FastQCFastQC Report
Thu 26 May 2016
SRR1926567_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926567_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730609
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC54380.7443105683067139No Hit
GTACATGGGAAGCAGTGGTATCAAC51460.7043439103542387No Hit
GAGTACATGGGAAGCAGTGGTATCA23580.32274445017786535No Hit
CATGTACTCTGCGTTGATACCACTG19620.2685430921327276No Hit
GTATCAACGCAGAGTACTTTTTTTT16310.22323842164550398No Hit
GCGTTGATACCACTGCTTCCCATGT15700.21488922255269235No Hit
GATATACACTGTTCTACAAATCCCG15660.21434173408758994No Hit
GTATCAACGCAGAGTACATGGGAAG14790.2024338599716127No Hit
ACGCAGAGTACATGGGAAGCAGTGG14150.1936740445299743No Hit
GTCCTACAGTGGACATTTCTAAATT11810.16164596932148387No Hit
CTGTAGGACGTGGAATATGGCAAGA11660.15959288757734985No Hit
ACTCTGCGTTGATACCACTGCTTCC11450.15671857313556226No Hit
GTGTATATCAATGAGTTACAATGAA11030.15096994425198704No Hit
GCTTCCCATGTACTCTGCGTTGATA11010.15069620001943584No Hit
GGTATCAACGCAGAGTACTTTTTTT10080.13796709320580502No Hit
CTTTAGGACGTGAAATATGGCGAGG9190.1257854748572766No Hit
GATATACACTGTTCTACAATGCCGG9000.12318490464804019No Hit
GGTATCAACGCAGAGTACATGGGAA8900.1218161834852842No Hit
TATCAACGCAGAGTACATGGGAAGC8830.120858078671355No Hit
GTCCTAAAGTGTGTATTTCTCATTT8700.11907874115977218No Hit
TATCAACGCAGAGTACTTTTTTTTT8230.11264575169481897No Hit
GTGTATATCAATGAGTTACAATGAG8170.11182451899716538No Hit
GTCCTACAGTGTGCATTTCTCATTT8010.10963456513675579No Hit
GTTCTACAGTGTGGTTTTTATCATT7940.10867646032282657No Hit
TTGTAGAACAGTGTATATCAATGAG7480.10238034297414896No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGTG400.005262658514.2552529
CTAAACT551.8934465E-413.8728044
TCCAACG1750.013.53276218
CCAACGA1400.013.53276219
TAGGACC3050.012.8209724
GTAGGAC7650.012.718493
GTCCTAC6750.012.7088551
GATATAC4550.012.3597111
CTAGACT853.7653354E-612.3427154
CCTACAC1650.012.1403763
TGTAGGA8100.012.0119072
GACGTGG3650.011.9753717
ACTGTTC5400.011.9673728
TATACAC5450.011.9017063
GGACGTG5950.011.8610136
CTGTAGG7650.011.83667851
GCTCTAG657.7939074E-411.7312521
CGTGGAA3750.011.6576299
GGCGAGG2550.011.51611519
TCCTACA7750.011.4466422
Produced by FastQC (version 0.11.5)