##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926565_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 847481 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.03500963443428 26.0 2.0 30.0 2.0 31.0 2 15.767934620363171 16.0 2.0 30.0 2.0 31.0 3 19.195586685719206 20.0 10.0 30.0 2.0 31.0 4 21.231316100301953 27.0 2.0 35.0 2.0 35.0 5 21.6957076323835 28.0 2.0 35.0 2.0 35.0 6 22.180081913340832 29.0 2.0 35.0 2.0 35.0 7 21.977374124021658 29.0 2.0 35.0 2.0 35.0 8 22.376817887362666 30.0 2.0 35.0 2.0 37.0 9 24.930772489294746 31.0 17.0 37.0 2.0 39.0 10 25.538702342589392 31.0 16.0 37.0 2.0 39.0 11 22.749592026251914 29.0 2.0 35.0 2.0 39.0 12 23.727090046856507 30.0 2.0 35.0 2.0 39.0 13 20.638841460752513 26.0 2.0 35.0 2.0 39.0 14 23.764014768472684 30.0 7.0 37.0 2.0 40.0 15 24.796395435413892 31.0 2.0 37.0 2.0 40.0 16 25.145377890477782 31.0 2.0 38.0 2.0 40.0 17 24.411733124400428 30.0 2.0 37.0 2.0 40.0 18 24.44749793800687 30.0 2.0 37.0 2.0 40.0 19 24.82056470882533 30.0 2.0 38.0 2.0 40.0 20 25.026835999863124 31.0 2.0 38.0 2.0 40.0 21 22.909410358462313 30.0 2.0 36.0 2.0 40.0 22 23.804823942955654 30.0 2.0 37.0 2.0 40.0 23 23.765538106459022 30.0 2.0 37.0 2.0 40.0 24 22.925213662607185 30.0 2.0 37.0 2.0 40.0 25 22.69707521466558 30.0 2.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.899328986697647 1101 2 9.551980063185773 1101 3 6.5004386240109895 1101 4 7.696822146819631 1101 5 7.513136895707163 1101 6 6.689117375545564 1101 7 6.353705500445745 1101 8 5.170761448000235 1101 9 4.949969378972753 1101 10 4.6956250864341555 1101 11 7.2714029387590315 1101 12 5.21294842347854 1101 13 9.472750860507116 1101 14 6.88285430444629 1101 15 6.015901880995489 1101 16 5.46123870956426 1101 17 1.3576371390373758 1101 18 3.9559976274418496 1101 19 4.6563147620250085 1101 20 2.666215356071657 1101 21 -0.40710927817074705 1101 22 3.312306020862394 1101 23 4.501603802528976 1101 24 5.849512108602099 1101 25 6.266703495124936 1102 1 4.75324042328652 1102 2 9.47574842176352 1102 3 6.22581325394383 1102 4 7.146299095167294 1102 5 7.311621177833906 1102 6 6.110209492654384 1102 7 6.015554055012114 1102 8 6.416438659935743 1102 9 5.165745515322431 1102 10 4.5899021414804935 1102 11 7.200358818505411 1102 12 4.782869399771428 1102 13 8.853097420615498 1102 14 6.410837260736688 1102 15 4.177585473792629 1102 16 4.793356766033831 1102 17 5.6968064336854205 1102 18 5.6146601384758625 1102 19 4.6408671452838455 1102 20 4.932939232883825 1102 21 3.666867300767848 1102 22 4.757837048591767 1102 23 4.985470892480524 1102 24 6.5228597269226825 1102 25 6.200654733127916 1103 1 4.178341637861418 1103 2 8.264547342141388 1103 3 5.718390851784587 1103 4 7.441170890039089 1103 5 6.864927520613936 1103 6 6.298468601966125 1103 7 6.125540559735462 1103 8 5.89484621729067 1103 9 5.095570076725938 1103 10 4.260617391143114 1103 11 4.9041374959683 1103 12 3.839549561714751 1103 13 7.773475288361787 1103 14 6.231350081249509 1103 15 5.290945797679267 1103 16 5.076082811917772 1103 17 2.583446109798782 1103 18 1.3413807862491431 1103 19 3.389854999534858 