FastQCFastQC Report
Thu 26 May 2016
SRR1926569_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926569_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538059
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC38310.7120037021962276No Hit
GTACATGGGAAGCAGTGGTATCAAC35930.6677706348188581No Hit
CTGTAGGACGTGGAATATGGCAAGA19340.359440135747195No Hit
GTCCTACAGTGGACATTTCTAAATT19120.35535136481315244No Hit
GAGTACATGGGAAGCAGTGGTATCA16710.31056073776295906No Hit
CATGTACTCTGCGTTGATACCACTG13830.2570350091718566No Hit
GTATCAACGCAGAGTACTTTTTTTT13310.24737064150957425No Hit
GTCCTACAGTGTGCATTTCTCATTT13050.24253845767843304No Hit
CTTTAGGACGTGAAATATGGCGAGG13030.2421667512298837No Hit
GTCCTAAAGTGTGTATTTCTCATTT12370.22990043842775607No Hit
ACGCAGAGTACATGGGAAGCAGTGG12060.22413898847524158No Hit
CTGTAGGACCTGGAATATGGCGAGA11620.2159614466071565No Hit
GCGTTGATACCACTGCTTCCCATGT10860.20183660156228223No Hit
GTATCAACGCAGAGTACATGGGAAG9730.18083518721924546No Hit
GGTATCAACGCAGAGTACTTTTTTT9550.17748982918230155No Hit
GATATACACTGTTCTACAAATCCCG9260.1721000856783364No Hit
GCTTCCCATGTACTCTGCGTTGATA8380.1557450019421662No Hit
ACTCTGCGTTGATACCACTGCTTCC7970.1481250197469051No Hit
CTGAAGGACCTGGAATATGGCGAGA6980.12972555054371362No Hit
TATCAACGCAGAGTACTTTTTTTTT6690.12433580703974842No Hit
GTGTATATCAATGAGTTACAATGAA6580.12229142157272717No Hit
TATCAACGCAGAGTACATGGGAAGC6440.1196894764328819No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6300.11708753129303663No Hit
GGTATCAACGCAGAGTACATGGGAA6230.11578655872311401No Hit
GTGTATATCAATGAGTTACAATGAG5930.11021096199487417No Hit
GTTCTACAGTGTGGTTTTTATCATT5840.10853828297640222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4450.015.2093584
CCAACGT551.9989553E-413.78317419
AGGACCT6550.013.5348745
GTAGGAC9750.013.3970653
CTAGACC500.001459115313.3456764
TGTAGGA9800.013.2326612
GGACCTG6550.013.0982656
TGTAGAA2800.012.9407642
TCCAACG1251.05501385E-1012.88726718
CTGTAGG10150.012.8606851
TAGGACA2450.012.8398644
CTAAACT603.9677945E-412.7101694
GGACCTA603.9677945E-412.7101696
TAGGACT905.1635834E-712.7101684
GACTGTT604.0722906E-412.6710797
GGCGAGG4050.012.40060219
GATATAC3550.012.3463641
CTAGGAC701.05220155E-412.2585253
GTCCTAC9550.012.1721161
TACACAG1501.2732926E-1112.0746595
Produced by FastQC (version 0.11.5)