##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926568_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 645678 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.712429415281303 27.0 10.0 31.0 2.0 31.0 2 16.60754431775591 22.0 2.0 31.0 2.0 31.0 3 20.393637385817698 25.0 15.0 31.0 2.0 31.0 4 22.38128757677976 28.0 7.0 35.0 2.0 37.0 5 23.370517502532223 30.0 7.0 35.0 2.0 37.0 6 24.007005039663735 31.0 7.0 35.0 2.0 37.0 7 23.83147327305561 31.0 10.0 35.0 2.0 37.0 8 24.046227376494166 31.0 13.0 35.0 2.0 37.0 9 26.932982074656408 32.0 17.0 37.0 2.0 39.0 10 27.707936463686234 32.0 25.0 38.0 2.0 39.0 11 24.66622836770031 30.0 10.0 37.0 2.0 39.0 12 25.89272671517382 31.0 17.0 37.0 2.0 39.0 13 22.38668500398031 28.0 2.0 37.0 2.0 39.0 14 25.95348920049932 31.0 17.0 38.0 2.0 40.0 15 27.10653142897853 31.0 19.0 38.0 2.0 40.0 16 27.467963907706316 32.0 23.0 39.0 2.0 40.0 17 26.7993659378204 31.0 17.0 38.0 2.0 40.0 18 26.90240026762566 31.0 18.0 38.0 2.0 40.0 19 27.31306471646858 31.0 23.0 39.0 2.0 40.0 20 27.587040289432192 32.0 25.0 39.0 2.0 40.0 21 25.359411347451825 31.0 10.0 38.0 2.0 40.0 22 26.356964307286294 31.0 17.0 38.0 2.0 40.0 23 26.357576067327678 31.0 11.0 39.0 2.0 40.0 24 25.373378061510536 31.0 2.0 39.0 2.0 40.0 25 25.17121537360728 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.811996216955851 1101 2 10.77546963415229 1101 3 7.312700000984581 1101 4 8.71780395601008 1101 5 8.47797491391917 1101 6 7.964035389847499 1101 7 7.210031676867132 1101 8 6.052865403693961 1101 9 5.658800319918242 1101 10 5.253747034581359 1101 11 8.26503801467025 1101 12 5.470970433771683 1101 13 10.1928796752969 1101 14 7.3750082884121255 1101 15 6.460298965473026 1101 16 6.018521808972189 1101 17 2.279671767317634 1101 18 4.9072211533032615 1101 19 5.469150812292973 1101 20 3.4101458375806253 1101 21 0.13371662428597375 1101 22 4.053408312880432 1101 23 5.310217751743675 1101 24 6.758439830218794 1101 25 7.275392854254051 1102 1 5.651175199875983 1102 2 10.770975282010493 1102 3 6.852052176024973 1102 4 8.109809859833604 1102 5 8.33034109724353 1102 6 6.813436364510256 1102 7 7.007243981818235 1102 8 7.269613181449579 1102 9 6.09865179354717 1102 10 5.441584027886371 1102 11 8.060995424700106 1102 12 5.421959723118871 1102 13 10.215715438638682 1102 14 7.364221178426817 1102 15 5.4544599643361416 1102 16 5.800409436871739 1102 17 6.61858148801625 1102 18 6.649058518921464 1102 19 5.424396771697538 1102 20 5.751196359151518 1102 21 4.77843343356189 1102 22 5.419834305660217 1102 23 6.002424438754598 1102 24 7.540277594743998 1102 25 7.079594342177142 1103 1 5.323098547721628 1103 2 9.432727282757327 1103 3 6.564436013894461 1103 4 8.163406112093195 1103 5 7.730610126616103 1103 6 7.077413586124262 1103 7 7.051915587762341 1103 8 6.593959915331155 1103 9 5.69653011079815 1103 10 4.8746432398085275 1103 11 5.4484240300756 1103 12 4.313191232653715 1103 13 8.762397551155757 1103 14 6.954964700929388 1103 15 5.931189347944869 1103 16 5.570888343101849 1103 17 3.452788105969084 1103 18 2.1764969689049174 1103 19 4.415616399808986 1103 20 4.707957313399557 1103 21 6.97279230346879 1103 22 2.8737513563514625 1103 23 4.843981414423141 