##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926563_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 243786 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.02378315407776 27.0 7.0 30.0 2.0 31.0 2 16.455493752717548 16.0 2.0 30.0 2.0 31.0 3 19.97700442191102 24.0 15.0 31.0 2.0 31.0 4 22.21648494991509 28.0 2.0 35.0 2.0 37.0 5 22.542926993346622 28.0 2.0 35.0 2.0 37.0 6 23.324526428917164 30.0 2.0 35.0 2.0 37.0 7 23.339982607696914 30.0 7.0 35.0 2.0 37.0 8 23.444812253369758 30.0 8.0 35.0 2.0 37.0 9 26.10992837980852 31.0 17.0 37.0 2.0 39.0 10 26.820912603676994 32.0 23.0 37.0 2.0 39.0 11 23.896807035678833 30.0 7.0 37.0 2.0 39.0 12 25.00681745465285 31.0 10.0 37.0 2.0 39.0 13 21.742778502457075 27.0 2.0 35.0 2.0 39.0 14 25.0642735842091 31.0 17.0 38.0 2.0 40.0 15 26.114592306367058 31.0 17.0 38.0 2.0 40.0 16 26.468669242696464 31.0 17.0 38.0 2.0 40.0 17 25.776533517101065 31.0 10.0 38.0 2.0 40.0 18 25.866140795615827 31.0 11.0 38.0 2.0 40.0 19 26.26952737236757 31.0 16.0 38.0 2.0 40.0 20 26.493568129424986 31.0 16.0 39.0 2.0 40.0 21 24.368577358831107 30.0 2.0 37.0 2.0 40.0 22 25.29335564798635 31.0 2.0 38.0 2.0 40.0 23 25.2855414174727 31.0 2.0 38.0 2.0 40.0 24 24.342296112163947 31.0 2.0 38.0 2.0 40.0 25 24.13179181741363 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.131037410516008 1101 2 9.65854685907902 1101 3 6.471475026750067 1101 4 7.3267091355371505 1101 5 7.18667926196915 1101 6 6.959246663948303 1101 7 6.837239595933045 1101 8 5.217872211367226 1101 9 4.9839673022025615 1101 10 4.698641209654543 1101 11 7.326821464568461 1101 12 5.251221636861363 1101 13 9.664160608911597 1101 14 6.944515981735606 1101 15 5.764026997482656 1101 16 5.234381855888103 1101 17 0.7441852003449831 1101 18 2.876242753406757 1101 19 3.7155395660557815 1101 20 2.274049429024796 1101 21 -0.6905873088535515 1101 22 2.968000787005291 1101 23 4.021849524799936 1101 24 5.735622293301837 1101 25 5.5066262159169135 1102 1 4.774243395376267 1102 2 9.64999468024358 1102 3 6.025207794747935 1102 4 7.05590863827873 1102 5 6.835235214640804 1102 6 5.954357326145566 1102 7 6.040488424933866 1102 8 6.011637611948839 1102 9 4.983750861596086 1102 10 4.393615347776947 1102 11 7.029021389091735 1102 12 4.607632718620412 1102 13 8.723099050983212 1102 14 6.310650241508728 1102 15 3.9318350646125424 1102 16 4.690655303620471 1102 17 5.291224958819463 1102 18 5.177028599301046 1102 19 3.9770053684399613 1102 20 4.477836584662242 1102 21 3.677839001444582 1102 22 4.904439395569678 1102 23 5.183913591219454 1102 24 6.646465412710455 1102 25 6.94058408214552 1103 1 4.423730627338429 1103 2 9.025457990610363 1103 3 6.141973376756713 1103 4 8.094184327843731 1103 5 7.320909544404088 1103 6 6.731553360436216 1103 7 6.996118640728486 1103 8 6.422141966497808 1103 9 5.717344558301182 1103 10 4.981752328471401 1103 11 5.669742675218576 1103 12 4.862817246896558 1103 13 8.803800326650038 1103 14 6.779742039494039 1103 15 6.131695218784042 1103 16 6.0908815582535745 1103 17 3.6050367240603833 1103 18 2.9438953705678443 1103 19 4.436751372032745 1103 20 4.540573924635407 1103 21 7.024440634116203 1103 22 2.355919714827472 1103 23 4.654580075972849 1103 24 6.245386439610879 1103 25 2.225312462889999 1104 1 5.459134009498332 1104 2 10.215293935533552 1104 3 7.009026701522053 