##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926568_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 645678 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.59735502835779 31.0 28.0 31.0 23.0 34.0 2 28.998726919610085 31.0 28.0 33.0 24.0 34.0 3 29.3779732312391 31.0 30.0 33.0 25.0 34.0 4 33.11821217386995 35.0 33.0 37.0 28.0 37.0 5 32.91602160829392 35.0 33.0 37.0 26.0 37.0 6 33.07753090549779 35.0 33.0 37.0 27.0 37.0 7 32.89548815353783 35.0 33.0 37.0 26.0 37.0 8 32.886869306372525 35.0 33.0 37.0 26.0 37.0 9 34.10960726554103 38.0 34.0 39.0 25.0 39.0 10 34.01445147581302 37.0 34.0 39.0 25.0 39.0 11 34.28457992993412 38.0 34.0 39.0 27.0 39.0 12 34.08577495284027 38.0 34.0 39.0 26.0 39.0 13 34.24228640281998 38.0 34.0 39.0 27.0 39.0 14 34.89526048587686 39.0 34.0 40.0 25.0 41.0 15 35.03991308361134 39.0 34.0 40.0 26.0 41.0 16 34.9802780952735 39.0 34.0 40.0 25.0 41.0 17 34.930384804809826 39.0 34.0 40.0 25.0 41.0 18 34.905850904010975 39.0 34.0 40.0 25.0 41.0 19 34.909947373148846 39.0 34.0 40.0 25.0 41.0 20 34.93367746771611 39.0 34.0 41.0 25.0 41.0 21 34.83915202314466 39.0 34.0 41.0 25.0 41.0 22 34.80688671443041 39.0 34.0 41.0 25.0 41.0 23 34.459360548137 39.0 34.0 40.0 19.0 41.0 24 34.56079500927707 39.0 34.0 41.0 23.0 41.0 25 34.35026282450386 39.0 34.0 40.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5807815654973361 1101 2 0.6725115003100619 1101 3 0.5336093999625149 1101 4 0.3686738825413727 1101 5 0.46388798638581363 1101 6 0.201770268084708 1101 7 0.4803718086764306 1101 8 0.5393341665681533 1101 9 0.8202437282271049 1101 10 0.9069000135795591 1101 11 0.7896837648625521 1101 12 0.6325003940849641 1101 13 0.6058052194134191 1101 14 1.0174965595318355 1101 15 0.7029228793221236 1101 16 0.8897641592199008 1101 17 0.5902493239957636 1101 18 0.8730712034110297 1101 19 0.6653770901117113 1101 20 0.6841201793243243 1101 21 0.6850348993013071 1101 22 0.7834919935611708 1101 23 1.121473768561792 1101 24 1.0135412922252982 1101 25 1.2159724078982421 1102 1 0.34696721681273246 1102 2 0.44383806552137983 1102 3 0.31826943147616404 1102 4 0.1909824238049751 1102 5 0.2648666382131424 1102 6 -0.12219373720746063 1102 7 0.05370919756420989 1102 8 0.26239132329206427 1102 9 0.6037087008687294 1102 10 0.3058319401008873 1102 11 0.16258700997095588 1102 12 0.2743700046852524 1102 13 0.12154043824714478 1102 14 0.3761139478877453 1102 15 0.044955709367798136 1102 16 -0.019231858358601528 1102 17 0.053679525244341164 1102 18 0.16364835534805167 1102 19 0.2507498354561335 1102 20 0.039082582340064675 1102 21 0.06720774570217003 1102 22 0.16770092774816447 1102 23 0.2892806443886897 1102 24 0.1978496207385021 1102 25 0.23272317745632165 1103 1 0.18559032957592692 1103 2 0.1133240242249478 1103 3 0.10965624818823727 1103 4 -0.09355390364705585 1103 5 0.14058864685263472 1103 6 -0.29458385525788344 1103 7 -0.07631770537305016 1103 8 0.016398333331864023 1103 9 0.3978469157697333 1103 10 0.3807383738916599 1103 11 -0.0038488176126136864 1103 12 0.23780492418860888 1103 13 -0.09934625540732611 1103 14 0.3671214077242908 