1103 20 3.78786500886223 1103 21 6.110861902657188 1103 22 1.6714672780114697 1103 23 3.915295453884031 1103 24 5.308959591969916 1103 25 1.1709651244909374 1104 1 5.001233286801238 1104 2 9.473987173793532 1104 3 6.336348719073406 1104 4 7.194779161042543 1104 5 7.0818812478263595 1104 6 6.719898643824354 1104 7 6.497953011987342 1104 8 6.677377839697403 1104 9 5.646131617474811 1104 10 4.644466518524801 1104 11 7.699169404291911 1104 12 4.553403949966995 1104 13 8.77330373823484 1104 14 6.702209433018762 1104 15 5.982544416128913 1104 16 5.49575160580601 1104 17 5.918712241229052 1104 18 3.5645903747575716 1104 19 4.370046678210034 1104 20 3.4736881063713234 1104 21 2.4480608321843924 1104 22 4.4434885959739105 1104 23 4.969013516968733 1104 24 5.963960850004181 1104 25 5.263030359285878 1105 1 2.670791149416914 1105 2 8.294934162722875 1105 3 5.498062633979799 1105 4 4.7373195863319815 1105 5 1.501276031511832 1105 6 3.7956229736698823 1105 7 4.767172299781045 1105 8 2.0213400070865504 1105 9 2.820250587275229 1105 10 2.45110678639098 1105 11 5.382617679567446 1105 12 2.696036141705296 1105 13 3.0372062701893796 1105 14 2.296985719343688 1105 15 2.6519338843342624 1105 16 3.1226401958940464 1105 17 3.655002767512613 1105 18 1.1728773358423048 1105 19 1.395930514983121 1105 20 0.5806504498091734 1105 21 1.5386106739531833 1105 22 2.6592026860785296 1105 23 0.13549690588072494 1105 24 3.2439709876878737 1105 25 3.9286126680072613 1106 1 6.007180938637397 1106 2 10.391940645110349 1106 3 7.300661959240728 1106 4 8.754395733999953 1106 5 8.486266399662519 1106 6 7.323954115630091 1106 7 7.342076490909552 1106 8 7.142489815468807 1106 9 6.182688413433091 1106 10 5.567540046338795 1106 11 7.638979031899474 1106 12 5.089660265156045 1106 13 4.34744100192454 1106 14 5.723964580237361 1106 15 5.613177157328391 1106 16 5.901975292518312 1106 17 5.957470865196893 1106 18 6.0756529901965095 1106 19 6.046895738401254 1106 20 5.422941657816349 1106 21 5.744528400632895 1106 22 6.2967572155958145 1106 23 6.615212689882682 1106 24 7.361414572570457 1106 25 6.015194839403218 1107 1 3.1095354057199174 1107 2 -13.037746854889651 1107 3 -5.458315496068195 1107 4 1.0357187283021752 1107 5 3.7232358618843797 1107 6 4.0622391630215375 1107 7 3.8392242280017967 1107 8 4.522714182232686 1107 9 2.6660153333942063 1107 10 2.1563163074103002 1107 11 4.801868137942897 1107 12 2.0175022329136603 1107 13 3.412925751600497 1107 14 3.0093199823220473 1107 15 2.712572612610309 1107 16 2.293430233515288 1107 17 2.9967417997939982 1107 18 -1.7617448177803752 1107 19 0.5663400703303978 1107 20 1.1096184677374978 1107 21 -2.1302386926917976 1107 22 0.7602693469956847 1107 23 -2.1456794945794044 1107 24 1.0797742851163612 1107 25 1.805454783505617 1108 1 5.015631838718303 1108 2 10.06192952051488 1108 3 0.4438038989656441 1108 4 5.7003719172523795 1108 5 6.790454557024976 1108 6 7.042915230112293 1108 7 7.032681264372332 1108 8 6.7756082418442105 1108 9 5.241492013756716 1108 10 4.642929407180219 1108 11 6.755487678744135 1108 12 2.264597057227242 1108 13 3.510398627329071 