FastQCFastQC Report
Thu 26 May 2016
SRR1926568_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926568_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645678
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC28310.43845384231768775No Hit
CCCATGTACTCTGCGTTGATACCAC27300.42281137037346783No Hit
GAGTACATGGGAAGCAGTGGTATCA14080.21806535146001566No Hit
CATGTACTCTGCGTTGATACCACTG10170.15750885116110508No Hit
GCGTTGATACCACTGCTTCCCATGT9240.1431053868956353No Hit
GTATCAACGCAGAGTACATGGGAAG8300.12854704667032174No Hit
ACGCAGAGTACATGGGAAGCAGTGG7880.12204225635688377No Hit
GTCCTACAGTGGACATTTCTAAATT7470.11569234200328957No Hit
CNCATGTACTCTGCGTTGATACCAC6770.1048510248142263No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAAC350.00501195419.6138862
CTGTAGG4250.018.8918821
TGTAGGA4750.018.4989932
TAGGACC1750.017.8110314
ATCCCGT950.017.44801310
CAACGCG300.00990984917.4294055
AAGACCT300.00990984917.4294055
GCCTAGG654.3742795E-516.4698451
CCGTTTC951.8189894E-1216.19771413
GTCCTAA2250.016.0580981
GTAGGAC4900.015.0237293
CCCGTTT1150.014.95492312
GTATAGC550.004488449514.5982721
CCAACGA1051.8189894E-1214.54799319
TCCCGTT1153.6379788E-1214.412209511
CTATAGG658.158908E-414.4111141
TTAAGCA450.005077542314.3307144
GTGTAAC751.4832239E-414.2738661
GTCCTAC5550.014.2256451
TCTACAC857.6565993E-714.2191213
Produced by FastQC (version 0.11.5)