1103 15 0.21273838116044885 1103 16 0.08482479905752882 1103 17 0.2314960590936721 1103 18 0.16456831698803143 1103 19 0.23058196791703267 1103 20 0.26297442744024835 1103 21 0.22654781749991315 1103 22 0.12064200437486505 1103 23 0.5055743192280815 1103 24 0.3874270664022106 1103 25 0.33555350224351344 1104 1 0.5616412104280109 1104 2 0.713469115442809 1104 3 0.44174861193851456 1104 4 0.25547373787870953 1104 5 0.49378601212801954 1104 6 -0.010042665015269847 1104 7 0.21608314209156276 1104 8 0.3123704032724248 1104 9 0.7553376620826882 1104 10 0.32126004277041176 1104 11 0.2910771116719033 1104 12 0.29005837216598707 1104 13 0.2907586884110458 1104 14 0.6079753025166639 1104 15 0.5508462597784529 1104 16 0.5220497646395899 1104 17 0.3427760769003214 1104 18 0.3542198507505887 1104 19 0.5541741296937417 1104 20 0.43664531769028514 1104 21 0.5186502387096112 1104 22 0.31965604359895394 1104 23 0.7474949642244937 1104 24 0.4615983564774737 1104 25 0.7074379568736546 1105 1 0.8752790090179268 1105 2 0.6325683472643391 1105 3 0.7404963758025573 1105 4 0.5599565327855842 1105 5 0.6567135087080445 1105 6 0.5346590081405722 1105 7 0.51479933697739 1105 8 0.402794479879816 1105 9 1.0189491531907606 1105 10 0.936423675307509 1105 11 0.7436499833966224 1105 12 0.7257628865298713 1105 13 0.6695759040271696 1105 14 1.0488571293082032 1105 15 1.0007568143401713 1105 16 0.919113849665365 1105 17 0.7354099819829258 1105 18 0.8378881660194537 1105 19 1.0007594185498903 1105 20 1.1155256712628372 1105 21 0.8909221528419877 1105 22 1.0202979461560417 1105 23 1.270371950439376 1105 24 0.9352803516368979 1105 25 0.9677031362906803 1106 1 0.6087406680359564 1106 2 0.7505071527906466 1106 3 0.6253850217924466 1106 4 0.5310149383858302 1106 5 0.691744813209624 1106 6 -0.5407273754058792 1106 7 -0.1228737620477105 1106 8 0.03956340523927082 1106 9 0.19287519580345958 1106 10 0.19132108173558038 1106 11 -0.1346466337883001 1106 12 -0.049853358055131025 1106 13 -0.17021577436026547 1106 14 0.1569987108485975 1106 15 0.0709166722442589 1106 16 -0.12407993283465402 1106 17 0.05879713709090595 1106 18 -0.05298249294231283 1106 19 -0.03940518124275627 1106 20 -0.07095833892820025 1106 21 0.018043344261585048 1106 22 -0.03979705235709474 1106 23 0.21632728024174241 1106 24 -0.13666293859078849 1106 25 -0.1019447583911699 1107 1 0.9831433898731952 1107 2 0.7874883651089633 1107 3 0.8880451298653966 1107 4 0.7447639064392675 1107 5 0.7332478277444352 1107 6 0.4677392770845543 1107 7 0.8001524056154636 1107 8 0.7466516272891539 1107 9 0.9813511670840711 1107 10 0.5376329672583111 1107 11 0.541234586035074 1107 12 0.8295245970645766 1107 13 0.6013847434892838 1107 14 0.8847805886160884 1107 15 0.6997004763120032 1107 16 0.749189211337665 1107 17 0.7838318480207818 1107 18 0.6857364548474436 1107 19 0.8142241435514848 1107 20 0.838261634553902 1107 21 0.8135115352905302 1107 22 0.7002243370807761 1107 23 0.9372279506943002 1107 24 0.7290323432244321 1107 25 0.9663982481382405 1108 1 1.3738219409734356 1108 2 1.1874284181529369 1108 3 1.2714838749757824 1108 