1104 4 7.264517106454509 1104 5 7.089227746443928 1104 6 6.842497871703674 1104 7 7.271870674253499 1104 8 7.069563605071348 1104 9 5.645999548332654 1104 10 4.3203363030310555 1104 11 7.805671670731364 1104 12 4.628186082473498 1104 13 9.156570918665327 1104 14 6.605438326148295 1104 15 5.7576035083162616 1104 16 5.333147023204447 1104 17 5.273345589185912 1104 18 3.372921091020629 1104 19 4.018655333630566 1104 20 2.988289950624985 1104 21 2.4129606233512924 1104 22 4.391575742904124 1104 23 4.771928611999606 1104 24 5.663548385643516 1104 25 5.037470223788102 1105 1 2.4629213781121955 1105 2 8.779692670563211 1105 3 5.55194033551032 1105 4 4.989445318848848 1105 5 1.377213221912232 1105 6 3.6677994335218287 1105 7 4.779625890639931 1105 8 2.241239690774705 1105 9 2.422830334305921 1105 10 2.3665928420334126 1105 11 5.114382618543257 1105 12 3.1611814438897454 1105 13 3.74071999983785 1105 14 2.289756034116852 1105 15 2.923030166434863 1105 16 3.051098503941663 1105 17 3.6128295804151627 1105 18 1.2589660114751133 1105 19 1.0255243165362273 1105 20 0.8432816809083086 1105 21 1.1408573138483895 1105 22 2.279425454945585 1105 23 -0.3609417608649501 1105 24 2.97643998501211 1105 25 3.339743183700179 1106 1 6.4213917572011425 1106 2 11.059820537651028 1106 3 7.875951839199004 1106 4 9.298968481167197 1106 5 9.02607889079519 1106 6 7.576666950823572 1106 7 7.553096731833573 1106 8 7.791633567071997 1106 9 6.578608545797646 1106 10 5.744550991657476 1106 11 8.090162895914805 1106 12 5.639699012023005 1106 13 5.259364565214792 1106 14 5.824014301713529 1106 15 5.874275402679093 1106 16 5.669829510079502 1106 17 6.075260468580389 1106 18 6.374535677835524 1106 19 6.232732062498247 1106 20 5.5665896981529706 1106 21 5.618347378597413 1106 22 6.465990712283535 1106 23 6.681316354509004 1106 24 7.7900106408202205 1106 25 6.439008709058854 1107 1 3.744203341971726 1107 2 -13.721500646859223 1107 3 -5.570338955758116 1107 4 2.0825570727505003 1107 5 4.433409123939221 1107 6 4.600390747050859 1107 7 4.605467536868058 1107 8 4.9214636165140675 1107 9 3.5605952562728582 1107 10 2.6406544106134184 1107 11 5.6138656976321535 1107 12 3.0662549676406137 1107 13 4.376120283594435 1107 14 3.8148816898915214 1107 15 3.782356189553326 1107 16 3.404133489588858 1107 17 4.2885331977617085 1107 18 -0.8065839593793456 1107 19 2.017973971738982 1107 20 2.218049341456247 1107 21 -1.4634892211317592 1107 22 2.1047171853108395 1107 23 -1.0114867274850567 1107 24 2.650675327004784 1107 25 3.4248426634797724 1108 1 4.387060484828869 1108 2 9.582070781712204 1108 3 0.11622498433404971 1108 4 4.731246588879532 1108 5 6.468475368178854 1108 6 6.6469057240093825 1108 7 6.523382283411834 1108 8 6.074617418357384 1108 9 4.365822790835072 1108 10 3.510542083424479 1108 11 5.741241849705887 1108 12 1.2909813501290905 1108 13 2.466486067004574 1108 14 3.391278579292443 1108 15 3.8948273877100057 1108 16 4.016172660095773 1108 17 -0.0783302815009641 1108 18 3.028309473800377 1108 19 3.7670379118311494 1108 20 3.604714088000023 1108 21 2.865067806518013 1108 22 -0.27100286077211067 1108 23 2.3907310144504272 1108 24 3.5767265942858906 1108 25 3.6829071796088044 1109 1 3.9889060077270244 1109 2 -13.721500646859223 1109 3 -5.455385148953715 1109 4 2.4954404542247133 1109 5 1.4253170462376374 1109 6 