##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926566_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 550142 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.95069091252804 31.0 28.0 31.0 25.0 34.0 2 29.325566853648695 31.0 30.0 33.0 25.0 34.0 3 29.691754128933983 31.0 30.0 33.0 25.0 34.0 4 33.447160914818355 35.0 33.0 37.0 28.0 37.0 5 33.30400696547437 35.0 33.0 37.0 28.0 37.0 6 33.47594621025117 35.0 35.0 37.0 28.0 37.0 7 33.33378836736697 35.0 35.0 37.0 28.0 37.0 8 33.33839808631226 36.0 35.0 37.0 28.0 37.0 9 34.59248521290867 38.0 35.0 39.0 27.0 39.0 10 34.51430539751555 38.0 35.0 39.0 27.0 39.0 11 34.78620974221201 38.0 35.0 39.0 27.0 39.0 12 34.60262077790825 38.0 35.0 39.0 27.0 39.0 13 34.7678163092438 38.0 35.0 39.0 27.0 39.0 14 35.45882335833294 39.0 35.0 41.0 27.0 41.0 15 35.598758138807796 39.0 36.0 41.0 27.0 41.0 16 35.552046562523856 39.0 36.0 41.0 27.0 41.0 17 35.514107266851106 39.0 36.0 41.0 27.0 41.0 18 35.49560113570678 39.0 36.0 41.0 27.0 41.0 19 35.51362557303387 39.0 35.0 41.0 27.0 41.0 20 35.5313282752453 39.0 36.0 41.0 27.0 41.0 21 35.438037815691224 39.0 36.0 41.0 27.0 41.0 22 35.41846650501143 39.0 36.0 41.0 27.0 41.0 23 35.114663123339064 39.0 35.0 41.0 25.0 41.0 24 35.21140541896456 39.0 35.0 41.0 27.0 41.0 25 35.023682976395186 39.0 35.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5125941563965135 1101 2 0.6140468520716063 1101 3 0.6410060018147874 1101 4 0.33640342142712854 1101 5 0.4130884770182348 1101 6 -0.04771327566496808 1101 7 0.39247421628923007 1101 8 0.6183730450773766 1101 9 0.726870120746959 1101 10 0.8700355309994734 1101 11 0.5870148230068821 1101 12 0.7993420831067013 1101 13 0.7546965854021366 1101 14 0.9254105006877325 1101 15 0.8494669170358478 1101 16 0.819584957490747 1101 17 0.5378469197711411 1101 18 0.6919093615017147 1101 19 0.6859312797275194 1101 20 0.665280676267713 1101 21 0.6618707876249132 1101 22 0.6404049790996211 1101 23 0.7583559852592501 1101 24 0.6028569573996663 1101 25 0.768517063542042 1102 1 0.9190599053340414 1102 2 0.9627160328345674 1102 3 0.8698594563174851 1102 4 0.6888091008210608 1102 5 0.8275910051128363 1102 6 0.4048524044008843 1102 7 0.8155552580993799 1102 8 0.7324155187465564 1102 9 1.0559560171706721 1102 10 1.21486847517982 1102 11 1.118285295533795 1102 12 1.070870782439826 1102 13 1.0550516525100306 1102 14 1.3491435137397545 1102 15 1.1303541858537756 1102 16 1.1477417161543002 1102 17 1.2459548803295064 1102 18 1.1640845727361935 1102 19 1.233315205749257 1102 20 1.2463256360365733 1102 21 1.2029796216777626 1102 22 1.2709777059142198 1102 23 1.4143387925361353 1102 24 1.3696654269365425 1102 25 1.4322983287651994 1103 1 0.3273985899206231 1103 2 0.2192573746575519 1103 3 0.2247628624278626 1103 4 0.06191543549898881 1103 5 0.2497506201843791 1103 6 -0.02975113556619391 1103 7 0.17897733179291464 1103 8 0.0910146812377377 1103 9 0.5060887433251864 1103 10 0.6580924319115482 1103 11 0.4521390570879831 1103 12 0.562009559278998 1103 13 0.23262997676884112 1103 14 0.44414107175940387 1103 15 0.5386442569425398 1103 16 0.3443392235254663 1103 17 0.5328174782348327 1103 18 0.535400121820004 1103 19 0.4464039943461131 1103 20 0.7103256450809496 1103 21 0.7320798875822874 1103 22 0.6564917880009347 