1108 14 4.7735929176160035 1108 15 5.050560854739395 1108 16 4.861950767841936 1108 17 0.015756160504334815 1108 18 3.6153715884974815 1108 19 4.5606750668802185 1108 20 4.680998140421526 1108 21 4.495347575390177 1108 22 1.7253095011945199 1108 23 4.221175725885917 1108 24 4.889002305175634 1108 25 5.523409341830401 1109 1 3.764203779156677 1109 2 -13.037746854889651 1109 3 -5.0430487657119585 1109 4 2.2737777388201543 1109 5 1.7891384683578089 1109 6 2.6408884995016315 1109 7 3.8983405375147093 1109 8 3.980252450020501 1109 9 3.4861228083940574 1109 10 2.7193006774010122 1109 11 0.9566397731166489 1109 12 1.2736975921610139 1109 13 2.549260222116164 1109 14 3.2196018155697494 1109 15 3.3194903670516 1109 16 3.2924344132008265 1109 17 3.929835093668622 1109 18 4.147265263232942 1109 19 2.2607423642588387 1109 20 1.4180877240276075 1109 21 0.647218695168025 1109 22 2.894313660769697 1109 23 3.4405389962169615 1109 24 4.486986140023731 1109 25 4.8912843754216695 1110 1 3.2002059582094198 1110 2 8.796630319779798 1110 3 6.233370890608157 1110 4 7.757968987740384 1110 5 8.295815645052219 1110 6 3.8147375946662763 1110 7 6.3832538569499135 1110 8 7.304043290590588 1110 9 5.477492040781733 1110 10 5.401052789901772 1110 11 2.906853455612069 1110 12 5.855108573263212 1110 13 10.210026357700464 1110 14 7.9463489965560825 1110 15 7.2670839509957545 1110 16 6.946663390552409 1110 17 7.371139206359036 1110 18 7.307721481410844 1110 19 6.2148291500226875 1110 20 6.350102463748847 1110 21 3.9413468592271883 1110 22 6.357824652095466 1110 23 6.93647796693557 1110 24 7.784968145552362 1110 25 8.173785327399042 1111 1 5.066698118156605 1111 2 9.186103911266244 1111 3 7.229716634596045 1111 4 7.808088671347274 1111 5 8.133688517556145 1111 6 7.178138565580124 1111 7 7.2111672359684 1111 8 7.4913032801034944 1111 9 5.848333191914104 1111 10 5.228160396660801 1111 11 6.835721568125322 1111 12 6.2233706040967505 1111 13 7.761022052057786 1111 14 5.618357033879377 1111 15 5.317483661325724 1111 16 5.3496269448889535 1111 17 6.411184882241084 1111 18 6.217907546186961 1111 19 5.212443359431678 1111 20 4.7482503929651365 1111 21 7.463267990906047 1111 22 6.755120910632456 1111 23 6.698679180971958 1111 24 6.441264551057021 1111 25 6.938009317251318 1112 1 -1.9540779395999337 1112 2 7.20672735810432 1112 3 5.500584950561333 1112 4 -0.5203626000310813 1112 5 3.577675178826393 1112 6 2.7108573201679356 1112 7 5.364155219420379 1112 8 5.577615017801918 1112 9 4.238160826360083 1112 10 3.135933870295034 1112 11 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2.650474053801606 2204 4 5.1729219627923655 2204 5 0.44774838361128744 2204 6 3.141487018574203 2204 7 4.2278985966979725 2204 8 4.439263455688369 2204 9 2.754352377172278 2204 10 2.650431554988163 2204 11 4.0880665339786475 2204 12 4.24822074835631 2204 13 2.492159377673044 2204 14 3.4131565088800784 2204 15 3.6647770824780928 2204 16 3.498164424670673 2204 17 -1.5279047377638548 2204 18 2.1431806952031636 2204 19 2.9615819908962635 2204 20 3.3480536733685753 2204 21 4.7722126155364215 2204 