##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926567_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 730609 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.46360091375825 27.0 2.0 30.0 2.0 31.0 2 15.802819291851044 16.0 2.0 30.0 2.0 31.0 3 19.3680340647323 20.0 12.0 30.0 2.0 31.0 4 21.33461126265896 27.0 2.0 35.0 2.0 37.0 5 21.96308011535582 28.0 2.0 35.0 2.0 37.0 6 22.171608890665183 29.0 2.0 35.0 2.0 36.0 7 22.278508750918753 30.0 2.0 35.0 2.0 37.0 8 22.467384059052105 30.0 2.0 35.0 2.0 37.0 9 25.136024877875855 31.0 17.0 37.0 2.0 39.0 10 25.81692670087557 31.0 17.0 37.0 2.0 39.0 11 23.00875981544164 29.0 7.0 37.0 2.0 39.0 12 24.076499194507594 30.0 2.0 37.0 2.0 39.0 13 20.838063861792012 27.0 2.0 35.0 2.0 39.0 14 24.06880971901523 30.0 8.0 37.0 2.0 40.0 15 25.119195082458607 31.0 8.0 38.0 2.0 40.0 16 25.457815329403278 31.0 2.0 38.0 2.0 40.0 17 24.77515059354593 30.0 2.0 38.0 2.0 40.0 18 24.84137616700588 30.0 2.0 38.0 2.0 40.0 19 25.21376960864156 31.0 2.0 38.0 2.0 40.0 20 25.468844484532767 31.0 2.0 38.0 2.0 40.0 21 23.36030215888389 30.0 2.0 37.0 2.0 40.0 22 24.257403070589056 30.0 2.0 37.0 2.0 40.0 23 24.22741849607656 30.0 2.0 38.0 2.0 40.0 24 23.325524322859422 30.0 2.0 38.0 2.0 40.0 25 23.118249296135144 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.420647130443303 1101 2 9.773192906117458 1101 3 6.898281388411924 1101 4 8.170044414734598 1101 5 7.745011113706429 1101 6 7.072765295050733 1101 7 6.831850740264432 1101 8 5.1923601271341475 1101 9 5.242929238370834 1101 10 4.9867886797315 1101 11 7.512074339303993 1101 12 5.327995842096978 1101 13 9.728411788617858 1101 14 7.319041469834666 1101 15 6.474207828996214 1101 16 5.942753993786667 1101 17 2.1857943338557675 1101 18 4.473591125034417 1101 19 4.911610257344595 1101 20 3.328648940501882 1101 21 0.282189880053739 1101 22 4.154368322817707 1101 23 5.295485398698933 1101 24 6.583371532904231 1101 25 7.048064785160758 1102 1 5.386653512533098 1102 2 10.129941894930045 1102 3 6.590251777791835 1102 4 7.5917454602642245 1102 5 8.005056049620364 1102 6 6.410281350520847 1102 7 6.6121608161794825 1102 8 6.8251280319745256 1102 9 5.721957204962521 1102 10 5.04229242374425 1102 11 7.614930887154792 1102 12 5.344154144828444 1102 13 9.483335789492259 1102 14 7.199437433622062 1102 15 5.123797597991469 1102 16 5.580775416602059 1102 17 6.560267397530499 1102 18 6.538298065007332 1102 19 5.278129638382776 1102 20 5.72398150377628 1102 21 4.803177435366692 1102 22 5.811262441438689 1102 23 6.247762009387575 1102 24 7.574230424562632 1102 25 7.40209679391511 1103 1 4.287552468562993 1103 2 8.413811211657848 1103 3 5.64812354860398 1103 4 6.869120639891388 1103 5 6.495452968235316 1103 6 5.8912569474735825 1103 7 5.705801194397186 1103 8 5.4455474572988365 1103 9 4.58314527050101 1103 10 3.9115212244581627 1103 11 4.784038952168341 1103 12 3.7085161157075213 1103 13 7.609825464621359 1103 14 5.789842882476712 1103 15 4.68911036251092 1103 16 4.800488144375862 1103 17 2.5036207003736948 1103 18 1.0823816876032808 1103 19 3.05288997145583 1103 20 3.4334061062999197 1103 21 5.511006226894196 