3.0628959837496446 1109 7 4.598698414649018 1109 8 4.647744127174882 1109 9 4.011061598080456 1109 10 3.291482360252914 1109 11 1.3327597068487442 1109 12 2.219905222309542 1109 13 2.9742505065124334 1109 14 3.985146060495449 1109 15 3.900231523048351 1109 16 3.871478538427965 1109 17 4.253618197651463 1109 18 4.5330407622487705 1109 19 3.1038778810724317 1109 20 2.2615194536869687 1109 21 0.9136302637975753 1109 22 3.1934323750447042 1109 23 3.656815259153092 1109 24 4.87044028191405 1109 25 5.317903761973806 1110 1 3.7818687644352487 1110 2 9.606290327012513 1110 3 6.35033488423225 1110 4 7.951412223423134 1110 5 8.621358596886012 1110 6 4.766847614067068 1110 7 7.26716833702568 1110 8 7.8235729239562986 1110 9 6.017221458972159 1110 10 5.765149523193735 1110 11 3.4669639823831844 1110 12 6.362860591730719 1110 13 11.113091725301146 1110 14 8.566843772709476 1110 15 7.53599849391971 1110 16 6.828206083916605 1110 17 7.689553371366358 1110 18 8.099520663145476 1110 19 6.608651205010652 1110 20 6.903503110735006 1110 21 4.808225042690392 1110 22 7.115269195556358 1110 23 7.202372090120601 1110 24 8.2035557849883 1110 25 8.38073547957487 1111 1 4.898149852082621 1111 2 8.920051864556303 1111 3 6.9975544853125236 1111 4 7.6432960833348496 1111 5 7.936481320559807 1111 6 7.1156149542768325 1111 7 7.04429715621027 1111 8 7.235627284632663 1111 9 5.462253400750271 1111 10 4.924874682902626 1111 11 6.207188930593038 1111 12 5.817838411446768 1111 13 7.823598346848019 1111 14 5.564213592338756 1111 15 5.2382512298496025 1111 16 5.139093403735693 1111 17 6.053141913541438 1111 18 6.006222471191116 1111 19 4.629898723964221 1111 20 4.138614120615809 1111 21 6.840942317281222 1111 22 6.085263447120912 1111 23 6.05452341497228 1111 24 6.060571587762734 1111 25 6.555379456190668 1112 1 -2.478842480130698 1112 2 7.108688032386059 1112 3 5.014936237703324 1112 4 -0.23350240033879643 1112 5 3.3924561633271004 1112 6 2.602347098672457 1112 7 4.822405194463048 1112 8 5.322385275500242 1112 9 3.4501809444738853 1112 10 2.477396867791054 1112 11 -0.5752591610757847 1112 12 2.6688873474336745 1112 13 6.936288964578697 1112 14 4.619083498429909 1112 15 2.375032913316474 1112 16 2.3327157867804083 1112 17 3.411772481302272 1112 18 3.6131730182747717 1112 19 3.412885283798804 1112 20 3.196779451881426 1112 21 -2.0527640667973515 1112 22 1.0877404006834155 1112 23 2.4760578338574355 1112 24 4.147523427169986 1112 25 4.4143761823042205 1113 1 -18.398653800885416 1113 2 -13.721500646859223 1113 3 -7.178690085352361 1113 4 -5.6677407804584465 1113 5 -0.7423804680580801 1113 6 -0.12158295194880608 1113 7 -20.592689145159593 1113 8 -9.345907407426587 1113 9 -4.672472069792107 1113 10 -3.9542989444500876 1113 11 -5.064801268761013 1113 12 -2.524382325829098 1113 13 -2.184787887699258 1113 14 -1.9735188945751538 1113 15 -2.2983564101994 1113 16 -2.3336095698693846 1113 17 -2.3392514487483496 1113 18 -3.0847676167919573 1113 19 -2.548216879109617 1113 20 -3.5947414869128664 1113 21 -1.0440894234471436 1113 22 -1.9925403172181042 1113 23 -2.3272785674311045 1113 24 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3.4354368824864423 2204 7 4.769497642995304 2204 8 4.935141539737145 2204 9 3.0888591152737312 2204 10 3.138628440404627 2204 11 4.857816654456943 2204 12 4.787762793344189 