1103 23 0.9639686516248247 1103 24 0.690164043147675 1103 25 0.7754768375291974 1104 1 0.6777136862335844 1104 2 0.8577742266215758 1104 3 0.6317717923823629 1104 4 0.5779789910502728 1104 5 0.6609964444504826 1104 6 0.3083692687228279 1104 7 0.3929960463452531 1104 8 0.5001249654814259 1104 9 0.6619062470615802 1104 10 0.8737349687920783 1104 11 0.4849156971218278 1104 12 0.3421653821242572 1104 13 0.3399795889210182 1104 14 0.6420508278335078 1104 15 0.6349689701295773 1104 16 0.5790798339331005 1104 17 0.5265978113961651 1104 18 0.6887883018890761 1104 19 0.7051397845653611 1104 20 0.6412576443160418 1104 21 0.8179971816105791 1104 22 0.4839809634090031 1104 23 0.7340621175560642 1104 24 0.5828934855035826 1104 25 0.7104758707139283 1105 1 0.32353398310074155 1105 2 0.49150466693227557 1105 3 0.4901236636953499 1105 4 0.2789760998240496 1105 5 0.26752121448472366 1105 6 -0.023549888719756495 1105 7 -0.08916807191059206 1105 8 0.09925250777946104 1105 9 0.5546474428935042 1105 10 0.49079070847992057 1105 11 0.19499267340020054 1105 12 0.38654874854152155 1105 13 0.38562244014711666 1105 14 0.5323439125433467 1105 15 0.6064259704998349 1105 16 0.488220280461384 1105 17 0.5765879810274157 1105 18 0.537900624580665 1105 19 0.7733825038549398 1105 20 0.5681725951592043 1105 21 0.42954991081036553 1105 22 0.6302748145881978 1105 23 0.8447351036029289 1105 24 0.5851439912436192 1105 25 0.8464932440194417 1106 1 0.4468668472919788 1106 2 0.5425824306795342 1106 3 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0.41146185736014473 1107 15 0.26297558946567534 1107 16 0.28207762941644177 1107 17 0.3005538483579855 1107 18 0.4842427158406011 1107 19 0.33193420904764537 1107 20 -0.11015561865791312 1107 21 0.23420819030713602 1107 22 0.2808443007347705 1107 23 0.5315561433737912 1107 24 0.2632964318024875 1107 25 0.3731906085731751 1108 1 1.3686682771881742 1108 2 1.1050760739234242 1108 3 0.9594631200736892 1108 4 0.8110848241561044 1108 5 -0.36503868158464314 1108 6 0.6160041763577411 1108 7 0.8989292522784567 1108 8 0.9485279138843765 1108 9 1.3803974655528037 1108 10 1.3307647258218296 1108 11 0.7624750746812055 1108 12 0.9349308748897442 1108 13 0.8142174631588901 1108 14 1.402738788521475 1108 15 1.1707411059017119 1108 16 1.2359457907990574 1108 17 1.3091075939059493 1108 18 1.274410287511067 1108 19 1.3690218484474244 1108 20 1.3874037904621872 1108 21 1.4022177658311605 1108 22 1.3964570758851167 1108 23 1.732788116282066 1108 24 1.584076486686584 1108 25 1.5112766715147288 1109 1 0.513057562533092 1109 2 -0.24341911689258566 1109 3 0.6092373125099968 1109 4 0.8157457680661935 1109 5 -0.6556478448900549 1109 6 0.1564323488678241 1109 7 0.983801869232984 1109 8 1.1306736028560067 1109 9 1.5372389176160937 1109 10 -0.8056816491910936 1109 11 0.360665166727955 1109 12 0.9297700516401832 1109 13 1.2360053074723325 1109 14 1.601187438847255 1109 15 1.5312816650770813 1109 16 1.4413824095462573 1109 17 1.3591300022901294 1109 18 1.2043858106606464 1109 19 1.2541461287935505 1109 20 1.441177017614251 1109 21 1.2348098987645884 1109 22 1.2166522011996932 1109 23 1.342537487123586 1109 24 1.3192783345164187 1109 25 1.4047161861146549 1110 1 0.7709992795191773 1110 2 -0.2844344155870644 1110 3 