FastQCFastQC Report
Thu 26 May 2016
SRR1926562_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926562_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662849
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC79181.194540536381589No Hit
GTACATGGGAAGCAGTGGTATCAAC74921.1302725055027616No Hit
GAGTACATGGGAAGCAGTGGTATCA34050.5136916552638686No Hit
CATGTACTCTGCGTTGATACCACTG28550.4307164980259456No Hit
GCGTTGATACCACTGCTTCCCATGT22750.3432154231204995No Hit
ACGCAGAGTACATGGGAAGCAGTGG20900.31530559750410725No Hit
GTATCAACGCAGAGTACATGGGAAG19890.3000683413567796No Hit
GTATCAACGCAGAGTACTTTTTTTT17320.2612963133383319No Hit
ACTCTGCGTTGATACCACTGCTTCC16580.25013238309177505No Hit
GCTTCCCATGTACTCTGCGTTGATA14340.21633886450760278No Hit
TATCAACGCAGAGTACATGGGAAGC13860.20909739623956586No Hit
GTCCTACAGTGGACATTTCTAAATT12960.19551964323699664No Hit
GGTATCAACGCAGAGTACATGGGAA12260.18495916867944281No Hit
CTGTAGGACGTGGAATATGGCAAGA10930.16489426702009055No Hit
CAGTGGTATCAACGCAGAGTACATG10500.15840711836330748No Hit
GGTATCAACGCAGAGTACTTTTTTT10390.15674761521854902No Hit
CTTTAGGACGTGAAATATGGCGAGG8710.13140247628041982No Hit
TATCAACGCAGAGTACTTTTTTTTT8570.12929038136890905No Hit
CATGGGAAGCAGTGGTATCAACGCA8560.1291395174466583No Hit
GTCCTAAAGTGTGTATTTCTCATTT8420.1270274225351475No Hit
GCAGTGGTATCAACGCAGAGTACAT8010.120842001722866No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7830.11812645112235215No Hit
GTCCTACAGTGTGCATTTCTCATTT7830.11812645112235215No Hit
GTGGTATCAACGCAGAGTACATGGG7750.11691953974434599No Hit
ATACCACTGCTTCCCATGTACTCTG7450.11239362207682294No Hit
ACATGGGAAGCAGTGGTATCAACGC7150.10786770440929985No Hit
GATATACACTGTTCTACAAATCCCG6890.10394524243077988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCGT250.006025579319.0015898
ATTATGC350.002107659616.355143
CCGGTTT551.1490874E-515.51271412
CGGTTTC508.859792E-515.16798713
ATTGTAT456.5405975E-414.84077553
GCTCTAG456.56703E-414.8328871
AATCCCG1500.014.52392619
GTAGCAC400.0051280314.3107483
TGCCGGT602.6134858E-514.22106310
GCCGGTT602.6153473E-514.21998811
TAGGACC2050.013.4942654
GCTCTAC655.2563613E-513.2028981
CAAATCC1850.012.80113617
GATATAC3600.012.7139021
GTATAAG751.4201174E-512.7139021
GTAGGAC5950.012.6672153
TCCAACG751.5208074E-512.62950118
CCAACGA604.1890738E-412.62950119
CCCTACA701.04517E-412.2682192
TGTAGGA6300.012.2682192
Produced by FastQC (version 0.11.5)