FastQCFastQC Report
Thu 26 May 2016
SRR1926562_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926562_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662849
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC30100.45410040597481477No Hit
CCCATGTACTCTGCGTTGATACCAC27560.4157809697231195No Hit
GAGTACATGGGAAGCAGTGGTATCA13940.21030430761757204No Hit
GCGTTGATACCACTGCTTCCCATGT10150.15312688108453057No Hit
CATGTACTCTGCGTTGATACCACTG9670.14588541281649364No Hit
GTATCAACGCAGAGTACATGGGAAG8660.13064815666916596No Hit
ACGCAGAGTACATGGGAAGCAGTGG7930.11963509034485983No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGC250.005205580519.4877978
GATATAC1650.018.559221
GTCCAAG552.3808835E-416.9453741
GATCATC403.5728293E-416.06910711
GTCAGTA603.8326104E-415.9193342
GTTATAC505.236403E-415.2804973
GTCCTAA1850.015.11344051
TGTAGGA3450.014.6339242
GTCCTAT550.004642341814.5246061
GTCTAGG751.548667E-414.2018381
GTAGGAC3750.013.970743
GTCCTAC4100.013.96369651
GCCCTAT600.00826203613.3142211
GTGTAGG600.00826203613.3142211
GCTTTAA600.00826203613.3142211
AGGCTGT550.001549659113.2390636
CTCTTCG701.0286085E-513.1176389
TCCTACA4800.013.0765962
ACTGTTC2700.012.9918648
CTGTAGG3300.012.9107611
Produced by FastQC (version 0.11.5)