0.41944758005815075 1110 4 0.8430972561685337 1110 5 0.9883412717953064 1110 6 0.8337197940733532 1110 7 1.0911592445084466 1110 8 1.1905636559201227 1110 9 1.2941503793057123 1110 10 1.3626994577565625 1110 11 1.1014028136089422 1110 12 1.2533457933046677 1110 13 1.2033394522808791 1110 14 1.5957457815284641 1110 15 1.1712850064456148 1110 16 1.079846334699596 1110 17 1.366994151792504 1110 18 1.4172230303238038 1110 19 1.5238781033036801 1110 20 1.5290510321094288 1110 21 1.6741680377814347 1110 22 1.5785860580140962 1110 23 1.9899753734693206 1110 24 1.7981402007502965 1110 25 1.8325664928045526 1111 1 0.12418123812282289 1111 2 0.14218815862587064 1111 3 0.35948745985706765 1111 4 0.15722088354517894 1111 5 0.3093315265205021 1111 6 0.009359415496106749 1111 7 0.25841568284922545 1111 8 0.3709144296443867 1111 9 0.7045121059325012 1111 10 0.6165259525469153 1111 11 0.2927415953091739 1111 12 0.49739892892406345 1111 13 0.28693256599447636 1111 14 0.6340458348887594 1111 15 0.4406632185949064 1111 16 0.5199423832293846 1111 17 0.39496617976453763 1111 18 0.5876389597758802 1111 19 0.2577646986360591 1111 20 0.06919763249699429 1111 21 0.4339203336783086 1111 22 0.42921233936326786 1111 23 0.715233047522176 1111 24 0.6401796873680965 1111 25 0.9624145563875572 1112 1 0.14686624042712282 1112 2 0.09560016731636978 1112 3 0.49670024542556845 1112 4 0.73824991771464 1112 5 0.923312038733286 1112 6 0.33476349081377776 1112 7 0.7505892953319417 1112 8 0.5991840655651188 1112 9 0.44811356093283194 1112 10 0.9805846463384782 1112 11 0.3512666792514949 1112 12 0.598343691581249 1112 13 0.43144092730858574 1112 14 0.8594762450540117 1112 15 0.6407216490379923 1112 16 0.7714566369656382 1112 17 0.73069022348038 1112 18 0.8086212495908782 1112 19 0.8497885316731271 1112 20 0.5221487813547157 1112 21 0.7806227169820161 1112 22 0.6056887367561217 1112 23 0.9084691065122357 1112 24 0.7117569801457648 1112 25 0.8229797680538908 1113 1 -0.03504408761743605 1113 2 -0.11856223846086777 1113 3 -1.188479326904126 1113 4 -1.2083571843534315 1113 5 -1.3708167191954814 1113 6 -0.42464670545069794 1113 7 -0.22321502002658633 1113 8 -0.3168753145413561 1113 9 0.159847918636693 1113 10 0.2296149645434511 1113 11 -0.04875945713210683 1113 12 0.10151959163923863 1113 13 -0.2016778241190309 1113 14 0.22360987884594152 1113 15 -0.32926214201150117 1113 16 -0.44323508548792745 1113 17 -0.34726318786675847 1113 18 -0.2623203630459514 1113 19 -3.1682609473562735 1113 20 -1.2333178958543556 1113 21 -0.845338179473984 1113 22 -0.8097919091420067 1113 23 -0.325511925940809 1113 24 -0.7233709783171491 1113 25 -0.27936284259745037 1114 1 0.7346646464103515 1114 2 -0.9059768516456295 1114 3 -0.3772798860241551 1114 4 0.25507774879419287 1114 5 0.36113783007573375 1114 6 0.01973270589272147 1114 7 -0.003896237643807865 1114 8 0.01822255615966384 1114 9 -0.03738636352134961 1114 10 -0.12764890355953895 1114 11 -0.047383883564847906 1114 12 0.12682446517043644 1114 13 -0.7772874477008571 1114 14 -0.23907799684369735 1114 15 -0.18923887827182284 1114 16 -0.25118822214140835 1114 17 -3.4176298263798586 1114 18 -1.1762507002466975 1114 19 -0.639219551729461 1114 20 -0.5085511506540428 1114 21 -0.18077809135824197 1114 22 -0.4768450699976796 1114 23 