22 4.238818405715573 2204 23 3.1664786475506794 2204 24 4.554061830138654 2204 25 4.539456212490379 2205 1 5.750964554241708 2205 2 9.779677872848978 2205 3 6.875425815876429 2205 4 7.673132304440909 2205 5 7.703809824683873 2205 6 7.239241541558982 2205 7 7.139044913928984 2205 8 6.993240702987492 2205 9 5.879342201069221 2205 10 5.227652087231384 2205 11 7.4060191404036395 2205 12 6.665070158653801 2205 13 9.771260368516469 2205 14 7.073519319453677 2205 15 5.983377549705072 2205 16 5.708726857353888 2205 17 6.389691630464881 2205 18 6.101678391248186 2205 19 4.5857046616342245 2205 20 4.933471349653846 2205 21 -1.0374923392774207 2205 22 3.3534040966015795 2205 23 4.725634945247833 2205 24 6.646280126835883 2205 25 7.066927002279581 2206 1 3.760244367254966 2206 2 8.447395263973398 2206 3 5.729950759305325 2206 4 1.5617025411704937 2206 5 5.273062870915748 2206 6 5.626103057813552 2206 7 5.6381439973695 2206 8 5.666320968569583 2206 9 4.363223781650145 2206 10 3.3879332956928003 2206 11 3.0466068177521883 2206 12 4.2845589247167695 2206 13 7.289533621433584 2206 14 -0.22291243849648978 2206 15 2.041693791695316 2206 16 2.989108892324513 2206 17 3.7790046991762196 2206 18 -1.3695535262763592 2206 19 0.47307064150203004 2206 20 1.7672114817827556 2206 21 2.856729628023075 2206 22 -2.482645642931317 2206 23 -3.2610035972620217 2206 24 0.7562233984748481 2206 25 2.2405965674393933 2207 1 1.758272924859174 2207 2 -13.037746854889651 2207 3 -5.59565122552023 2207 4 -4.250104494253701 2207 5 1.2187349629095294 2207 6 1.9333057715065856 2207 7 2.974691862755499 2207 8 3.417239773968145 2207 9 1.1686300634994922 2207 10 1.119384750854067 2207 11 -1.2395199928349214 2207 12 -2.2770447896248314 2207 13 0.41125346042387534 2207 14 -5.368057954623197 2207 15 -1.2233828526317687 2207 16 -0.2077239314192738 2207 17 0.9602358469365981 2207 18 0.8867082483306419 2207 19 0.1901249050679219 2207 20 0.2248512071191371 2207 21 -3.4126553643073443 2207 22 -4.10477727145598 2207 23 -0.952544925489164 2207 24 0.6519788778493414 2207 25 1.2918849539450505 2208 1 4.70998227792311 2208 2 6.371142033999238 2208 3 6.162885421542313 2208 4 7.899989732015825 2208 5 3.781423889741884 2208 6 6.176088292217393 2208 7 6.571451490909549 2208 8 6.578295867056109 2208 9 5.026512818194995 2208 10 4.593189847926094 2208 11 6.672645946582012 2208 12 6.287460364362396 2208 13 8.704690505892795 2208 14 3.429527576269109 2208 15 4.8364261652649 2208 16 5.1491937945024375 2208 17 6.1218433850381615 2208 18 0.9800441112282563 2208 19 3.511077286020303 2208 20 4.3956537113877765 2208 21 7.051661337140832 2208 22 5.97855037035772 2208 23 1.981089425993794 2208 24 3.4284197809037877 2208 25 4.6205891846413145 2209 1 2.842265396621322 2209 2 4.5192063247050385 2209 3 4.753864756672591 2209 4 0.38387113363307535 2209 5 4.57176031781416 2209 6 5.156513569246442 2209 7 5.735867982873213 2209 8 4.249203324378662 2209 9 4.0935189078425935 2209 10 3.2200721480159444 2209 11 -0.5497479837364452 2209 12 3.033823546951993 2209 13 2.2253973043354414 2209 14 3.565648171257628 2209 15 