##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926566_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 550142 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.52154898189922 27.0 10.0 31.0 2.0 31.0 2 16.66021499903661 22.0 2.0 30.0 2.0 31.0 3 20.376337381985014 25.0 15.0 31.0 2.0 31.0 4 22.386531113785168 28.0 7.0 35.0 2.0 37.0 5 22.86242642808584 29.0 7.0 35.0 2.0 37.0 6 23.441186093772153 30.0 7.0 35.0 2.0 37.0 7 23.647240167084135 31.0 8.0 35.0 2.0 37.0 8 24.036070687204393 31.0 12.0 35.0 2.0 37.0 9 26.726367010699057 32.0 17.0 37.0 2.0 39.0 10 27.439650126694563 32.0 25.0 37.0 2.0 39.0 11 24.486876115621058 30.0 8.0 37.0 2.0 39.0 12 25.625994743175397 31.0 17.0 37.0 2.0 39.0 13 22.235850016904728 28.0 2.0 37.0 2.0 39.0 14 25.647520094811885 31.0 17.0 38.0 2.0 40.0 15 26.757026367737783 31.0 17.0 38.0 2.0 40.0 16 27.11363248034144 32.0 19.0 39.0 2.0 40.0 17 26.394107339559604 31.0 17.0 38.0 2.0 40.0 18 26.456749348350062 31.0 17.0 38.0 2.0 40.0 19 26.881701451625215 31.0 18.0 39.0 2.0 40.0 20 27.142377786098862 31.0 22.0 39.0 2.0 40.0 21 24.932233859621697 30.0 8.0 38.0 2.0 40.0 22 25.910686695434997 31.0 10.0 38.0 2.0 40.0 23 25.89574146311316 31.0 2.0 38.0 2.0 40.0 24 24.956331274470955 31.0 2.0 39.0 2.0 40.0 25 24.755055967368424 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.571689943299603 1101 2 10.315869995577383 1101 3 6.936024385810864 1101 4 8.256253846367969 1101 5 7.807933827864481 1101 6 7.020187128609624 1101 7 7.1211282066969765 1101 8 5.56999625304692 1101 9 5.474539947836618 1101 10 5.004228427404346 1101 11 7.694079185838696 1101 12 5.528913933421936 1101 13 10.251714971468804 1101 14 7.3374931587374554 1101 15 6.537120569358976 1101 16 6.164403563848829 1101 17 2.078691624792743 1101 18 4.396140542809544 1101 19 4.968380365026263 1101 20 2.8152471829884718 1101 21 -0.1320293489488833 1101 22 3.9557996392731702 1101 23 5.275367527429843 1101 24 6.673554256352492 1101 25 7.22504627018121 1102 1 6.0469244849669685 1102 2 11.238193028954953 1102 3 7.718781478754035 1102 4 8.87998349982583 1102 5 8.564936481943867 1102 6 7.11931405407703 1102 7 7.27687479250941 1102 8 7.770386595893505 1102 9 6.92817229904859 1102 10 6.056108960235981 1102 11 8.865869933516375 1102 12 6.035310810679164 1102 13 10.79961753483915 1102 14 8.39875640813693 1102 15 6.225058366473085 1102 16 6.64991006466353 1102 17 7.521458838513325 1102 18 7.496803432732438 1102 19 6.459903689008723 1102 20 6.545553661965808 1102 21 5.686177761632159 1102 22 6.810599029506832 1102 23 7.1808757920314825 1102 24 8.716466772520537 1102 25 8.40249058490799 1103 1 4.491298207449468 1103 2 8.950160315462558 1103 3 5.7562104804362875 1103 4 7.330117356526806 1103 5 6.686038357746558 1103 6 6.183038435894225 1103 7 6.508071543771102 1103 8 6.33162716713602 1103 9 5.16148656383162 1103 10 4.285374738142387 1103 11 5.018703987384654 1103 12 4.02437051257667 1103 13 8.008710972243055 1103 14 5.987028345260459 1103 15 5.1065115256721185 1103 16 4.945947047099359 1103 17 2.243735578198219 1103 18 1.2796288492257553 1103 19 3.2100823153495526 1103 20 3.619376084772096 1103 21 5.866573407671627 1103 22 1.299039191943745 1103 23 3.481287989271138 1103 24 5.039610867450264 1103 