1103 22 1.6479090073610365 1103 23 3.492159938507843 1103 24 4.960868132159138 1103 25 1.458666062742335 1104 1 5.356140939013429 1104 2 9.842195852505249 1104 3 6.708115145884555 1104 4 7.064400475911047 1104 5 7.131099595689292 1104 6 6.595175033690577 1104 7 6.644034204353137 1104 8 6.734156043350325 1104 9 5.5620168871333 1104 10 4.421936777127936 1104 11 7.381630172599682 1104 12 4.416093840862207 1104 13 8.594310989027441 1104 14 6.369820857166701 1104 15 5.5515712134772315 1104 16 5.2687615128478384 1104 17 5.482099553529807 1104 18 3.3023266879852216 1104 19 3.8484931848594393 1104 20 3.1172964380800217 1104 21 2.2060117013932157 1104 22 4.285180160570622 1104 23 4.712066108140927 1104 24 5.993969117568959 1104 25 5.173162663460385 1105 1 3.6567388176041504 1105 2 9.41622830454681 1105 3 6.493706912608399 1105 4 6.110907346832359 1105 5 2.4555953666717265 1105 6 4.957463336365997 1105 7 5.99392133197286 1105 8 2.6653753607928046 1105 9 3.931067674775502 1105 10 3.8619616189094472 1105 11 7.252576131867048 1105 12 4.332755578603969 1105 13 5.570341627224639 1105 14 4.027282756577204 1105 15 4.524873691740673 1105 16 4.725367258002706 1105 17 5.3704051863051845 1105 18 2.9596283973363775 1105 19 2.680808695200323 1105 20 1.8641493195295205 1105 21 2.421578232021556 1105 22 4.152843455852654 1105 23 1.9961323726073203 1105 24 5.007535983364193 1105 25 5.5198459898932235 1106 1 6.524510338995544 1106 2 10.97079133970308 1106 3 7.99079868339685 1106 4 9.099539377219578 1106 5 8.803228422181885 1106 6 7.739788136912729 1106 7 7.468138499563128 1106 8 7.397778655052608 1106 9 6.3484598511560755 1106 10 5.941915017910077 1106 11 7.9807687460295895 1106 12 5.32802862282292 1106 13 4.761275727171647 1106 14 5.933895047157463 1106 15 5.9359306578719995 1106 16 5.907826760694274 1106 17 6.19717302814902 1106 18 6.331492982062542 1106 19 6.22404461709289 1106 20 5.405229759490496 1106 21 5.970155193002505 1106 22 6.023957769441228 1106 23 6.517593098513217 1106 24 7.151578137211871 1106 25 6.337023732344704 1107 1 3.534345468823993 1107 2 -13.29145194623689 1107 3 -5.388636006840969 1107 4 1.5803771573816334 1107 5 4.353646341184472 1107 6 4.618292012936802 1107 7 4.6995571504272675 1107 8 5.129325204052989 1107 9 3.526849329402733 1107 10 2.792484993585745 1107 11 5.674785481737565 1107 12 2.6182946272614593 1107 13 4.386990121159489 1107 14 3.650766469266312 1107 15 3.327040790009722 1107 16 3.2310916685247193 1107 17 3.671311551304502 1107 18 -1.2680532164022082 1107 19 1.5306792144861348 1107 20 1.7636654705449217 1107 21 -1.3715933639986169 1107 22 1.5825149837250798 1107 23 -1.1929094701076863 1107 24 1.9683510303861382 1107 25 2.8535995676497983 1108 1 5.303779516368987 1108 2 10.384538729753785 1108 3 0.48535415425563855 1108 4 6.176510326228442 1108 5 7.455662349497331 1108 6 7.539463711469821 1108 7 7.472480129497175 1108 8 7.276218540601501 1108 9 5.711129566906358 1108 10 5.048796814365332 1108 11 7.384137099634913 1108 12 2.919377768674437 1108 13 3.9630418388903657 1108 14 5.354051773001391 1108 15 5.365869860323048 1108 16 5.347905639161777 1108 17 