2204 13 3.1295240185177526 2204 14 4.190756898524462 2204 15 3.9631136714718025 2204 16 3.517209920102278 2204 17 -1.063691695428723 2204 18 2.231027705170373 2204 19 3.3625726091952863 2204 20 3.5301432289046772 2204 21 4.764133255590579 2204 22 4.311093217978296 2204 23 3.337352800978252 2204 24 4.687504946059402 2204 25 5.110990485824228 2205 1 6.179948819201922 2205 2 10.485922933926805 2205 3 7.345433889511842 2205 4 7.891775674968116 2205 5 8.568941291447725 2205 6 8.194297333491605 2205 7 7.944428758770538 2205 8 7.551642917421638 2205 9 6.411157709737129 2205 10 5.740940165146249 2205 11 8.077301191564903 2205 12 7.18014424782092 2205 13 10.221038078857351 2205 14 7.671310160702298 2205 15 6.149369652214062 2205 16 5.769798673820038 2205 17 6.782838230663728 2205 18 6.551615384592644 2205 19 5.269367528175501 2205 20 5.454390476022482 2205 21 -0.4699388216791185 2205 22 4.032393115522339 2205 23 5.293043085567085 2205 24 6.9284206834936235 2205 25 7.6301935177248374 2206 1 4.505551806591221 2206 2 9.056536736318346 2206 3 6.522871346149806 2206 4 2.3199273298675145 2206 5 6.213739887537997 2206 6 6.66697414857547 2206 7 6.715722069793678 2206 8 6.740609574565664 2206 9 5.483750861596086 2206 10 4.3235218898330245 2206 11 4.110797090026313 2206 12 5.444081316751255 2206 13 8.886650452852372 2206 14 1.719528746181627 2206 15 3.375760298257404 2206 16 3.910281471844769 2206 17 4.95243991209049 2206 18 -0.5215695315400772 2206 19 1.6031735927390258 2206 20 3.0035375192416787 2206 21 3.811016571538037 2206 22 -1.0161213520938723 2206 23 -1.9399181844814812 2206 24 2.239923356635689 2206 25 3.4803037083137447 2207 1 0.8387562710570293 2207 2 -13.721500646859223 2207 3 -6.421978381544676 2207 4 -4.69877546490601 2207 5 0.4784477474183184 2207 6 1.301614100165601 2207 7 2.7190614543607907 2207 8 2.4974452275386128 2207 9 0.19923641707693562 2207 10 -0.10533128930590863 2207 11 -2.7571683507052143 2207 12 -3.522048583067207 2207 13 0.06981233456195568 2207 14 -5.826920076068983 2207 15 -2.4943791042392895 2207 16 -1.3833434413088206 2207 17 0.04376311521023979 2207 18 0.22090004420120124 2207 19 -0.41691314161181126 2207 20 -0.572433479660134 2207 21 -3.7990990742630792 2207 22 -4.533260911474404 2207 23 -1.692249030085371 2207 24 -0.1545330408631358 2207 25 0.39907710081469716 2208 1 4.421126418894804 2208 2 5.8081696828111085 2208 3 5.554652182136248 2208 4 7.507654555912499 2208 5 2.4742102582887426 2208 6 5.117441438295099 2208 7 6.143574591104144 2208 8 6.060846813961781 2208 9 4.320184054571335 2208 10 3.9034335669409614 2208 11 6.096495970585007 2208 12 5.098158547931288 2208 13 8.344748429641935 2208 14 1.7859557770946388 2208 15 3.7737422228748407 2208 16 4.402573758996869 2208 17 5.044506256960574 2208 18 0.45176414413004906 2208 19 2.647360714028949 2208 20 3.9561563956923322 2208 21 6.145940059265264 2208 22 5.152005380986132 2208 23 1.1309095858789995 2208 24 2.328271923956219 2208 25 3.6026873993890227 2209 1 2.5115116837481537 2209 2 4.023180204204607 2209 3 4.736924283557286 2209 4 -0.024795499941813404 2209 5 4.111422218904959 2209 6 4.824770043496045 2209 7 5.211093360750574 2209 8 4.123803715216553 2209 9 3.5847152680387175 2209 10 2.5641926659979326 2209 11 -0.7558927754207581 2209 12 2.829772088055467 2209 13 1.2833606448498962 2209 14 