25 1.0270417579629978 1104 1 5.6045106728497665 1104 2 10.572922885930573 1104 3 7.249325794255977 1104 4 7.484066396281609 1104 5 7.31368735145745 1104 6 6.850182634521882 1104 7 7.051831953059793 1104 8 7.260943890476224 1104 9 5.903311633298095 1104 10 4.936387543904392 1104 11 7.9540576975515975 1104 12 4.957348314284463 1104 13 9.522250125056487 1104 14 6.959088986477983 1104 15 6.4243021017337725 1104 16 5.739104131611832 1104 17 6.392539665102625 1104 18 4.009758124712555 1104 19 4.6115617029548766 1104 20 3.805608208037306 1104 21 2.9253183853066815 1104 22 4.943622943251082 1104 23 5.4939399154769575 1104 24 6.358933391682072 1104 25 5.5786317684644295 1105 1 3.0248033483767394 1105 2 9.214451752783468 1105 3 5.782278487727115 1105 4 4.982426171809877 1105 5 1.3913322945061815 1105 6 3.949697902531664 1105 7 5.01391367585936 1105 8 2.385104940858607 1105 9 3.0984738942629484 1105 10 2.865181741891014 1105 11 6.017539923546288 1105 12 3.658129853550548 1105 13 4.662840366344636 1105 14 3.3947060591837896 1105 15 3.710975614728323 1105 16 4.11582482039633 1105 17 4.550995229340845 1105 18 1.8581568724133959 1105 19 1.3613244267953668 1105 20 0.43301627412931865 1105 21 2.0872869341147116 1105 22 3.0936872648009484 1105 23 0.01157120579020443 1105 24 3.346950321872484 1105 25 4.4289736357554474 1106 1 6.408496297171027 1106 2 11.41210066192967 1106 3 8.00098465943903 1106 4 9.255035786204601 1106 5 8.959859683710729 1106 6 7.784386334767042 1106 7 7.724969058575969 1106 8 7.635442772378855 1106 9 6.541137020383111 1106 10 5.859420152030779 1106 11 8.636539784772086 1106 12 5.586759561662145 1106 13 4.8984021902510015 1106 14 6.125657331723524 1106 15 6.399985583833839 1106 16 6.279062648865512 1106 17 6.8212250138274655 1106 18 6.669979056404369 1106 19 6.690576551959854 1106 20 6.368309112297933 1106 21 6.570521337518517 1106 22 6.704531927259772 1106 23 7.286284085906029 1106 24 8.060249712939164 1106 25 7.150324883099302 1107 1 3.65185859881041 1107 2 -14.063713750706064 1107 3 -5.974539689725113 1107 4 1.8018263527698863 1107 5 4.402617321890311 1107 6 4.451034931905969 1107 7 4.394498318847546 1107 8 5.177082572510859 1107 9 3.2381291097340714 1107 10 2.490010531053393 1107 11 5.357293303099343 1107 12 2.539109265987612 1107 13 4.424097058695327 1107 14 3.1876614896169606 1107 15 3.009034232708675 1107 16 2.7042253221665504 1107 17 3.2726712417659947 1107 18 -1.902136428800496 1107 19 0.7783411992141396 1107 20 1.2847318403401289 1107 21 -2.243341604162559 1107 22 0.8834269090666105 1107 23 -2.5149273332746844 1107 24 1.211115053112291 1107 25 2.0041671304159756 1108 1 5.501989970329063 1108 2 11.083760996768682 1108 3 0.692769237699423 1108 4 6.446264958480171 1108 5 7.999318437088537 1108 6 8.136969421308667 1108 7 8.231702732930572 1108 8 7.577660607192939 1108 9 6.086713216699678 1108 10 5.359310466827317 1108 11 7.468690512184413 1108 12 2.5182182751544246 1108 13 3.8222228250291863 1108 14 5.280474007863003 1108 15 5.289503666935335 1108 16 5.299291591799296 1108 17 0.6545653271282887 1108 18 4.01493602989046 1108 19 5.236786928180102 1108 20 5.496362598791695 1108 21 5.507618867607164 1108 22 2.5661909658191036 1108 23 5.1288748504118935 1108 24 