FastQCFastQC Report
Thu 26 May 2016
SRR1926564_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926564_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524202
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC75341.437232212009874No Hit
GTACATGGGAAGCAGTGGTATCAAC68971.3157141712545928No Hit
GAGTACATGGGAAGCAGTGGTATCA32120.6127408899622665No Hit
CATGTACTCTGCGTTGATACCACTG28190.5377697910347538No Hit
GCGTTGATACCACTGCTTCCCATGT23650.4511619566502989No Hit
ACGCAGAGTACATGGGAAGCAGTGG20420.389544488575015No Hit
GTATCAACGCAGAGTACATGGGAAG19550.372947833087245No Hit
ACTCTGCGTTGATACCACTGCTTCC16830.32105943891858485No Hit
GCTTCCCATGTACTCTGCGTTGATA15400.2937798787490319No Hit
GTATCAACGCAGAGTACTTTTTTTT13040.24875906616151788No Hit
TATCAACGCAGAGTACATGGGAAGC12840.24494374306088113No Hit
GGTATCAACGCAGAGTACATGGGAA11570.22071644137183757No Hit
CAGTGGTATCAACGCAGAGTACATG10810.20621821358941783No Hit
GGTATCAACGCAGAGTACTTTTTTT8430.16081586869184017No Hit
CATGGGAAGCAGTGGTATCAACGCA8320.15871744098648993No Hit
ACATGGGAAGCAGTGGTATCAACGC8000.1526129240254711No Hit
GCAGTGGTATCAACGCAGAGTACAT7750.14784377014967512No Hit
GTGGTATCAACGCAGAGTACATGGG7660.14612687475438857No Hit
ATACCACTGCTTCCCATGTACTCTG7600.14498227782419754No Hit
CCATGTACTCTGCGTTGATACCACT6950.13258247774712803No Hit
TATCAACGCAGAGTACTTTTTTTTT6800.12972098542165042No Hit
GGGAAGCAGTGGTATCAACGCAGAG6650.12685949309617287No Hit
AAGCAGTGGTATCAACGCAGAGTAC6320.12056420998012216No Hit
CTGCTTCCCATGTACTCTGCGTTGA6110.11655812072445355No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5990.11426892686407149No Hit
CCACTGCTTCCCATGTACTCTGCGT5880.11217049915872125No Hit
GATACCACTGCTTCCCATGTACTCT5700.10873670836814815No Hit
GCAGAGTACATGGGAAGCAGTGGTA5520.10530291757757505No Hit
GTACTCTGCGTTGATACCACTGCTT5470.10434908680241586No Hit
CTTCCCATGTACTCTGCGTTGATAC5430.10358602218228852No Hit
TACCACTGCTTCCCATGTACTCTGC5370.10244142525209748No Hit
ATGGGAAGCAGTGGTATCAACGCAG5250.10015223139171542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTAG551.08059685E-515.612281
TCTAGAC602.4468776E-514.3195123
TAAACTC400.005115113714.3153835
TATGCTG500.001446436913.3610245
TAAGCTG500.001446436913.3610245
GTCCTAG500.001449584513.3571731
GTCCAGA655.2243406E-513.210391
TCCTAGG603.9196262E-412.7284562
TTTAATC550.002954065612.1498893
ATAAGAC550.002954065612.1498893
TAGACTG550.002960456812.1463855
CTACCCC550.002960456812.1463854
TTAGACA657.7059603E-411.7459554
ACTGTTC1302.6084308E-911.6886588
ATGAGTT1302.6957423E-911.66411311
GTAGAAC1251.6932972E-811.4556093
TAAGACA751.9826356E-411.4523074
CTTCTAA751.9879555E-411.4490051
GATATAC1104.67664E-711.2755361
TATAAAG600.005658027311.1373982
Produced by FastQC (version 0.11.5)