1103 24 6.262450876817471 1103 25 2.321909182923637 1104 1 5.893412554794825 1104 2 10.888122450230512 1104 3 7.346715100149542 1104 4 8.088466498068811 1104 5 8.284419183275872 1104 6 7.64770368612179 1104 7 7.770215217614858 1104 8 7.629793324232828 1104 9 6.276348417023058 1104 10 5.539244280178515 1104 11 8.558740345418169 1104 12 5.401782611267599 1104 13 9.89836038801882 1104 14 7.6078570448945015 1104 15 6.8148855467198395 1104 16 6.70020583731057 1104 17 7.125152350876466 1104 18 4.338984294032905 1104 19 5.306198660758838 1104 20 4.652144184293103 1104 21 3.810508600674961 1104 22 5.779404967556459 1104 23 6.171603155023124 1104 24 7.3758264734638885 1104 25 6.694825763717187 1105 1 3.304840524909526 1105 2 9.607158544095608 1105 3 6.322827906631705 1105 4 6.061043578990624 1105 5 2.037539250623027 1105 6 4.5565219540371835 1105 7 5.7403614872139315 1105 8 2.988701037586509 1105 9 3.7318992956929478 1105 10 3.297225970171148 1105 11 6.6529947913826035 1105 12 3.8583963122041425 1105 13 4.942546434530449 1105 14 3.7224313898381993 1105 15 4.12434571719232 1105 16 4.396407036931741 1105 17 5.10259105444376 1105 18 2.3267565764700286 1105 19 2.3637699540346446 1105 20 1.6139772896282594 1105 21 2.552453249733155 1105 22 4.260105798872885 1105 23 1.460617150603472 1105 24 4.422990526920689 1105 25 5.20962109150841 1106 1 6.426065700319217 1106 2 11.209663707931874 1106 3 7.707772812316346 1106 4 8.804234950360868 1106 5 8.787112078797882 1106 6 7.8164457623362935 1106 7 7.557201621272746 1106 8 7.407580987435008 1106 9 6.2541411641577795 1106 10 5.4853053445633435 1106 11 7.69740619441902 1106 12 5.244830492379833 1106 13 4.727257460626909 1106 14 5.739648463851847 1106 15 5.760805214230647 1106 16 5.564366337061138 1106 17 6.061239375091869 1106 18 5.881892684745829 1106 19 5.7050967183788615 1106 20 5.2561471456838085 1106 21 6.068686582798584 1106 22 6.239550113830731 1106 23 6.420210634123396 1106 24 7.34816352548205 1106 25 6.449519663315872 1107 1 4.043920252141696 1107 2 -14.229945528302583 1107 3 -5.861700644767184 1107 4 1.6189677074719206 1107 5 4.519958312505636 1107 6 4.833890349932922 1107 7 4.654748715561393 1107 8 5.310273117122549 1107 9 3.5063113790528355 1107 10 2.8302616275543606 1107 11 5.646381127498849 1107 12 3.1109479341530317 1107 13 4.800658568874976 1107 14 4.061548558916016 1107 15 3.6625857911200477 1107 16 3.4205849095976006 1107 17 4.10827424863151 1107 18 -0.9112723891790715 1107 19 1.8982833693682757 1107 20 2.274715929612963 1107 21 -1.4125601337350773 1107 22 2.130221629603877 1107 23 -1.0384786686559764 1107 24 2.4725834464286898 1107 25 3.145985089978872 1108 1 5.649596441695181 1108 2 11.135488693537486 1108 3 0.5020025330270101 1108 4 6.351685272535558 1108 5 7.760714219400139 1108 6 7.680180148050951 1108 7 7.7801680414911765 1108 8 7.566648536448476 1108 9 6.276859863424242 1108 10 5.173370906616526 1108 11 7.700098747059723 1108 12 2.8648346465166377 1108 13 3.3811865562316186 1108 14 5.253586967354611 1108 15 5.484248138376419 1108 16 5.487717931287499 1108 17 0.5869461510693519 1108 18 4.197772789026882 1108 19 5.120489780664947 1108 20 5.466076907172212 1108 21 