4 0.7847807703051828 1108 5 -0.2520681956530453 1108 6 0.26932166478997743 1108 7 0.9170777322961001 1108 8 1.1260923948524066 1108 9 1.305104088741274 1108 10 1.310081285715917 1108 11 1.0911177197470678 1108 12 1.1409475198117178 1108 13 1.217824893417223 1108 14 1.5560638773424316 1108 15 1.143491122164761 1108 16 1.291676616421654 1108 17 1.2628950708660227 1108 18 1.3340196406345655 1108 19 1.3125937680049944 1108 20 1.5042208464028093 1108 21 1.3981911521144283 1108 22 1.3547497267359887 1108 23 1.575389789309824 1108 24 1.3369315657305805 1108 25 1.4313822622833001 1109 1 0.19668309512789506 1109 2 -1.0206196412177668 1109 3 0.1724859548509592 1109 4 0.41646277164475975 1109 5 -1.031658903193776 1109 6 -0.18586752415066599 1109 7 0.3022393479468164 1109 8 0.3394734871470533 1109 9 0.7409291339942641 1109 10 -1.4685399884796837 1109 11 -0.30623346648683025 1109 12 0.04641282482687359 1109 13 0.37629467545182393 1109 14 0.7872089735424055 1109 15 0.6317728571209216 1109 16 0.7468527774042926 1109 17 0.7430611543399763 1109 18 0.814331330697911 1109 19 0.7104262223343412 1109 20 0.8829190621856426 1109 21 0.7117503442584194 1109 22 0.772087333239547 1109 23 0.8798056539907222 1109 24 0.6863567262426855 1109 25 0.6956888760411246 1110 1 0.4687926590254641 1110 2 -0.7684091260486028 1110 3 0.17122406395598944 1110 4 0.44665108459370373 1110 5 0.6942460402417439 1110 6 0.2820204411164511 1110 7 0.6657521698172388 1110 8 0.8398529368567296 1110 9 1.0051496736952998 1110 10 0.6813161736071152 1110 11 0.5537407662305611 1110 12 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24 0.3363123572229867 1111 25 0.4377580108996355 1112 1 0.12784639048556556 1112 2 -0.2466879974140035 1112 3 0.30357329887948126 1112 4 0.6215933614300866 1112 5 0.4056176991467666 1112 6 0.29847565185491476 1112 7 0.6155075801408785 1112 8 0.7386984735843214 1112 9 0.3393977071943013 1112 10 0.8810469292913794 1112 11 0.4671990685825378 1112 12 0.800525821804321 1112 13 0.30099435769626126 1112 14 0.5817156774097185 1112 15 0.5451046404271267 1112 16 0.539294845140482 1112 17 0.5740446465510942 1112 18 0.5315769324959447 1112 19 0.7302774197302924 1112 20 0.49469151820362356 1112 21 0.7110559321062055 1112 22 0.5840240921328288 1112 23 0.7436869219129107 1112 24 0.6541471625753204 1112 25 0.7435599137842885 1113 1 -0.03638285852629153 1113 2 0.07611832669666896 1113 3 -0.9891539738477917 1113 4 -0.520738654380196 1113 5 -1.4053309174540267 1113 6 -0.44709747067472705 1113 7 -0.09073747914766273 1113 8 -0.17339638807575852 1113 9 0.3046546241903485 1113 10 0.45689673166291556 1113 11 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19 0.15331322773158718 2202 20 0.10064606182317704 2202 21 0.15908871237345323 2202 22 0.1690654711647568 2202 23 0.2188142885916946 2202 24 0.2389116048667077 2202 25 0.31455987341450253 2203 1 0.24220195871831862 2203 2 0.2933723098269425 2203 3 0.044079390725777046 2203 4 -0.23611822326491705 2203 5 0.07649663643537963 2203 6 0.25004404760080945 2203 7 0.179759240627412 2203 8 0.45250801679771513 2203 9 0.6563415970113411 