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20 0.40075550826064443 2202 21 0.34633317013270926 2202 22 0.483089689508418 2202 23 0.7102358093450647 2202 24 0.4318510262915254 2202 25 0.6552500140402984 2203 1 0.15019277325011515 2203 2 0.2590742050140662 2203 3 -0.07702511024713843 2203 4 -0.4234277019863768 2203 5 -0.062388835102197504 2203 6 -0.1813726758649281 2203 7 -0.08454647414880867 2203 8 0.27430773856288226 2203 9 0.580637753898813 2203 10 0.6588317395716672 2203 11 0.22944877645667106 2203 12 0.2356116918701261 2203 13 0.22875713298490297 2203 14 0.7167422593531398 2203 15 0.5449924297125079 2203 16 0.3015534088295837 2203 17 0.4327782208805431 2203 18 0.4186811903722756 2203 19 0.49321310017171527 2203 20 0.6125383964337985 2203 21 0.754051076915573 2203 22 0.7806002699402015 2203 23 0.8057979503051129 2203 24 0.7925822491571068 2203 25 0.7915018305945125 2204 1 0.23887400710009743 2204 2 0.1969518092409217 2204 3 0.183020837860159 2204 4 -0.14830763135387315 2204 5 0.09755472361106854 2204 6 -0.02476659536791459 2204 7 0.34789848288462366 2204 8 0.28434675285117095 2204 9 0.6174956244767742 2204 10 0.7001469001357989 2204 11 0.27622431949026094 2204 12 0.4104340705398499 2204 13 0.40454677940635975 2204 14 0.8345108639451766 2204 15 0.7128590322798658 2204 16 0.6488799593470773 2204 17 0.33288153478377325 2204 18 0.5292016428270756 2204 19 0.5376207440137932 2204 20 0.766650613360639 2204 21 0.7582333254144018 2204 22 0.5622323522585049 2204 23 0.827915677306791 2204 24 0.7828321439941845 2204 25 0.6292666446988306 2205 1 0.65903001770128 2205 2 0.6960554947716631 2205 3 0.734845602822471 2205 4 0.46173257308841187 2205 5 0.5412298564699682 2205 6 0.6260582998326498 2205 7 0.35991957936363406 2205 8 0.50935821487613 2205 9 0.8981939979810178 2205 10 1.0737210295248545 2205 11 0.7801584676420958 2205 12 1.0182168119597392 2205 13 0.9852272583866508 2205 14 1.4352617986428697 2205 15 1.0408794214487074 2205 16 1.0314151157335658 2205 17 1.2134996476421023 2205 18 1.130443882797941 2205 19 1.0220975091921076 2205 20 1.1010039698544958 2205 21 1.07654937659062 2205 22 0.8215536521421356 2205 23 1.3889460630555561 2205 24 1.026370261977334 2205 25 1.1576495832761395 2206 1 0.47084770695381195 2206 2 0.7637116911797293 2206 3 0.7447843122914328 2206 4 0.6010335727255409 2206 5 0.6859987866941992 2206 6 0.5993449734916396 2206 7 0.8270429010817608 2206 8 0.742193635136303 2206 9 1.0389285896011629 2206 10 1.1797720403463856 2206 11 1.0039588782339735 2206 12 0.8397674176573204 2206 13 0.6671905437181778 2206 14 0.9167856611890315 2206 15 1.0669743745487708 2206 16 1.0031896580429205 2206 17 1.0483323538203635 2206 18 1.011584990203005 2206 19 1.0044400700725404 2206 20 0.9247874795700142 2206 21 1.0372578215608712 2206 22 1.0592823731931702 2206 23 1.0513232210240702 2206 24 1.1543506569607587 2206 25 1.0914627689422076 2207 1 -1.033147475529379 2207 2 -0.07099121216390358 2207 3 0.03885422551689999 2207 4 0.2933847775983409 2207 5 0.4434753161077083 2207 6 0.5415643876894904 2207 7 0.5239105279611351 2207 8 0.4267469782757871 2207 9 0.7792861520166383 2207 10 0.5132873468214072 2207 11 0.33982371134847966 2207 12 0.45325843910909214 2207 13 0.2849245338659614 2207 14 -5.324874012838826 2207 