2.8365831638922394 2209 15 3.5143288230359104 2209 16 2.4443008262558514 2209 17 0.4653152352163623 2209 18 2.5917012755565416 2209 19 3.0680605815373276 2209 20 3.492705898199283 2209 21 5.634766599155633 2209 22 4.151246801504147 2209 23 -0.2207283742695978 2209 24 -3.3658023233095165 2209 25 0.18256945586682605 2210 1 -1.956442745609035 2210 2 -13.721500646859223 2210 3 -7.51414786204068 2210 4 -2.0944107154995066 2210 5 -0.5568185119360081 2210 6 -0.7514027828104304 2210 7 -0.8137946727977834 2210 8 -20.677614724499758 2210 9 -12.85482284807376 2210 10 -6.749788587788732 2210 11 -10.34311969013618 2210 12 -4.455709694379163 2210 13 -5.63131319717346 2210 14 -3.9812649418848736 2210 15 -5.848644435692723 2210 16 -4.732077519373 2210 17 -4.4780184545088595 2210 18 -4.532923027994311 2210 19 -9.429783247537124 2210 20 -6.404730456149295 2210 21 -4.807884006116591 2210 22 -10.066924432918556 2210 23 -7.652722862075056 2210 24 -10.137794672763643 2210 25 -7.556504162817614 2211 1 0.8685030618596805 2211 2 5.4647738629447 2211 3 1.1262477434376912 2211 4 2.3805375552660877 2211 5 2.2183440656578455 2211 6 0.843421830451959 2211 7 -20.592689145159593 2211 8 -12.800163744107602 2211 9 -8.362249688156528 2211 10 -6.854872832541908 2211 11 -8.824103038249294 2211 12 -4.226044210094997 2211 13 -6.50248277053044 2211 14 -4.378373031351817 2211 15 -5.022627094082711 2211 16 -11.78932453878378 2211 17 -7.464753600828693 2211 18 -5.488103341325672 2211 19 -8.103716639989582 2211 20 -6.239911262140033 2211 21 -4.057157333538012 2211 22 -5.829388715113847 2211 23 -7.3691604421322054 2211 24 -21.715684119999825 2211 25 -21.53137853467691 2212 1 0.024110426756859482 2212 2 -13.721500646859223 2212 3 -7.220695505406562 2212 4 -19.516521268263325 2212 5 -19.73018534610686 2212 6 -20.482558561704902 2212 7 -9.365046868736828 2212 8 -8.588183830190815 2212 9 -3.9185425304967154 2212 10 -4.56784907995144 2212 11 -4.606264683395157 2212 12 -1.873162813633975 2212 13 -5.37909683078869 2212 14 -2.4699958593448024 2212 15 -2.4062154887901883 2212 16 -2.843365667430362 2212 17 -2.973939795631818 2212 18 -1.9113258823746122 2212 19 -2.585344250383333 2212 20 -2.890676446262461 2212 21 -0.5232222147750569 2212 22 -1.9028384201991315 2212 23 -2.067928973935171 2212 24 -0.9053860170187953 2212 25 -1.5422186430779945 2213 1 -18.398653800885416 2213 2 -13.721500646859223 2213 3 -7.752484431250366 2213 4 -4.059134904626962 2213 5 -19.73018534610686 2213 6 -9.709831288977629 2213 7 -20.592689145159593 2213 8 -9.615114724499758 2213 9 -6.78454246890519 2213 10 -5.887793955536562 2213 11 -4.8973539295148925 2213 12 -3.2802442992215575 2213 13 -18.875031790138284 2213 14 -11.167365901227038 2213 15 -4.978955079822462 2213 16 -4.237074093150984 2213 17 -2.8589715152671964 2213 18 -2.994620721004818 2213 19 -3.998992932324697 2213 20 -4.042607340571404 2213 21 -1.8725539467287433 2213 22 -2.6675126010318486 2213 23 -2.5816715829521684 2213 24 -1.4969341199998247 2213 25 -2.5853558074041842 2214 1 -18.398653800885416 2214 2 -13.721500646859223 2214 3 -12.630589201866746 2214 4 -19.516521268263325 2214 5 -19.73018534610686 2214 6 -20.482558561704902 2214 7 -16.766063758162687 2214 8 -15.327769523261368 2214 9 -14.46093389868284 2214 10 -14.543727321705996 2214 11 -14.80810716621627 2214 12 -13.072664437133183 2214 13 -18.875031790138284 2214 14 -17.042717716594332 2214 15 -23.54713689800428 2214 16 -18.542678511951994 2214 17 -23.236812424358106 2214 18 -18.025642041016077 2214 19 -15.46620374009278 2214 20 -14.274224987630229 2214 21 -12.002074774198485 2214 22 -12.871442378684538 2214 23 -12.848391608282876 2214 24 -11.67234046674905 2214 25 -11.472555005265146 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 44022.0 3 3.0 4 225.0 5 878.0 6 1211.0 7 1157.0 8 1036.0 9 925.0 10 843.0 11 731.0 12 781.0 13 815.0 14 1226.0 15 2416.0 16 3649.0 17 3896.0 18 3939.0 19 4601.0 20 5232.0 21 6365.0 22 6655.0 23 7296.0 24 7489.0 25 7885.0 26 6676.0 27 5256.0 28 5583.0 29 6436.0 30 6400.0 31 7291.0 32 9157.0 33 11628.0 34 14082.0 35 15995.0 36 15494.0 37 16095.0 38 10417.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.65465742589175 18.580737504237447 13.775000941655053 23.989604128215753 2 12.22095476009916 20.703675144501304 42.309089402812916 24.76628069258662 3 20.37699636117311 25.6315839752664 28.391100072081777 25.60031959147872 4 11.446618248917698 17.774360672801684 38.607556007664826 32.17146507061579 5 11.884877175628253 38.43613839233143 35.40403683553546 14.274947596504864 6 29.993123800246398 36.624836451498915 18.860842907486465 14.521196840768225 7 25.707041770615756 33.30461105371668 21.295389848553885 19.69295732711368 8 24.266591093260832 38.34139990878054 20.271975698426687 17.120033299531947 9 25.566478593309842 17.623048430942585 20.131240709258304 36.67923226648927 10 15.668736232390994 31.23045467984193 32.39515796574598 20.705651122021095 11 31.427070124546198 24.408535843943792 23.610363216314337 20.554030815195677 12 24.260203305562435 27.07466850936779 30.045589468789252 18.619538716280527 13 28.10452130753422 22.56582263755865 24.336441893830703 24.993214161076427 14 20.25761417086433 25.22934249591353 26.876021616572704 27.63702171664943 15 24.141733347979283 30.828735350531055 23.20509679000245 21.824434511487212 16 19.65798231555445 29.669864977439858 30.012812977050817 20.65933972995488 17 21.46537597351038 29.715929248390854 28.81928606423057 19.999408713868196 18 20.787825499943743 28.10852706717727 31.536772274009593 19.566875158869394 19 22.63464974200722 28.242608996222767 27.885708574355643 21.237032687414366 20 22.772077638908318 28.98212067107136 28.04331191324453 20.2024897767758 21 20.854134616620744 27.532685690288357 27.701524260902726 23.911655432188173 22 21.406527595899608 29.913838403216253 26.93279729087254 21.746836710011593 23 20.294681685467545 29.39671562970963 28.53312089094396 21.775481793878868 24 21.694580748070347 29.34876155201391 28.2623878592896 20.694269840626138 25 21.702456192224453 28.128965491751778 27.66450245783489 22.504075858188884 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 610.0 1 610.0 2 998.5 3 1387.0 4 1387.0 5 1387.0 6 1694.5 7 2002.0 8 2002.0 9 2002.0 10 2317.5 11 2633.0 12 2633.0 13 2633.0 14 3023.5 15 3414.0 16 3414.0 17 3414.0 18 4600.0 19 5786.0 20 5786.0 21 5786.0 22 7832.0 23 9878.0 24 9878.0 25 9878.0 26 12844.5 27 15811.0 28 15811.0 29 15811.0 30 19044.0 31 22277.0 32 22277.0 33 22277.0 34 23940.0 35 25603.0 36 25603.0 37 25603.0 38 26531.0 39 27459.0 40 27459.0 41 27459.0 42 28443.0 43 29427.0 44 29427.0 45 29427.0 46 31768.5 47 34110.0 48 34110.0 49 34110.0 50 29904.5 51 25699.0 52 25699.0 53 25699.0 54 20999.0 55 16299.0 56 16299.0 57 16299.0 58 13191.5 59 10084.0 60 10084.0 61 10084.0 62 8029.5 63 5975.0 64 5975.0 65 5975.0 66 4514.0 67 3053.0 68 3053.0 69 3053.0 70 2229.5 71 1406.0 72 1406.0 73 1406.0 74 990.5 75 575.0 76 575.0 77 575.0 78 393.5 79 212.0 80 212.0 81 212.0 82 136.0 83 60.0 84 60.0 85 60.0 86 40.0 87 20.0 88 20.0 89 20.0 90 12.0 91 4.0 92 4.0 93 4.0 94 3.