5.205164485692581 1108 22 2.051390625522643 1108 23 5.214911513364779 1108 24 6.026118651641045 1108 25 6.5454641872624855 1109 1 3.303291093948328 1109 2 -14.229945528302583 1109 3 -6.052326350875493 1109 4 1.7288208997091203 1109 5 0.9617953866438675 1109 6 2.1432489525288894 1109 7 3.182045293603622 1109 8 3.6742625349127103 1109 9 2.8810697330986805 1109 10 1.9760824078511128 1109 11 0.3373222286512174 1109 12 0.8502103804888002 1109 13 2.2902222880886534 1109 14 2.860262186492527 1109 15 2.7663586062640313 1109 16 2.6008496531390612 1109 17 3.1345257863784894 1109 18 3.297235117536804 1109 19 1.7363052618640715 1109 20 0.6504094848839834 1109 21 -0.08757143146799962 1109 22 2.3886400097600387 1109 23 2.649291221630481 1109 24 3.936811999565691 1109 25 4.307920830984621 1110 1 2.9626776208363133 1110 2 9.347012167466993 1110 3 6.327829311749593 1110 4 7.428623232883616 1110 5 8.188759259501744 1110 6 3.8481959178136513 1110 7 6.4026379411036665 1110 8 7.248096388886342 1110 9 5.5158890973880474 1110 10 5.061087967230584 1110 11 2.8431728137097245 1110 12 5.568085109138135 1110 13 10.237540549326546 1110 14 7.647823295544612 1110 15 6.810639504942134 1110 16 6.465359633548864 1110 17 6.850035572651233 1110 18 7.0120036532139025 1110 19 5.595564717221372 1110 20 5.982392683203816 1110 21 3.703740052621587 1110 22 6.124154737703421 1110 23 6.558470374839924 1110 24 7.640232341599898 1110 25 8.029378270846252 1111 1 5.280428227631951 1111 2 9.840673477800294 1111 3 7.616547111166849 1111 4 8.49144198206455 1111 5 8.590318683098854 1111 6 7.902074191430028 1111 7 7.916058071259663 1111 8 8.054365943479116 1111 9 6.467809224012747 1111 10 5.683062719195753 1111 11 7.7000867527060635 1111 12 7.046059584876904 1111 13 8.885152564233351 1111 14 6.55135279409167 1111 15 6.013889559677342 1111 16 6.092348051658334 1111 17 7.028376597686606 1111 18 6.673893905011532 1111 19 5.814221456478556 1111 20 5.125892299076472 1111 21 8.203331598524219 1111 22 7.53884040853729 1111 23 7.53908665791548 1111 24 7.527166283468361 1111 25 8.10870704068924 1112 1 -2.95522243003834 1112 2 7.523967515175677 1112 3 5.76863156588805 1112 4 -0.1342977548673332 1112 5 3.9758355323464194 1112 6 3.3346726524436363 1112 7 5.772464575941061 1112 8 6.009928659069885 1112 9 4.5606775762359355 1112 10 3.1436434401691784 1112 11 -0.5946935816254637 1112 12 3.985337095206308 1112 13 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2204 8 5.7789967566000655 2204 9 3.775450715981851 2204 10 3.7465677674792097 2204 11 5.338889626770339 2204 12 5.539220153677071 2204 13 3.6246070236690784 2204 14 4.833996540818319 2204 15 5.0860188577594485 2204 16 4.8326110592359015 2204 17 -0.6616606967942218 2204 18 3.1242568023037656 2204 19 3.9810458243157463 2204 20 4.35249384940029 2204 21 5.59234452105391 2204 22 5.310941383700143 2204 23 3.7334325171521563 2204 24 5.602048759176316 2204 25 5.597211732958346 2205 1 6.443122386624367 2205 2 10.917595455303974 2205 3 7.665132714220988 2205 4 8.479734081673413 2205 5 8.346276650585114 2205 6 8.160445995280416 2205 7 7.998478672717809 2205 8 7.660328595713583 2205 9 6.657508177328829 2205 10 5.899297197989725 2205 11 