FastQCFastQC Report
Thu 26 May 2016
SRR1926566_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926566_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550142
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC26560.48278444474335713No Hit
CCCATGTACTCTGCGTTGATACCAC26440.4806031897219263No Hit
GAGTACATGGGAAGCAGTGGTATCA12420.22575989471809096No Hit
CATGTACTCTGCGTTGATACCACTG9980.18140770928233074No Hit
GCGTTGATACCACTGCTTCCCATGT9860.17922645426089992No Hit
GCTTCCCATGTACTCTGCGTTGATA7800.14178157639300398No Hit
GTATCAACGCAGAGTACATGGGAAG7550.13723729509835642No Hit
ACGCAGAGTACATGGGAAGCAGTGG7220.13123884378942163No Hit
CNCATGTACTCTGCGTTGATACCAC6550.11906016991976617No Hit
GTCCTACAGTGGACATTTCTAAATT6060.11015337858225693No Hit
ACTCTGCGTTGATACCACTGCTTCC5550.10088304474117592No Hit
GNACATGGGAAGCAGTGGTATCAAC5540.10070127348939001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC1200.019.9927061
TCGTTGA300.00106558218.10709413
GTATTAG552.3598775E-416.9635091
GTCCTAG552.3598775E-416.9635091
TGTAGGA3150.016.8896262
GTCTATA500.002442534515.9941651
GTCTTAA500.002442534515.9941651
GGCCTAT500.002442534515.9941651
GTACTGT400.002900223215.5890926
TCTCCGT551.520984E-515.05784110
TAGAAGT506.103445E-414.9741974
CCAACGA751.3897079E-814.757956519
CCTAGAG450.00456187314.5629433
GTAGGAC4050.014.5629433
TAGGAGA450.00524250314.2611414
TAGGACC1201.10958354E-1014.2611414
AGGACGT3750.014.144665
CTGTAGG3550.013.8916461
AGGACGG450.006312356313.8673145
CTCCGTT400.006506637713.8045411
Produced by FastQC (version 0.11.5)