1103 20 4.007851939818547 1103 21 5.787420685910419 1103 22 1.9184918673494273 1103 23 4.093930078497387 1103 24 5.321564397806931 1103 25 1.3952051996909987 1104 1 5.499599814126622 1104 2 10.130254643263582 1104 3 6.783334428176236 1104 4 7.336827557878465 1104 5 7.478969998408537 1104 6 6.982462072397283 1104 7 6.698398937064621 1104 8 6.6795777700088586 1104 9 5.68254089732147 1104 10 4.733605651865098 1104 11 7.605299845999742 1104 12 4.782155367283899 1104 13 8.782911827947672 1104 14 6.597426038047669 1104 15 6.196638252708706 1104 16 5.474770163797629 1104 17 6.0476737985260804 1104 18 3.5893612859632924 1104 19 4.497304770378651 1104 20 3.7403554890165296 1104 21 3.0228695662109786 1104 22 4.9259182459123885 1104 23 5.42423105285436 1104 24 6.523304481661544 1104 25 5.617161799423169 1105 1 3.522832388819033 1105 2 9.273543224846991 1105 3 6.386387925743467 1105 4 5.568144666511028 1105 5 1.9026237935415935 1105 6 4.949611786811225 1105 7 5.5755355730747596 1105 8 2.989627103290765 1105 9 3.7474525567359933 1105 10 3.44650488504287 1105 11 6.273392397620867 1105 12 3.870964624783525 1105 13 4.729826832348049 1105 14 3.703259426897013 1105 15 3.9500933940174114 1105 16 4.407161010551228 1105 17 5.044275049798564 1105 18 2.785790963537977 1105 19 2.367719878214121 1105 20 1.6051687389940312 1105 21 2.577028066180006 1105 22 3.5907406122035326 1105 23 1.40353351460967 1105 24 4.126093839884753 1105 25 4.654910350455239 1106 1 6.517752820553245 1106 2 10.76491144990736 1106 3 7.75319210344108 1106 4 9.058932203240438 1106 5 8.82144263391049 1106 6 7.8238037397975795 1106 7 7.51399890185025 1106 8 7.321218759532584 1106 9 6.368650590076115 1106 10 5.791596672201393 1106 11 8.139709565165159 1106 12 5.647292556590468 1106 13 4.571389686914131 1106 14 5.917598588448232 1106 15 6.187427347992912 1106 16 6.226224810626871 1106 17 6.6319130214623705 1106 18 6.705838675404852 1106 19 6.780340248853868 1106 20 6.317334683194421 1106 21 6.560065915654885 1106 22 6.626253369309403 1106 23 7.006810505270945 1106 24 7.871407894520658 1106 25 7.080442017752247 1107 1 3.5183158836163066 1107 2 -13.10535882036291 1107 3 -5.148536500162525 1107 4 1.8433184645016993 1107 5 4.377850291568148 1107 6 4.6364726587164995 1107 7 4.457974127075477 1107 8 5.179889930703755 1107 9 3.622490680166205 1107 10 3.0461124379671602 1107 11 5.616714069669666 1107 12 2.8263803944283055 1107 13 4.502791713941157 1107 14 3.9727449848446277 1107 15 3.8103665371821016 1107 16 3.6194905763926357 1107 17 4.126952435876785 1107 18 -0.6075340723428972 1107 19 2.005509167772786 1107 20 2.0115858093205468 1107 21 -0.9265162915523213 1107 22 2.1794571981382305 1107 23 -0.7646872424768034 1107 24 2.2868020386648027 1107 25 2.988212288022517 1108 1 6.1343301494479014 1108 2 11.082532620138133 1108 3 1.5274312277428344 1108 4 6.688349344807893 1108 5 8.159587417525003 1108 6 8.066731009447189 1108 7 8.316329207103312 1108 8 8.111248664176266 1108 9 6.649165175642892 1108 10 5.853841213193327 1108 11 8.072202935362078 1108 12 4.014232690879247 1108 13 4.68522255945264 1108 14 6.305908693960841 1108 15 6.261292946367906 1108 16 5.850431773330701 1108 17 1.427524373942198 1108 18 