0.7507805580598657 1108 18 4.257795747227867 1108 19 5.257879134339728 1108 20 5.509830266934607 1108 21 5.688129688228184 1108 22 2.373578659346478 1108 23 5.313081706798034 1108 24 5.965350199799101 1108 25 6.475711006103065 1109 1 4.13171392513199 1109 2 -13.29145194623689 1109 3 -5.104642544595649 1109 4 2.782911146276703 1109 5 1.9685649176395401 1109 6 3.1511305895867388 1109 7 4.350635551113196 1109 8 4.4062377226109355 1109 9 3.8034342294790946 1109 10 3.0475786084355114 1109 11 1.6372335834381389 1109 12 1.9071426890144991 1109 13 2.8768010645911026 1109 14 4.30467529876708 1109 15 4.233883875371994 1109 16 3.9129966542934085 1109 17 4.61703101488709 1109 18 4.729338014870866 1109 19 3.0713976854699787 1109 20 2.018524028268498 1109 21 1.2195010157002422 1109 22 3.6782197714611904 1109 23 4.121446552530873 1109 24 5.19446380665438 1109 25 5.458482544520422 1110 1 3.645628062821114 1110 2 9.341966638588346 1110 3 6.488146190295758 1110 4 7.9199132716774265 1110 5 8.43382683917077 1110 6 4.242083058590445 1110 7 6.726058605582043 1110 8 7.685914556435623 1110 9 5.862960685489394 1110 10 5.700986625122919 1110 11 3.9177816307218976 1110 12 6.290267464372317 1110 13 10.589815179218693 1110 14 8.158350912338893 1110 15 7.2722740557298025 1110 16 6.9072388890321115 1110 17 7.251212777162163 1110 18 7.48157197100409 1110 19 6.556416345920418 1110 20 6.660467069745323 1110 21 4.392024826905931 1110 22 6.696369821012318 1110 23 6.958999869340236 1110 24 8.030878590135675 1110 25 8.342722653165865 1111 1 5.612942346192973 1111 2 9.862567587947932 1111 3 7.899604733795474 1111 4 8.379994628886301 1111 5 8.752688555614444 1111 6 7.868937726894249 1111 7 7.708816795585907 1111 8 7.926664513361523 1111 9 6.493575770840167 1111 10 5.768261004903902 1111 11 7.675261952743234 1111 12 6.977318909260205 1111 13 8.557588706164506 1111 14 6.7340886323770945 1111 15 6.236226317456367 1111 16 5.774228627468236 1111 17 7.168087768755509 1111 18 6.836192557029634 1111 19 5.8171334704535695 1111 20 5.3697517962776224 1111 21 8.330112131863519 1111 22 7.5468562541116775 1111 23 7.311360746399387 1111 24 6.868192995203877 1111 25 7.498802698616245 1112 1 -1.6544976582506017 1112 2 6.9497931899498795 1112 3 5.2688926218407985 1112 4 -0.39698941225184115 1112 5 3.6802699943384383 1112 6 2.733431524892886 1112 7 5.237016241893208 1112 8 5.441561887267802 1112 9 4.056533320092214 1112 10 2.928966363795581 1112 11 -0.5739629067141223 1112 12 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2204 6 3.6831848242630976 2204 7 4.7520802544581215 2204 8 4.846448997429896 2204 9 3.224190389245532 2204 10 3.0743577368334734 2204 11 4.376951845026976 2204 12 4.538166768906734 2204 13 2.4784176429259652 2204 14 3.602347577998227 2204 15 3.9274091228932413 2204 16 4.037017214871291 2204 17 -1.5800054943756692 2204 18 2.328518838569515 2204 19 3.228473259573029 2204 20 3.5493291414850283 2204 21 4.780616811643142 2204 22 4.653435114460667 2204 23 3.077618430097644 2204 24 4.670530471989682 2204 25 4.84404942598788 2205 1 5.8542261726438625 2205 2 10.053640078302987 2205 3 6.827993025268743 2205 4 