2203 10 0.6743381562177433 2203 11 0.3855724030024845 2203 12 0.5088694100851541 2203 13 0.4649526316052288 2203 14 0.9071531086472433 2203 15 0.7795759333654786 2203 16 0.7868862228349514 2203 17 0.8978105892592154 2203 18 0.6565865083078464 2203 19 0.6361663403121796 2203 20 0.6900014077761725 2203 21 0.7502401544280346 2203 22 0.6127621223679398 2203 23 0.7737174830147424 2203 24 0.6712695292270467 2203 25 0.4050260317093901 2204 1 0.2294561119406282 2204 2 0.3746218739660989 2204 3 0.16448073989581502 2204 4 0.09983055862096535 2204 5 0.10467238233148635 2204 6 0.06536746910418856 2204 7 0.08480451062300176 2204 8 0.26460394190279146 2204 9 0.6733644080543968 2204 10 0.4806271391864314 2204 11 0.38702073669584536 2204 12 0.42751864118410055 2204 13 0.46962846169689243 2204 14 0.967865760263031 2204 15 0.647762258986269 2204 16 0.5702204349771449 2204 17 0.41675118801720146 2204 18 0.5257440594587806 2204 19 0.5593833930594201 2204 20 0.7543616831211679 2204 21 0.5198904622095739 2204 22 0.644963096314946 2204 23 0.7685381804941471 2204 24 0.7041432171795989 2204 25 0.5411311281770921 2205 1 0.684059267653538 2205 2 0.6526657683260773 2205 3 0.7692199291162751 2205 4 0.4001472538827642 2205 5 0.3751575740180684 2205 6 0.41835210706992854 2205 7 0.5563961522482685 2205 8 0.7230732260987693 2205 9 1.0375160250845354 2205 10 1.097925047609266 2205 11 0.959485439058497 2205 12 0.9784477351645791 2205 13 0.9284117965178353 2205 14 1.1794681126675002 2205 15 0.9989353207153968 2205 16 0.9955061384009554 2205 17 0.9433231766849346 2205 18 0.9022841471946066 2205 19 0.9926311062749988 2205 20 1.089108244266825 2205 21 0.8881478779365679 2205 22 0.9779989080035776 2205 23 1.3392408935609694 2205 24 1.2886525874585075 2205 25 1.3093617669560658 2206 1 0.427265890544799 2206 2 0.7582032082093448 2206 3 0.7207940073263472 2206 4 0.718528953579515 2206 5 0.9984037039645202 2206 6 0.6013749923753906 2206 7 0.8413457509737157 2206 8 0.7914884273812604 2206 9 1.1018018457244878 2206 10 0.9451578166270025 2206 11 0.9098559417808119 2206 12 0.9974369302827739 2206 13 0.7027674493520664 2206 14 1.0676249298679608 2206 15 1.0616105836408067 2206 16 0.9485046544047862 2206 17 1.2244660026305496 2206 18 0.9502311203883735 2206 19 0.8776635904051773 2206 20 1.0083470882387715 2206 21 0.9761453764848298 2206 22 1.0386218886024423 2206 23 1.4180912184022745 2206 24 1.109409177344503 2206 25 1.3757172411151757 2207 1 -0.8542364850441793 2207 2 0.32052594074327345 2207 3 0.3061725768578185 2207 4 0.41921539878685365 2207 5 0.3023356398154178 2207 6 0.5329977049078138 2207 7 0.5958952743804034 2207 8 0.7279623254129177 2207 9 1.0133845224509272 2207 10 0.6487772698972449 2207 11 0.5297559670286205 2207 12 0.6171574337961587 2207 13 0.3503900569585454 2207 14 -4.618785028915816 2207 15 -1.383719719955856 2207 16 -0.3557232054010342 2207 17 -0.2346604232966527 2207 18 0.2367896149633708 2207 19 0.3639330467904145 2207 20 0.5279829952088022 2207 21 0.7364789426226039 2207 22 0.6580407300232665 2207 23 0.8019791837242494 2207 24 0.5956712561242128 