FastQCFastQC Report
Thu 26 May 2016
SRR1926563_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926563_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243786
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC11380.4668028516813927No Hit
CCCATGTACTCTGCGTTGATACCAC9600.393787994388521No Hit
GAGTACATGGGAAGCAGTGGTATCA6370.26129474210988324No Hit
CATGTACTCTGCGTTGATACCACTG4340.17802498912981057No Hit
GCGTTGATACCACTGCTTCCCATGT4320.1772045974748345No Hit
GCTTCCCATGTACTCTGCGTTGATA3730.15300304365303996No Hit
GTATCAACGCAGAGTACATGGGAAG3670.1505418686881117No Hit
ACGCAGAGTACATGGGAAGCAGTGG3650.14972147703313562No Hit
GTCCTACAGTGGACATTTCTAAATT2980.12223835659143675No Hit
ACTCTGCGTTGATACCACTGCTTCC2800.11485483169665198No Hit
GTATCAACGCAGAGTACTTTTTTTT2670.10952228593930743No Hit
GNACATGGGAAGCAGTGGTATCAAC2570.10542032766442701No Hit
TATCAACGCAGAGTACATGGGAAGC2500.1025489568720107No Hit
GGTATCAACGCAGAGTACATGGGAA2490.10213876104452266No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAG200.00953360726.4259341
ATAAGAC300.00791417718.1153813
TAGGACC803.068817E-815.9953414
CCAACGA406.01568E-414.99778319
GTCCTAC1900.014.6038051
TTAGGAC601.29717E-414.4923053
CCACACT450.005333027314.2180814
TCCTACA2200.014.1452012
CTGTAGG2550.013.9901991
TGTAGGA2850.013.7675642
TCCAACG502.0590733E-413.72807318
TCTGGAC500.00943689613.0430743
TGTCCAC500.001935372512.8478410
GGACGTG2100.012.820256
AGGACCT1555.456968E-1212.7000725
GTAGGAC3100.012.622333
GACGTGG1309.0221874E-1012.4871387
GGACCTG1503.8198777E-1112.4257796
TAGGACG2150.012.3994884
AGGACGT2200.012.24432855
Produced by FastQC (version 0.11.5)