4.789893293697464 1108 19 6.144979418294707 1108 20 6.323892263739477 1108 21 6.053805124592426 1108 22 3.3121316066281388 1108 23 6.06952900665333 1108 24 6.906352317022492 1108 25 7.204783269233371 1109 1 3.8368730694703324 1109 2 -13.10535882036291 1109 3 -4.963037534775015 1109 4 2.52838007097273 1109 5 0.8411168453679956 1109 6 2.7402395887390725 1109 7 4.187380635728601 1109 8 4.313006935970872 1109 9 3.994391545478468 1109 10 3.26818260350516 1109 11 1.2896918725289588 1109 12 2.0370039835930918 1109 13 3.2888456078463513 1109 14 4.073558566484959 1109 15 4.094268907385263 1109 16 4.067506001524315 1109 17 4.526583267329006 1109 18 4.674870461140927 1109 19 3.2184465266892666 1109 20 2.363746738590674 1109 21 1.394559235159491 1109 22 3.577625463751474 1109 23 4.12428472893027 1109 24 5.119284168085418 1109 25 5.2033082248171 1110 1 3.242731419061254 1110 2 9.019075568777362 1110 3 6.290297398781668 1110 4 7.514703268423268 1110 5 8.121468315700877 1110 6 3.7734918894857294 1110 7 6.215393056185583 1110 8 7.033038497822911 1110 9 5.672330423755948 1110 10 5.134979330878171 1110 11 2.7531582627315068 1110 12 5.728614557324235 1110 13 9.897828478647043 1110 14 7.748188046684749 1110 15 7.025892555281651 1110 16 6.560874437629437 1110 17 7.048921704353408 1110 18 7.194440852242323 1110 19 5.796544235646092 1110 20 6.141907264825825 1110 21 3.9316520719469246 1110 22 6.043573902512289 1110 23 6.385548428714749 1110 24 7.532399700806582 1110 25 7.966775636438804 1111 1 4.906741809542233 1111 2 9.041731126727035 1111 3 6.9131863834353595 1111 4 7.6285532528614866 1111 5 7.856454788922644 1111 6 7.253833769827612 1111 7 6.973086560937912 1111 8 6.949532787846611 1111 9 5.802021103446627 1111 10 5.07736243796716 1111 11 6.831727297182894 1111 12 6.055712405010318 1111 13 8.314183909708355 1111 14 6.047116677966319 1111 15 5.407092727658295 1111 16 5.300773645175703 1111 17 6.494793044342394 1111 18 6.0945988641650395 1111 19 5.436825305338925 1111 20 4.964942290802028 1111 21 7.321747597336568 1111 22 6.873918176974211 1111 23 6.736734715195155 1111 24 6.457668440781205 1111 25 6.9687017060119345 1112 1 -1.794487471130303 1112 2 6.997424905547154 1112 3 5.369131683277875 1112 4 -0.2755096047345589 1112 5 3.659171669155583 1112 6 2.6358704103224966 1112 7 5.311244112799958 1112 8 5.0408553125738464 1112 9 3.925707132700108 1112 10 3.0294680767965687 1112 11 -0.3697331109156323 1112 12 3.551768510059997 1112 13 7.186939197881202 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4.830890658505794 2204 9 3.141258875217151 2204 10 2.9235050871665784 2204 11 4.344752643177671 2204 12 4.175916420629179 2204 13 2.6795520851201964 2204 14 3.7825248247281777 2204 15 3.852642738055465 2204 16 3.6951637932776435 2204 17 -0.944167469508951 2204 18 2.4013951489089216 2204 19 2.8608312201745356 2204 20 3.315265758374405 2204 21 4.5704860738043 2204 22 4.180817278196454 2204 23 3.0856857560675905 2204 24 4.658681587427537 2204 25 4.770362943044351 2205 1 5.973244905698326 2205 2 10.184861999826365 2205 3 7.211712579753353 2205 4 7.7764710670253585 2205 5 7.58349566485942 2205 6 6.993826759662873 2205 7 7.218088480387781 2205 8 6.792495083174838 2205 