FastQCFastQC Report
Thu 26 May 2016
SRR1926564_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926564_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524202
Sequences flagged as poor quality0
Sequence length25
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC26100.4978996646330994No Hit
CCCATGTACTCTGCGTTGATACCAC25160.4799676460601066No Hit
GAGTACATGGGAAGCAGTGGTATCA12770.24360837997565826No Hit
CATGTACTCTGCGTTGATACCACTG9860.18809542886139313No Hit
GCGTTGATACCACTGCTTCCCATGT8600.16405889332738144No Hit
ACGCAGAGTACATGGGAAGCAGTGG8150.1554744163509487No Hit
GTATCAACGCAGAGTACATGGGAAG7150.1363978008477648No Hit
GCTTCCCATGTACTCTGCGTTGATA6330.120754976135154No Hit
ACTCTGCGTTGATACCACTGCTTCC5940.1133150960889123No Hit
CNCATGTACTCTGCGTTGATACCAC5550.10587521604267058No Hit
GNACATGGGAAGCAGTGGTATCAAC5490.10473061911247955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGA450.001231463517.7178361
CCTAGGA707.146888E-515.549172
TCTAGAA550.00401760114.8423882
TAGGACC651.7539975E-514.8310494
CTAACAC450.004569106314.5592183
GTATAGC658.5969135E-414.3105611
GTAGGAC1301.8189894E-1114.2792343
CTGTAGG1501.2187229E-1014.1742711
TGAAGTG450.00630251513.8703515
TAGCCCT851.0191361E-613.8617014
TGTAGGA1202.2452514E-713.6055232
GTATTAA700.001504518313.2883791
GTACTGT550.001557052413.2295336
ATACTGT653.7508615E-412.7933966
ATAGCCC600.002068319112.7393173
CCTACAC704.624721E-412.4793313
TGTACTG852.0949012E-512.2385455
TAGGACG1006.301276E-611.7824464
GAGTGGT856.7138553E-611.71679313
AGGACCT801.6187989E-411.7031095