7.38398800533189 2205 5 7.729606367306378 2205 6 6.932781623959514 2205 7 7.285635277008151 2205 8 6.7540078780534145 2205 9 5.561457408798617 2205 10 5.0889410600310505 2205 11 7.443452528582242 2205 12 6.805881432018591 2205 13 9.442716475773594 2205 14 7.086363088134792 2205 15 6.153159939024171 2205 16 5.777988082281031 2205 17 6.530277390470811 2205 18 6.020682400451943 2205 19 4.862509081761086 2205 20 5.147874822923949 2205 21 -0.9554122674303365 2205 22 3.500752950631199 2205 23 4.734879319546568 2205 24 6.528854520972132 2205 25 6.938456678971438 2206 1 3.4060596117525286 2206 2 7.49935265146426 2206 3 5.119359995158032 2206 4 1.0481765909981569 2206 5 4.778583872418853 2206 6 4.9132278783374375 2206 7 5.128525999336148 2206 8 5.106719840068315 2206 9 3.7834710185581564 2206 10 2.8494398494911266 2206 11 2.457260910689758 2206 12 3.541916149833508 2206 13 6.6727972176112615 2206 14 -0.2528983649832277 2206 15 1.688893197139489 2206 16 2.553580424146908 2206 17 3.526500623435105 2206 18 -1.4785812857008906 2206 19 0.20752412536403142 2206 20 1.7798407162554 2206 21 2.515796274515459 2206 22 -2.710305272813315 2206 23 -3.495508170757361 2206 24 0.43501769705280324 2206 25 1.9846840254209148 2207 1 1.0238912364807078 2207 2 -13.29145194623689 2207 3 -6.165138296236812 2207 4 -4.402031252313133 2207 5 0.7780690719040528 2207 6 1.483869455875567 2207 7 2.4093792663963107 2207 8 2.70566657004953 2207 9 0.15253860831539967 2207 10 -0.12114255557403553 2207 11 -1.7674968519990415 2207 12 -2.486572437275772 2207 13 -0.0038459279973999116 2207 14 -5.584196165246546 2207 15 -1.6670617726085837 2207 16 -0.6261293348731947 2207 17 0.6154960227753321 2207 18 0.3696028590349769 2207 19 -0.07818720172660676 2207 20 -0.1814220243176834 2207 21 -3.665330665232169 2207 22 -4.181193895426077 2207 23 -1.5926101988938726 2207 24 0.04322787767677738 2207 25 0.5856071159991778 2208 1 5.406475193435373 2208 2 6.5575183054793555 2208 3 6.852125191507227 2208 4 8.724463009192569 2208 5 4.012146038704131 2208 6 6.969682107363273 2208 7 7.295403963862654 2208 8 7.581638521078688 2208 9 6.123695643087995 2208 10 5.556642388572463 2208 11 7.2360837799568785 2208 12 7.007008165229319 2208 13 9.343393498418227 2208 14 3.9504718797189824 2208 15 5.627548430926105 2208 16 5.87412541480947 2208 17 6.840345455405085 2208 18 1.4907252890818938 2208 19 4.148330914951693 2208 20 5.288232134581325 2208 21 8.0213154447549 2208 22 7.058329707941919 2208 23 2.992247951181671 2208 24 4.01993050380732 2208 25 5.439819615520602 2209 1 2.9585206511714723 2209 2 4.68477504762814 2209 3 5.155447012998806 2209 4 0.8448775758840377 2209 5 5.063195324361594 2209 6 5.533241746658899 2209 7 5.963549387437599 2209 8 4.635909718544212 2209 9 4.213297899596164 2209 10 3.4585729618715426 2209 11 -0.6109953241784929 2209 12 2.827353824656626 2209 13 1.951918929761323 2209 14 3.620105419423137 2209 15 4.108681411416811 2209 16 3.6897978982319515 2209 17 1.5992957953787865 2209 18 3.3527376151758688 2209 19 3.746711535748819 2209 20 3.8411263566662797 2209 21 5.988606137477639 2209 22 4.566703870876598 2209 23 