6.004178636927318 1108 25 6.4271075169401755 1109 1 3.658428071477065 1109 2 -14.063713750706064 1109 3 -5.734231141519388 1109 4 2.5777573568667655 1109 5 1.0664332714388642 1109 6 2.544819250660204 1109 7 4.183755685666117 1109 8 4.362188479775419 1109 9 3.5581097761508467 1109 10 3.0769820633385017 1109 11 1.0039095972328482 1109 12 1.7599461339471283 1109 13 2.7857764500162574 1109 14 3.8524704225215345 1109 15 4.154670695583594 1109 16 3.7965267419354696 1109 17 4.282268984866075 1109 18 4.420089958252582 1109 19 2.514450940315104 1109 20 1.4373389148533349 1109 21 0.5468955939146056 1109 22 2.9397145980458106 1109 23 3.4284956316026403 1109 24 4.574599569805589 1109 25 4.871252033780941 1110 1 3.6432487115878054 1110 2 9.974747787755476 1110 3 6.7735773185075026 1110 4 8.04509945731467 1110 5 8.69224773001783 1110 6 4.3117945961338435 1110 7 7.055478956706796 1110 8 7.890248019780351 1110 9 6.36822059820706 1110 10 5.427686535203385 1110 11 3.5138342573281562 1110 12 6.290790147726295 1110 13 10.922689025495387 1110 14 8.396946424335422 1110 15 7.469690147121817 1110 16 6.920348312856014 1110 17 7.590307362870327 1110 18 7.808098423052858 1110 19 6.719459810852985 1110 20 6.825733228162324 1110 21 4.329141789130084 1110 22 6.695794695422833 1110 23 7.4362563577934 1110 24 8.361754394503077 1110 25 8.630526320358978 1111 1 5.3523368122421715 1111 2 9.884077413952571 1111 3 7.602073119894868 1111 4 8.326294166220222 1111 5 8.744000194420895 1111 6 7.95423849761389 1111 7 7.576956475172267 1111 8 7.700552173458 1111 9 5.98783893168082 1111 10 5.427918231218214 1111 11 7.598129480177597 1111 12 6.698399606540573 1111 13 8.516216983481176 1111 14 6.520391252599527 1111 15 6.094834079494667 1111 16 5.73494937444503 1111 17 7.12928846209239 1111 18 7.079077444031878 1111 19 5.8163719348735405 1111 20 5.622156684224333 1111 21 8.061020352333951 1111 22 7.553175266658826 1111 23 7.359263069956743 1111 24 7.319038074678321 1111 25 7.598039730643475 1112 1 -2.249928807614303 1112 2 7.688335429621805 1112 3 5.851353026406162 1112 4 -0.6833674309882838 1112 5 3.9747551815826583 1112 6 2.8384721535491053 1112 7 5.9925944368550645 1112 8 6.164819077138294 1112 9 4.585125619415361 1112 10 3.4083769513446214 1112 11 -0.49867577362449467 1112 12 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4.1315479452680925 2204 7 5.391148184296274 2204 8 5.270770086031693 2204 9 3.759451088598098 2204 10 3.541133948231071 2204 11 5.0088995278393895 2204 12 5.220029673503074 2204 13 2.837949875173866 2204 14 4.191906273859804 2204 15 4.317654005578856 2204 16 4.654335207388556 2204 17 -1.032125293665164 2204 18 2.9117986752315943 2204 19 3.744680130931645 2204 20 4.0067222286686395 2204 21 5.623926752330576 2204 22 5.245913691501958 2204 23 4.230437168238641 2204 24 5.6188690881340335 2204 25 5.567857090323539 2205 1 5.239115004729577 2205 2 9.59824386786104 2205 3 6.536810049449617 2205 4 6.972754882951932 2205 5 6.945618980361832 2205 6 6.690114392718019 2205 7 6.68963625584896 2205 8 6.588827778400475 2205 9 5.259017490517575 2205 10 4.641573052318964 2205 11 7.056635552904428 2205 12 6.194716863459266 2205 13 9.676615388402578 2205 14 6.809898423563908 2205 15 5.433316161201919 2205 