FastQCFastQC Report
Thu 26 May 2016
SRR1926566_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926566_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550142
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC69261.2589476898691612No Hit
GTACATGGGAAGCAGTGGTATCAAC68971.2536763235673698No Hit
GAGTACATGGGAAGCAGTGGTATCA32730.594937307095259No Hit
CATGTACTCTGCGTTGATACCACTG25960.47187816963620305No Hit
GCGTTGATACCACTGCTTCCCATGT21180.3849915112825416No Hit
ACGCAGAGTACATGGGAAGCAGTGG19870.3611794772985884No Hit
GTATCAACGCAGAGTACATGGGAAG19410.35281799971643685No Hit
GCTTCCCATGTACTCTGCGTTGATA16740.3042850754896009No Hit
ACTCTGCGTTGATACCACTGCTTCC16190.29428765664137624No Hit
GTATCAACGCAGAGTACTTTTTTTT15490.28156366901636304No Hit
TATCAACGCAGAGTACATGGGAAGC13950.25357089624133405No Hit
CTGTAGGACGTGGAATATGGCAAGA13720.24939015745025828No Hit
GTCCTACAGTGGACATTTCTAAATT12510.22739583598416407No Hit
GGTATCAACGCAGAGTACATGGGAA12280.22321509719308835No Hit
GGTATCAACGCAGAGTACTTTTTTT10460.19013272936805406No Hit
CAGTGGTATCAACGCAGAGTACATG9530.17322800295196514No Hit
CTTTAGGACGTGAAATATGGCGAGG8500.15450556401801718No Hit
TATCAACGCAGAGTACTTTTTTTTT8130.14778002770193877No Hit
GTCCTAAAGTGTGTATTTCTCATTT8120.14759825645015287No Hit
CATGGGAAGCAGTGGTATCAACGCA7950.14450814516979255No Hit
GTGGTATCAACGCAGAGTACATGGG7560.13741906635014234No Hit
GCAGTGGTATCAACGCAGAGTACAT7450.1354195825804974No Hit
ACATGGGAAGCAGTGGTATCAACGC7280.13232947130013706No Hit
ATACCACTGCTTCCCATGTACTCTG7040.1279669612572754No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6920.12578570623584456No Hit
GGGAAGCAGTGGTATCAACGCAGAG6780.12324090871084192No Hit
GATATACACTGTTCTACAAATCCCG6420.11669714364654943No Hit
AAGCAGTGGTATCAACGCAGAGTAC6330.11506120238047632No Hit
CTGCTTCCCATGTACTCTGCGTTGA6300.1145158886251186No Hit
GTCCTACAGTGTGCATTTCTCATTT6120.11124400609297236No Hit
CCACTGCTTCCCATGTACTCTGCGT5840.10615441104296708No Hit
CCATGTACTCTGCGTTGATACCACT5720.10397315602153626No Hit
GTACTCTGCGTTGATACCACTGCTT5680.10324607101439265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAC402.665095E-416.699053
TAACCCT508.3869934E-515.2649084
CCGGTTT456.867079E-414.74501412
TAGGCTG500.001450053413.3567945
ACTGTTC2600.012.7915328
GATCTAC603.937202E-412.7219221
TAGGACC2200.012.5762034
GTCCTAC5500.012.4906141
GATATAC2950.012.2906711
ATACACT2900.012.1724494
TTAGCAC550.00296374112.1447653
CTGCTAG550.00296577612.1436531
ATAGGAC802.7312804E-511.9278943
TAGACTG802.7364084E-511.925715
TAGGACA1209.34233E-911.925714
TGTAGGA5850.011.9085962
GACGTGG3050.011.8367477
GTCCTAG657.7215984E-411.7433131
CACTGTT2950.011.5938887
TATACAC3550.011.5582973
Produced by FastQC (version 0.11.5)