FastQCFastQC Report
Thu 26 May 2016
SRR1926563_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926563_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243786
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC29161.196131032955133No Hit
CCCATGTACTCTGCGTTGATACCAC27481.1272181339371417No Hit
GAGTACATGGGAAGCAGTGGTATCA16050.6583643031183086No Hit
CATGTACTCTGCGTTGATACCACTG11700.4799291181610101No Hit
GTATCAACGCAGAGTACTTTTTTTT10690.4384993395847177No Hit
GTATCAACGCAGAGTACATGGGAAG10610.4352177729648134No Hit
GCGTTGATACCACTGCTTCCCATGT10060.41265700245297104No Hit
ACGCAGAGTACATGGGAAGCAGTGG9600.393787994388521No Hit
GCTTCCCATGTACTCTGCGTTGATA8360.3429237117800038No Hit
GGTATCAACGCAGAGTACTTTTTTT7540.30928765392598423No Hit
TATCAACGCAGAGTACATGGGAAGC7050.2891880583790702No Hit
ACTCTGCGTTGATACCACTGCTTCC6930.28426570844921367No Hit
GGTATCAACGCAGAGTACATGGGAA6550.26867826700466807No Hit
CTGTAGGACGTGGAATATGGCAAGA6420.26334572124732347No Hit
GTCCTACAGTGGACATTTCTAAATT6300.25842337131746695No Hit
TATCAACGCAGAGTACTTTTTTTTT4960.2034571304340692No Hit
CTTTAGGACGTGAAATATGGCGAGG4440.182126947404691No Hit
GATATACACTGTTCTACAAATCCCG4410.18089635992222686No Hit
CAGTGGTATCAACGCAGAGTACATG4320.1772045974748345No Hit
CATGGGAAGCAGTGGTATCAACGCA4260.1747434225099062No Hit
ACATGGGAAGCAGTGGTATCAACGC4170.17105166006251385No Hit
GTGGTATCAACGCAGAGTACATGGG4080.16735989761512146No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4040.16571911430516928No Hit
GTCCTAAAGTGTGTATTTCTCATTT3770.15464382696299211No Hit
GTCCTACAGTGTGCATTTCTCATTT3460.14192775631086282No Hit
CCATGTACTCTGCGTTGATACCACT3430.14069716882839867No Hit
GGGAAGCAGTGGTATCAACGCAGAG3260.13372383976110194No Hit
ATACCACTGCTTCCCATGTACTCTG3250.1333136439336139No Hit
GCAGTGGTATCAACGCAGAGTACAT3210.13167286062366174No Hit
AAGCAGTGGTATCAACGCAGAGTAC2890.11854659414404438No Hit
GTGTATATCAATGAGTTACAATGAG2880.11813639831655633No Hit
CTGTAGGACCTGGAATATGGCGAGA2870.11772620248906827No Hit
GTGTATATCAATGAGTTACAATGAA2700.11075287342177155No Hit
CCACTGCTTCCCATGTACTCTGCGT2690.11034267759428351No Hit
CTGCTTCCCATGTACTCTGCGTTGA2670.10952228593930743No Hit
CTGAAGGACCTGGAATATGGCGAGA2640.1082916984568433No Hit
GTACTCTGCGTTGATACCACTGCTT2550.1045999360094509No Hit
GCAGAGTACATGGGAAGCAGTGGTA2530.10377954435447483No Hit
GATACCACTGCTTCCCATGTACTCT2470.10131836938954658No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAA250.005875681519.078721
CTTCTAA250.005875681519.078721
TAATACT402.6594938E-416.697334
GCATTTT350.00219589616.24911113
TCTGCAC400.00510565614.3149563
TCTAAAG400.00510565614.3149563
TAGGACT1051.2605597E-913.6304724
GGCGAGG1400.013.52981719
ACCTGTA500.001497725413.2946738
TTTAGGA2200.013.0162852
GATATAC1550.012.92429351
CTGTTCT2150.012.8088219
TAGGACA603.923511E-412.7217744
TATACAC1700.012.3501573
AATCCCG1001.4811849E-712.31213419
TTAGGAC2250.012.3002573
CTTTAGG2250.012.2951741
TACACTG2850.012.0522075
ACTGTTC2050.012.0439558
TCCTACA4050.012.0199792
Produced by FastQC (version 0.11.5)