16 5.122856198512235 2205 17 6.040509532043231 2205 18 5.7646643932414285 2205 19 4.185704968319129 2205 20 4.524497632274862 2205 21 -1.7567289496165372 2205 22 2.8800215012439914 2205 23 4.342205941341369 2205 24 6.388235145504513 2205 25 6.549584557782506 2206 1 4.191913839563277 2206 2 8.742197579343198 2206 3 6.405193977710368 2206 4 1.7140362676307888 2206 5 5.846211008119038 2206 6 6.418907023935926 2206 7 6.4763362603227215 2206 8 6.34974599307143 2206 9 5.155519506195624 2206 10 4.2342420486554495 2206 11 4.060156900202525 2206 12 5.168713373925385 2206 13 8.401115197025007 2206 14 0.4927793270648735 2206 15 2.6313308053141995 2206 16 3.6902404896447436 2206 17 4.6937453042393 2206 18 -0.9185416035871725 2206 19 1.167274065563788 2206 20 2.811678317555689 2206 21 3.7980452994129053 2206 22 -1.6986843887114205 2206 23 -2.1437578808415267 2206 24 1.696789474365744 2206 25 3.2530865187812488 2207 1 1.305524311739159 2207 2 -14.063713750706064 2207 3 -6.414890526272968 2207 4 -4.454524499223115 2207 5 1.0617139351553746 2207 6 1.81265412404748 2207 7 3.1069501975567952 2207 8 3.272583786885658 2207 9 0.8582846423793207 2207 10 0.2999695627970347 2207 11 -1.589196979971618 2207 12 -2.582702758832742 2207 13 0.21863990160658275 2207 14 -5.690190553583122 2207 15 -1.6151602062656103 2207 16 -0.44096872681773647 2207 17 0.6974166864125131 2207 18 0.7582691607987435 2207 19 0.0634625015490613 2207 20 -0.05167893882899932 2207 21 -3.401949764606762 2207 22 -4.203299530324699 2207 23 -1.058210431262566 2207 24 0.5420543884303264 2207 25 1.078408097900077 2208 1 4.8452612659625665 2208 2 6.8083334933884245 2208 3 6.562410532537566 2208 4 8.496008456452014 2208 5 3.9455583957300675 2208 6 6.627454985613131 2208 7 7.164886937177769 2208 8 7.132516250237558 2208 9 5.6050123497625925 2208 10 5.0569263898369385 2208 11 7.023800668869374 2208 12 6.7482011750192115 2208 13 9.261473600500434 2208 14 3.212707762734553 2208 15 5.0980398158258176 2208 16 5.902604245899347 2208 17 6.428422004134575 2208 18 1.058736236682794 2208 19 3.8488166744618155 2208 20 5.021881568011452 2208 21 7.72003436112502 2208 22 6.890164957706482 2208 23 2.337230056011194 2208 24 3.85713139283283 2208 25 5.445555779089226 2209 1 3.0347838779920835 2209 2 4.902778395890795 2209 3 5.293234624552245 2209 4 -0.11112041356488689 2209 5 4.9506960879426245 2209 6 5.451280067032926 2209 7 5.923405096884245 2209 8 4.439091671765365 2209 9 4.340772130444456 2209 10 3.2584960398348777 2209 11 -0.267302930091752 2209 12 2.96496849991512 2209 13 2.129441609734883 2209 14 3.8646772973018955 2209 15 4.0987849615569445 2209 16 3.746057682020883 2209 17 0.966110094170439 2209 18 3.318257199703776 2209 19 4.15592596250238 2209 20 4.2242540790391985 2209 21 6.247832522480504 2209 22 5.131413875544634 2209 23 0.4876299405805966 2209 24 -2.753936546318755 2209 25 0.35578169325833287 2210 1 -2.567965557646943 2210 2 -14.063713750706064 2210 3 -7.95266117564908 2210 4 -2.2959627796520046 2210 5 -0.948356262122001 2210 6 -1.3755447993966783 2210 7 -1.1689178069391133 2210 8 -21.355672726140394 2210 9 -13.452129131698983 2210 10 -7.563518505489711 2210 11 -10.196317934052702 2210 12 -5.020486175495421 2210 13 -5.5509698526003515 