0.5792044729208001 2209 24 -2.9259000802548627 2209 25 -0.07611387317579954 2210 1 -2.3500257426994295 2210 2 -13.29145194623689 2210 3 -7.59441110972015 2210 4 -2.4232729314495494 2210 5 -0.9671896523020393 2210 6 -1.5306299375711951 2210 7 -1.4301698521001747 2210 8 -19.956881692498733 2210 9 -12.59196207829055 2210 10 -6.9577463500725685 2210 11 -9.826082505321535 2210 12 -4.755733669594896 2210 13 -4.981341263387472 2210 14 -4.06131648447213 2210 15 -5.918287502781681 2210 16 -5.314037680676279 2210 17 -4.829823048859531 2210 18 -5.008858651005479 2210 19 -10.281216273176732 2210 20 -7.271403119561938 2210 21 -5.541024198820441 2210 22 -9.950962296773426 2210 23 -7.934352145898977 2210 24 -10.561374537451954 2210 25 -8.213325103985675 2211 1 0.012637080116139288 2211 2 4.510598526949231 2211 3 0.44280355728863796 2211 4 1.4576632777283969 2211 5 0.8116543245744801 2211 6 0.07602686790679414 2211 7 -19.74197208495504 2211 8 -13.01602995748296 2211 9 -8.993116239863108 2211 10 -7.0973626693335845 2211 11 -9.475883269060537 2211 12 -4.733480589231242 2211 13 -6.640243501776677 2211 14 -4.701095060536609 2211 15 -5.2906479996648414 2211 16 -10.912333858459423 2211 17 -7.638939868726805 2211 18 -5.914327082623672 2211 19 -8.297433148890537 2211 20 -6.668180120854963 2211 21 -4.504605722172844 2211 22 -5.9625630293188685 2211 23 -8.229155148530303 2211 24 -20.928200693751794 2211 25 -20.734856204464144 2212 1 -1.7624853840422539 2212 2 -13.29145194623689 2212 3 -7.973883626933361 2212 4 -18.849907700189583 2212 5 -19.35474946091448 2212 6 -19.584856412850304 2212 7 -9.959045255686748 2212 8 -9.572328846970278 2212 9 -5.774196489151404 2212 10 -6.080463897822209 2212 11 -5.839047845501252 2212 12 -3.437104502441983 2212 13 -5.932809735009988 2212 14 -4.140890141452719 2212 15 -4.39623903349316 2212 16 -4.469127733969156 2212 17 -4.530110908197454 2212 18 -3.6672085168007875 2212 19 -4.266842756235821 2212 20 -4.291105924245489 2212 21 -2.3030617761340118 2212 22 -3.4716920590949734 2212 23 -3.547624795371881 2212 24 -2.5834852465973235 2212 25 -2.935669212594224 2213 1 -18.00313579054632 2213 2 -13.29145194623689 2213 3 -8.215490084290574 2213 4 -5.085096210063909 2213 5 -19.35474946091448 2213 6 -9.855951565453536 2213 7 -19.74197208495504 2213 8 -9.92546338011094 2213 9 -7.796060131486211 2213 10 -6.616332590440912 2213 11 -5.542892483588567 2213 12 -4.474915948779067 2213 13 -18.3019154260669 2213 14 -11.51877398492311 2213 15 -6.449229064291135 2213 16 -5.612605423683217 2213 17 -4.1380804313397235 2213 18 -4.197063576572212 2213 19 -5.084959554998061 2213 20 -5.105216067142248 2213 21 -3.419647433911596 2213 22 -4.095399201013787 2213 23 -4.040185114152809 2213 24 -3.0727249307356352 2213 25 -4.027495342704718 2214 1 -18.00313579054632 2214 2 -13.29145194623689 2214 3 -11.57080551744389 2214 4 -18.849907700189583 2214 5 -19.35474946091448 2214 6 -19.584856412850304 2214 7 -15.505846958819824 2214 8 -13.928627403901357 2214 9 -12.7370179801312 2214 10 -12.578201257637785 2214 11 -13.530554330697287 2214 12 -11.088589821533317 2214 13 -18.3019154260669 2214 14 -15.671023947331644 2214 15 -22.71493822048503 2214 16 -17.044742412416646 2214 17 -22.398403591124282 2214 18 -16.61630895735111 2214 19 -13.77076012638833 2214 20 -12.27487857731006 2214 21 -9.951574816259368 2214 22 -11.038200390172229 2214 23 -10.915750118401096 2214 24 -9.817201702833529 2214 25 -9.593584761477262 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 150605.0 3 6.0 4 828.0 5 2925.0 6 3809.0 7 3567.0 8 2952.0 9 2837.0 10 2603.0 11 2445.0 12 2435.0 13 2484.0 14 3686.0 15 7468.0 16 10711.0 17 11576.0 18 11897.0 19 14238.0 20 16677.0 21 19360.0 22 19955.0 23 21814.0 24 22304.0 25 22850.0 26 19411.0 27 15903.0 28 16848.0 29 19220.0 30 19091.0 31 21462.0 32 27333.0 33 33554.0 34 39902.0 35 44611.0 36 42518.0 37 42188.0 38 28536.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.789754710677116 17.933038725579813 12.627104753859635 25.65010180988344 2 13.173787875204521 20.28087798018976 41.06034447542855 25.484989669177168 3 19.892261067528967 24.50284935400142 29.024984411931037 26.57990516653858 4 11.378732011679924 16.89134563255161 37.27699872865101 34.45292362711746 5 12.204447382027997 37.529035430736364 36.63611193247493 13.630405254760703 6 31.061853453118026 36.294409726568645 18.180023687327278 14.463713132986047 7 26.65455406981745 31.058851224815935 22.61997280701887 19.666621898347746 8 25.522556335207465 37.554184159385244 20.30411369401712 16.619145811390172 9 26.32763794308264 15.829028677911294 19.46576600030144 38.37756737870463 10 14.581845663970569 30.542118292984497 33.87760035342856 20.998435689616368 11 33.244973187432755 22.71484131426533 23.726584319651046 20.313601178650874 12 23.571492885420266 25.16693630134798 31.599538764947297 19.662032048284455 13 28.47802261331492 21.078618969063434 24.668967190662848 25.774391226958798 14 20.55601163227539 23.101337155101643 27.410149021135677 28.93250219148729 15 24.063435929541953 30.093712809579404 23.490809207149585 22.352042053729054 16 21.38861323595217 28.34141941421673 28.713092110742373 21.556875239088733 17 21.73232120327885 27.881222732046325 28.93958936840547 21.446866696269357 18 21.506119226213976 27.69012416385403 30.51411508927231 20.28964152065969 19 22.86116410026273 27.119176268393563 27.115008148674054 22.904651482669653 20 22.94032219098656 28.374412501098128 27.79363963805675 20.89162566985856 21 21.86722733546445 26.42351948395586 26.897513462487392 24.811739718092298 22 22.07597191585891 28.158178566706795 27.445637425110114 22.32021209232418 23 22.090805454465734 27.683660718200553 27.645158284737143 22.58037554259657 24 22.28511252459451 28.474040550434943 27.4571159113302 21.78373101364035 25 22.905179501233214 26.9906823787339 27.395450808440668 22.708687311592218 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 917.0 1 917.0 2 1814.5 3 2712.0 4 2712.0 5 2712.0 6 3741.5 7 4771.0 8 4771.0 9 4771.0 10 5549.5 11 6328.0 12 6328.0 13 6328.0 14 7500.0 15 8672.0 16 8672.0 17 8672.0 18 11931.0 19 15190.0 20 15190.0 21 15190.0 22 21124.5 23 27059.0 24 27059.0 25 27059.0 26 35411.5 27 43764.0 28 43764.0 29 43764.0 30 53949.0 31 64134.0 32 64134.0 33 64134.0 34 