FastQCFastQC Report
Thu 26 May 2016
SRR1926567_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926567_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730609
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC18910.25882517187716003No Hit
GTACATGGGAAGCAGTGGTATCAAC18400.2518446939471044No Hit
GAGTACATGGGAAGCAGTGGTATCA8810.12058433443880379No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTTAG350.00590377119.0668661
AGACCCG453.296034E-416.239955
TGTAGAA2600.015.8038252
CCAGTAT505.0655263E-415.3478373
GTCCTAC4600.015.0876951
TTAGAAG450.004453615814.6169873
TCCTACA4950.014.11172
TGTAGGA3700.014.0668292
GTATTAA1059.249816E-713.982371
TAGACTG755.571408E-613.9199575
GTAGGAG652.3941259E-413.4926053
GGCGAGG1850.013.35482419
GTGTTTA600.00813132113.34680751
CCTTAGT600.00813132113.34680751
CCTACAG4950.013.2881713
TCCAACG1550.013.19953818
TAGATCA756.546937E-512.8962154
CCCTGAA853.5185285E-412.89096452
GTCCTAA1051.4201709E-512.7112461
CTACACT1109.96115E-812.700824
Produced by FastQC (version 0.11.5)