15 -2.1113665437410063 2207 16 -0.8838129185388155 2207 17 -0.612715850963589 2207 18 -0.14679778520001463 2207 19 0.19024813941319252 2207 20 -0.07610099689029681 2207 21 0.3872889182369832 2207 22 0.3805910002996029 2207 23 0.6386199820337737 2207 24 0.32706751925677935 2207 25 0.25621098458531577 2208 1 0.6663657240982204 2208 2 1.0471781975216103 2208 3 0.929448406003857 2208 4 0.7565473231619109 2208 5 0.724444735260974 2208 6 0.7627114061358355 2208 7 0.961800086605983 2208 8 0.9921829683662011 2208 9 1.1821154326250962 2208 10 1.099477443550299 2208 11 0.8910482075850439 2208 12 0.9309401805447237 2208 13 0.8656331952832872 2208 14 1.0779237085882798 2208 15 1.054583466515723 2208 16 1.048999556805576 2208 17 0.9021840275335649 2208 18 0.9521284136543855 2208 19 0.6546000716240741 2208 20 0.9706933792635866 2208 21 1.0329542646778975 2208 22 0.9909830442299778 2208 23 1.0207464396734593 2208 24 1.1147274848572692 2208 25 1.118997581400997 2209 1 0.0271007696520833 2209 2 0.10599627065514028 2209 3 0.49125221521357076 2209 4 0.7810144872794851 2209 5 0.7250930017407526 2209 6 0.9482269274286566 2209 7 1.123589466991028 2209 8 0.9931363014247054 2209 9 1.0843003689321407 2209 10 0.8486292347858182 2209 11 0.8982358235309604 2209 12 0.8105615269601927 2209 13 0.9330920731329755 2209 14 1.6131465308663877 2209 15 1.3804296140195405 2209 16 1.3711776909524218 2209 17 1.3207951500961244 2209 18 1.3586770387935232 2209 19 1.3094100233905763 2209 20 1.2102077462504326 2209 21 1.4041110441642317 2209 22 1.2860123141314546 2209 23 1.468035881896327 2209 24 1.3003491610744433 2209 25 1.2197118082221152 2210 1 -0.265099434053937 2210 2 -0.7246477858345663 2210 3 0.16782661853957848 2210 4 0.43121789548371225 2210 5 0.6401428263272351 2210 6 0.7650785377187859 2210 7 0.9303320171283218 2210 8 0.7848260488173224 2210 9 1.1276826036229224 2210 10 0.9975037888066325 2210 11 0.7861486707097498 2210 12 0.6066756261566795 2210 13 0.7684285156634587 2210 14 1.080140794933719 2210 15 0.7815660791840244 2210 16 1.0909842297965398 2210 17 1.2510526416530467 2210 18 1.3761719312522303 2210 19 1.1312792345955955 2210 20 1.1112566535641974 2210 21 0.8935129704369444 2210 22 1.127914999834509 2210 23 1.2515249729028852 2210 24 1.066718607553966 2210 25 1.0418037856595888 2211 1 -1.149658154153439 2211 2 -0.4468992103729015 2211 3 0.34614994493838935 2211 4 0.5672122590254034 2211 5 0.7904664784758282 2211 6 0.8128292601676392 2211 7 0.7414279923910811 2211 8 0.7845015329382576 2211 9 0.8942014489880208 2211 10 0.9041493310936417 2211 11 0.7400797548889244 2211 12 0.9788867021427521 2211 13 0.8304776792021897 2211 14 1.022412751579381 2211 15 0.6871393617793586 2211 16 0.9516867141321725 2211 17 0.9531729765830477 2211 18 0.9399288133332746 2211 19 0.888042861177766 2211 20 0.904660960864696 2211 21 0.840549017611437 2211 22 0.9827823678503194 2211 23 0.9983285531890971 2211 24 0.8831080167376371 2211 25 1.1807150904108497 2212 1 0.7473856838125599 2212 2 0.10637980894808763 2212 3 0.4042011985327001 2212 4 0.37974820212181015 2212 5 0.46568718169935863 2212 6 0.4505707725522399 2212 7 0.6949945564832944 2212 8 0.54647247578243 2212 9 0.7888541251722501 2212 10 0.5120896024955499 2212 11 0.49485703380734947 2212 12 