2210 14 -4.265845845965384 2210 15 -6.1559095091945615 2210 16 -5.359332188667491 2210 17 -4.803055247215834 2210 18 -5.092993090666646 2210 19 -10.782477387329948 2210 20 -7.15310477115349 2210 21 -5.276767659782777 2210 22 -9.844130680680015 2210 23 -7.796760341093606 2210 24 -10.856572216315001 2210 25 -8.055937954842495 2211 1 0.48530687034878284 2211 2 5.373379524782006 2211 3 0.9734392786731512 2211 4 2.2552638410148447 2211 5 1.397039684267483 2211 6 0.9001653721401937 2211 7 -20.956176054948216 2211 8 -13.143091381238008 2211 9 -8.312679846250866 2211 10 -6.273131085353931 2211 11 -9.372351140787826 2211 12 -4.061919326458231 2211 13 -6.292534290595608 2211 14 -3.8939864316733583 2211 15 -4.315958766509745 2211 16 -11.245580118799097 2211 17 -7.037417406936875 2211 18 -5.089939186481502 2211 19 -8.150077376471621 2211 20 -6.0220361391298205 2211 21 -3.824408565071028 2211 22 -5.378941486688618 2211 23 -7.366738550407582 2211 24 -22.39582136307268 2211 25 -22.19814500831235 2212 1 -1.0105893108548685 2212 2 -14.063713750706064 2212 3 -7.977902596925901 2212 4 -19.80221989000468 2212 5 -20.21684317907308 2212 6 -20.696363912024665 2212 7 -9.994548147971472 2212 8 -9.65450993544272 2212 9 -5.019053755346363 2212 10 -5.63358359002023 2212 11 -5.870797390094186 2212 12 -3.1030760635817742 2212 13 -5.9270724533358585 2212 14 -3.936674218587548 2212 15 -3.860919166045637 2212 16 -4.038880830094058 2212 17 -4.187518299839059 2212 18 -3.167046586975875 2212 19 -3.9435748289258434 2212 20 -4.003214568227332 2212 21 -1.9436261406145903 2212 22 -3.4510042420737754 2212 23 -3.419316364058389 2212 24 -2.4737283398168657 2212 25 -2.7714008222658357 2213 1 -19.007100938761845 2213 2 -14.063713750706064 2213 3 -8.91996393342791 2213 4 -6.080808697790568 2213 5 -20.21684317907308 2213 6 -10.713395542194982 2213 7 -20.956176054948216 2213 8 -11.119662993780297 2213 9 -8.702330505190758 2213 10 -7.546204537227833 2213 11 -7.257336178075853 2213 12 -5.623992150267682 2213 13 -19.47474687194051 2213 14 -12.804455579986291 2213 15 -7.539029843349997 2213 16 -6.705117462244797 2213 17 -5.428255582699919 2213 18 -5.37530211801192 2213 19 -6.178622641973995 2213 20 -6.222792455399855 2213 21 -4.423355105702576 2213 22 -5.207553780918342 2213 23 -5.057337639450232 2213 24 -4.040590219520368 2213 25 -4.765054983981447 2214 1 -19.007100938761845 2214 2 -14.063713750706064 2214 3 -12.776955255185834 2214 4 -19.80221989000468 2214 5 -20.21684317907308 2214 6 -20.696363912024665 2214 7 -16.874432185738407 2214 8 -15.51779806673985 2214 9 -14.451304563279953 2214 10 -14.481137610677981 2214 11 -14.909109284143995 2214 12 -13.017184801938027 2214 13 -19.47474687194051 2214 14 -17.27246409248654 2214 15 -24.192314514882845 2214 16 -18.72257007665638 2214 17 -23.87472760216464 2214 18 -18.353029731263312 2214 19 -15.67426363191678 2214 20 -14.356151644458242 2214 21 -11.800305058300415 2214 22 -12.780609463542632 2214 23 -12.73930559161051 2214 24 -11.662851335824724 2214 25 -11.439289422481286 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 93159.0 3 3.0 4 368.0 5 1545.0 6 2126.0 7 2119.0 8 1966.0 9 1792.0 10 1725.0 11 1551.0 12 1625.0 13 1703.0 14 2639.0 15 5573.0 16 8294.0 17 8988.0 18 9109.0 19 10452.0 20 12238.0 21 14370.0 22 14973.0 23 16258.0 24 17218.0 25 17221.0 26 14782.0 27 11562.0 28 12466.0 29 14715.0 30 14293.0 31 16722.0 32 21518.0 33 27161.0 34 33091.0 35 37783.0 36 36430.0 37 37076.0 38 25528.