3.9552401814374036 2209 16 3.6243899610193573 2209 17 1.1315269230235856 2209 18 3.344396711807274 2209 19 3.952878224425014 2209 20 4.010548390433399 2209 21 5.983161786377128 2209 22 4.795717186769817 2209 23 0.22517048818806273 2209 24 -3.07145443293269 2209 25 -0.25424359962543264 2210 1 -2.3477553092881607 2210 2 -13.037746854889651 2210 3 -7.398963344516979 2210 4 -2.3589531206112397 2210 5 -1.2379466085759852 2210 6 -1.7901078287155698 2210 7 -1.7102365128837178 2210 8 -19.616624767864526 2210 9 -12.521492329810153 2210 10 -7.179729838677801 2210 11 -9.406126209032248 2210 12 -4.631617962084352 2210 13 -5.3063474493556875 2210 14 -4.162383733536938 2210 15 -5.745564396725662 2210 16 -5.2975722196319985 2210 17 -4.806129700829661 2210 18 -5.093659517212213 2210 19 -9.95212260560064 2210 20 -6.932728053836094 2210 21 -5.067549302069807 2210 22 -9.343651079212151 2210 23 -7.62911130104904 2210 24 -10.054738113833054 2210 25 -7.767394287552685 2211 1 0.6125702253076319 2211 2 5.113573630477944 2211 3 0.8893026065462237 2211 4 1.8250609396566269 2211 5 1.3880790000372514 2211 6 0.6430663011225946 2211 7 -19.215215175757116 2211 8 -12.483862455230692 2211 9 -8.670825817473403 2211 10 -6.604585890492039 2211 11 -8.789349226933503 2211 12 -4.142431209683355 2211 13 -5.717900160637916 2211 14 -4.165497745779767 2211 15 -4.44204567457739 2211 16 -10.777133658677263 2211 17 -7.325728077522594 2211 18 -5.151312402826331 2211 19 -8.166406144182613 2211 20 -6.00422392703376 2211 21 -3.657494707861037 2211 22 -5.17055129172039 2211 23 -7.1979160462879115 2211 24 -20.29158021909621 2211 25 -20.074648910596782 2212 1 -0.5968651647576664 2212 2 -13.037746854889651 2212 3 -7.249323573166681 2212 4 -18.519692807666715 2212 5 -18.85032214200415 2212 6 -19.36549900936991 2212 7 -9.080184311559584 2212 8 -8.653661804901562 2212 9 -4.538279950764441 2212 10 -5.003736959834047 2212 11 -4.909752642483241 2212 12 -2.3679496885476574 2212 13 -5.331716019680398 2212 14 -3.18011528087375 2212 15 -3.193053552548154 2212 16 -3.368592801617492 2212 17 -3.465127514432737 2212 18 -2.51030862245781 2212 19 -2.978856576413559 2212 20 -3.080602902368838 2212 21 -1.0137663524535263 2212 22 -2.385100423293075 2212 23 -2.300947611043245 2212 24 -1.39266046600979 2212 25 -1.8462538488683862 2213 1 -17.43097010302927 2213 2 -13.037746854889651 2213 3 -8.212151279838293 2213 4 -5.328540887800271 2213 5 -18.85032214200415 2213 6 -9.93812004442834 2213 7 -19.215215175757116 2213 8 -9.959696554175043 2213 9 -7.807464445498461 2213 10 -6.951758611145902 2213 11 -6.17394811229919 2213 12 -4.911428789518414 2213 13 -17.78927774807546 2213 14 -11.475480240992466 2213 15 -6.74827161145291 2213 16 -5.765692391468889 2213 17 -4.402310628264434 2213 18 -4.518619799522465 2213 19 -5.017721505616553 2213 20 -5.0438247845180975 2213 21 -3.212092008382129 2213 22 -4.064241584487657 2213 23 -3.7792370440488696 2213 24 -2.9585251272765127 2213 25 -3.7925120157720755 2214 1 -17.43097010302927 2214 2 -13.037746854889651 2214 3 -12.08609221328898 2214 4 -18.519692807666715 2214 5 -18.85032214200415 