FastQCFastQC Report
Thu 26 May 2016
SRR1926568_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926568_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645678
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC75021.161879450747896No Hit
GTACATGGGAAGCAGTGGTATCAAC72541.1234702127066434No Hit
GAGTACATGGGAAGCAGTGGTATCA34180.5293660307459755No Hit
CATGTACTCTGCGTTGATACCACTG27020.4184748434978426No Hit
GCGTTGATACCACTGCTTCCCATGT22910.3548208240020567No Hit
GTATCAACGCAGAGTACATGGGAAG20380.3156372061615852No Hit
ACGCAGAGTACATGGGAAGCAGTGG19420.3007691140165841No Hit
CTGTAGGACGTGGAATATGGCAAGA17210.2665415268911129No Hit
ACTCTGCGTTGATACCACTGCTTCC15760.24408451271376755No Hit
GTCCTACAGTGGACATTTCTAAATT15740.24377476079408003No Hit
GCTTCCCATGTACTCTGCGTTGATA15350.23773459836017333No Hit
TATCAACGCAGAGTACATGGGAAGC13350.2067594063914211No Hit
GTATCAACGCAGAGTACTTTTTTTT12730.19715709688110794No Hit
GGTATCAACGCAGAGTACATGGGAA11980.18554139989282584No Hit
GTCCTACAGTGTGCATTTCTCATTT11820.18306338453532564No Hit
GATATACACTGTTCTACAAATCCCG10710.16587215299266816No Hit
GTCCTAAAGTGTGTATTTCTCATTT10530.16308438571548048No Hit
CTTTAGGACGTGAAATATGGCGAGG10310.15967711459891773No Hit
CTGTAGGACCTGGAATATGGCGAGA10100.15642471944219874No Hit
CAGTGGTATCAACGCAGAGTACATG10030.15534058772329243No Hit
CATGGGAAGCAGTGGTATCAACGCA8580.13288357354594704No Hit
GTGGTATCAACGCAGAGTACATGGG7860.12173250443719624No Hit
GTGTATATCAATGAGTTACAATGAA7830.12126787655766497No Hit
GCAGTGGTATCAACGCAGAGTACAT7820.1211130005978212No Hit
GGTATCAACGCAGAGTACTTTTTTT7580.11739597756157093No Hit
ACATGGGAAGCAGTGGTATCAACGC7050.10918755168985159No Hit
ATACCACTGCTTCCCATGTACTCTG6880.10655466037250766No Hit
CCACTGCTTCCCATGTACTCTGCGT6700.10376689309531996No Hit
GTGTATATCAATGAGTTACAATGAG6660.10314738925594491No Hit
GTTCTACAGTGTGGTTTTTATCATT6460.10004987005906968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT603.8617145E-918.93781519
TAGGACC2750.016.6565384
CTACACT1100.015.6155044
CTTAGAC508.362674E-515.2708783
CCTAGAC456.5205444E-414.8466843
TCCAACG1950.014.56867818
TAGTAGA400.00510900614.3186822
GTCCTAT706.9177568E-613.6325831
CCAACGA1400.013.52701119
ACTGTTC3700.013.3583448
GTGCTAC655.2179028E-513.2131191
CATTTAC655.2179028E-513.2131191
CGGTTTC1450.013.06967113
AGGACCT5400.012.9004625
GATATAC4000.012.88279151
TGTAGGA8450.012.6524062
CACTGTT3950.012.5148257
GGACCTG5500.012.3179366
TTGTAGA1950.012.2343691
GGGTAAG701.082103E-412.2225837
Produced by FastQC (version 0.11.5)