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.17691796933092 17.982317419289974 12.304495631719726 25.536268979659376 2 13.021785561725757 20.6888793962694 40.70140965399402 25.587925388010824 3 19.986773940421035 24.941533490272292 28.519510365875995 26.55218220343068 4 12.254048159812756 17.17024857287528 36.69091955785922 33.88478370945274 5 12.339792669164257 37.756698816510955 35.50697885158351 14.396529662741282 6 31.95029306927324 35.21361304647424 18.02758849501059 14.808505389241933 7 27.33925384005827 31.140291196081098 22.12565935341343 19.3947956104472 8 25.182852239682223 38.317386327266725 19.956567070692394 16.54319436235865 9 26.669384071157175 16.210530912902332 19.544021952746277 37.57606306319421 10 15.487559711540236 30.17023552629152 33.155720319445216 21.18648444272303 11 32.38357305524435 23.300236990128948 23.454309498695494 20.861880455931214 12 24.19040134674687 25.89328880131439 30.57589222943235 19.34041762250639 13 28.478095600114035 21.355386037510907 24.2440800670393 25.92243829533576 14 20.72720288399031 23.70224129779564 26.71994119732876 28.85061462088529 15 24.919759474932203 30.031197890446183 22.632974691717305 22.416067942904306 16 20.891397284932122 28.316582914572862 29.265919147978696 21.526100652516313 17 22.37726649341124 28.67452116204621 28.490600986770097 20.45761135777245 18 22.365669975437953 26.81064342960695 30.62671043914574 20.196976155809356 19 23.193738909732136 27.632428404366383 27.1332971804435 22.04053550545798 20 23.439234753312952 27.903173761095584 27.73271786906435 20.924873616527112 21 21.342146133564487 26.691607119434735 27.1014517548612 24.864794992139576 22 22.426535101896818 28.735216049325707 26.10305865924368 22.735190189533803 23 21.048340870008282 28.415478540948257 27.852275105316316 22.683905483727152 24 22.29319712541658 29.116239319652635 27.204509557650415 21.386053997280367 25 22.558842904821354 27.049174361344754 26.808416164101367 23.58356656973253 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 931.0 1 931.0 2 1605.0 3 2279.0 4 2279.0 5 2279.0 6 2976.0 7 3673.0 8 3673.0 9 3673.0 10 4267.0 11 4861.0 12 4861.0 13 4861.0 14 5664.0 15 6467.0 16 6467.0 17 6467.0 18 8801.0 19 11135.0 20 11135.0 21 11135.0 22 15099.0 23 19063.0 24 19063.0 25 19063.0 26 25486.0 27 31909.0 28 31909.0 29 31909.0 30 38675.0 31 45441.0 32 45441.0 33 45441.0 34 50285.5 35 55130.0 36 55130.0 37 55130.0 38 58019.5 39 60909.0 40 60909.0 41 60909.0 42 64040.5 43 67172.0 44 67172.0 45 67172.0 46 72798.0 47 78424.0 48 78424.0 49 78424.0 50 70524.0 51 62624.0 52 62624.0 53 62624.0 54 51927.5 55 41231.0 56 41231.0 57 41231.0 58 34066.0 59 26901.0 60 26901.0 61 26901.0 62 21723.5 63 16546.0 64 16546.0 65 16546.0 66 12734.0 67 8922.0 68 8922.0 69 8922.0 70 6497.0 71 4072.0 72 4072.0 73 4072.0 74 2828.0 75 1584.0 76 1584.0 77 1584.0 78 1092.5 79 601.0 80 601.0 81 601.0 82 400.0 83 199.0 84 199.0 85 199.0 86 127.0 87 55.0 88 55.0 89 55.0 90 33.0 91 11.0 92 11.0 93 11.0 94 6.