2214 6 -19.36549900936991 2214 7 -15.5669975397346 2214 8 -14.50874484291143 2214 9 -13.900200306079103 2214 10 -13.7502611184517 2214 11 -13.631164172837861 2214 12 -12.194616528934011 2214 13 -17.78927774807546 2214 14 -16.007285908349253 2214 15 -22.19151034267161 2214 16 -17.42408176462678 2214 17 -21.814664551469775 2214 18 -16.867088322733444 2214 19 -14.450588040523073 2214 20 -13.350428661523866 2214 21 -10.926760295427142 2214 22 -11.943209658231577 2214 23 -11.892610803316192 2214 24 -10.81597046299865 2214 25 -10.576525083204661 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 176645.0 3 12.0 4 976.0 5 3511.0 6 4501.0 7 4300.0 8 3564.0 9 3663.0 10 3450.0 11 3329.0 12 3161.0 13 3223.0 14 4587.0 15 8610.0 16 12248.0 17 13157.0 18 14041.0 19 16303.0 20 19480.0 21 22302.0 22 23604.0 23 25954.0 24 27079.0 25 27011.0 26 22837.0 27 19518.0 28 21035.0 29 23819.0 30 23385.0 31 26821.0 32 33318.0 33 39854.0 34 46296.0 35 50806.0 36 47460.0 37 44659.0 38 22962.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.881686177230364 17.589485496374937 13.178239093417094 25.3505892329776 2 14.007360663372936 19.39032520250957 42.199979908859284 24.40233422525822 3 19.971251101167674 22.907282305701614 28.338378532159087 28.78308806097162 4 11.80699646652725 16.007706426512065 38.131300418655975 34.05399668830471 5 13.736769629549627 36.72872340425532 35.69000432012096 13.844502646074089 6 32.94677591891495 35.93912853617642 17.177430539820907 13.936665005087729 7 28.25163809341213 30.357517611609282 20.796207965966 20.594636329012584 8 25.25503156624328 37.391644207253805 19.609518123059335 17.74380610344358 9 25.702601079934272 15.964173871544288 19.176216402668295 39.15700864585314 10 15.009464424897004 29.53187912833904 33.23707276891598 22.221583677847978 11 34.40084062767532 21.862684168095583 23.60696163513065 20.12951356909845 12 24.157129059217407 23.914414256617107 30.718435815696893 21.210020868468597 13 28.82562723241307 20.501396323501787 24.52659276193763 26.146383682147512 14 20.68253616194798 21.867772855609974 27.03181292298839 30.417878059453656 15 23.450897176452592 30.22770230324455 23.038515279495865 23.28288524080699 16 22.565556842672084 27.907711718851612 26.993021613428258 22.533709825048042 17 21.54695462119199 27.555432581044855 28.562812265251164 22.33480053251199 18 21.578552029585722 27.414591836612452 29.789739639427022 21.217116494374807 19 23.347146772480563 26.352018793620992 26.39859574676985 23.902238687128595 20 23.581521887051903 27.6185945567267 26.956143060175634 21.843740496045765 21 22.598924341756778 25.53936894387902 26.166754128571462 25.694952585792734 22 22.556749840204635 27.255393168553233 27.61012089283937 22.577736098402763 23 22.866029408985238 26.80439528246612 27.394913908786783 22.934661399761854 24 23.136179303352886 26.737738328005545 27.408884474928357 22.71719789371321 25 23.239319318846317 26.648631726924236 27.582471456163876 22.529577498065574 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 838.0 1 838.0 2 1705.5 3 2573.0 4 2573.0 5 2573.0 6 3696.0 7 4819.0 