9 5.5572895760652585 2205 10 5.064113226610694 2205 11 6.9890721138337035 2205 12 6.905067302945657 2205 13 9.365525020986375 2205 14 7.067139043730013 2205 15 6.043745874721534 2205 16 5.596449041166558 2205 17 6.495791158274262 2205 18 6.350730254681288 2205 19 5.036798599949442 2205 20 5.5534923550618736 2205 21 -0.5477932368730372 2205 22 4.044819316960524 2205 23 5.121018593485342 2205 24 6.839779168002341 2205 25 7.321690206003588 2206 1 3.680401804941358 2206 2 7.7673119870905065 2206 3 5.449050197560041 2206 4 1.5838787647087393 2206 5 5.09346882158885 2206 6 5.486822037598486 2206 7 5.5181061146531185 2206 8 5.284729475217212 2206 9 4.232570907909476 2206 10 3.27342869055515 2206 11 2.947899369876705 2206 12 4.187780497947976 2206 13 6.815104084541574 2206 14 -0.17685387643901507 2206 15 1.8207573239315842 2206 16 2.752280431465099 2206 17 3.775147311653182 2206 18 -1.2992589688232279 2206 19 0.4839382567997035 2206 20 1.9633779935855564 2206 21 2.6289249610356684 2206 22 -2.6914602449259526 2206 23 -3.416382377052372 2206 24 0.20234292893395178 2206 25 1.6668613563454961 2207 1 1.2706929624304806 2207 2 -13.10535882036291 2207 3 -5.786318947740254 2207 4 -3.6640003171324302 2207 5 1.2359621860005419 2207 6 1.8084493398444828 2207 7 2.78202962237555 2207 8 3.2033082242684685 2207 9 0.9104832687417641 2207 10 0.5941375645036757 2207 11 -1.4171163714004713 2207 12 -2.27634465980163 2207 13 0.15420847099105117 2207 14 -5.093148905249372 2207 15 -1.5000862827745145 2207 16 -0.27357703025957036 2207 17 0.5553802196801136 2207 18 0.5995949045189981 2207 19 0.07453031744740812 2207 20 -0.19600412198820294 2207 21 -3.460344473041836 2207 22 -3.8629076789405836 2207 23 -1.2957022460921337 2207 24 0.18273931270674026 2207 25 0.7778272554845564 2208 1 4.7900751428755655 2208 2 6.354517722846966 2208 3 6.206954754981506 2208 4 7.7041852340490244 2208 5 3.7786476166710266 2208 6 5.868854345959299 2208 7 6.565150053001403 2208 8 6.61152573317289 2208 9 5.0840904744048885 2208 10 4.7664570881729205 2208 11 6.604214069669666 2208 12 6.145541917062051 2208 13 8.364301487603711 2208 14 3.341032021881663 2208 15 4.664353162696507 2208 16 5.14269016487 2208 17 5.845380933819172 2208 18 1.308708211607719 2208 19 3.5583126657151745 2208 20 4.623584266110672 2208 21 7.095204387460026 2208 22 5.716892897726709 2208 23 2.0377047328318376 2208 24 3.1621364008047195 2208 25 4.509971547281776 2209 1 2.274669062400992 2209 2 3.9146622544526846 2209 3 4.326883898502736 2209 4 0.6262138368234424 2209 5 4.095946094167374 2209 6 4.607000846282357 2209 7 5.254784365923388 2209 8 3.7013116026391266 2209 9 3.525070149783346 2209 10 2.6045138253925195 2209 11 -1.082473671812597 2209 12 2.2149934081269365 2209 13 1.4724736159432652 2209 14 2.898919466755409 2209 15 3.0284184339155082 2209 16 2.580849023881221 2209 17 0.41432181592595896 2209 18 2.529499427832725 2209 19 3.0354024765188363 2209 20 3.2524105810100465 2209 21 5.221337809255548 2209 22 4.195328070986843 2209 23 -0.05184697201666921 2209 24 -2.9740932019393433 2209 25 -0.20186937688791673 2210 1 -3.660243238618005 2210 2 -13.10535882036291 2210 3 -7.977883389897745 2210 4 -3.615809437203051 2210 5 -2.4773886456824883 2210 6 -3.261412270554448 2210 7 -3.065708771002143 2210 8 -19.44094340262958 2210 9 -13.174179160240033 2210 10 -8.22485011190589 2210 11 -10.30075509971735 2210 12 -6.171191694799116 2210 13 -6.606284047756338 2210 14 -5.45761183416154 2210 15 -7.191000437790695 2210 16 -6.577512506668661 2210 17 -6.153861174932064 2210 18 -6.101466562368287 2210 19 -11.023265315361053 2210 20 -8.353608468880395 2210 21 -6.535295081904152 2210 22 -10.411845621956076 2210 23 -8.58386796427586 2210 24 -10.722612633079848 2210 25 -8.661692499790323 2211 1 0.006405109205534387 2211 2 4.231341516337425 2211 3 0.39008232699796963 2211 4 1.1571407147822832 2211 5 0.3965862623874514 2211 6 -0.42697431098046934 2211 7 -19.194109206257856 2211 8 -12.721527016546526 2211 9 -9.050215548790025 2211 10 -7.114107820169764 2211 11 -9.282430150307889 2211 12 -5.004476788512211 2211 13 -6.187980592456148 2211 14 -4.933098168915194 2211 15 -5.4426427220771565 2211 16 -11.107403363001303 2211 17 -7.835782552899868 2211 18 -6.019013449447275 2211 19 -8.426442291262005 2211 20 -6.7504336912406195 2211 21 -4.786669911080942 2211 22 -6.053963817574452 2211 23 -7.927783482656377 2211 24 -20.47778129466853 2211 25 -20.249287711977484 2212 1 -1.871900709628921 2212 2 -13.10535882036291 2212 3 -7.735536316608927 2212 4 -18.426473202164967 2212 5 -18.574233041765186 2212 6 -19.4572773412835 2212 7 -9.626928589517767 2212 8 -9.368256177959974 2212 9 -5.772434062858757 2212 10 -5.988129485674545 2212 11 -6.089985636644579 2212 12 -3.6218299579946027 2212 13 -5.933345400596286 2212 14 -4.321922783143627 2212 15 -4.614000202906002 2212 16 -4.600461699671975 2212 17 -4.469252593491788 2212 18 -3.7450661942224848 2212 19 -4.2632958983065095 2212 20 -4.4521485886236505 2212 21 -2.503778589643364 2212 22 -3.699778301127555 2212 23 -3.804711104444497 2212 24 -2.921613893787473 2212 25 -3.294441896999512 2213 1 -17.552517449717026 2213 2 -13.10535882036291 2213 3 -8.220533154663741 2213 4 -5.778054710680783 2213 5 -18.574233041765186 2213 6 -10.215184883862577 2213 7 -19.194109206257856 2213 8 -9.94337649265391 2213 9 -8.122962482850827 2213 10 -6.972759216534058 2213 11 -6.15369347290941 2213 12 -4.96022240362522 2213 13 -17.88158328605884 2213 14 -11.557927156274594 2213 15 -6.745427258438333 2213 16 -5.851707720444349 2213 17 -4.502723659013725 2213 18 -4.589597636819782 2213 19 -5.550132559721131 2213 20 -5.450602451020085 2213 21 -3.7948785178296944 2213 22 -4.547983495292428 2213 23 -4.331156220578993 2213 24 -3.5653725355444443 2213 25 -4.276051702245123 2214 1 -17.552517449717026 2214 2 -13.10535882036291 2214 3 -12.156420771127548 2214 4 -18.426473202164967 2214 5 -18.574233041765186 2214 6 -19.4572773412835 2214 7 -15.823692012831813 2214 8 -14.574950987964598 2214 9 -14.124251313091275 2214 10 -14.252936761358589 2214 11 -14.124399499651869 2214 12 -12.575855699124158 2214 13 -17.88158328605884 2214 14 -16.27196371722027 2214 15 -22.183383462817897 2214 16 -17.636614564778878 2214 17 -21.847005897456548 2214 18 -17.214180458773576 2214 19 -14.88860429619687 2214 20 -13.822143910443463 2214 21 -11.587705977603733 2214 22 -12.557425176493037 2214 23 -12.484485493635674 