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.21895074362619 2 36.171024935380316 3 5.720159522450567 4 13.48470031373718 5 13.344918221113822 6 14.531884495275765 7 9.163088802527348 8 7.425537406705905 9 0.10869920856796972 10 1.445444994201497 11 5.735791850104155 12 5.628546811550472 13 15.837729168105689 14 1.5759567529837752 15 2.8739852619869053 16 3.057755997542453 17 2.848537286736879 18 1.4994310559819102 19 1.4541700142872203 20 0.2931970291306608 21 4.378687684270607 22 1.5924979368962922 23 0.11033514983404284 24 8.968411791864646 25 0.11415234612154679 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 550142.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.18251319345275 #Duplication Level Percentage of deduplicated Percentage of total 1 96.33571678024082 83.98789899194072 2 2.4631170117614456 4.294814627498202 3 0.5202980488296932 1.3608267451986733 4 0.20911544722273215 0.7292484094580246 5 0.12860262520813204 0.5605950034460315 6 0.07292632655384058 0.381474025615815 7 0.04601555990390566 0.28082265108984594 8 0.03657785019423518 0.2551159125717637 9 0.023008768466826188 0.18053660343817565 >10 0.13463641043639404 2.189211105846477 >50 0.013890196930567533 0.8572983607892574 >100 0.013228745960078604 2.2939441698814016 >500 0.0022047909933419546 1.3707152568633103 >1k 6.614372980025863E-4 1.25749813636233 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2656 0.48278444474335713 No Hit CCCATGTACTCTGCGTTGATACCAC 2644 0.4806031897219263 No Hit GAGTACATGGGAAGCAGTGGTATCA 1242 0.22575989471809096 No Hit CATGTACTCTGCGTTGATACCACTG 998 0.18140770928233074 No Hit GCGTTGATACCACTGCTTCCCATGT 986 0.17922645426089992 No Hit GCTTCCCATGTACTCTGCGTTGATA 780 0.14178157639300398 No Hit GTATCAACGCAGAGTACATGGGAAG 755 0.13723729509835642 No Hit ACGCAGAGTACATGGGAAGCAGTGG 722 0.13123884378942163 No Hit CNCATGTACTCTGCGTTGATACCAC 655 0.11906016991976617 No Hit GTCCTACAGTGGACATTTCTAAATT 606 0.11015337858225693 No Hit ACTCTGCGTTGATACCACTGCTTCC 555 0.10088304474117592 No Hit GNACATGGGAAGCAGTGGTATCAAC 554 0.10070127348939001 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.8177125178590254E-4 0.0 8 0.0 0.0 0.0 1.8177125178590254E-4 0.0 9 1.8177125178590254E-4 0.0 0.0 1.8177125178590254E-4 0.0 10 1.8177125178590254E-4 0.0 0.0 1.8177125178590254E-4 0.0 11 1.8177125178590254E-4 0.0 0.0 1.8177125178590254E-4 0.0 12 1.8177125178590254E-4 0.0 0.0 1.8177125178590254E-4 0.0 13 1.8177125178590254E-4 0.0 0.0 1.8177125178590254E-4 1.8177125178590254E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATATAC 120 0.0 19.992706 1 TCGTTGA 30 0.001065582 18.107094 13 GTATTAG 55 2.3598775E-4 16.963509 1 GTCCTAG 55 2.3598775E-4 16.963509 1 TGTAGGA 315 0.0 16.889626 2 GTCTATA 50 0.0024425345 15.994165 1 GTCTTAA 50 0.0024425345 15.994165 1 GGCCTAT 50 0.0024425345 15.994165 1 GTACTGT 40 0.0029002232 15.589092 6 TCTCCGT 55 1.520984E-5 15.057841 10 TAGAAGT 50 6.103445E-4 14.974197 4 CCAACGA 75 1.3897079E-8 14.7579565 19 CCTAGAG 45 0.004561873 14.562943 3 GTAGGAC 405 0.0 14.562943 3 TAGGAGA 45 0.005242503 14.261141 4 TAGGACC 120 1.10958354E-10 14.261141 4 AGGACGT 375 0.0 14.14466 5 CTGTAGG 355 0.0 13.891646 1 AGGACGG 45 0.0063123563 13.867314 5 CTCCGTT 40 0.0065066377 13.80454 11 >>END_MODULE