0.6608293949743143 2212 13 0.33259895247061166 2212 14 0.4132743769640399 2212 15 0.4092071618096256 2212 16 -0.2981392456115657 2212 17 0.43256307898933954 2212 18 0.3920753098272485 2212 19 0.463513274334197 2212 20 0.3454141263795023 2212 21 0.4834486796202171 2212 22 0.448559495429393 2212 23 0.8748482525282455 2212 24 0.5972077998179017 2212 25 -0.07386544734831801 2213 1 0.19257891642728353 2213 2 0.06373566251001961 2213 3 -0.3883212934041538 2213 4 -1.540548295360228 2213 5 -2.061919902553459 2213 6 -0.6721842775241456 2213 7 -0.5163476038743156 2213 8 -0.5618311512192165 2213 9 -1.0270345185215177 2213 10 -0.8314117837999291 2213 11 -0.9164455181079987 2213 12 -0.9532796979923575 2213 13 -0.851328507496568 2213 14 -8.46063214711224 2213 15 -3.9382465811881637 2213 16 -2.286069987421101 2213 17 -1.409170073276826 2213 18 -5.3330364425634045 2213 19 -3.3028280372710768 2213 20 -5.367908456090937 2213 21 -2.439778842588808 2213 22 -2.110208116181539 2213 23 -4.642423857470053 2213 24 -2.512767869281859 2213 25 -1.6691775052898095 2214 1 0.031322063002726 2214 2 -0.12805083549776342 2214 3 -2.1160605085834554 2214 4 -2.380647210082742 2214 5 -0.7379310934416381 2214 6 -0.917634664365572 2214 7 -0.833226088596227 2214 8 -0.7604694163663623 2214 9 -5.774792031598551 2214 10 -1.9880941627918247 2214 11 -1.0906666972616605 2214 12 -1.3148090309809461 2214 13 -1.5297893625918064 2214 14 -2.9459772547449816 2214 15 -2.4813162524226797 2214 16 -1.4893755424241846 2214 17 -1.853898488718663 2214 18 -1.4099207613976432 2214 19 -4.8240065279597 2214 20 -2.0792326006973667 2214 21 -3.5871995682728226 2214 22 -5.711582447413264 2214 23 -5.945204342364345 2214 24 -5.755380756040413 2214 25 -3.570769952766227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13057.0 3 10.0 4 209.0 5 1309.0 6 1814.0 7 1504.0 8 1189.0 9 970.0 10 901.0 11 801.0 12 795.0 13 763.0 14 781.0 15 825.0 16 824.0 17 899.0 18 1021.0 19 1242.0 20 1583.0 21 2108.0 22 2636.0 23 3361.0 24 3935.0 25 4825.0 26 5582.0 27 6230.0 28 7400.0 29 9041.0 30 11334.0 31 14376.0 32 19082.0 33 25797.0 34 34097.0 35 46573.0 36 65386.0 37 99951.0 38 157931.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.13091491258392 19.36398202557898 9.727654774682993 28.77744828715411 2 12.842827243980807 21.465263142582312 39.09617073583187 26.59573887760501 3 19.855659342957253 24.958677986504203 28.439546212466976 26.74611645807157 4 12.687393518661622 17.7332225412243 35.65717963776799 33.92220430234609 5 12.9792796986138 38.77601856026997 34.791415147856696 13.45328659325954 6 33.15998073048962 35.815742606055295 17.280882310005545 13.743394353449535 7 27.89502065919972 31.722802363966274 20.926275441421875 19.455901535412128 8 26.072176780281637 37.94673273459275 19.467509518517844 16.513580966607762 9 26.670905493592567 15.861896832539616 19.472976567424677 37.994221106443135 10 16.108383071326372 30.0844106615956 32.4991046252243 21.308101641853725 11 33.66852622375398 23.427147352176117 22.624429454075486 20.279896969994418 12 24.264667121108836 25.910656668938874 30.360917973187913 19.463758236764374 13 29.908278226348834 21.52789643401158 24.70398551646666 23.859839823172926 14 21.414733793790916 23.44313615634942 26.411068444709617 28.731061605150042 15 