2207 25 0.8215680757682762 2208 1 0.3917519377119518 2208 2 0.7576073549098368 2208 3 0.5187457126725832 2208 4 0.34220805408375554 2208 5 0.25993554022622334 2208 6 0.16849814061053792 2208 7 0.19238164310939254 2208 8 0.310475085278469 2208 9 0.47409036086746426 2208 10 0.4892478288415987 2208 11 0.18252890366499486 2208 12 0.27420648147448645 2208 13 0.09739615679671232 2208 14 0.4054338183392545 2208 15 0.25506715916775846 2208 16 0.15429484514048397 2208 17 0.2356713332077618 2208 18 0.3629037690776542 2208 19 -0.12142422944511821 2208 20 0.08453409691426828 2208 21 0.3323677761841637 2208 22 0.22835692571603516 2208 23 0.2589193057210082 2208 24 0.32442452389466325 2208 25 0.33251043852192197 2209 1 -0.09691731461840547 2209 2 0.16972032016633776 2209 3 0.5406999529437542 2209 4 0.7909695429045627 2209 5 0.6140487010371203 2209 6 0.6885701698133317 2209 7 0.910781682220275 2209 8 0.832082216495472 2209 9 1.0081689737349393 2209 10 0.5966234888376931 2209 11 0.719940089376486 2209 12 0.8491836667948647 2209 13 0.7618639229136548 2209 14 0.9969141651223836 2209 15 1.093384413584026 2209 16 0.9469697033635427 2209 17 0.9866344386115813 2209 18 0.9991383691689109 2209 19 1.0534910303163088 2209 20 1.0162416127215792 2209 21 0.9970294670778515 2209 22 1.0565004626432213 2209 23 1.2618721771113997 2209 24 1.3295724933881772 2209 25 1.309268798472381 2210 1 -0.33378610333731373 2210 2 -0.5486265296433999 2210 3 0.14612564185056698 2210 4 0.3406145587078129 2210 5 0.6618212623701396 2210 6 0.7346436695409864 2210 7 1.0022907090151278 2210 8 0.9225122496474327 2210 9 1.1621503189243256 2210 10 1.0991508783138713 2210 11 0.8202356111288083 2210 12 0.5137834402533201 2210 13 0.5084285767781438 2210 14 0.9276361767811707 2210 15 0.6418335840310974 2210 16 0.8370216646818562 2210 17 0.7149636287349779 2210 18 0.7258090188455881 2210 19 0.892606952870004 2210 20 0.8582053246104877 2210 21 0.746539914940314 2210 22 0.8148680819026453 2210 23 1.22280695312363 2210 24 1.1962512849906872 2210 25 1.0436752200394324 2211 1 -1.0367929422697095 2211 2 -0.13931031873197597 2211 3 0.49738146602098254 2211 4 0.6669337966255924 2211 5 0.8358319937088865 2211 6 0.6646740910968347 2211 7 0.9847507363405583 2211 8 0.6956496276369251 2211 9 1.0625109653800209 2211 10 1.1026266807262033 2211 11 0.8594200521078648 2211 12 1.0320027613972016 2211 13 0.9004121701575514 2211 14 1.2726211648369485 2211 15 1.0434918570752174 2211 16 1.0147174472297493 2211 17 1.1274886368066745 2211 18 1.2291177062686032 2211 19 1.2022118513748694 2211 20 1.1344743333487557 2211 21 1.2136192088723803 2211 22 1.2750658362180474 2211 23 1.4833879830632526 2211 24 1.324934560898953 2211 25 1.4509945725232924 2212 1 0.8383139717823127 2212 2 0.2530448132735721 2212 3 0.18611239731554363 2212 4 0.26984343142728306 2212 5 0.4615930601323228 2212 6 0.33690771457240487 2212 7 0.40980780813175954 2212 8 0.45181394896530236 2212 9 0.7098356896749962 2212 10 0.2292234022324564 2212 11 0.16496315801895634 2212 12 0.3265071825498893 2212 13 0.3190216366051075 2212 14 0.38600990564059146 