8.335749120520735 2205 12 7.353804664700252 2205 13 10.618446672725767 2205 14 8.014967387002933 2205 15 7.119290042127002 2205 16 6.666861750973723 2205 17 7.2229802441675695 2205 18 6.83826742713228 2205 19 5.724324314492577 2205 20 5.998894333368835 2205 21 -1.2402846777203003 2205 22 4.307812447736719 2205 23 5.892158661701394 2205 24 7.76641955048472 2205 25 8.15706317048376 2206 1 4.233779662920629 2206 2 9.209654835003501 2206 3 6.350647124866054 2206 4 1.9769497276584325 2206 5 6.028453310650725 2206 6 6.35001285700076 2206 7 6.640277236368341 2206 8 6.570482305952165 2206 9 5.1618038251006055 2206 10 4.085508937938005 2206 11 3.648279209907926 2206 12 4.995263249295476 2206 13 8.43256250383807 2206 14 0.8180351396172973 2206 15 3.0123430540436402 2206 16 3.8753011726619064 2206 17 4.886780664054108 2206 18 -0.8005879601708692 2206 19 1.2326624869438483 2206 20 2.957114133550487 2206 21 3.765544598198762 2206 22 -1.5960603355750251 2206 23 -2.095068548534705 2206 24 2.196617135392625 2206 25 3.6157668824594573 2207 1 1.3002633649702915 2207 2 -14.229945528302583 2207 3 -6.520332489990148 2207 4 -4.503315177563927 2207 5 1.242415347132166 2207 6 1.7943602995947892 2207 7 2.9464937707299477 2207 8 3.1404105300477525 2207 9 0.7192696339976692 2207 10 0.45320551833587075 2207 11 -1.6356840069903988 2207 12 -2.690581912798713 2207 13 0.5511829243943751 2207 14 -5.25495561050484 2207 15 -1.6037804569096465 2207 16 -0.41288613326608115 2207 17 0.6569280850071628 2207 18 0.5606507561913467 2207 19 0.001641787022215624 2207 20 -0.2694570505060163 2207 21 -3.9429981771580707 2207 22 -4.631609737661083 2207 23 -1.6717317067400792 2207 24 0.026490371735039986 2207 25 0.4595083777197573 2208 1 4.5143427873529625 2208 2 6.339882057904315 2208 3 6.0612777427995965 2208 4 8.119945123693388 2208 5 2.9901683659296268 2208 6 5.799875051244236 2208 7 6.405792911773144 2208 8 6.544786230284277 2208 9 4.885160334856625 2208 10 4.30078736820516 2208 11 6.557855144011718 2208 12 5.884909808849486 2208 13 8.603554392090132 2208 14 2.708361332106886 2208 15 4.2160097833492856 2208 16 4.870451349617028 2208 17 5.644056181608686 2208 18 0.37747821791175085 2208 19 2.905410615604314 2208 20 3.9646989310699823 2208 21 6.667163032850645 2208 22 5.529317064280907 2208 23 1.4906920452828274 2208 24 3.0762320622616173 2208 25 4.399026614991012 2209 1 3.62852048111478 2209 2 5.122606456574545 2209 3 5.756797917494858 2209 4 0.26127880467898024 2209 5 5.615003774312392 2209 6 6.34900157493891 2209 7 6.674657392576222 2209 8 5.228643214835479 2209 9 4.853418597029886 2209 10 4.007121701038745 2209 11 0.24065613482066084 2209 12 3.7996660177015826 2209 13 3.161086495603591 2209 14 4.6437941591716125 2209 15 4.926141130719081 2209 16 4.594501281172988 2209 17 1.9609227483253235 2209 18 4.139209852094659 2209 19 4.5229339191699225 2209 20 4.644767049184836 2209 21 7.080534064726013 2209 22 5.601308785811447 2209 23 1.1843723790289964 2209 24 -1.867449901104525 2209 25 1.655704797638819 2210 1 -1.6040021777517204 2210 2 -14.229945528302583 2210 3 -7.555997156640016 2210 4 -1.8596711997500748 2210 5 0.038444227998592595 2210 6 -0.6297653379330583 2210 7 -0.6269519570686057 2210 8 -21.64659308006055 2210 9 -13.181575664043228 2210 10 -6.90622521473567 2210 11 -10.53732655227872 2210 12 -4.345883157080312 2210 13 -5.068972853758531 2210 14 -3.6293900688626195 2210 15 -5.4439800746628855 2210 16 -4.667708309337147 2210 17 -3.99510048631857 2210 18 -4.177990720101796 2210 19 -10.003037316461558 2210 20 -6.547798920203782 2210 21 -4.458866636761208 2210 22 -9.43111285286421 2210 23 -7.2175023370873035 2210 24 -10.267982707223032 2210 25 -7.7093757640786755 2211 1 0.44815095449446574 2211 2 5.468060169703115 2211 3 1.2821597875322155 2211 4 2.013906547539868 2211 5 1.6186911415788394 2211 6 0.6555108373397935 2211 7 -21.4614484675372 2211 8 -13.8279795947804 2211 9 -9.378999536774511 2211 10 -6.949978096640763 2211 11 -9.46998157865259 2211 12 -4.3353582266484345 2211 13 -6.790976832326152 2211 14 -4.495634639992534 2211 15 -4.8482033024155236 2211 16 -11.797883784810063 2211 17 -7.569673064879183 2211 18 -5.338658763455115 2211 19 -8.563619577276455 2211 20 -6.671571003763173 2211 21 -4.163618478103281 2211 22 -6.079216026723447 2211 23 -8.247387988774218 2211 24 -23.017733454979762 2211 25 -22.832007867767608 2212 1 -1.0517105705127214 2212 2 -14.229945528302583 2212 3 -8.03841375229922 2212 4 -20.042123841823855 2212 5 -20.961555772001407 2212 6 -21.601849086686798 2212 7 -10.316094281704633 2212 8 -9.798387008303422 2212 9 -5.601162350167009 2212 10 -5.803925857058932 2212 11 -6.36159890541251 2212 12 -3.236721222460986 2212 13 -6.639988417921607 2212 14 -4.093880681660838 2212 15 -4.157546826110472 2212 16 -4.112166104305508 2212 17 -4.624393839011184 2212 18 -3.463426042469557 2212 19 -4.033260354798248 2212 20 -4.248882422227609 2212 21 -2.0328631385162943 2212 22 -3.5732659703287446 2212 23 -3.742827924263217 2212 24 -2.591790492698255 2212 25 -3.009560765651692 2213 1 -19.385657212647036 2213 2 -14.229945528302583 2213 3 -8.935974412372818 2213 4 -5.984506654323855 2213 5 -20.961555772001407 2213 6 -11.273724086686798 2213 7 -21.4614484675372 2213 8 -11.228624330060551 2213 9 -8.829791173764065 2213 10 -7.724535907656907 2213 11 -6.799041407712419 2213 12 -5.647235934684996 2213 13 -19.93437664239263 2213 14 -13.062227299358632 2213 15 -7.7500462511334725 2213 16 -6.977797572796767 2213 17 -5.59813805330511 2213 18 -5.511308150622732 2213 19 -6.4531966042232725 2213 20 -6.583462437464501 2213 21 -4.406604477925217 2213 22 -5.365321944339783 2213 23 -5.259745724113724 2213 24 -4.315585017479762 2213 25 -5.333960992767608 2214 1 -19.385657212647036 2214 2 -14.229945528302583 2214 3 -12.567892088584273 2214 4 -20.042123841823855 2214 5 -20.961555772001407 2214 6 -21.601849086686798 2214 7 -16.927307498374205 2214 8 -15.382275899443812 2214 9 -14.224873084557016 2214 10 -14.297704960520344 2214 11 -15.008292509034005 2214 12 -12.68317085346253 2214 13 -19.93437664239263 2214 14 -17.39976945932559 2214 15 -24.796921251133472 2214 16 -18.919203822796767 2214 17 -24.49559899080511 2214 18 -18.492805087572073 2214 19 -15.543393121293757 2214 20 -14.174747370008554 2214 21 -11.628473455072795 2214 22 -12.761883756013791 2214 23 -12.676101210071874 2214 24 -11.464870018856415 2214 25 -11.185532096842497 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 104356.0 3 2.0 4 362.0 5 1674.0 6 2561.0 7 2401.0 8 2110.0 9 1977.0 10 1855.0 11 1737.0 12 1668.0 13 1886.0 14 2928.0 15 6772.0 16 9890.0 17 10625.0 18 11137.0 19 12792.0 20 14955.0 21 17360.0 22 17983.0 23 19339.0 24 20259.0 25 20629.0 26 16885.0 27 13070.0 28 14161.0 29 16224.0 30 16125.0 31 18766.0 32 24613.0 33 31258.0 34 39004.0 35 44451.0 36 44288.0 37 46187.0 38 33388.