25.632389081972455 29.40454723922812 22.34629255311414 22.616771125685283 16 21.347708012956844 28.038077995542903 29.426467006220303 21.18774698527995 17 22.681596742528903 29.208899876390603 27.587071911582928 20.522431469497565 18 22.651174458215024 26.909653424851758 29.88733490145894 20.551837215474272 19 24.041327962540173 27.444668227492837 27.39995201186616 21.11405179810083 20 23.801621044863207 27.499050240030247 27.793884818200493 20.905443896906053 21 21.87530105918301 27.206116250197677 27.79723743549381 23.121345255125505 22 22.91299337262016 28.55281000178136 25.48069407534782 23.053502550250663 23 21.173624264280857 28.34431837598293 27.521621690399932 22.96043566933628 24 22.95029819628059 28.643747693773054 27.38952377663181 21.016430333314553 25 22.831559851820078 26.913233310672517 26.541874643273918 23.713332194233487 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 245.0 1 245.0 2 243.0 3 241.0 4 241.0 5 241.0 6 343.5 7 446.0 8 446.0 9 446.0 10 712.5 11 979.0 12 979.0 13 979.0 14 1403.5 15 1828.0 16 1828.0 17 1828.0 18 3369.0 19 4910.0 20 4910.0 21 4910.0 22 7933.5 23 10957.0 24 10957.0 25 10957.0 26 16454.0 27 21951.0 28 21951.0 29 21951.0 30 29778.0 31 37605.0 32 37605.0 33 37605.0 34 42675.5 35 47746.0 36 47746.0 37 47746.0 38 51073.5 39 54401.0 40 54401.0 41 54401.0 42 59176.0 43 63951.0 44 63951.0 45 63951.0 46 78854.0 47 93757.0 48 93757.0 49 93757.0 50 86756.0 51 79755.0 52 79755.0 53 79755.0 54 65919.0 55 52083.0 56 52083.0 57 52083.0 58 43383.5 59 34684.0 60 34684.0 61 34684.0 62 28616.5 63 22549.0 64 22549.0 65 22549.0 66 17598.5 67 12648.0 68 12648.0 69 12648.0 70 9216.5 71 5785.0 72 5785.0 73 5785.0 74 4053.0 75 2321.0 76 2321.0 77 2321.0 78 1594.5 79 868.0 80 868.0 81 868.0 82 595.0 83 322.0 84 322.0 85 322.0 86 206.5 87 91.0 88 91.0 89 91.0 90 54.0 91 17.0 92 17.0 93 17.0 94 9.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.085796030842946 2 0.03508185159467919 3 0.03508185159467919 4 0.029628714041102115 5 0.027265687767885384 6 0.3864456812968288 7 0.22430572470380372 8 0.22030675716451387 9 0.2268505222288064 10 0.016904726416088936 11 0.0019994837696449283 12 0.0030901112803603434 13 0.0 14 0.07252672946257512 15 0.0010906275107154153 16 0.0014541700142872204 17 0.003998967539289857 18 0.0029083400285744407 19 0.0010906275107154153 20 5.453137553577077E-4 21 1.8177125178590254E-4 22 0.0 23 0.0 24 1.8177125178590254E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 550142.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.86562856000995 #Duplication Level Percentage of deduplicated Percentage of total 1 91.25567863689704 66.49402383547776 2 6.111710367708962 8.906672350396862 3 1.2205529481386719 2.6680907327069265 4 0.4455016122036806 1.2984701999087596 5 0.24000581762555329 0.8744087379672527 6 0.15448824487812274 0.6754129840906291 7 0.10749403739250529 0.5482834420740679 8 0.06412345160688562 0.3737916485418481 9 0.05207208588905082 0.3414838741862861 >10 0.29249604205677787 3.8534025817087167 >50 0.025673107929776482 1.2803202115962777 >100 0.02013576511536536 2.8927784163054393 >500 0.0062924265985499815 3.0305123027340635 >1k 0.0032720618312459906 4.227201529031556 >5k 5.033941278839986E-4 2.5351471532735994 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 