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.877687808159616 2 35.63248094640381 3 5.5343621044686735 4 13.303060881264717 5 12.917476803425956 6 14.097610199109054 7 9.07476229151797 8 7.365886474202784 9 0.1082916984568433 10 1.4927026162289878 11 5.541745629363458 12 5.615170682483818 13 15.371678439286915 14 1.62847743512753 15 2.9074680252352474 16 2.997300911455129 17 2.8771135340011322 18 1.5624359069019549 19 1.5025473160887008 20 0.2920594291714865 21 4.277111893217822 22 1.6424240932621232 23 0.11034267759428351 24 8.964829809751174 25 0.11444463586916395 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 243786.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.15899762697474 #Duplication Level Percentage of deduplicated Percentage of total 1 97.3186419381864 85.7951392368898 2 1.6769056201140502 2.9566863716859024 3 0.3576754413131779 0.945969251458668 4 0.16783798847377193 0.591857153103019 5 0.10139561640134663 0.44694679528559794 6 0.0641152793085915 0.3391403253851364 7 0.04503620577769788 0.277924273079881 8 0.03708039076199336 0.2615176064955095 9 0.032169770755554196 0.2552449269329301 >10 0.16181439042424273 2.7361314671803827 >50 0.01722801160763017 1.089320148141677 >100 0.018663679241599347 3.1502531162563527 >500 9.571117559794538E-4 0.6737584339977761 >1k 4.785558779897269E-4 0.4801108941073696 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 1138 0.4668028516813927 No Hit CCCATGTACTCTGCGTTGATACCAC 960 0.393787994388521 No Hit GAGTACATGGGAAGCAGTGGTATCA 637 0.26129474210988324 No Hit CATGTACTCTGCGTTGATACCACTG 434 0.17802498912981057 No Hit GCGTTGATACCACTGCTTCCCATGT 432 0.1772045974748345 No Hit GCTTCCCATGTACTCTGCGTTGATA 373 0.15300304365303996 No Hit GTATCAACGCAGAGTACATGGGAAG 367 0.1505418686881117 No Hit ACGCAGAGTACATGGGAAGCAGTGG 365 0.14972147703313562 No Hit GTCCTACAGTGGACATTTCTAAATT 298 0.12223835659143675 No Hit ACTCTGCGTTGATACCACTGCTTCC 280 0.11485483169665198 No Hit GTATCAACGCAGAGTACTTTTTTTT 267 0.10952228593930743 No Hit GNACATGGGAAGCAGTGGTATCAAC 257 0.10542032766442701 No Hit TATCAACGCAGAGTACATGGGAAGC 250 0.1025489568720107 No Hit GGTATCAACGCAGAGTACATGGGAA 249 0.10213876104452266 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTTAG 20 0.009533607 26.425934 1 ATAAGAC 30 0.007914177 18.115381 3 TAGGACC 80 3.068817E-8 15.995341 4 CCAACGA 40 6.01568E-4 14.997783 19 GTCCTAC 190 0.0 14.603805 1 TTAGGAC 60 1.29717E-4 14.492305 3 CCACACT 45 0.0053330273 14.218081 4 TCCTACA 220 0.0 14.145201 2 CTGTAGG 255 0.0 13.990199 1 TGTAGGA 285 0.0 13.767564 2 TCCAACG 50 2.0590733E-4 13.728073 18 TCTGGAC 50 0.009436896 13.043074 3 TGTCCAC 50 0.0019353725 12.84784 10 GGACGTG 210 0.0 12.82025 6 AGGACCT 155 5.456968E-12 12.700072 5 GTAGGAC 310 0.0 12.62233 3 GACGTGG 130 9.0221874E-10 12.487138 7 GGACCTG 150 3.8198777E-11 12.425779 6 TAGGACG 215 0.0 12.399488 4 AGGACGT 220 0.0 12.2443285 5 >>END_MODULE