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.44610296537548 18.079456740478843 12.255530517884152 25.218909776261526 2 13.512568973070355 19.991238389635704 41.43380308034594 25.062389556948006 3 20.66490752979889 24.193412643012383 27.704920467540955 27.436759359647777 4 12.287575332853512 16.63410736092847 36.7312260878506 34.34709121836742 5 12.481808619472341 37.734686820074636 36.29756775838965 13.48593680206337 6 32.49629885827326 36.18023873965373 17.76401630149345 13.559446100579557 7 28.31307238256787 30.911442939508426 21.30509650723483 19.47038817068887 8 25.26643334671871 37.671482600974166 20.034208311141054 17.027875741166074 9 26.746419717484432 15.862546919259337 19.29840881500672 38.09262454824951 10 15.229550753362226 29.83191623676244 33.546855493932014 21.391677515943318 11 32.889989271271766 22.797887265824873 23.656350581827184 20.655772881076174 12 24.35086364506399 25.078213175454923 31.138084206218775 19.432838973262314 13 28.947270494548434 21.071893722323523 24.48144978230938 25.499386000818664 14 20.74362264603045 23.265243066174406 26.575697481217116 29.415436806578033 15 24.663988271763824 30.021151112951422 22.898038206296093 22.41682240898866 16 21.144234830353373 27.877145380313056 29.489460279267405 21.48915951006617 17 22.36105650438079 28.306690576423314 28.55329989803192 20.77895302116397 18 22.229415545138366 26.99039770149705 30.617722667473156 20.162464085891425 19 23.383771512059035 27.167185952670714 27.063591150044005 22.385451385226247 20 23.45304343639388 27.80444847568031 27.770434923065697 20.972073164860117 21 21.586642296681244 26.77936971145004 26.999578462184477 24.634409529684238 22 22.445330852791397 28.575370110520755 26.266101187191577 22.71319784949627 23 21.36128252038823 28.172811560048373 27.662408136686413 22.80349778287699 24 22.622084688216066 28.601966067983643 27.42499090099773 21.350958342802564 25 22.89807075400757 26.818426369101054 26.858893555794328 23.424609321097048 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 877.0 1 877.0 2 1646.0 3 2415.0 4 2415.0 5 2415.0 6 3390.5 7 4366.0 8 4366.0 9 4366.0 10 5029.0 11 5692.0 12 5692.0 13 5692.0 14 6520.0 15 7348.0 16 7348.0 17 7348.0 18 10019.5 19 12691.0 20 12691.0 21 12691.0 22 17661.0 23 22631.0 24 22631.0 25 22631.0 26 30147.5 27 37664.0 28 37664.0 29 37664.0 30 46087.0 31 54510.0 32 54510.0 33 54510.0 34 59974.5 35 65439.0 36 65439.0 37 65439.0 38 68854.5 39 72270.0 40 72270.0 41 72270.0 42 75569.0 43 78868.0 44 78868.0 45 78868.0 46 84107.0 47 89346.0 48 89346.0 49 89346.0 50 80656.5 51 71967.0 52 71967.0 53 71967.0 54 60200.5 55 48434.0 56 48434.0 57 48434.0 58 40330.0 59 32226.0 60 32226.0 61 32226.0 62 26062.0 63 19898.0 64 19898.0 65 