FastQCFastQC Report
Thu 26 May 2016
SRR1926565_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926565_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847481
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG704.7436097E-617.0251261
TAGGACC2050.016.1368124
TAACGCA453.4910598E-416.1186225
GCCTTAC550.00481630114.4455611
GTCTATA550.00481630114.4455611
GTTCTAG852.9843024E-514.0206921
GATATAC3150.013.8723241
TTCACTA600.007375858713.5456972
GCCTATA600.007375858713.5456972
GTATAGA905.108139E-513.2417641
AAAGGCG550.001596364813.18796355
TCCAACG1550.012.71845318
GTATTAA1255.1511597E-712.7120931
CTTGGAT600.002140909212.6831423
TCTCAAC600.002140909212.6831423
TCTAGGA1502.0336302E-812.6426512
CCAACGT753.085932E-612.56435819
GAGTATA958.477393E-512.5448291
GTCATAC750.002599032412.3589791
GGGTGGG750.002599032412.3589791
Produced by FastQC (version 0.11.5)