FastQCFastQC Report
Thu 26 May 2016
SRR1926565_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926565_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847481
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15370.1813609980636734No Hit
GATATACACTGTTCTACAAATCCCG14930.1761691412550842No Hit
GTCCTACAGTGGACATTTCTAAATT14870.17546116078118565No Hit
CTGTAGGACGTGGAATATGGCAAGA14600.17227524864864227No Hit
CCCATGTACTCTGCGTTGATACCAC10630.12543054062568953No Hit
GTGTATATCAATGAGTTACAATGAA10430.12307060571269444No Hit
GTCCTAAAGTGTGTATTTCTCATTT9900.11681677819325743No Hit
GTACATGGGAAGCAGTGGTATCAAC9730.11481083351721158No Hit
GGTATCAACGCAGAGTACTTTTTTT9660.11398485629766332No Hit
GATATACACTGTTCTACAATGCCGG9330.11009096369122139No Hit
CTTTAGGACGTGAAATATGGCGAGG9230.10891099623472386No Hit
GTCCTACAGTGTGCATTTCTCATTT8990.10607907433912973No Hit
GTGTATATCAATGAGTTACAATGAG8530.100651224039241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3200.013.702874
TTGGACA655.2752974E-513.1987514
GTGTTAG603.9846476E-412.7053851
AATCCCG2250.012.63641919
GATATAC4700.012.570221
TGTAGGA9200.012.540942
CCAACGA1457.2759576E-1212.4185519
CTATTAC701.0591479E-412.251621
GCTACTC550.002985173612.1343463
GTAGGAC8200.012.0920653
GACGTGG4050.011.9597987
TACACTT802.7645878E-511.9155395
TCCAACG2150.011.90174518
AGGACGT6750.011.7213595
GGACGTG6700.011.6665586
ACTGTTC4600.011.5655778
TGTAGAA4950.011.5578912
TAGGACG6850.011.5502454
AGGACCT5300.011.510865
CTGTAGG9000.011.2230891