19898.0 66 15365.5 67 10833.0 68 10833.0 69 10833.0 70 7903.0 71 4973.0 72 4973.0 73 4973.0 74 3500.5 75 2028.0 76 2028.0 77 2028.0 78 1418.0 79 808.0 80 808.0 81 808.0 82 543.0 83 278.0 84 278.0 85 278.0 86 185.0 87 92.0 88 92.0 89 92.0 90 55.5 91 19.0 92 19.0 93 19.0 94 11.5 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.881067652916778 2 37.071109748202666 3 6.148420729837474 4 14.14094951353461 5 14.01162808706507 6 15.367567115497199 7 9.608969176586472 8 7.821390848069782 9 0.1063997844126639 10 1.6198476640058979 11 6.1683997286573184 12 6.139592800126379 13 16.760366622372143 14 1.753815369270751 15 3.1252419936872564 16 3.1926130362192917 17 3.0962801891964724 18 1.677306645107933 19 1.6279012139177733 20 0.281409619036114 21 4.841577380675817 22 1.766824949897627 23 0.10810341997094527 24 9.362096896595515 25 0.11011680744891417 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 645678.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.76866786174855 #Duplication Level Percentage of deduplicated Percentage of total 1 95.9128358654942 83.2222899889147 2 2.8706776141118535 4.981697448740563 3 0.5356317449638014 1.3942815892491867 4 0.21362669786318214 0.7414441597317023 5 0.1204016790315084 0.5223546648940903 6 0.07620844200281192 0.3967502995441989 7 0.0436580755947634 0.2651707142535595 8 0.035007634672410214 0.24300526604126355 9 0.018666373900876387 0.14576907574695194 >10 0.1427605293114124 2.2829391424228462 >50 0.014030339105114626 0.8372037742695173 >100 0.013651037750402094 2.418482664063381 >500 0.002085575211633714 1.2348228607173601 >1k 7.583909860486233E-4 1.3137883514107167 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2831 0.43845384231768775 No Hit CCCATGTACTCTGCGTTGATACCAC 2730 0.42281137037346783 No Hit GAGTACATGGGAAGCAGTGGTATCA 1408 0.21806535146001566 No Hit CATGTACTCTGCGTTGATACCACTG 1017 0.15750885116110508 No Hit GCGTTGATACCACTGCTTCCCATGT 924 0.1431053868956353 No Hit GTATCAACGCAGAGTACATGGGAAG 830 0.12854704667032174 No Hit ACGCAGAGTACATGGGAAGCAGTGG 788 0.12204225635688377 No Hit GTCCTACAGTGGACATTTCTAAATT 747 0.11569234200328957 No Hit CNCATGTACTCTGCGTTGATACCAC 677 0.1048510248142263 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 1.5487595984376112E-4 0.0 0.0 1.5487595984376112E-4 0.0 11 1.5487595984376112E-4 0.0 0.0 1.5487595984376112E-4 0.0 12 1.5487595984376112E-4 0.0 0.0 1.5487595984376112E-4 0.0 13 1.5487595984376112E-4 0.0 0.0 1.5487595984376112E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCTAAC 35 0.005011954 19.613886 2 CTGTAGG 425 0.0 18.891882 1 TGTAGGA 475 0.0 18.498993 2 TAGGACC 175 0.0 17.811031 4 ATCCCGT 95 0.0 17.448013 10 CAACGCG 30 0.009909849 17.429405 5 AAGACCT 30 0.009909849 17.429405 5 GCCTAGG 65 4.3742795E-5 16.469845 1 CCGTTTC 95 1.8189894E-12 16.197714 13 GTCCTAA 225 0.0 16.058098 1 GTAGGAC 490 0.0 15.023729 3 CCCGTTT 115 0.0 14.954923 12 GTATAGC 55 0.0044884495 14.598272 1 CCAACGA 105 1.8189894E-12 14.547993 19 TCCCGTT 115 3.6379788E-12 14.4122095 11 CTATAGG 65 8.158908E-4 14.411114 1 TTAAGCA 45 0.0050775423 14.330714 4 GTGTAAC 75 1.4832239E-4 14.273866 1 GTCCTAC 555 0.0 14.225645 1 TCTACAC 85 7.6565993E-7 14.219121 3 >>END_MODULE