2214 24 -11.491687742203299 2214 25 -11.207568369373185 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 120068.0 3 7.0 4 598.0 5 2031.0 6 2752.0 7 2612.0 8 2240.0 9 2051.0 10 1965.0 11 1916.0 12 1749.0 13 1827.0 14 2744.0 15 5439.0 16 7795.0 17 8643.0 18 9133.0 19 10560.0 20 12498.0 21 14301.0 22 14697.0 23 15782.0 24 16306.0 25 16147.0 26 13705.0 27 11452.0 28 12154.0 29 13962.0 30 13566.0 31 15641.0 32 19886.0 33 24213.0 34 28769.0 35 32105.0 36 30186.0 37 29549.0 38 19010.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.53657541949802 18.234695673450467 12.45852238825191 25.770206518799604 2 12.725939969148522 20.778823169223884 42.34394686951709 24.1512899921105 3 21.094967444517778 24.26796954264719 27.75342546715393 26.883637545681104 4 11.518807450411224 16.604032759693137 36.990721542608334 34.8864382472873 5 12.020365296014246 38.05332491982678 35.28810497290102 14.638204811257962 6 31.599852854878126 36.512354059190876 18.491455948429138 13.396337137501863 7 27.174331778726753 30.72690835471259 21.094973074432595 21.00378679212806 8 25.24762270093477 38.88554642547092 19.611758292786334 16.255072580807976 9 26.367602869077412 16.112232652035985 19.520145872677713 38.00001860620889 10 14.947219922333979 30.359153481863153 33.68513765066887 21.00848894513399 11 33.19492752909182 22.36360590802944 24.163012740296978 20.278453822581756 12 24.491563203970443 25.064997060830933 31.367837994579308 19.075601740619316 13 28.882416987849602 21.473723116932852 24.39498487044694 25.248875024770605 14 19.979715641242596 23.997302973528345 26.994628611144687 29.02835277408437 15 23.29007580925808 32.32586483109636 22.602311837435515 21.781747522210043 16 20.500801965342067 28.364661401992553 30.29340242180119 20.841134210864187 17 21.17835035548224 28.99570279347162 29.52600451752697 20.29994233351917 18 21.01282305318878 27.281116426529284 32.18010775516366 19.525952765118276 19 23.093694655423132 26.90281610181799 26.988632612914724 23.014856629844154 20 23.035848078994746 28.299555300423823 28.569058899396506 20.09553772118492 21 21.07793207476752 26.600744876061334 26.88046216527229 25.440860883898857 22 21.904907639353304 29.468128114739546 27.385904658271276 21.241059587635878 23 20.93248689219411 28.94471464241459 28.46263763661329 21.660160828778004 24 22.40163519771269 28.243002177123206 28.77796415427058 20.57739847089352 25 22.145046079305153 27.658561000887538 28.360216804386695 21.83617611542061 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 818.0 1 818.0 2 1478.5 3 2139.0 4 2139.0 5 2139.0 6 2925.0 7 3711.0 8 3711.0 9 3711.0 10 4274.0 11 4837.0 12 4837.0 13 4837.0 14 5781.5 15 6726.0 16 6726.0 17 6726.0 18 9167.0 19 11608.0 20 11608.0 21 11608.0 22 16075.5 23 20543.0 24 20543.0 25 20543.0 26 27187.0 27 33831.0 28 33831.0 29 33831.0 30 41442.0 31 49053.0 32 49053.0 33 49053.0 34 53719.0 35 58385.0 36 58385.0 37 58385.0 38 60467.5 39 62550.0 40 62550.0 41 62550.0 42 63894.5 43 65239.0 44 65239.0 45 65239.0 46 66983.5 47 68728.0 48 68728.0 49 68728.0 50 62466.0 51 56204.0 52 56204.0 53 56204.0 54 47167.0 55 38130.0 56 38130.0 57 38130.0 58 31742.5 59 25355.0 60 25355.0 61 25355.0 62 20360.5 63 15366.0 64 15366.0 65 15366.0 66 11815.5 67 8265.0 68 8265.0 69 8265.0 70 6096.5 71 3928.0 72 3928.0 73 3928.0 74 2772.0 75 1616.0 76 1616.0 77 1616.0 78 1137.0 79 658.0 80 658.0 81 658.0 82 447.0 83 236.0 84 236.0 85 236.0 86 164.0 87 92.0 88 92.0 89 92.0 90 60.5 91 29.0 92 29.0 93 29.0 94 19.5 95 10.0 96 10.0 97 10.0 98 6.