6926 1.2589476898691612 No Hit GTACATGGGAAGCAGTGGTATCAAC 6897 1.2536763235673698 No Hit GAGTACATGGGAAGCAGTGGTATCA 3273 0.594937307095259 No Hit CATGTACTCTGCGTTGATACCACTG 2596 0.47187816963620305 No Hit GCGTTGATACCACTGCTTCCCATGT 2118 0.3849915112825416 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1987 0.3611794772985884 No Hit GTATCAACGCAGAGTACATGGGAAG 1941 0.35281799971643685 No Hit GCTTCCCATGTACTCTGCGTTGATA 1674 0.3042850754896009 No Hit ACTCTGCGTTGATACCACTGCTTCC 1619 0.29428765664137624 No Hit GTATCAACGCAGAGTACTTTTTTTT 1549 0.28156366901636304 No Hit TATCAACGCAGAGTACATGGGAAGC 1395 0.25357089624133405 No Hit CTGTAGGACGTGGAATATGGCAAGA 1372 0.24939015745025828 No Hit GTCCTACAGTGGACATTTCTAAATT 1251 0.22739583598416407 No Hit GGTATCAACGCAGAGTACATGGGAA 1228 0.22321509719308835 No Hit GGTATCAACGCAGAGTACTTTTTTT 1046 0.19013272936805406 No Hit CAGTGGTATCAACGCAGAGTACATG 953 0.17322800295196514 No Hit CTTTAGGACGTGAAATATGGCGAGG 850 0.15450556401801718 No Hit TATCAACGCAGAGTACTTTTTTTTT 813 0.14778002770193877 No Hit GTCCTAAAGTGTGTATTTCTCATTT 812 0.14759825645015287 No Hit CATGGGAAGCAGTGGTATCAACGCA 795 0.14450814516979255 No Hit GTGGTATCAACGCAGAGTACATGGG 756 0.13741906635014234 No Hit GCAGTGGTATCAACGCAGAGTACAT 745 0.1354195825804974 No Hit ACATGGGAAGCAGTGGTATCAACGC 728 0.13232947130013706 No Hit ATACCACTGCTTCCCATGTACTCTG 704 0.1279669612572754 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 692 0.12578570623584456 No Hit GGGAAGCAGTGGTATCAACGCAGAG 678 0.12324090871084192 No Hit GATATACACTGTTCTACAAATCCCG 642 0.11669714364654943 No Hit AAGCAGTGGTATCAACGCAGAGTAC 633 0.11506120238047632 No Hit CTGCTTCCCATGTACTCTGCGTTGA 630 0.1145158886251186 No Hit GTCCTACAGTGTGCATTTCTCATTT 612 0.11124400609297236 No Hit CCACTGCTTCCCATGTACTCTGCGT 584 0.10615441104296708 No Hit CCATGTACTCTGCGTTGATACCACT 572 0.10397315602153626 No Hit GTACTCTGCGTTGATACCACTGCTT 568 0.10324607101439265 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 1.8177125178590254E-4 3 0.0 0.0 0.0 0.0 1.8177125178590254E-4 4 0.0 0.0 0.0 0.0 1.8177125178590254E-4 5 0.0 0.0 0.0 0.0 1.8177125178590254E-4 6 0.0 0.0 0.0 0.0 1.8177125178590254E-4 7 0.0 0.0 0.0 1.8177125178590254E-4 1.8177125178590254E-4 8 0.0 0.0 0.0 1.8177125178590254E-4 1.8177125178590254E-4 9 0.0 0.0 0.0 1.8177125178590254E-4 1.8177125178590254E-4 10 0.0 0.0 0.0 1.8177125178590254E-4 1.8177125178590254E-4 11 0.0 0.0 0.0 1.8177125178590254E-4 1.8177125178590254E-4 12 0.0 0.0 0.0 3.635425035718051E-4 1.8177125178590254E-4 13 0.0 0.0 0.0 3.635425035718051E-4 1.8177125178590254E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAACAC 40 2.665095E-4 16.69905 3 TAACCCT 50 8.3869934E-5 15.264908 4 CCGGTTT 45 6.867079E-4 14.745014 12 TAGGCTG 50 0.0014500534 13.356794 5 ACTGTTC 260 0.0 12.791532 8 GATCTAC 60 3.937202E-4 12.721922 1 TAGGACC 220 0.0 12.576203 4 GTCCTAC 550 0.0 12.490614 1 GATATAC 295 0.0 12.290671 1 ATACACT 290 0.0 12.172449 4 TTAGCAC 55 0.002963741 12.144765 3 CTGCTAG 55 0.002965776 12.143653 1 ATAGGAC 80 2.7312804E-5 11.927894 3 TAGACTG 80 2.7364084E-5 11.92571 5 TAGGACA 120 9.34233E-9 11.92571 4 TGTAGGA 585 0.0 11.908596 2 GACGTGG 305 0.0 11.836747 7 GTCCTAG 65 7.7215984E-4 11.743313 1 CACTGTT 295 0.0 11.593888 7 TATACAC 355 0.0 11.558297 3 >>END_MODULE