2212 15 0.43773933503934614 2212 16 -0.4046057988337566 2212 17 0.2956933806017332 2212 18 0.06981380302111972 2212 19 0.08688795530887461 2212 20 0.20382715277823849 2212 21 0.36998237504848674 2212 22 0.2395122726056087 2212 23 0.6262828180770654 2212 24 0.5228804132452964 2212 25 0.1678005441590713 2213 1 0.2475304937464351 2213 2 0.14629943465121542 2213 3 -0.2836923261205193 2213 4 -1.2482351032438288 2213 5 -1.811481485635845 2213 6 -0.7302828399929098 2213 7 -0.5428823655112964 2213 8 -0.8303317573939424 2213 9 -1.139770091718745 2213 10 -1.1834690353825366 2213 11 -0.9312978743522464 2213 12 -0.9284942189565513 2213 13 -0.8160063216515638 2213 14 -7.518347162264192 2213 15 -3.1398681856598287 2213 16 -2.402150467359519 2213 17 -1.529603451274994 2213 18 -5.984900649025796 2213 19 -3.682543572117531 2213 20 -6.056420913861594 2213 21 -2.7620826548503175 2213 22 -2.2557890872149997 2213 23 -4.9274424938479555 2213 24 -2.9106711118812 2213 25 -2.389937029150495 2214 1 0.34507489077286735 2214 2 0.3511323435168556 2214 3 -1.4884090799751455 2214 4 -1.8514194713605683 2214 5 -0.393512735635845 2214 6 -0.24962049830789113 2214 7 -0.2933125692557894 2214 8 -0.1490912218763185 2214 9 -4.789558431476454 2214 10 -1.3420980620895833 2214 11 -0.4659938063456366 2214 12 -0.603178449408091 2214 13 -0.8529608233035475 2214 14 -2.1169799747641918 2214 15 -1.5598158728844993 2214 16 -1.001101630630444 2214 17 -1.2208720360767558 2214 18 -0.4435408610302005 2214 19 -3.9596248978333897 2214 20 -1.6974460033439698 2214 21 -3.234171035907586 2214 22 -5.468301132919844 2214 23 -5.658079023308307 2214 24 -4.733767030108073 2214 25 -2.7423642386769274 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18624.0 3 5.0 4 326.0 5 2078.0 6 2687.0 7 2236.0 8 1630.0 9 1368.0 10 1214.0 11 1113.0 12 1095.0 13 1134.0 14 1083.0 15 1123.0 16 1158.0 17 1234.0 18 1380.0 19 1656.0 20 2117.0 21 2766.0 22 3609.0 23 4457.0 24 5433.0 25 6395.0 26 7362.0 27 8163.0 28 9621.0 29 11552.0 30 14399.0 31 18310.0 32 23515.0 33 30784.0 34 40581.0 35 54333.0 36 74138.0 37 110869.0 38 176130.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.44866830564331 18.945836265613423 9.4365681937258 29.16892723501747 2 12.651905284227876 21.0825323641141 39.67106037789635 26.594501973761673 3 20.025655644241734 24.585574587278767 28.016242377670913 27.37252739080859 4 12.523587463398764 17.31776844779773 35.79872031233055 34.359923776472954 5 13.020820423844343 38.72877680009915 34.9265708266204 13.323831949436112 6 33.47885436392073 35.84932354486743 17.155623548287796 13.516198542924041 7 28.34944644176617 31.29171866947327 20.695409471651484 19.663425417109078 8 25.726253167072382 38.54559963237121 19.38403547121069 16.34411172934572 9 26.62569943424231 15.754115871645666 19.243524217037315 38.3766604770747 10 15.714329971962762 30.129033257431416 32.67500038725468 21.481636383351148 11 34.24706968330277 23.107064974971504 22.61269019180255 20.03317514992318 12 24.391275189614237 25.36006182887712 30.611489453313155 19.637173528195486 13 30.25579313527796 21.410207564761382 