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.408380865295442 2 36.867146539691745 3 5.661832624303282 4 13.948470335037607 5 13.996792173349018 6 15.12231929955637 7 9.301396315273976 8 7.945039484517497 9 0.11225534746189544 10 1.457089278313345 11 5.689710607944482 12 5.782451366857537 13 16.52941406053983 14 1.595921636846517 15 3.0150968574078307 16 3.0137958848379083 17 2.99075008502785 18 1.5154471907355884 19 1.4604346363502887 20 0.28193934122466124 21 4.388180478349028 22 1.6208259688993216 23 0.11021096199487417 24 9.255490568878134 25 0.11448558615319138 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 538059.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.2165229192083 #Duplication Level Percentage of deduplicated Percentage of total 1 96.84401623210967 86.40086393760193 2 2.1147147322577915 3.7733499075612937 3 0.4133519112239227 1.1063346078422296 4 0.19452373157158065 0.6941892382434339 5 0.10154438863126215 0.4529718637818997 6 0.0706707997231484 0.37830018139314286 7 0.042157954985487786 0.26328303100328065 8 0.03269097439248479 0.23332600529106973 9 0.02555461951908957 0.20519048682148527 >10 0.13281582639629252 2.243545614276626 >50 0.012450425625316943 0.7436402424810072 >100 0.013979406029529512 2.3753199448176545 >500 0.0010921410960514073 0.6276893545193587 >1k 4.368564384205629E-4 0.5019955843656839 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 1301 0.2417950447813344 No Hit CCCATGTACTCTGCGTTGATACCAC 1275 0.2369628609501932 No Hit GTCCTACAGTGGACATTTCTAAATT 891 0.16559522282872324 No Hit CTGTAGGACGTGGAATATGGCAAGA 643 0.11950362320860723 No Hit GAGTACATGGGAAGCAGTGGTATCA 638 0.11857435708723393 No Hit GTCCTACAGTGTGCATTTCTCATTT 542 0.10073244755686644 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.8585322427466133E-4 0.0 5 0.0 0.0 0.0 1.8585322427466133E-4 0.0 6 0.0 0.0 0.0 1.8585322427466133E-4 0.0 7 0.0 0.0 0.0 1.8585322427466133E-4 0.0 8 0.0 0.0 0.0 1.8585322427466133E-4 0.0 9 0.0 0.0 0.0 1.8585322427466133E-4 0.0 10 0.0 0.0 0.0 1.8585322427466133E-4 0.0 11 0.0 0.0 0.0 3.7170644854932265E-4 0.0 12 0.0 0.0 0.0 3.7170644854932265E-4 0.0 13 0.0 0.0 0.0 3.7170644854932265E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGT 25 0.003466171 20.904522 6 CTGTAGG 505 0.0 17.509201 1 TGTAGGA 550 0.0 17.25186 2 TATTAGG 50 0.0019821248 16.50178 2 GTGTAGC 50 0.0023598603 16.076632 1 GTGTAAG 50 0.0023598603 16.076632 1 GTCCTAA 195 0.0 15.801817 1 GAACAGT 95 1.2623786E-9 15.403333 6 GTCCTAT 70 8.154482E-5 15.311077 1 GTAGGAC 600 0.0 14.847072 3 GTCCTAC 580 0.0 14.552123 1 TAGGACC 230 0.0 14.510372 4 TAGCCCT 45 0.0050202706 14.354346 4 GTCTTAG 75 1.4674816E-4 14.2903385 1 TAGGACA 160 0.0 14.130059 4 TTTAGGA 215 0.0 14.071285 2 CCTAGAC 55 0.0010171597 13.99724 3 AATAGTG 45 0.0060867295 13.943534 5 GCCGGTT 40 0.0065234057 13.799048 11 CGTTCAG 40 0.0065234057 13.799048 10 >>END_MODULE