8 4819.0 9 4819.0 10 5681.5 11 6544.0 12 6544.0 13 6544.0 14 7683.5 15 8823.0 16 8823.0 17 8823.0 18 11960.0 19 15097.0 20 15097.0 21 15097.0 22 21499.0 23 27901.0 24 27901.0 25 27901.0 26 37104.5 27 46308.0 28 46308.0 29 46308.0 30 57302.5 31 68297.0 32 68297.0 33 68297.0 34 76000.0 35 83703.0 36 83703.0 37 83703.0 38 89036.5 39 94370.0 40 94370.0 41 94370.0 42 97929.0 43 101488.0 44 101488.0 45 101488.0 46 102378.5 47 103269.0 48 103269.0 49 103269.0 50 98052.5 51 92836.0 52 92836.0 53 92836.0 54 83117.0 55 73398.0 56 73398.0 57 73398.0 58 62821.5 59 52245.0 60 52245.0 61 52245.0 62 42765.0 63 33285.0 64 33285.0 65 33285.0 66 25686.0 67 18087.0 68 18087.0 69 18087.0 70 13302.5 71 8518.0 72 8518.0 73 8518.0 74 5942.0 75 3366.0 76 3366.0 77 3366.0 78 2276.5 79 1187.0 80 1187.0 81 1187.0 82 788.0 83 389.0 84 389.0 85 389.0 86 246.5 87 104.0 88 104.0 89 104.0 90 66.0 91 28.0 92 28.0 93 28.0 94 18.0 95 8.0 96 8.0 97 8.0 98 4.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.618807973276096 2 35.3961917730309 3 5.434812107882064 4 13.275813853053933 5 12.597450562313492 6 14.07205589269848 7 8.91430014360204 8 7.314618262828311 9 0.1118609148759677 10 1.4452241407181992 11 5.448499730377436 12 5.686145176116042 13 15.198924813653639 14 1.57950443726762 15 2.8000627742686857 16 2.926083298622624 17 2.7684396464345515 18 1.504930494016975 19 1.4518319584745853 20 0.2876760658941026 21 4.212365822950604 22 1.6049917343279672 23 0.11056295067382042 24 8.752644602061874 25 0.11516482375416087 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 847481.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.41840605798795 #Duplication Level Percentage of deduplicated Percentage of total 1 96.47897462433181 89.16433052889813 2 2.6227564222858613 4.847819360520209 3 0.43294472136854895 1.2003618318030305 4 0.16763300996414848 0.6196950233435767 5 0.08411841271431325 0.38870448115924094 6 0.04987965051452557 0.2765878677169171 7 0.033635060253471585 0.217594905940316 8 0.021347573815796307 0.15783269962089075 9 0.014420451884798296 0.119944366004607 >10 0.0816972082027565 1.3719906374812303 >50 0.007346583016951572 0.49161268860159113 >100 0.005115124638925052 1.0569793724458134 >500 1.3115700811884828E-4 0.0865462364644648 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 1.1799674564975498E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCTAG 70 4.7436097E-6 17.025126 1 TAGGACC 205 0.0 16.136812 4 TAACGCA 45 3.4910598E-4 16.118622 5 GCCTTAC 55 0.004816301 14.445561 1 GTCTATA 55 0.004816301 14.445561 1 GTTCTAG 85 2.9843024E-5 14.020692 1 GATATAC 315 0.0 13.872324 1 TTCACTA 60 0.0073758587 13.545697 2 GCCTATA 60 0.0073758587 13.545697 2 GTATAGA 90 5.108139E-5 13.241764 1 AAAGGCG 55 0.0015963648 13.1879635 5 TCCAACG 155 0.0 12.718453 18 GTATTAA 125 5.1511597E-7 12.712093 1 CTTGGAT 60 0.0021409092 12.683142 3 TCTCAAC 60 0.0021409092 12.683142 3 TCTAGGA 150 2.0336302E-8 12.642651 2 CCAACGT 75 3.085932E-6 12.564358 19 GAGTATA 95 8.477393E-5 12.544829 1 GTCATAC 75 0.0025990324 12.358979 1 GGGTGGG 75 0.0025990324 12.358979 1 >>END_MODULE