24.490845282013634 23.843154017947025 14 21.19538177540326 23.37057858003078 26.12514588884084 29.30889375572512 15 25.441676411142 29.9653845260313 22.0978371136735 22.4951019491532 16 21.559501591407177 28.05228798661824 29.374046510907526 21.014163911067055 17 22.543487789654 29.057014656751974 27.55912341378568 20.840374139808347 18 22.566370996095415 27.159719414978063 29.897421362314937 20.376488226611592 19 24.017557201069913 27.17741972954848 26.988930723707956 21.816092345673656 20 23.887545747266007 27.674792114201285 27.429309535720424 21.008352602812288 21 22.065212172649794 27.062602508684684 27.316289723809273 23.555895594856253 22 22.86000142485883 28.588553427559866 25.338016782359006 23.213428365222295 23 21.481140754369825 28.2290863247625 27.067206874014605 23.222566046853075 24 23.25698452941641 28.463457735059478 27.108755616198195 21.170802119325916 25 23.026183329771186 26.955386431007405 26.476819715090183 23.541610524131222 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 332.0 1 332.0 2 336.5 3 341.0 4 341.0 5 341.0 6 419.5 7 498.0 8 498.0 9 498.0 10 728.0 11 958.0 12 958.0 13 958.0 14 1422.5 15 1887.0 16 1887.0 17 1887.0 18 3424.0 19 4961.0 20 4961.0 21 4961.0 22 8437.0 23 11913.0 24 11913.0 25 11913.0 26 18270.0 27 24627.0 28 24627.0 29 24627.0 30 34618.5 31 44610.0 32 44610.0 33 44610.0 34 50092.0 35 55574.0 36 55574.0 37 55574.0 38 60375.0 39 65176.0 40 65176.0 41 65176.0 42 70569.5 43 75963.0 44 75963.0 45 75963.0 46 91593.0 47 107223.0 48 107223.0 49 107223.0 50 99942.0 51 92661.0 52 92661.0 53 92661.0 54 77015.0 55 61369.0 56 61369.0 57 61369.0 58 51828.5 59 42288.0 60 42288.0 61 42288.0 62 34945.5 63 27603.0 64 27603.0 65 27603.0 66 21655.5 67 15708.0 68 15708.0 69 15708.0 70 11527.0 71 7346.0 72 7346.0 73 7346.0 74 5163.5 75 2981.0 76 2981.0 77 2981.0 78 2056.5 79 1132.0 80 1132.0 81 1132.0 82 762.0 83 392.0 84 392.0 85 392.0 86 252.5 87 113.0 88 113.0 89 113.0 90 65.5 91 18.0 92 18.0 93 18.0 94 10.5 95 3.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08673053751250624 2 0.03252395156718984 3 0.03190444772781479 4 0.032214199647502315 5 0.024470401655314257 6 0.3828533727337775 7 0.22952617248845397 8 0.24005773775782976 9 0.24563327231220516 10 0.016726603663126203 11 0.0021682634378126556 12 0.003871898996094028 13 0.0 14 0.07666360012266175 15 0.0020133874779688947 16 0.0035621470764065064 17 0.005730410514219162 18 0.004181650915781551 19 0.002632891317343939 20 0.0017036355582813723 21 1.5487595984376115E-4 22 0.0 23 0.0 24 1.5487595984376115E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 645678.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.613498626504 #Duplication Level Percentage of deduplicated Percentage of total 1 90.33898555577078 65.59829803573727 2 6.886876749032614 10.001604307135642 3 1.3144829667372329 2.8634762129921087 4 0.49273352730365455 1.4311642123238555 5 0.2546760498722488 0.9246459498800998 6 0.15053049316259426 0.6558327455105405 7 0.10668351241009857 0.542266415730292 8 0.07588525668556625 0.4408235185687415 9 0.05240080501758693 