70093.0 35 76052.0 36 76052.0 37 76052.0 38 79723.0 39 83394.0 40 83394.0 41 83394.0 42 86110.5 43 88827.0 44 88827.0 45 88827.0 46 91247.5 47 93668.0 48 93668.0 49 93668.0 50 86039.0 51 78410.0 52 78410.0 53 78410.0 54 66871.0 55 55332.0 56 55332.0 57 55332.0 58 46290.0 59 37248.0 60 37248.0 61 37248.0 62 29953.0 63 22658.0 64 22658.0 65 22658.0 66 17466.0 67 12274.0 68 12274.0 69 12274.0 70 8917.0 71 5560.0 72 5560.0 73 5560.0 74 3935.5 75 2311.0 76 2311.0 77 2311.0 78 1594.5 79 878.0 80 878.0 81 878.0 82 605.5 83 333.0 84 333.0 85 333.0 86 207.0 87 81.0 88 81.0 89 81.0 90 55.0 91 29.0 92 29.0 93 29.0 94 17.5 95 6.0 96 6.0 97 6.0 98 3.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.152452269271253 2 36.33899938270675 3 5.608882452857821 4 13.657510378328217 5 13.439746841333736 6 14.712520650580544 7 9.298817835531729 8 7.7358751397806484 9 0.10662337857869257 10 1.4786294721253093 11 5.612714872113538 12 5.751776942249548 13 15.951897663456101 14 1.631378753888879 15 2.9319376027396324 16 3.037192260155569 17 2.903605074670583 18 1.5380319705889196 19 1.4864311827530183 20 0.28702082782993366 21 4.303806824169974 22 1.6498564895860852 23 0.10744461127634618 24 9.148942868209947 25 0.11072954206696058 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 730609.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.8633944571417 #Duplication Level Percentage of deduplicated Percentage of total 1 96.54856164211569 86.76181479115101 2 2.4297489911024015 4.366909840385544 3 0.46694273449106344 1.2588317741540045 4 0.18302777818951402 0.6578998971223413 5 0.09284326004850116 0.41716052502127215 6 0.05489757133026607 0.29599692643144637 7 0.036434411857661525 0.22918839472052988 8 0.02840796106125479 0.20422686484565283 9 0.022597387916558142 0.18276101856420404 >10 0.11368977060320158 1.8632672919375912 >50 0.011900485202711178 0.7712572952031163 >100 0.009678672044657484 1.9174606440143418 >500 9.520005274295956E-4 0.5412463264993483 >1k 3.173335091431985E-4 0.5319784099497019 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 1891 0.25882517187716003 No Hit GTACATGGGAAGCAGTGGTATCAAC 1840 0.2518446939471044 No Hit GAGTACATGGGAAGCAGTGGTATCA 881 0.12058433443880379 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.3687211627560022E-4 0.0 11 0.0 0.0 0.0 1.3687211627560022E-4 0.0 12 0.0 0.0 0.0 1.3687211627560022E-4 0.0 13 0.0 0.0 0.0 1.3687211627560022E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTTAG 35 0.005903771 19.066866 1 AGACCCG 45 3.296034E-4 16.23995 5 TGTAGAA 260 0.0 15.803825 2 CCAGTAT 50 5.0655263E-4 15.347837 3 GTCCTAC 460 0.0 15.087695 1 TTAGAAG 45 0.0044536158 14.616987 3 TCCTACA 495 0.0 14.1117 2 TGTAGGA 370 0.0 14.066829 2 GTATTAA 105 9.249816E-7 13.98237 1 TAGACTG 75 5.571408E-6 13.919957 5 GTAGGAG 65 2.3941259E-4 13.492605 3 GGCGAGG 185 0.0 13.354824 19 GTGTTTA 60 0.008131321 13.3468075 1 CCTTAGT 60 0.008131321 13.3468075 1 CCTACAG 495 0.0 13.288171 3 TCCAACG 155 0.0 13.199538 18 TAGATCA 75 6.546937E-5 12.896215 4 CCCTGAA 85 3.5185285E-4 12.8909645 2 GTCCTAA 105 1.4201709E-5 12.711246 1 CTACACT 110 9.96115E-8 12.70082 4 >>END_MODULE