0.34245052048550273 >10 0.2692259020066302 3.539405156325108 >50 0.027143940278794656 1.3568715870263193 >100 0.020465588394561307 2.877046959137734 >500 0.0056011084025755255 2.5887478319974284 >1k 0.003877690432552287 4.529096379226633 >5k 4.3085449250580967E-4 2.308270167922778 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 7502 1.161879450747896 No Hit GTACATGGGAAGCAGTGGTATCAAC 7254 1.1234702127066434 No Hit GAGTACATGGGAAGCAGTGGTATCA 3418 0.5293660307459755 No Hit CATGTACTCTGCGTTGATACCACTG 2702 0.4184748434978426 No Hit GCGTTGATACCACTGCTTCCCATGT 2291 0.3548208240020567 No Hit GTATCAACGCAGAGTACATGGGAAG 2038 0.3156372061615852 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1942 0.3007691140165841 No Hit CTGTAGGACGTGGAATATGGCAAGA 1721 0.2665415268911129 No Hit ACTCTGCGTTGATACCACTGCTTCC 1576 0.24408451271376755 No Hit GTCCTACAGTGGACATTTCTAAATT 1574 0.24377476079408003 No Hit GCTTCCCATGTACTCTGCGTTGATA 1535 0.23773459836017333 No Hit TATCAACGCAGAGTACATGGGAAGC 1335 0.2067594063914211 No Hit GTATCAACGCAGAGTACTTTTTTTT 1273 0.19715709688110794 No Hit GGTATCAACGCAGAGTACATGGGAA 1198 0.18554139989282584 No Hit GTCCTACAGTGTGCATTTCTCATTT 1182 0.18306338453532564 No Hit GATATACACTGTTCTACAAATCCCG 1071 0.16587215299266816 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1053 0.16308438571548048 No Hit CTTTAGGACGTGAAATATGGCGAGG 1031 0.15967711459891773 No Hit CTGTAGGACCTGGAATATGGCGAGA 1010 0.15642471944219874 No Hit CAGTGGTATCAACGCAGAGTACATG 1003 0.15534058772329243 No Hit CATGGGAAGCAGTGGTATCAACGCA 858 0.13288357354594704 No Hit GTGGTATCAACGCAGAGTACATGGG 786 0.12173250443719624 No Hit GTGTATATCAATGAGTTACAATGAA 783 0.12126787655766497 No Hit GCAGTGGTATCAACGCAGAGTACAT 782 0.1211130005978212 No Hit GGTATCAACGCAGAGTACTTTTTTT 758 0.11739597756157093 No Hit ACATGGGAAGCAGTGGTATCAACGC 705 0.10918755168985159 No Hit ATACCACTGCTTCCCATGTACTCTG 688 0.10655466037250766 No Hit CCACTGCTTCCCATGTACTCTGCGT 670 0.10376689309531996 No Hit GTGTATATCAATGAGTTACAATGAG 666 0.10314738925594491 No Hit GTTCTACAGTGTGGTTTTTATCATT 646 0.10004987005906968 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 1.5487595984376112E-4 0.0 0.0 0.0 0.0 9 1.5487595984376112E-4 0.0 0.0 0.0 0.0 10 3.0975191968752225E-4 0.0 0.0 0.0 0.0 11 3.0975191968752225E-4 0.0 0.0 1.5487595984376112E-4 0.0 12 3.0975191968752225E-4 0.0 0.0 1.5487595984376112E-4 0.0 13 3.0975191968752225E-4 0.0 0.0 1.5487595984376112E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGT 60 3.8617145E-9 18.937815 19 TAGGACC 275 0.0 16.656538 4 CTACACT 110 0.0 15.615504 4 CTTAGAC 50 8.362674E-5 15.270878 3 CCTAGAC 45 6.5205444E-4 14.846684 3 TCCAACG 195 0.0 14.568678 18 TAGTAGA 40 0.005109006 14.318682 2 GTCCTAT 70 6.9177568E-6 13.632583 1 CCAACGA 140 0.0 13.527011 19 ACTGTTC 370 0.0 13.358344 8 GTGCTAC 65 5.2179028E-5 13.213119 1 CATTTAC 65 5.2179028E-5 13.213119 1 CGGTTTC 145 0.0 13.069671 13 AGGACCT 540 0.0 12.900462 5 GATATAC 400 0.0 12.8827915 1 TGTAGGA 845 0.0 12.652406 2 CACTGTT 395 0.0 12.514825 7 GGACCTG 550 0.0 12.317936 6 TTGTAGA 195 0.0 12.234369 1 GGGTAAG 70 1.082103E-4 12.222583 7 >>END_MODULE